BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,445	T→A	5.2%	E20V (GAG→GTG)	yqaB ←	fructose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,445	0	T	A	5.2%	532.8 / 9.6	166	E20V (GAG→GTG)	yqaB	fructose‑1‑phosphatase
Reads supporting (aligned to +/- strand): ref base T (80/77); new base A (4/5); total (84/82)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGA  >  NC_000913/2817296‑2817591

cATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCt                                                                                                                                                    >  2:2095080/1‑150 (MQ=255)

 aTGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTc                                                                                                                                                   <  2:2444712/150‑1 (MQ=255)

   gCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCg                                                                                                                                                 <  2:1851953/150‑1 (MQ=255)

    cGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGt                                                                                                                                                >  1:1798201/1‑150 (MQ=255)

     gggTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTa                                                                                                                                               <  2:114634/150‑1 (MQ=255)

     gggTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTa                                                                                                                                               <  1:1926014/150‑1 (MQ=255)

      ggTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTAt                                                                                                                                              <  1:456919/150‑1 (MQ=255)

      ggTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTAt                                                                                                                                              >  2:352892/1‑150 (MQ=255)

       gTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATc                                                                                                                                             <  2:294409/150‑1 (MQ=255)

        tCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATcc                                                                                                                                            <  1:2018541/150‑1 (MQ=255)

        tCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGAGCCTACGCACCGTaaag                                                                                                                                            <  2:2174039/150‑4 (MQ=255)

        tCGAGATAGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCGTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGAGCCTACGCACCGTaaag                                                                                                                                            <  2:633572/150‑4 (MQ=255)

         cGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGAGCCTACGCACCGTaaagc                                                                                                                                           <  1:907260/150‑5 (MQ=255)

          tagaTCGGCGTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGTCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGAGCCTACGCACCGTaaagcg                                                                                                                                          <  2:250454/149‑6 (MQ=255)

           agaTCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAgg                                                                                                                                         <  1:256728/150‑1 (MQ=255)

            gaTCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGa                                                                                                                                        <  2:1596283/150‑1 (MQ=255)

                 gCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGCCCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATtgtg                                                                                                                                   >  2:1958335/1‑150 (MQ=255)

                  ccTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGc                                                                                                                                  <  2:929669/150‑1 (MQ=255)

                     gATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGccat                                                                                                                               >  1:1150232/1‑150 (MQ=255)

                     gATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGccat                                                                                                                               <  1:1543792/150‑1 (MQ=255)

                        tCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGccatcca                                                                                                                            <  1:383336/150‑1 (MQ=255)

                            cTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATc                                                                                                                        >  1:1753215/1‑150 (MQ=255)

                            cTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGCGCCATCCATATc                                                                                                                        <  1:260223/150‑1 (MQ=255)

                              cAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCaa                                                                                                                      >  2:1487556/1‑150 (MQ=255)

                                ataatTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCaaaa                                                                                                                    >  2:1187772/1‑150 (MQ=255)

                                      gCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTaaa                                                                                                              >  1:1557200/1‑150 (MQ=255)

                                       ccTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAAc                                                                                                             <  1:1573672/150‑1 (MQ=255)

                                        cTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAAcc                                                                                                            >  2:716348/1‑150 (MQ=255)

                                         tGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCACCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCt                                                                                                           >  1:810462/1‑150 (MQ=255)

                                          gAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTg                                                                                                          <  1:1623889/150‑1 (MQ=255)

                                           aGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGc                                                                                                         <  2:2000904/150‑1 (MQ=255)

                                            gCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCa                                                                                                        <  1:869685/150‑1 (MQ=255)

                                               aTACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATaa                                                                                                     >  2:1630416/1‑150 (MQ=255)

                                                  cGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGc                                                                                                  >  1:2248595/1‑150 (MQ=255)

                                                  cGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGc                                                                                                  >  1:1398000/1‑150 (MQ=255)

                                                     cAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCg                                                                                               <  1:101503/150‑1 (MQ=255)

                                                      aGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGt                                                                                              >  2:843506/1‑150 (MQ=255)

                                                          gggCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACAt                                                                                          <  2:1357229/150‑1 (MQ=255)

                                                            gCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATgg                                                                                        >  2:268513/1‑150 (MQ=255)

                                                            gCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATgg                                                                                        >  2:716181/1‑150 (MQ=255)

                                                             cGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGt                                                                                       >  2:2190613/1‑150 (MQ=255)

                                                              gATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTa                                                                                                                                               >  1:1853268/1‑93 (MQ=255)

                                                               aTCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGtct                                                                                     >  2:1404701/1‑150 (MQ=255)

                                                                 ccATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGtcttc                                                                                   >  2:2290173/1‑150 (MQ=255)

                                                                 ccATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAACACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGtcttc                                                                                   <  2:415020/150‑1 (MQ=255)

                                                                  cATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGtcttct                                                                                  <  1:2205036/150‑1 (MQ=255)

                                                                  cATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGtcttct                                                                                  >  2:2338437/1‑150 (MQ=255)

                                                                  cATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGtcttct                                                                                  >  2:1044364/1‑150 (MQ=255)

                                                                     tAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGc                                                                               <  2:1959455/150‑1 (MQ=255)

                                                                        gcgcAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAg                                                                            >  2:10992/1‑150 (MQ=255)

                                                                          gcAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTc                                                                          >  1:2372712/1‑150 (MQ=255)

