BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,624,195	C→G	6.8%	D384E (GAC→GAG)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,195	0	C	G	6.8%	528.5 / 21.7	163	D384E (GAC→GAG)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand): ref base C (77/74); new base G (5/6); total (83/80)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 168 total aligned reads displayed.

GCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAAT  >  NC_000913/2624046‑2624344

   aTTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCg                                                                                                                                                    >  1:2159273/1‑150 (MQ=255)

       gcgAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCgatga                                                                                                                                                >  1:2367418/1‑150 (MQ=255)

        cgAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCgatgat                                                                                                                                               <  1:698218/150‑1 (MQ=255)

         gAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGTCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATc                                                                                                                                              >  2:805430/1‑150 (MQ=255)

           aCGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGAGTCgatgatgcg                                                                                                                                            <  1:796297/150‑4 (MQ=255)

            cgcgATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGAGTCgatgatgcgt                                                                                                                                           <  1:660611/150‑5 (MQ=255)

             gcgATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCTATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGAGTCgatgatacgtg                                                                                                                                          <  2:740205/150‑6 (MQ=255)

               gATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAcgcc                                                                                                                                        <  2:1643856/150‑1 (MQ=255)

               gATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAcgcc                                                                                                                                        <  2:2204233/150‑1 (MQ=255)

                   tgtTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGcac                                                                                                                                    >  2:1255856/1‑150 (MQ=255)

                      tGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATa                                                                                                                                 >  1:336940/1‑150 (MQ=255)

                          ctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACgg                                                                                                                             >  2:966582/1‑150 (MQ=255)

                          ctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACgg                                                                                                                             <  1:929554/150‑1 (MQ=255)

                            attatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTa                                                                                                                           >  1:1330335/1‑150 (MQ=255)

                                tCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaa                                                                                                                       <  1:818481/150‑1 (MQ=255)

                                    tATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTc                                                                                                                   >  1:292036/1‑150 (MQ=255)

                                      tCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc                                                                                                                 >  1:1576425/1‑150 (MQ=255)

                                      tCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCAACGCCGCACATAACGGTAAGAAAGTCaa                                                                                                                 >  2:558303/1‑149 (MQ=255)

                                       cacaCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAcc                                                                                                                >  2:1715623/1‑150 (MQ=255)

                                           ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg                                                                                                            <  2:761268/150‑1 (MQ=255)

                                           ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg                                                                                                            >  2:1132327/1‑150 (MQ=255)

                                            cTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                                                           <  1:1081121/150‑1 (MQ=255)

                                               tGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTggtggt                                                                                                        >  2:2334247/1‑150 (MQ=255)

                                                 aGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTg                                                                                                      >  1:865097/1‑150 (MQ=255)

                                                   cATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg                                                                                                    <  1:109898/150‑1 (MQ=255)

                                                   cATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg                                                                                                    <  1:1756486/150‑1 (MQ=255)

                                                    aTGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGt                                                                                                   >  1:488017/1‑150 (MQ=255)

                                                       tgCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTAc                                                                                                >  1:209509/1‑150 (MQ=255)

                                                       tgCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGT‑GCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa                                                                                               <  1:2301033/150‑1 (MQ=255)

                                                        gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa                                                                                               <  1:80935/150‑1 (MQ=255)

                                                        gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa                                                                                               >  1:37835/1‑150 (MQ=255)

                                                         cTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAg                                                                                              <  1:1907424/150‑1 (MQ=255)

                                                           ggAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgc                                                                                            >  2:840935/1‑150 (MQ=255)

                                                           ggAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgc                                                                                            >  2:670442/1‑150 (MQ=255)

                                                            gAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcg                                                                                           <  2:850167/150‑1 (MQ=255)

                                                              aCTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGcgcg                                                                                         <  1:2334247/150‑1 (MQ=255)

                                                              aCTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGcgcg                                                                                         <  2:2446847/150‑1 (MQ=255)

                                                                  ctgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTc                                                                                     <  1:437110/150‑1 (MQ=255)

                                                                  ctgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTc                                                                                     <  2:793043/150‑1 (MQ=255)

                                                                         atgCTTCGTTCGACAAGAGAATACTTGACATAAAAATTAACAATTACAGCTAGCCGAAACATTAAAACATAATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACgaag                                                                              >  2:517394/3‑150 (MQ=255)

                                                                          ggCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACgaaga                                                                             >  2:1011915/1‑150 (MQ=255)

                                                                            cTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGaagaag                                                                           >  2:1728709/1‑150 (MQ=255)

                                                                            cTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGaagaag                                                                           <  1:2389005/150‑1 (MQ=255)

                                                                             ttcgttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCGCGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGc                                                                          >  1:189287/1‑150 (MQ=255)

                                                                             ttcgttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGc                                                                          >  1:399151/1‑150 (MQ=255)

                                                                             ttcgttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGc                                                                          >  2:1392474/1‑150 (MQ=255)

                                                                                gttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa                                                                                               <  1:365177/126‑1 (MQ=255)

                                                                                gttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa                                                                                               >  2:365177/1‑126 (MQ=255)

                                                                                gttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCaa                                                                       >  1:368712/1‑150 (MQ=255)

                                                                                 ttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAAc                                                                      <  1:542004/150‑1 (MQ=255)

                                                                                 ttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAAc                                                                      <  1:2352472/150‑1 (MQ=255)

