BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,491,266	C→G	5.1%	E229Q (GAA→CAA)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,491,266	0	C	G	5.1%	467.3 / 10.6	136	E229Q (GAA→CAA)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand): ref base C (68/61); new base G (4/3); total (72/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 141 total aligned reads displayed.

ACCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACG  >  NC_000913/2491117‑2491412

aCCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtc                                                                                                                                                     <  2:936858/150‑1 (MQ=255)

 ccAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                                    >  2:2348549/1‑150 (MQ=255)

 ccAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                                    >  1:81191/1‑150 (MQ=255)

      aaGCATGACAACGTCAGCATTTG‑CGGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCt                                                                                                                                              <  1:1214443/150‑1 (MQ=255)

           tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                                         <  2:612301/150‑1 (MQ=255)

           tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                                         <  2:2429788/150‑1 (MQ=255)

           tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCAC‑AAGCTGATGAACAGCttcgtg                                                                                                                                         <  1:2006138/150‑5 (MQ=255)

            gACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGa                                                                                                                                        <  2:746872/150‑1 (MQ=255)

             aCAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGaa                                                                                                                                       >  2:270036/1‑150 (MQ=255)

              cAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGCCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCACCTT‑GTGaaa                                                                                                                                      >  2:1562412/1‑149 (MQ=255)

               aaCGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATa                                                                                                                                     <  1:243178/150‑1 (MQ=255)

                aCGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTTTCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGATTGGAATCTGAGGACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAc                                                                                                                                    <  2:1276671/150‑1 (MQ=255)

                aCGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAc                                                                                                                                    <  2:1085443/150‑1 (MQ=255)

                  gTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgc                                                                                                                                  >  2:414024/1‑150 (MQ=255)

                   cgttcgtttGCCCTGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgcc                                                                                                                                 >  1:1712261/7‑150 (MQ=255)

                      gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCcgcg                                                                                                                              >  2:2045865/1‑150 (MQ=255)

                        aTTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGcc                                                                                                                            >  1:616607/1‑150 (MQ=255)

                           tGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCttt                                                                                                                         >  1:987546/1‑150 (MQ=255)

                           tGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCttt                                                                                                                         >  2:1486391/1‑150 (MQ=255)

                            gCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTg                                                                                                                        <  2:1907680/150‑1 (MQ=255)

                             ccAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGc                                                                                                                       <  2:1476128/150‑1 (MQ=255)

                                gggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCATTTCGCCATAGCCCTTGGNNGNAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTG‑GTGAATACGCCGCGCCTTTGCCaa                                                                                                                    >  2:73561/1‑150 (MQ=255)

                                 ggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAg                                                                                                                   <  1:1671553/150‑1 (MQ=255)

                                 ggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAg                                                                                                                   >  2:168605/1‑150 (MQ=255)

                                   cAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCACCATAGACATTGGCAGGAATGGAATCTGCGCACTTTCAATAAATTAACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGa                                                                                                                 >  2:247295/1‑150 (MQ=255)

                                    aaaCGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAt                                                                                                                <  2:532520/150‑1 (MQ=255)

                                    aaaCGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAt                                                                                                                >  1:1696053/1‑150 (MQ=255)

                                     aacgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAta                                                                                                               >  1:94440/1‑150 (MQ=255)

                                        gaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaatt                                                                                                            <  2:2057347/150‑1 (MQ=255)

                                                  cTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGtt                                                                                                  <  2:491565/150‑1 (MQ=255)

                                                   tGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTc                                                                                                 >  1:886711/1‑150 (MQ=255)

                                                   tGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTc                                                                                                 >  2:2377954/1‑150 (MQ=255)

                                                    gCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCa                                                                                                >  1:143249/1‑150 (MQ=255)

                                                      cGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGc                                                                                              >  2:190972/1‑150 (MQ=255)

                                                       gCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCt                                                                                             >  1:218169/1‑150 (MQ=255)

                                                       gCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCt                                                                                             >  1:805963/1‑150 (MQ=255)

                                                        cAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCt                                                                                             >  1:2423123/1‑149 (MQ=255)

                                                        cAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCt                                                                                             <  2:2423123/149‑1 (MQ=255)

                                                          gAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCtttt                                                                                          <  1:442180/150‑1 (MQ=255)

                                                            aaGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGc                                                                                        >  2:1730421/1‑150 (MQ=255)

                                                              gTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTa                                                                                      <  1:2304090/150‑1 (MQ=255)

                                                                ggATGCGTATCTTCAAGGATCCCTTTCGCCATAGATATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaa                                                                                    <  2:964383/150‑1 (MQ=255)

                                                                  aTGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGGAGGAATGGAATCTGACCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAg                                                                                  <  1:1084352/150‑1 (MQ=255)

                                                                  aTGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAg                                                                                  >  2:1239723/1‑150 (MQ=255)

                                                                      gTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGaa                                                                              >  1:1264079/1‑150 (MQ=255)

                                                                      gTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGaa                                                                              >  1:2404729/1‑150 (MQ=255)

                                                                         tCTTCAATGATCACTTTCGACATAGAAATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTAACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGACTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAAtt                                                                           >  2:1873463/1‑150 (MQ=255)

                                                                         tCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAAtt                                                                           <  2:829908/150‑1 (MQ=255)

