Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,579,157 G→T 6.6% R164R (CGG→CGT)  talA → transaldolase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,579,1570GT 6.6% 474.9 / 11.0 141R164R (CGG→CGT) talAtransaldolase A
Reads supporting (aligned to +/- strand):  ref base G (64/67);  new base T (3/7);  total (67/74)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GGACTTGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCA  >  NC_000913/2579010‑2579306
                                                                                                                                                   |                                                                                                                                                     
ggATTTGTATCAGCAACAAGGCGTTGATAAATCACGCTTTCTGATCACGCTGGATTCGACCTTGGAACGATTTGGGGGGGCAGAAGAGCTGGAATAAGAATGTATTAACTGCAACCTGACGGTGCTGTTTTCTTTTGCACAGGCACGGGc                                                                                                                                                     <  2:2104639/150‑1 (MQ=255)
ggACTTGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGc                                                                                                                                                     >  1:1227292/1‑150 (MQ=255)
 gACTTGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGcc                                                                                                                                                    <  1:1897720/150‑1 (MQ=255)
   cTTGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCtg                                                                                                                                                  >  2:2290339/1‑150 (MQ=255)
   cTTGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCtg                                                                                                                                                  >  2:24467/1‑150 (MQ=255)
     tGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCtgtg                                                                                                                                                >  1:2354525/1‑150 (MQ=255)
      gTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGc                                                                                                                                               <  1:494042/150‑1 (MQ=255)
      gTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGc                                                                                                                                               <  1:1210696/150‑1 (MQ=255)
       tATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAATGTATTAACTGCAACCTGACTCTGCTGTTTTCTTTTGCACAGGCCCGTGCCTGTGca                                                                                                                                              <  1:1744082/150‑2 (MQ=255)
        aTCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCgg                                                                                                                                             >  1:1429991/1‑150 (MQ=255)
         tCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGGAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCCCGTGCCTGTGcaaa                                                                                                                                            <  2:175876/150‑4 (MQ=255)
         tCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCCCGTGCCTGTGcaaa                                                                                                                                            <  2:1933453/150‑4 (MQ=255)
         tCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTCGCACAGGCCCGTGCCTGTGcaaa                                                                                                                                            <  2:1079908/150‑4 (MQ=255)
          cAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGaa                                                                                                                                           >  2:726596/1‑150 (MQ=255)
           aGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCCCGTGCCTGTGcaaaag                                                                                                                                          <  2:1489800/150‑6 (MQ=255)
            gCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGc                                                                                                                                         >  1:2032149/1‑150 (MQ=255)
            gCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGc                                                                                                                                         <  1:2121354/150‑1 (MQ=255)
            ccaacaaGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGc                                                                                                                                         <  2:582293/149‑1 (MQ=255)
               acaaGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAgg                                                                                                                                      >  1:1759392/1‑150 (MQ=255)
               acaaGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAgg                                                                                                                                      >  2:211082/1‑150 (MQ=255)
                   ggCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGtt                                                                                                                                  <  2:1904357/150‑1 (MQ=255)
                    gCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGttt                                                                                                                                 >  2:900766/1‑150 (MQ=255)
                        tGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCt                                                                                                                             >  1:2212290/1‑150 (MQ=255)
                           gaAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGAt                                                                                                                          >  1:2393549/1‑150 (MQ=255)
                             aaTCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGAttt                                                                                                                        >  2:1021444/1‑150 (MQ=255)
                             aaTCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGAttt                                                                                                                        <  1:2171795/150‑1 (MQ=255)
                                cACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGc                                                                                                                     >  2:697834/1‑150 (MQ=255)
                                 aCGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGcc                                                                                                                    <  1:949545/150‑1 (MQ=255)
                                   gCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGt                                                                                                                  <  1:820675/150‑1 (MQ=255)
                                     aTTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGttt                                                                                                                <  1:61192/150‑1 (MQ=255)
                                     aTTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGttt                                                                                                                >  1:1060677/1‑150 (MQ=255)
                                      ttCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGGGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTg                                                                                                               <  1:1026080/150‑1 (MQ=255)
