BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,906,608	G→C	6.1%	intergenic (+25/+35)	ygcG → / ← eno	protein YgcG/enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,608	0	G	C	6.1%	614.4 / 21.0	181	intergenic (+25/+35)	ygcG/eno	protein YgcG/enolase
Reads supporting (aligned to +/- strand): ref base G (81/89); new base C (6/5); total (87/94)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

Only 100 of 182 total aligned reads displayed.

AGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGA  >  NC_000913/2906459‑2906756

aGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCg                                                                                                                                                      <  1:2260899/150‑1 (MQ=255)

       agCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTGGAGGCTg                                                                                                                                               <  2:1716468/150‑1 (MQ=255)

         cATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGc                                                                                                                                             <  1:298265/150‑1 (MQ=255)

          aacTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGACGCTCAGATAGCGGCGGATATAGCGGCGGGCGCGGTTCTTCTGCAGGTGGCGGCGCATCCGGCCGCTGGTAACTAAACCATAAAAAAAGCCAGCCCGGAggcaggca                                                                                                                                            >  2:2195640/3‑150 (MQ=255)

           tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCTGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAt                                                                                                                                           <  2:328440/150‑1 (MQ=255)

           tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAt                                                                                                                                           >  1:410130/1‑150 (MQ=255)

            cTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtt                                                                                                                                          >  2:1247509/1‑150 (MQ=255)

              ggTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtttt                                                                                                                                        >  2:2007230/1‑150 (MQ=255)

              ggTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtttt                                                                                                                                        >  2:2115557/1‑150 (MQ=255)

               gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAttttt                                                                                                                                       <  1:2384137/150‑1 (MQ=255)

               gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttttt                                                                                                                                       <  2:819304/150‑1 (MQ=255)

                tAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGTCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTa                                                                                                                                      <  2:1887163/150‑1 (MQ=255)

                  gAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAttttttat                                                                                                                                    <  1:233127/150‑4 (MQ=255)

                      ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAg                                                                                                                                <  1:759524/150‑1 (MQ=255)

                       tATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGa                                                                                                                               <  2:1560962/150‑1 (MQ=255)

                        aTCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGAt                                                                                                                              >  2:1059432/1‑150 (MQ=255)

                         tCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATa                                                                                                                             >  2:1373185/1‑150 (MQ=255)

                          cATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaa                                                                                                                            >  1:528107/1‑150 (MQ=255)

                          cATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaa                                                                                                                            >  1:1310050/1‑150 (MQ=255)

                           aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaaa                                                                                                                           >  2:438115/1‑150 (MQ=255)

                            ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAag                                                                                                                          <  1:620450/150‑1 (MQ=255)

                                gACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcag                                                                                                                      >  1:1969128/1‑150 (MQ=255)

                                  caacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTCGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcagtc                                                                                                                    >  2:650636/1‑150 (MQ=255)

                                    acaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCa                                                                                                                                            >  2:1138591/1‑124 (MQ=255)

                                    acaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCa                                                                                                                                            <  1:1138591/124‑1 (MQ=255)

                                      aGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTAt                                                                                                                >  1:343552/1‑150 (MQ=255)

                                       gCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATg                                                                                                               >  2:259962/1‑150 (MQ=255)

                                         ggTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGcc                                                                                                             <  2:312681/150‑1 (MQ=255)

                                          gTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCt                                                                                                            >  2:1633009/1‑150 (MQ=255)

                                            tCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTgg                                                                                                          <  2:1579892/150‑1 (MQ=255)

                                              cGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGcc                                                                                                        >  2:1270235/1‑150 (MQ=255)

                                                  ggAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTg                                                                                                    >  2:1216304/1‑150 (MQ=255)

                                                   gAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTATGGAGGGGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGa                                                                                                   <  2:1474174/150‑1 (MQ=255)

                                                     ggCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGacc                                                                                                 <  1:1260313/150‑3 (MQ=255)

                                                       cTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAtctc                                                                                               >  2:927623/1‑150 (MQ=255)

                                                          aGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCttt                                                                                            >  1:1336637/1‑150 (MQ=255)

                                                            aTAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTTGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTAc                                                                                          <  2:37480/150‑1 (MQ=255)

                                                               gcggcgGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGAc                                                                                       <  1:1902995/150‑1 (MQ=255)

                                                                 ggcggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCg                                                                                     >  2:2474535/1‑150 (MQ=255)

                                                                 ggcggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCg                                                                                     <  2:2011496/150‑1 (MQ=255)

                                                                  gcggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGt                                                                                    <  1:707281/150‑1 (MQ=255)

                                                                    ggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTg                                                                                  <  1:1187608/150‑1 (MQ=255)

                                                                            cggcggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGc                                                                          <  1:1247509/150‑1 (MQ=255)

                                                                            cggcggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGc                                                                          <  1:1068695/150‑1 (MQ=255)

                                                                              gcggGGGCGGTTCTTCTGGAGGTGGCGGCGTATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCtt                                                                        >  2:1849398/1‑150 (MQ=255)

                                                                                gggggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCtttt                                                                      <  2:576247/150‑1 (MQ=255)

                                                                                 ggggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCttttt                                                                     >  1:908077/1‑150 (MQ=255)

                                                                                  gggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTc                                                                    >  2:2111914/1‑150 (MQ=255)

                                                                                  gggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTc                                                                    <  1:1059432/150‑1 (MQ=255)

                                                                                   ggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCg                                                                   <  1:2007230/150‑1 (MQ=255)