                                                                            tCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgcg                                                                        >  1:1822468/1‑150 (MQ=255)

                                                                             cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTTAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgcgc                                                                       <  2:530145/150‑1 (MQ=255)

                                                                             cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgcgc                                                                       <  1:1020103/150‑1 (MQ=255)

                                                                              aaGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCt                                                                      <  1:1702823/150‑1 (MQ=255)

                                                                                ggATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAg                                                                    >  2:1092231/1‑150 (MQ=255)

                                                                                 gATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGt                                                                   <  1:1577115/150‑1 (MQ=255)

                                                                                  aTCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtg                                                                  <  2:1213046/150‑1 (MQ=255)

                                                                                  aTCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtg                                                                  <  1:1800023/150‑1 (MQ=255)

                                                                                  aTCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtg                                                                  <  2:1645765/150‑1 (MQ=255)

                                                                                     ccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgact                                                               <  1:2377954/150‑1 (MQ=255)

                                                                                       tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactga                                                             >  2:1259517/1‑150 (MQ=255)

                                                                                       tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactga                                                             <  1:21008/150‑1 (MQ=255)

                                                                                        ttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactgac                                                            >  2:1435584/1‑150 (MQ=255)

                                                                                          cGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACtt                                                          >  1:692521/1‑150 (MQ=255)

                                                                                            ccATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCg                                                        >  1:2381712/1‑150 (MQ=255)

                                                                                             cATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCgg                                                       <  1:1259517/150‑1 (MQ=255)

                                                                                                     ttGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCa                                               <  1:1108230/150‑1 (MQ=255)

                                                                                                       ggCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCaat                                             >  1:521200/1‑150 (MQ=255)

                                                                                                         cAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCACCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCaataa                                           >  2:1555184/1‑150 (MQ=255)

                                                                                                           ggaatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATg                                         >  2:972539/1‑150 (MQ=255)

                                                                                                            gaatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGc                                        <  1:526897/150‑1 (MQ=255)

                                                                                                            gaatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGc                                        <  2:94440/150‑1 (MQ=255)

                                                                                                              atggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTg                                      <  1:2348549/150‑1 (MQ=255)

                                                                                                               tggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTgg                                     <  1:1329970/150‑1 (MQ=255)

                                                                                                                ggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGc                                    >  1:120710/1‑150 (MQ=255)

                                                                                                                ggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGc                                    <  1:657598/150‑1 (MQ=255)

                                                                                                                ggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGc                                    >  2:588800/1‑150 (MQ=255)

                                                                                                                ggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGc                                    >  2:1209360/1‑150 (MQ=255)

                                                                                                                 gaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGATCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCg                                   <  2:1256423/150‑1 (MQ=255)

                                                                                                                    tCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACg                                <  1:849432/150‑1 (MQ=255)

                                                                                                                    ccTGAGCACTATCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACg                                <  1:425838/149‑1 (MQ=255)

                                                                                                                     cTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACgg                               >  1:1106661/1‑150 (MQ=255)

                                                                                                                      tGAGCACTTCCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGa                              >  1:311276/1‑150 (MQ=255)

                                                                                                                       gAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAt                             <  2:327081/150‑1 (MQ=255)

                                                                                                                       gAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAt                             >  1:694577/1‑150 (MQ=255)

                                                                                                                          cACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAtttt                          >  1:619489/1‑150 (MQ=255)

                                                                                                                              ttCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAAc                      <  1:1274534/150‑1 (MQ=255)

                                                                                                                                cggcgtaTTCAC‑AAGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACtt                    >  1:1240775/7‑150 (MQ=38)

                                                                                                                                 aaTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttt                   >  1:2058133/1‑150 (MQ=255)

                                                                                                                                   tAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTaa                 >  1:1027325/1‑150 (MQ=255)

                                                                                                                                   cgtaTTCAC‑AAGCTGATGAACAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGTATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAAc                >  2:1502273/4‑150 (MQ=255)

                                                                                                                                    aaaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAAc                >  1:999526/1‑150 (MQ=255)

                                                                                                                                    aaaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAAAAATGCTGGCGACGGATTTTCAACTTTAAc                <  1:2201441/150‑1 (MQ=255)

                                                                                                                                       ttCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAAt             <  1:955679/150‑1 (MQ=255)

                                                                                                                                        tCACGAAGCTGTTCATCAGCTT‑GTGAATAAGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATc            >  1:910346/1‑150 (MQ=255)

                                                                                                                                         cACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg           >  2:2405972/1‑150 (MQ=255)

                                                                                                                                         cACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg           <  2:65813/150‑1 (MQ=255)

                                                                                                                                          cacaaGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg           >  2:101823/4‑150 (MQ=255)

                                                                                                                                            gAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTgg        <  2:805963/150‑1 (MQ=255)

                                                                                                                                               gCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCT‑AAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATATTGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTaa    <  2:81191/150‑1 (MQ=255)

                                                                                                                                                cTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTaa    >  2:1901776/1‑150 (MQ=255)

                                                                                                                                                  cttCATCAGCTT‑GTGAATACGCCGCGCATTTGCCAAGGATAATTAATGGCCGTTAAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGTCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACg  >  2:1870193/2‑150 (MQ=255)

ACCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACG  >  NC_000913/2491117‑2491412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`