Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,494 T→G 14.3% intergenic (+101/+614) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,4940TG14.3% 95.3 / 4.0 47intergenic (+101/+614)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (7/33);  new base G (3/4);  total (10/37)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAC  >  NC_000913/2304345‑2304639
                                                                                                                                                     |                                                                                                                                                 
ccGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGAGGCGCGGGCGCGACt                                                                                                                                                   >  1:2134789/1‑150 (MQ=255)
 cgcgTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                                                  <  2:1906330/150‑1 (MQ=255)
   cgTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                <  2:198030/150‑1 (MQ=255)
    gTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCNGNCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                               <  2:990602/150‑1 (MQ=255)
    gTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                               <  2:1705143/150‑1 (MQ=255)
        ggAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                                                                           <  2:870971/150‑1 (MQ=21)
         gAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                                                                                                          <  1:1784685/150‑1 (MQ=255)
          aaGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt                                                                                                                                         <  2:2198417/150‑1 (MQ=255)
          aaGGCGGAAGAGAAAATTCACAACGCGGTAGCTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATAAGACGCGCCAGCGTCGCATCAGGcaa                                                                                                                                         <  1:2203689/150‑3 (MQ=18)
           aGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGATAAGACGCGCCAGCGTCGCATCAGGcaac                                                                                                                                        <  1:2279713/150‑4 (MQ=18)
               ggAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                    >  2:1702616/1‑150 (MQ=255)
               ggAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                    <  2:656597/150‑1 (MQ=255)
                  agagAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                                                                                                                 <  2:972163/150‑1 (MQ=255)
                  agagAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACCCCGCt                                                                                                                                 >  1:1211979/1‑150 (MQ=255)
                   gagaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                                                                                                <  2:608449/150‑1 (MQ=255)
                    agaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCAGGCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                               >  1:244149/1‑150 (MQ=255)
                    agaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                               <  2:2026214/150‑1 (MQ=255)
                    agaAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                               >  1:1482918/1‑150 (MQ=255)
                        aaTTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                                                                                                           <  1:1553769/150‑1 (MQ=255)
                          ttGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTg                                                                                                                         >  1:2138063/1‑150 (MQ=255)
                           tGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc                                                                                                                        <  1:199915/150‑1 (MQ=255)
                            gACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCg                                                                                                                       <  1:372492/150‑1 (MQ=255)
                            gACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCg                                                                                                                       <  2:54905/150‑1 (MQ=255)
                             aCAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCgg                                                                                                                      <  1:1702616/150‑1 (MQ=255)
                              cAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTGACGCCGCATCCGAAGTCCAGAGCCCCAGCCGGTTGCCTGAAGCGACGCGGGCGCGGCGGATCAGGCCGAGCCCGATGTGAAGTGCgcc                                                                                                                     >  1:1795734/1‑148 (MQ=17)
                              cAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGc                                                                                                                     <  1:84227/150‑1 (MQ=255)
                                 cgcgGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAcc                                                                                                                  <  2:516508/150‑1 (MQ=255)
                                    ggTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                               >  1:698928/1‑150 (MQ=255)
                                      tAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCTGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                             <  2:1649423/150‑1 (MQ=255)
                                      tAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                             <  2:77562/150‑1 (MQ=255)
                                      tAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCACGTTGCCGGATCCGACGCTGCGCCGTGGTCTCAGGGCTACCCAGCTGTGAAGTGAGGCACCCCgaa                                                                                                             >  1:2409160/1‑148 (MQ=14)
                                        tttCGCTAAACTGCCGTGAAGTGCGGCAGCCGGTAGGTCAGACAATGCGGTCAGGCCGCATCCTACATAAAACGCCCGAGTCGTTTGCGTGATTCGACGATGTCTCGGCTTATCAGGGCTACACGGCTGTGAAGTGCGGCACCCCGTAgg                                                                                                           <  2:1255050/149‑1 (MQ=255)
                                           ggCTAAACTGCCGTTAAGTGCGCCACCCGGTAGGGCTGATCATGAGGTCACTCCTCATCCGACATCACACGCCCTAGCCGGTGGCCTTATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGttg                                                                                                        <  2:2105072/149‑3 (MQ=2)
                                                   tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGc                                                                                                <  1:1458978/150‑1 (MQ=255)
                                                   tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCACATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGc                                                                                                <  1:173651/150‑1 (MQ=255)
                                                    gCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCg                                                                                               <  1:1211955/150‑1 (MQ=255)
                                                      cGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGGCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGt                                                                                             <  2:1213216/150‑1 (MQ=255)
                                                       gTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGGCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTc                                                                                            <  1:2372896/150‑1 (MQ=255)
                                                       gTGAAGTGCGGAACCCCGTAGGTCAGACTAGTCGGTCACTCCGCATAGGACATCCAACGCGCGAGCCGTTTGCGTTATGCTAATCTGGCGCGTCTTATCAGGGCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTc                                                                                            <  2:1775155/150‑1 (MQ=25)
                                                             tGCGGCACCCCGTCGGTCAGTCAAGGCGGGCACCCAGCATCCGTCATCCTACGCCCGAGCCGCTGGCGGGATGCTACGCTGTCGCGTCTTATCATGGCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccg                                                                                      <  2:1419950/150‑1 (MQ=25)
                                                             tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACTTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccg                                                                                      <  1:1943300/150‑1 (MQ=255)
                                                                           ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCaa                                                                        >  2:23703/1‑150 (MQ=255)
                                                                           cgTCAGACCAGGCGGCCACCCCGCCTCCCACATCCCACGCCCGAGCCGGAGGCCGCAGGCCACCCTGCCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCaa                                                                        <  1:1661436/149‑1 (MQ=14)
                                                                                gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCAGACAAGGCGCTAACGCCGCATCCGACATCAAACGccc                                                                   >  1:1180224/1‑150 (MQ=255)
                                                                                             cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTTATCCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCt                                                      <  1:86925/150‑1 (MQ=255)
                                                                                                 gcATCCCACAGACCACGCCCGAGCGGCTTGCCTTAGCCTACCCTGCCGCGGCTTATGAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATg                                                  <  2:265443/150‑1 (MQ=14)
                                                                                                                                          gCTGGCGCGGCGTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGt         <  2:333777/150‑1 (MQ=255)
                                                                                                                                            tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc       <  2:11094/150‑1 (MQ=255)
                                                                                                                                                 cgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCAc  <  2:2245443/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAC  >  NC_000913/2304345‑2304639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