                                      ttCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTg                                                                                                               <  1:2239018/150‑1 (MQ=255)
                                      ttCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTg                                                                                                               <  1:2330569/150‑1 (MQ=255)
                                        cTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTc                                                                                                             >  1:24975/1‑150 (MQ=255)
                                             cAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCg                                                                                                        <  2:1060677/150‑1 (MQ=255)
                                              aaGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGt                                                                                                       >  2:776503/1‑150 (MQ=255)
                                                   ggCTTCGACCTGGGAAGGAATTCGCGCGGGAGAAGAGCTGGAAAAAGTAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTa                                                                                                  <  1:2076071/150‑1 (MQ=255)
                                                            cTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTa                                                                                         <  2:265518/150‑1 (MQ=255)
                                                            cTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTa                                                                                         >  1:1902265/1‑150 (MQ=255)
                                                              gggAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATc                                                                                       >  1:1999019/1‑150 (MQ=255)
                                                               ggaaggaaTTTGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCa                                                                                      <  1:31755/150‑1 (MQ=255)
                                                               ggaaggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCa                                                                                      >  1:2403906/1‑150 (MQ=255)
                                                                 aaggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTc                                                                                                                       <  2:1841967/115‑1 (MQ=255)
                                                                 aaggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAgg                                                                                    <  2:122002/150‑1 (MQ=255)
                                                                 aaggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAgg                                                                                    <  2:442078/150‑1 (MQ=255)
                                                                 aaggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAgg                                                                                    <  1:1036151/150‑1 (MQ=255)
                                                                   ggaaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCa                                                                                  <  1:28093/150‑1 (MQ=255)
                                                                     aaTTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCAGGCGTATTTATGACTGGTATCAGGCACg                                                                                >  2:763051/1‑150 (MQ=255)
                                                                           cgcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTAGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGcc                                                                          <  2:691402/150‑1 (MQ=255)
                                                                            gcgGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCg                                                                         <  1:2290339/150‑1 (MQ=255)
                                                                               gCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATg                                                                      >  2:595834/1‑150 (MQ=255)
                                                                               gCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATg                                                                      <  2:1483548/150‑1 (MQ=255)
                                                                               gCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATg                                                                      <  1:1274258/150‑1 (MQ=255)
                                                                                   aagaGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAcc                                                                  <  1:843969/150‑1 (MQ=255)
                                                                                   aagaGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGAcc                                                                  <  1:2111637/150‑1 (MQ=255)
                                                                                       gCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGAGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTa                                                              >  2:114543/1‑150 (MQ=255)
                                                                                        cTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTAt                                                             >  1:761518/1‑150 (MQ=255)
                                                                                                    ggTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGgaagaa                                                 <  1:1940251/150‑1 (MQ=255)
                                                                                                    ggTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGgaagaa                                                 >  1:970396/1‑150 (MQ=255)
                                                                                                     gTATTAACTGCACCCTGACGCTGCTGTTTTGTTTTGCACATGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATATCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGaagaag                                                <  2:1397499/150‑1 (MQ=255)
                                                                                                       aTTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGAt                                              >  2:906690/1‑150 (MQ=255)
                                                                                                       aTTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCAAAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGAt                                              >  2:897542/1‑150 (MQ=255)
                                                                                                         tAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGGTcc                                            >  1:481636/1‑150 (MQ=255)
                                                                                                            cTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCggg                                         >  2:1314159/1‑150 (MQ=255)
                                                                                                            cTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCggg                                         >  2:2017172/1‑150 (MQ=255)
                                                                                                               cAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGt                                      >  1:775663/1‑150 (MQ=255)