                                                                            aaTCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTACATAACGCTCGTACATGGTCTTCTCGCAAGTCGa                                                                        >  2:537628/1‑150 (MQ=255)

                                                                             ttcataGCCTGATCATCGTACTGATGACCTTTGTGCACTAATACTTCGCGCCAAGCTTTACGTTGCGTAGGCTCGGTATCCAGGATGGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTAGaa                                                                       <  1:1057771/149‑1 (MQ=255)

                                                                              tcatcGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGaaa                                                                      <  1:2095080/150‑1 (MQ=255)

                                                                               catcGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGaaaa                                                                     <  1:2449346/150‑1 (MQ=255)

                                                                                  cGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAata                                                                  >  1:1154825/1‑150 (MQ=255)

                                                                                    ccTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATg                                                                >  2:132051/1‑150 (MQ=255)

                                                                                    ccTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATg                                                                <  2:2396087/150‑1 (MQ=255)

                                                                                      tGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGAc                                                              <  1:1106026/150‑1 (MQ=255)

                                                                                      tGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGAc                                                              >  1:860203/1‑150 (MQ=255)

                                                                                           atCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTAc                                                         <  1:2000386/150‑1 (MQ=255)

                                                                                                  tGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCg                                                  <  2:2248595/150‑1 (MQ=255)

                                                                                                   gAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGt                                                 >  1:1358573/1‑150 (MQ=255)

                                                                                                   gAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGt                                                 <  1:1630416/150‑1 (MQ=255)

                                                                                                       aCCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAAtt                                             >  1:917586/1‑150 (MQ=255)

                                                                                                        ccGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTg                                            <  1:2290173/150‑1 (MQ=255)

                                                                                                           tAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCtt                                         <  2:103887/150‑1 (MQ=255)

                                                                                                           tAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCtt                                         >  2:213529/1‑150 (MQ=255)

                                                                                                               gCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGAtt                                     <  2:2388336/150‑1 (MQ=255)

                                                                                                               gCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGAtt                                     >  2:2368651/1‑150 (MQ=255)

                                                                                                                cccTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGAttt                                    >  1:1733908/1‑150 (MQ=255)

                                                                                                                  cTAATACTTCGCGCCACGCTTTAAGTTTCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAAACTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATTACGCTACTATAGCGTAATTGCTTTAttttt                                  >  2:2128767/1‑149 (MQ=255)

                                                                                                                   tAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGt                                 >  1:712323/1‑150 (MQ=255)

                                                                                                                   tAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGt                                 >  2:2182083/1‑150 (MQ=255)

                                                                                                                     aTACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCg                               >  1:467243/1‑150 (MQ=255)

                                                                                                                        cTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTg                            <  1:481827/150‑1 (MQ=255)

                                                                                                                           cgcgCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATg                         <  2:1649747/150‑1 (MQ=255)

                                                                                                                           cgcgCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATg                         <  2:549610/150‑1 (MQ=255)

                                                                                                                            gcgcCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATgg                        <  1:273575/150‑1 (MQ=255)

                                                                                                                              gcCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCg                      <  2:1557200/150‑1 (MQ=255)

                                                                                                                               ccACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGa                     <  1:1296849/150‑1 (MQ=255)

                                                                                                                               ccACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGa                     >  2:1801644/1‑150 (MQ=255)

                                                                                                                               ccACGCTTTACGGTACGTAGGCTCCGTATCCAGGATTCTGACATCCAAATCAAAAATTAAACCTGAATAACGCTCGTAAATAGTATTATCGCAAGACGAAAAATAAGACGCTACTTTAGCGTAATTGCTTGATTATGTCGCTAATGGCGa                     >  2:1873719/1‑150 (MQ=255)

                                                                                                                                 aCGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAg                   <  1:209623/150‑1 (MQ=255)

                                                                                                                                  ctggatACGGAGCCTACGCACTGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAgg                  >  2:1840292/6‑150 (MQ=255)

                                                                                                                                  cGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAgg                  >  2:1450636/1‑150 (MQ=255)

                                                                                                                                  cGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAgg                  <  1:1661141/150‑1 (MQ=255)

                                                                                                                                   gCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGga                 >  1:1452394/1‑150 (MQ=255)

                                                                                                                                   gCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGga                 >  1:2363646/1‑150 (MQ=255)

                                                                                                                                    cTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGgag                <  1:1803182/150‑1 (MQ=255)

                                                                                                                                        aCGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGagaaga            >  1:1694137/1‑150 (MQ=255)

                                                                                                                                         cGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACTCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAgaagaa           >  1:2134830/1‑150 (MQ=255)

                                                                                                                                         cGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAgaagaa           <  1:582874/150‑1 (MQ=255)

                                                                                                                                         cGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAgaagaa           <  1:716181/150‑1 (MQ=255)

                                                                                                                                              cGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTc      <  2:163613/150‑1 (MQ=255)

                                                                                                                                              cGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTc      <  2:1785893/150‑1 (MQ=255)

                                                                                                                                               gTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCa     >  1:1939634/1‑150 (MQ=255)

                                                                                                                                                tAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAg    >  2:310807/1‑150 (MQ=255)

                                                                                                                                                tAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAg    <  1:352892/150‑1 (MQ=255)

                                                                                                                                                  ggCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGa  >  2:1754218/1‑150 (MQ=255)

CATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGTAGGCTCCGTATCCAGGATTGTGCCATCCATATCAAAAATTAAACCTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGA  >  NC_000913/2817296‑2817591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`