Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,485 T→G 8.9% intergenic (+92/+623) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,4850TG 8.9% 139.6 / 3.7 54intergenic (+92/+623)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (13/36);  new base G (2/3);  total (15/39)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGC  >  NC_000913/2304337‑2304634
                                                                                                                                                    |                                                                                                                                                     
gtgAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTg                                                                                                                                                      >  2:1777033/1‑150 (MQ=255)
 tgAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                     >  2:372492/1‑150 (MQ=255)
  gAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGGCATCCCACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                                                    >  1:2173283/1‑150 (MQ=255)
    aGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                                                  >  1:170286/1‑150 (MQ=255)
     gTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                                                 <  2:1208178/150‑1 (MQ=255)
      tACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                                                <  2:2098491/150‑1 (MQ=255)
       aCCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                                               <  2:511932/150‑1 (MQ=255)
        ccGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGAGGCGCGGGCGCGACt                                                                                                                                              >  1:2134789/1‑150 (MQ=255)
         cgcgTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                                             <  2:1906330/150‑1 (MQ=255)
           cgTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                           <  2:198030/150‑1 (MQ=255)
            gTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCNGNCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                          <  2:990602/150‑1 (MQ=255)
            gTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                          <  2:1705143/150‑1 (MQ=255)
                ggAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                                                                      <  2:870971/150‑1 (MQ=21)
                 gAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                                                                                                     <  1:1784685/150‑1 (MQ=255)
                  aaGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt                                                                                                                                    <  2:2198417/150‑1 (MQ=255)
                  aaGGCGGAAGAGAAAATTCACAACGCGGTAGCTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATAAGACGCGCCAGCGTCGCATCAGGcaa                                                                                                                                    <  1:2203689/150‑3 (MQ=18)
                   aGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGATAAGACGCGCCAGCGTCGCATCAGGcaac                                                                                                                                   <  1:2279713/150‑4 (MQ=18)
                       ggAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                               >  2:1702616/1‑150 (MQ=255)
                       ggAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                               <  2:656597/150‑1 (MQ=255)
                          agagAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                                                                                                            <  2:972163/150‑1 (MQ=255)
                          agagAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACCCCGCt                                                                                                                            >  1:1211979/1‑150 (MQ=255)
                           gagaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                                                                                           <  2:608449/150‑1 (MQ=255)
                            agaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCAGGCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                          >  1:244149/1‑150 (MQ=255)
                            agaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                          >  1:1482918/1‑150 (MQ=255)
                            agaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                          <  2:2026214/150‑1 (MQ=255)
                                aaTTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                                                                                                      <  1:1553769/150‑1 (MQ=255)
                                  ttGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTg                                                                                                                    >  1:2138063/1‑150 (MQ=255)
                                   tGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc                                                                                                                   <  1:199915/150‑1 (MQ=255)
                                    gACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCg                                                                                                                  <  1:372492/150‑1 (MQ=255)
                                    gACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCg                                                                                                                  <  2:54905/150‑1 (MQ=255)
                                     aCAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCgg                                                                                                                 <  1:1702616/150‑1 (MQ=255)
                                      cAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTGACGCCGCATCCGAAGTCCAGAGCCCCAGCCGGTTGCCTGAAGCGACGCGGGCGCGGCGGATCAGGCCGAGCCCGATGTGAAGTGCgcc                                                                                                                >  1:1795734/1‑148 (MQ=17)
                                      cAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGc                                                                                                                <  1:84227/150‑1 (MQ=255)
                                         cgcgGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAcc                                                                                                             <  2:516508/150‑1 (MQ=255)
                                            ggTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                          >  1:698928/1‑150 (MQ=255)
                                              tAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCTGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                        <  2:1649423/150‑1 (MQ=255)
                                              tAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                        <  2:77562/150‑1 (MQ=255)
                                              tAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCACGTTGCCGGATCCGACGCTGCGCCGTGGTCTCAGGGCTACCCAGCTGTGAAGTGAGGCACCCCgaa                                                                                                        >  1:2409160/1‑148 (MQ=14)
                                                tttCGCTAAACTGCCGTGAAGTGCGGCAGCCGGTAGGTCAGACAATGCGGTCAGGCCGCATCCTACATAAAACGCCCGAGTCGTTTGCGTGATTCGACGATGTCTCGGCTTATCAGGGCTACACGGCTGTGAAGTGCGGCACCCCGTAgg                                                                                                      <  2:1255050/149‑1 (MQ=255)
                                                   ggCTAAACTGCCGTTAAGTGCGCCACCCGGTAGGGCTGATCATGAGGTCACTCCTCATCCGACATCACACGCCCTAGCCGGTGGCCTTATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGttg                                                                                                   <  2:2105072/149‑3 (MQ=2)
                                                           tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGc                                                                                           <  1:1458978/150‑1 (MQ=255)
                                                           tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCACATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGc                                                                                           <  1:173651/150‑1 (MQ=255)
                                                            gCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCg                                                                                          <  1:1211955/150‑1 (MQ=255)
                                                              cGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGGCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGt                                                                                        <  2:1213216/150‑1 (MQ=255)
                                                               gTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGGCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTc                                                                                       <  1:2372896/150‑1 (MQ=255)
                                                               gTGAAGTGCGGAACCCCGTAGGTCAGACTAGTCGGTCACTCCGCATAGGACATCCAACGCGCGAGCCGTTTGCGTTATGCTAATCTGGCGCGTCTTATCAGGGCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTc                                                                                       <  2:1775155/150‑1 (MQ=25)
                                                                     tGCGGCACCCCGTCGGTCAGTCAAGGCGGGCACCCAGCATCCGTCATCCTACGCCCGAGCCGCTGGCGGGATGCTACGCTGTCGCGTCTTATCATGGCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccg                                                                                 <  2:1419950/150‑1 (MQ=25)
                                                                     tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACTTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccg                                                                                 <  1:1943300/150‑1 (MQ=255)
                                                                                   ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCaa                                                                   >  2:23703/1‑150 (MQ=255)
                                                                                   cgTCAGACCAGGCGGCCACCCCGCCTCCCACATCCCACGCCCGAGCCGGAGGCCGCAGGCCACCCTGCCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCaa                                                                   <  1:1661436/149‑1 (MQ=14)
                                                                                        gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCAGACAAGGCGCTAACGCCGCATCCGACATCAAACGccc                                                              >  1:1180224/1‑150 (MQ=255)
                                                                                                     cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTTATCCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCt                                                 <  1:86925/150‑1 (MQ=255)
                                                                                                         gcATCCCACAGACCACGCCCGAGCGGCTTGCCTTAGCCTACCCTGCCGCGGCTTATGAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATg                                             <  2:265443/150‑1 (MQ=14)
                                                                                                                                                  gCTGGCGCGGCGTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGt    <  2:333777/150‑1 (MQ=255)
                                                                                                                                                    tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc  <  2:11094/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGC  >  NC_000913/2304337‑2304634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