                                                                                                                 aCCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGACGGGCGTATTTATGACTGGTATCAGGCAGGCAAGCCGATGGACACGTATGTGGGGGAAGAAGATCCGGGAGTTa                                    >  2:1610314/1‑150 (MQ=255)
                                                                                                                     gACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGCTCCGGGCGTTAAATc                                <  2:481636/150‑1 (MQ=255)
                                                                                                                       cGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCgg                              >  2:2433203/1‑150 (MQ=255)
                                                                                                                       cGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCgg                              >  2:1777711/1‑150 (MQ=255)
                                                                                                                        gctgctGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGt                             <  2:2032149/150‑1 (MQ=255)
                                                                                                                         ctgctgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATTACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTg                            <  1:551876/150‑1 (MQ=255)
                                                                                                                         ctgctgTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTg                            <  2:1250332/150‑1 (MQ=255)
                                                                                                                         ctcctgGTTTCTTTTGCCCAGGCACGGGGCTGTGCGGAAGCAGGCGTTTTGCGGCTTTCGCCGTTTGTCGGGCGTATTTATTACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGCATCAGATCCGGGCGTTAAATCGGTg                            <  2:2171296/147‑1 (MQ=255)
                                                                                                                              gTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCaa                       >  2:52008/1‑150 (MQ=255)
                                                                                                                              gTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCaa                       >  1:1200550/1‑150 (MQ=255)
                                                                                                                               ttttCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAat                      <  1:1431525/150‑1 (MQ=255)
                                                                                                                               ttttCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGACGTTAAATCGGTGCGCAat                      <  1:148562/150‑1 (MQ=255)
                                                                                                                                tttCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAata                     <  1:639732/150‑1 (MQ=255)
                                                                                                                                  tCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCTatgag                   <  2:1776147/150‑4 (MQ=255)
                                                                                                                                   cttccgCACAGGCCCGTGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCt                  >  2:1627429/6‑150 (MQ=255)
                                                                                                                                    ttttGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTa                 >  1:1237586/1‑150 (MQ=255)
                                                                                                                                     tttGCTCAGGTACGGGCGTGTGCGGAAGGTGGCGTTTTTCTGTTTTCGCGGTTTGTCGGGCGTTTTTATGTGTGGTATCAGGCAAGCTAGGCGATGGAGCCGTTTGTGGTGGATGATGTTGCGGGCGTTAAATCGGTGCGCATTATCTAc                <  2:2297831/150‑1 (MQ=255)
                                                                                                                                     tttGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGTGCGTTAAATCGGTGCGCAATATCTAc                <  2:1207691/150‑1 (MQ=255)
                                                                                                                                     tccgCACAGGCCCGTGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCTGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTAc                >  2:739390/4‑150 (MQ=255)
                                                                                                                                      ttGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACg               <  2:1759392/150‑1 (MQ=255)
                                                                                                                                      ttGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACg               <  2:924887/150‑1 (MQ=255)
                                                                                                                                         cacaGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGact            >  2:1030491/1‑150 (MQ=255)
                                                                                                                                         cacaGGCACGGGCCTGTGCGGAAGCAGGCGATTAACTGATATCGCCGTTTGACGGTCGTATTTATGACTGGTATCATGCACGCAAGACGATGGAACCGAATGTGGAGGAAGAAGATCCGGGCCTTAAATCGGTGCGCAATATCAACGaca            >  2:1431756/1‑149 (MQ=255)
                                                                                                                                           caGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGactac          <  2:2373218/150‑1 (MQ=255)
                                                                                                                                           caGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGactac          <  1:2116876/150‑1 (MQ=255)
                                                                                                                                             ggCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGActacta        >  2:1627137/1‑150 (MQ=255)
                                                                                                                                             ggCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGActacta        >  2:2463205/1‑150 (MQ=255)
                                                                                                                                               cACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACtata      <  1:353647/150‑1 (MQ=255)
                                                                                                                                                 ggggCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAg    <  1:1330289/149‑1 (MQ=255)
                                                                                                                                                 cGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAg    >  1:741506/1‑150 (MQ=255)
                                                                                                                                                  gggCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGc   <  2:191046/150‑1 (MQ=255)
                                                                                                                                                  gggCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGAAAGCCTATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGc   <  1:726596/150‑1 (MQ=255)
                                                                                                                                                   ggCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCa  <  2:2403906/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGACTTGTATCAGCAACAAGGCGTTGAGAAATCACGCATTCTGATCAAGCTGGCTTCGACCTGGGAAGGAATTCGCGCGGCAGAAGAGCTGGAAAAAGAAGGTATTAACTGCAACCTGACGCTGCTGTTTTCTTTTGCACAGGCACGGGCCTGTGCGGAAGCAGGCGTTTTTCTGATTTCGCCGTTTGTCGGGCGTATTTATGACTGGTATCAGGCACGCAAGCCGATGGACCCGTATGTGGTGGAAGAAGATCCGGGCGTTAAATCGGTGCGCAATATCTACGACTACTATAAGCA  >  NC_000913/2579010‑2579306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