BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,593,188	T→C	6.2%	N195D (AAT→GAT)	ypfH ←	esterase YpfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,593,188	0	T	C	6.2%	496.3 / 15.6	162	N195D (AAT→GAT)	ypfH	esterase YpfH
Reads supporting (aligned to +/- strand): ref base T (73/79); new base C (3/7); total (76/86)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 162 total aligned reads displayed.

CACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGA  >  NC_000913/2593040‑2593335

cACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTg                                                                                                                                                    <  1:335547/150‑1 (MQ=255)

cACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTg                                                                                                                                                    <  1:470045/150‑1 (MQ=255)

cACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTg                                                                                                                                                    >  1:1980805/1‑150 (MQ=255)

  cgcgTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTc                                                                                                                                                  <  1:26332/150‑1 (MQ=255)

    cgTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGa                                                                                                                                                <  1:107982/150‑1 (MQ=255)

    cgTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGa                                                                                                                                                >  1:710428/1‑150 (MQ=255)

       cTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCg                                                                                                                                             <  2:1679039/150‑1 (MQ=255)

         tATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCa                                                                                                                                           >  2:2465810/1‑150 (MQ=255)

         tATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCa                                                                                                                                           >  2:361162/1‑150 (MQ=255)

            cAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATCGACAATCGCagta                                                                                                                                        <  2:687471/150‑4 (MQ=255)

            cAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATCGACAATCGCagta                                                                                                                                        <  1:1890971/150‑4 (MQ=255)

             aGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATCGACAATCGCagtat                                                                                                                                       <  1:1960845/150‑5 (MQ=255)

             aGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATCGACAATCGCagtat                                                                                                                                       <  1:1951194/150‑5 (MQ=255)

             aGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTAACTCCACTTAGCGCTTCTTCGAAATAATGTTTCGGAATGGTATAACTCAAGTGATCCAGGTAAAATTGCATACTGCGATCGACAATCGCagtat                                                                                                                                       <  2:2051135/150‑5 (MQ=255)

               gCCAGCTAATCTGTCTTATACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTAAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCt                                                                                                                                     >  2:1000149/1‑150 (MQ=255)

                ccAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTa                                                                                                                                    >  2:846540/1‑150 (MQ=255)

                      aaTCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAAtcct                                                                                                                              >  1:1144074/1‑150 (MQ=255)

                        tCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCAt                                                                                                                                          <  2:869509/136‑1 (MQ=255)

                        tCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAAtcctcc                                                                                                                            >  2:316446/1‑150 (MQ=255)

                          tGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCAc                                                                                                                          <  1:2016763/150‑1 (MQ=255)

                          tGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCAc                                                                                                                          <  1:1027740/150‑1 (MQ=255)

                           gTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACg                                                                                                                         <  1:2370654/150‑1 (MQ=255)

                             cTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGat                                                                                                                       <  1:1729739/150‑1 (MQ=255)

                              ttttACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGata                                                                                                                      <  2:736369/150‑1 (MQ=255)

                                ttACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATc                                                                                                                    >  1:802598/1‑150 (MQ=255)

                                   catcatTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAg                                                                                                                 >  1:931632/1‑150 (MQ=255)

                                   catcatTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAg                                                                                                                 >  1:710159/1‑150 (MQ=255)

                                   catcatTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAg                                                                                                                 >  2:1147587/1‑150 (MQ=255)

                                    atcatTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGc                                                                                                                >  1:874277/1‑150 (MQ=255)

                                     tcatTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcg                                                                                                               <  2:2074759/150‑1 (MQ=255)

                                     tcatTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcg                                                                                                               <  1:2167571/150‑1 (MQ=255)

                                     tcatTTCAATCACATCATCATCGCCAGGCTTAACGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcg                                                                                                               <  2:1466269/150‑1 (MQ=255)

                                      catTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcgt                                                                                                              <  2:1246774/150‑1 (MQ=255)

                                       atTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcgtc                                                                                                             >  1:752493/1‑150 (MQ=255)

                                          tCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcgtcacg                                                                                                          <  2:931632/150‑1 (MQ=255)

                                           cAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcgtcacgt                                                                                                         >  1:1070193/1‑150 (MQ=255)

                                            aaTCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcgtcacgtc                                                                                                        <  1:990161/150‑1 (MQ=255)

                                             aTCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGcgtcacgtca                                                                                                       <  1:1694110/150‑1 (MQ=255)

                                               cacaTCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTcacc                                                                                                     <  2:236030/150‑1 (MQ=255)

                                                acaTCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTcacca                                                                                                    >  2:223381/1‑150 (MQ=255)

                                                    catcatcGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCgg                                                                                                <  1:1564640/150‑1 (MQ=255)

                                                    catcatcGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCgg                                                                                                <  2:78847/150‑1 (MQ=255)

                                                      tcatcGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCa                                                                                              >  2:727972/1‑150 (MQ=255)

                                                        atcGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACt                                                                                            >  1:758594/1‑150 (MQ=255)

                                                        atcGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACt                                                                                            >  2:2418060/1‑150 (MQ=255)

                                                         ccGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATACCGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTg                                                                                           <  2:2329399/149‑1 (MQ=255)

                                                          cGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCCCCGGCACTGa                                                                                          >  1:2432731/1‑150 (MQ=255)

                                                          cGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGa                                                                                          >  2:1845219/1‑150 (MQ=255)

                                                           gCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAt                                                                                         >  1:1639065/1‑150 (MQ=255)