                                                                                   ggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCg                                                                   <  2:1310050/150‑1 (MQ=255)

                                                                                     cGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGcc                                                                 <  1:1759209/150‑1 (MQ=255)

                                                                                      ggTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGccc                                                                <  1:226474/150‑1 (MQ=255)

                                                                                      ggTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGccc                                                                >  2:1025383/1‑150 (MQ=255)

                                                                                       gTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCa                                                               >  2:445150/1‑150 (MQ=255)

                                                                                          cttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCagag                                                            <  2:1694219/150‑1 (MQ=255)

                                                                                                gAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGcttctt                                                      <  2:841367/150‑1 (MQ=255)

                                                                                                 aGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCttcttc                                                     <  1:1435172/150‑1 (MQ=255)

                                                                                                   gTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGa                                                   >  2:1077610/1‑150 (MQ=255)

                                                                                                    tGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGGCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGAt                                                  >  2:555197/1‑150 (MQ=255)

                                                                                                    tGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGAt                                                  >  1:2067892/1‑150 (MQ=255)

                                                                                                     ggcggcGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATa                                                 >  1:319215/1‑150 (MQ=255)

                                                                                                     ggcggcGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATa                                                 >  2:251249/1‑150 (MQ=255)

                                                                                                      gcggcgAATCCGGCCGCTGGTAACTCAACCATAAAAAATGGCAGCCCGGAGTCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTTATCTCTTTACGACAGTTGTACGTTGCTTTTTCGCCGATAGCTTCTTCGATAc                                                <  2:1121003/150‑1 (MQ=255)

                                                                                                        ggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGGTACGa                                              >  1:54770/1‑150 (MQ=255)

                                                                                                         gcgcATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGaa                                             <  1:18287/150‑1 (MQ=255)

                                                                                                            cATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCa                                          >  2:628710/1‑150 (MQ=255)

                                                                                                            cATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCa                                          >  2:1965559/1‑150 (MQ=255)

                                                                                                                cGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTg                                      >  1:1283138/1‑150 (MQ=255)

                                                                                                                 ggCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTgg                                     <  2:1340366/150‑1 (MQ=255)

                                                                                                                           aaCTAAACCATAAAAAATGCCTGCCGGTCGGTTGGAATTTTAAATTCTGTTAATGTCCGT‑GTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGc                          <  2:477570/150‑1 (MQ=255)

                                                                                                                            aCTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATGAGATAAAGTCCGTCTTATGCCTTGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTatc                          <  2:515467/150‑3 (MQ=255)

                                                                                                                            aCTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATATAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGc                          <  1:1690034/150‑1 (MQ=255)

                                                                                                                            aCTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGc                          >  1:1801494/1‑150 (MQ=255)

                                                                                                                             cTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTGTGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCa                         >  2:1401736/1‑150 (MQ=255)

                                                                                                                             cTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGt                                    >  1:256663/1‑139 (MQ=255)

                                                                                                                             cTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCa                         >  2:1789005/1‑150 (MQ=255)

                                                                                                                             cTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCa                         >  1:242963/1‑150 (MQ=255)

                                                                                                                                aaCCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAaca                      <  1:927623/150‑1 (MQ=255)

                                                                                                                                 aCCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAacac                     >  1:325796/1‑150 (MQ=255)

                                                                                                                                 aCCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAacac                     <  1:2040227/150‑1 (MQ=255)

                                                                                                                                   cATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACgg                   >  1:1522446/1‑150 (MQ=255)

                                                                                                                                    tttaaaaaTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt                  >  1:970360/4‑150 (MQ=255)

                                                                                                                                    aTAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt                  >  1:1641279/1‑150 (MQ=255)

                                                                                                                                     tAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTc                 <  2:343552/150‑1 (MQ=255)

                                                                                                                                       aaaaaTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAg               >  2:2313850/1‑150 (MQ=255)

                                                                                                                                       aaaaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAg               >  2:2229701/1‑150 (MQ=255)

                                                                                                                                       aaaaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAg               >  1:102300/1‑150 (MQ=255)

                                                                                                                                       aaaaaTGCCAGCCCGGAGGATGGCATTTTTAAATCAGATAAAGTCAGTCTTATGACTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGATGGTTGTATTTAGCAACACGGTCAg               >  1:2227067/1‑150 (MQ=255)

                                                                                                                                        aaaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGa              >  2:172009/1‑150 (MQ=255)

                                                                                                                                          aaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAAc            <  2:2067892/150‑1 (MQ=255)

                                                                                                                                           aTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACg           >  2:671316/1‑150 (MQ=255)

                                                                                                                                           aTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACg           <  1:438115/150‑1 (MQ=255)

                                                                                                                                             gCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGc         <  1:1726597/150‑1 (MQ=255)

                                                                                                                                             gCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTGTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGACCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACgcc         >  1:1959676/1‑148 (MQ=255)

                                                                                                                                              ccAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCt        >  2:2127373/1‑150 (MQ=255)

                                                                                                                                                 gCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCAt     <  1:2115557/150‑1 (MQ=255)

                                                                                                                                                 gCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCAt     >  2:1093985/1‑150 (MQ=255)

                                                                                                                                                  cccGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATa    <  2:1275532/150‑1 (MQ=255)

                                                                                                                                                   ccGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAg   <  1:2195640/150‑1 (MQ=255)

AGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGA  >  NC_000913/2906459‑2906756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`