                                                                                   cgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCTTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACAt                                                                    <  1:857851/150‑1 (MQ=255)

                                                                                     aCCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTc                                                                  >  1:22274/1‑150 (MQ=255)

                                                                                      cccGAGCCTACTGGAGATTAAAATTAACATATACCGCGTGGAGAAAGATTCACGCATAATCTAATAGATAATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa                                                                 >  2:1436891/1‑150 (MQ=255)

                                                                                       ccGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCAc                                                                <  2:405784/150‑1 (MQ=255)

                                                                                        cGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACt                                                               >  2:309540/1‑150 (MQ=255)

                                                                                          aGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTgg                                                             <  1:706692/150‑1 (MQ=255)

                                                                                           gCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTggg                                                            >  1:1484362/1‑150 (MQ=255)

                                                                                            cGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGc                                                           <  1:767388/150‑1 (MQ=255)

                                                                                             gTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAATGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCg                                                          <  1:1119579/150‑1 (MQ=255)

                                                                                             gTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCg                                                          <  1:6008/150‑1 (MQ=255)

                                                                                             gTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCg                                                          <  2:1576425/150‑1 (MQ=255)

                                                                                                 tGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGcac                                                                                                                                    <  2:520188/72‑1 (MQ=255)

                                                                                                  ggCGATTAAAATTAACATTTACCGCGTGGCGAAAGAGTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAgcg                                                     >  2:665736/1‑150 (MQ=255)

                                                                                                   gCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAgcgc                                                    <  1:1178859/150‑1 (MQ=255)

                                                                                                    cGATTATAATTCACATTTACCGCGTGGGGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGTCGCGGTTCGACGACGAAGCCAACATTGACTGGGCGAAGCGcc                                                   <  2:835539/150‑1 (MQ=255)

                                                                                                    cGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGcc                                                   <  1:2236595/150‑1 (MQ=255)

                                                                                                           aaTTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGa                                            <  1:840935/150‑1 (MQ=255)

                                                                                                            aTTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGaa                                           >  2:311429/1‑150 (MQ=255)

                                                                                                               aaCATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCa                                        <  2:2283554/150‑1 (MQ=255)

                                                                                                                 cATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAATAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAgg                                      <  2:469294/150‑1 (MQ=255)

                                                                                                                 cATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAgg                                      >  2:1229975/1‑150 (MQ=255)

                                                                                                                 cATTTACCGCGGGGCGAAAGCTTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGACAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCCACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAgg                                      <  1:1617398/150‑1 (MQ=255)

                                                                                                                   tttACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCg                                    >  1:1291903/1‑150 (MQ=255)

                                                                                                                           gTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGtt                            >  1:2175526/1‑150 (MQ=255)

                                                                                                                            tGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTa                           <  2:399151/150‑1 (MQ=255)

                                                                                                                              gCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACGGTGGTGGGTGAGTTACAGGCGCGTTTCGGCGAAGCAGCCGACATTCGCTGGGCGAGGCGGCTGACCGAAGCAGGGGTGCGCGtggtg                         >  2:2353428/1‑146 (MQ=255)

                                                                                                                               cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtct                        <  1:1237089/150‑1 (MQ=255)

                                                                                                                               cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtct                        <  1:1322042/150‑1 (MQ=255)

                                                                                                                               cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtct                        <  1:2410962/150‑1 (MQ=255)

                                                                                                                               cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtct                        >  1:1264007/1‑150 (MQ=255)

                                                                                                                                gAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtctt                       >  1:1448782/1‑150 (MQ=255)

                                                                                                                                 aaaGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtcttc                      <  1:2079940/150‑1 (MQ=255)

                                                                                                                                     aTTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTg                  <  2:1625686/150‑1 (MQ=255)

                                                                                                                                     aTTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTg                  <  1:2471538/150‑1 (MQ=255)

                                                                                                                                       gcgtggatcatcGAGTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTgcg                >  1:2287768/7‑150 (MQ=255)

                                                                                                                                         gtggatcatcGAGTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtc                         <  1:1495644/135‑1 (MQ=255)

                                                                                                                                         gtggatcatcGAGTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGcc              >  2:1669484/5‑150 (MQ=255)

                                                                                                                                         gtggatcatcGAGTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGcc              >  1:1386881/5‑150 (MQ=255)

                                                                                                                                            catcatCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCggg           <  1:634552/150‑1 (MQ=255)

                                                                                                                                            catcatCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCggg           >  2:1109572/1‑150 (MQ=255)

                                                                                                                                               catcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCt        <  2:1291903/150‑1 (MQ=255)

                                                                                                                                                atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTg       >  2:2170665/1‑150 (MQ=255)

                                                                                                                                                 tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGa      >  1:2327009/1‑150 (MQ=255)

                                                                                                                                                   gACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGaaa    >  1:1085450/1‑150 (MQ=255)

                                                                                                                                                    aCTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGaaaa   >  1:326267/1‑150 (MQ=255)

                                                                                                                                                    aCTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGaaaa   <  1:1809450/150‑1 (MQ=255)

                                                                                                                                                     cTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAAt  >  2:1884417/1‑150 (MQ=255)

                                                                                                                                                     cTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAAt  >  2:2041872/1‑150 (MQ=255)

                                                                                                                                                     cTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAAt  >  1:1971794/1‑150 (MQ=255)

GCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAAT  >  NC_000913/2624046‑2624344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`