                                                           gCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAt                                                                                         <  1:2446925/150‑1 (MQ=255)

                                                           gCCAGGCTTACCGCCACTTAGCGCTTCATCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAt                                                                                         <  2:1709273/150‑1 (MQ=255)

                                                                gCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACg                                                                                    >  1:1027209/1‑150 (MQ=255)

                                                                gCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACg                                                                                    <  1:848379/150‑1 (MQ=255)

                                                                 cTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGc                                                                                   >  2:1286110/1‑150 (MQ=255)

                                                                    aCCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTc                                                                                <  1:1845219/150‑1 (MQ=255)

                                                                     ccGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTcc                                                                               <  2:1937405/150‑1 (MQ=255)

                                                                     ccGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTcc                                                                               <  1:1387590/150‑1 (MQ=255)

                                                                     ccGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTcc                                                                               <  2:1164398/150‑1 (MQ=255)

                                                                     ccGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTcc                                                                               <  2:604174/150‑1 (MQ=255)

                                                                      cGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCt                                                                              <  1:2374891/150‑1 (MQ=255)

                                                                        ccACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCGCCACCGGCACTGATTAACGCTTCCTgc                                                                            >  2:962035/1‑150 (MQ=255)

                                                                        ccACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTgc                                                                            <  1:1433873/150‑1 (MQ=255)

                                                                         cACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTgcg                                                                           <  1:2465810/150‑1 (MQ=255)

                                                                          aCTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTgcgc                                                                          >  2:2389671/1‑150 (MQ=255)

                                                                                 gcTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAAcc                                                                   <  2:752493/150‑1 (MQ=255)

                                                                                       tcgAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCCACCGCATgc                                                             >  2:1818400/1‑150 (MQ=255)

                                                                                       tcgAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATgc                                                             <  2:1959218/150‑1 (MQ=255)

                                                                                       tcgAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATgc                                                             >  2:1935471/1‑150 (MQ=255)

                                                                                        cgAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATgcg                                                            <  1:796613/150‑1 (MQ=255)

                                                                                          aaaTAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCGTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAAGCGCATGCGcc                                                          <  2:111689/150‑1 (MQ=255)

                                                                                          aaaTAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGcc                                                          <  1:639214/150‑1 (MQ=255)

                                                                                           aataatGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCa                                                         >  1:965483/1‑150 (MQ=255)

                                                                                                    tCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCCCGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATaa                                                >  2:2067948/1‑150 (MQ=255)

                                                                                                         aTGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGa                                           >  2:1431957/1‑150 (MQ=255)

                                                                                                           ggTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATc                                         >  2:831954/1‑150 (MQ=255)

                                                                                                           ggTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATc                                         <  1:2286255/150‑1 (MQ=255)

                                                                                                            gTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCt                                        >  1:1588549/1‑150 (MQ=255)

                                                                                                                  cGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCg                                  <  1:2005882/150‑1 (MQ=255)

                                                                                                                  cGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCg                                  >  2:1466558/1‑150 (MQ=255)

                                                                                                                      aGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCAt                              <  2:1017407/150‑1 (MQ=255)

                                                                                                                      aGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCAt                              >  1:89661/1‑150 (MQ=255)

                                                                                                                         taTCCAGGGCAAATTGCATACTGCTATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGACAGATCTATAACCGGATCTTCACCGCCATGaa                           <  2:2121735/149‑1 (MQ=255)

                                                                                                                           tCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATc                         >  2:1556485/1‑150 (MQ=255)

                                                                                                                              aGGGCAAATTGCATACTGCGATTGTCGATAGCATGGCCTAAATCCTCCACGATTTCCAGCGTCACGTCACCACCGGCACTGATTAACTCTTCCTGCGCTTATTTTTTATGCTTAAGTTGTATAACGTGATCTTCACCGCCATGAATCAgg                      <  2:2257444/150‑1 (MQ=255)

                                                                                                                                ggCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATTCTCCAGATCTATAACCTGATCTTCACCGCCATGAATCAGGTg                    <  2:802598/150‑1 (MQ=255)

                                                                                                                                  cAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGaa                  >  1:721171/1‑150 (MQ=255)

                                                                                                                                  cAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGaa                  >  2:1784156/1‑150 (MQ=255)

                                                                                                                                       tGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTc             >  2:2222538/1‑150 (MQ=255)

                                                                                                                                       cacgttcTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTc             <  2:277393/144‑1 (MQ=255)

                                                                                                                                        gCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCg            >  2:2002657/1‑150 (MQ=255)

                                                                                                                                         ggccatGCGATCGACAATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGt           >  1:334194/6‑150 (MQ=255)

                                                                                                                                          gccatGCGATCGACAATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTg          >  1:1201913/5‑150 (MQ=255)

                                                                                                                                           tACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTgg         <  1:361162/150‑1 (MQ=255)

                                                                                                                                           ccatGCGATCGACAATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTgg         >  1:228984/4‑150 (MQ=255)

                                                                                                                                            aCTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGc        <  1:2139154/150‑1 (MQ=255)

                                                                                                                                             cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCt       >  1:22348/1‑150 (MQ=255)

                                                                                                                                             cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCt       >  2:94003/1‑150 (MQ=255)

                                                                                                                                               gCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGt     >  1:1346232/1‑150 (MQ=255)

                                                                                                                                                cGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTa    >  1:148581/1‑150 (MQ=255)

                                                                                                                                                  aTTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGa  <  1:1294565/150‑1 (MQ=255)

CACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGA  >  NC_000913/2593040‑2593335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`