Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,215,997 G→C 6.7% A165A (GCC→GCG)  nfeF ← NADPH‑dependent ferric chelate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,9970GC 6.7% 418.3 / 10.8 125A165A (GCC→GCG) nfeFNADPH‑dependent ferric chelate reductase
Reads supporting (aligned to +/- strand):  ref base G (68/47);  new base C (3/6);  total (72/53)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCAT  >  NC_000913/3215848‑3216146
                                                                                                                                                     |                                                                                                                                                     
tcGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACg                                                                                                                                                       <  2:1254934/150‑1 (MQ=255)
tcGGCAGGGATTTGCATCTCCGCCAGACGCGCATCTACCGCCTGCGCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACg                                                                                                                                                       <  2:1246363/150‑1 (MQ=255)
 cGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACgg                                                                                                                                                      <  1:953268/150‑1 (MQ=255)
 cGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACgg                                                                                                                                                      <  2:203926/150‑1 (MQ=255)
        gATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGttt                                                                                                                                               <  1:141696/150‑1 (MQ=255)
          tttGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGc                                                                                                                                             >  2:1388169/1‑150 (MQ=255)
           ttGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCt                                                                                                                                            <  2:621231/150‑1 (MQ=255)
           ttGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGTTAAACCGCAAGttagtg                                                                                                                                            <  1:1561489/150‑5 (MQ=255)
           ttGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCCGTTAAACCGCAAGttagtg                                                                                                                                            <  2:1022376/150‑5 (MQ=255)
             gCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCa                                                                                                                                          >  1:651850/1‑150 (MQ=255)
              cATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCaa                                                                                                                                         >  1:1051304/1‑150 (MQ=255)
                   gcgcCAGACGCGCATCTACCGCCTGCTCATCATGTGCAAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGttt                                                                                                                                    >  2:2093732/1‑150 (MQ=255)
                    cgcCAGCCGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTGTCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTc                                                                                                                                   >  1:1604122/1‑150 (MQ=255)
                     gcCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTcc                                                                                                                                  >  2:1559522/1‑150 (MQ=255)
                      ccAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCa                                                                                                                                 <  2:1013234/150‑1 (MQ=255)
                            gcgcATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAggcggc                                                                                                                           <  2:2423692/150‑1 (MQ=255)
                                 tCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGgcgc                                                                                                                      <  1:2455538/150‑1 (MQ=255)
                                   tACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCaa                                                                                                                    >  2:1569546/1‑150 (MQ=255)
                                   tACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCaa                                                                                                                    >  1:453191/1‑150 (MQ=255)
                                    aCCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAATCCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACCGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAAt                                                                                                                   >  2:685593/1‑150 (MQ=255)
                                     ccGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATg                                                                                                                  >  1:1444158/1‑150 (MQ=255)
                                     ccGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATg                                                                                                                  <  1:1286805/150‑1 (MQ=255)
                                       gCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCa                                                                                                                >  1:1078211/1‑150 (MQ=255)
                                         cTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAgg                                                                                                              >  1:25779/1‑150 (MQ=255)
                                            cTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCAt                                                                                                           >  1:299275/1‑150 (MQ=255)
                                              catcatGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTc                                                                                                         >  1:1740184/1‑150 (MQ=255)
                                              catcatGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTc                                                                                                         >  1:1748907/1‑150 (MQ=255)
                                               atcatGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTcc                                                                                                        <  1:572971/150‑1 (MQ=255)
                                                tcatGCGCCAGCCACTCGATATTAAAACCATCAACGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCg                                                                                                       <  1:1980086/150‑1 (MQ=255)
                                                 catGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCgg                                                                                                      >  1:713734/1‑150 (MQ=255)
                                                 catGTGCCAGCCATTCGATATTAAAACCATCAAGGGGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTAAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCgg                                                                                                      <  2:1189085/150‑1 (MQ=255)
                                                   tgtgCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGAt                                                                                                    <  1:1673695/150‑1 (MQ=255)
                                                   tgtgCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGAt                                                                                                    <  1:2449919/150‑1 (MQ=255)
                                                   tgtgCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTACTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGAt                                                                                                    >  1:1976414/1‑150 (MQ=255)
                                                     tgCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTc                                                                                                  >  1:1521314/1‑150 (MQ=255)
                                                     tgCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTc                                                                                                  >  1:702518/1‑150 (MQ=255)
                                                      gccagccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCa                                                                                                 >  2:425518/1‑150 (MQ=255)
                                                      gccagccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCa                                                                                                 >  1:63316/1‑150 (MQ=255)
                                                      gccagccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCa                                                                                                 >  2:2179077/1‑150 (MQ=255)
                                                        cagccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATc                                                                                               <  1:2270993/150‑1 (MQ=255)
                                                          gccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGc                                                                                             <  2:1927581/150‑1 (MQ=255)
                                                          gccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTAGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGc                                                                                             >  2:1057420/1‑150 (MQ=255)
                                                           ccaTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCa                                                                                            <  2:1051304/150‑1 (MQ=255)
                                                            caTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAg                                                                                           <  1:2367380/150‑1 (MQ=255)
                                                            caTTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAg                                                                                           <  1:2376139/150‑1 (MQ=255)
                                                              ttCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGAc                                                                                         <  1:909461/150‑1 (MQ=255)
                                                                cGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACAt                                                                                       >  2:260191/1‑150 (MQ=255)
                                                                cGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACAt                                                                                       >  2:2032111/1‑150 (MQ=255)
                                                                 gATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAAAGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATa                                                                                      <  2:662741/150‑1 (MQ=255)
                                                                   tatTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACa                                                                                    >  2:1955754/1‑150 (MQ=255)
                                                                    atTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAg                                                                                   <  2:1521314/150‑1 (MQ=255)
                                                                      tAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCt                                                                                 >  1:1586365/1‑150 (MQ=255)
                                                                       aaaaCCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTg                                                                                >  1:1322885/1‑150 (MQ=255)
                                                                           ccATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATAc                                                                            >  2:1652769/1‑150 (MQ=255)
                                                                           ccATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATAc                                                                            >  1:29601/1‑150 (MQ=255)
                                                                            cATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATAcg                                                                           >  2:1334095/1‑150 (MQ=255)
                                                                               cAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGt                                                                        <  1:1117356/150‑1 (MQ=255)
                                                                                aaGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTa                                                                       >  2:2226861/1‑150 (MQ=255)
                                                                                  ggTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTACCCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCCACGTTTCCAGGCGGCGGCGCAATGAAGGCATTCCGGATTCATCGCAGCCATACAGCTGATACGCGTAAt                                                                     >  2:553056/1‑150 (MQ=255)
                                                                                   gTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATc                                                                    <  1:636137/150‑1 (MQ=255)
                                                                                   gTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATc                                                                    <  2:299275/150‑1 (MQ=255)
                                                                                    tGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCt                                                                   <  1:766696/150‑1 (MQ=255)
                                                                                     gcgcGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCtt                                                                  >  1:2190772/1‑150 (MQ=255)
                                                                                      cgcgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGAATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTc                                                                 >  2:786456/1‑150 (MQ=255)
                                                                                       gcgAGATAATCCTGACGGGGGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTcc                                                                >  2:616749/1‑150 (MQ=255)
                                                                                          agaTAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGc                                                             <  1:2257796/150‑1 (MQ=255)
                                                                                          agaTAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTACTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGc                                                             <  2:1976414/150‑1 (MQ=255)
                                                                                             tAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGcacc                                                          <  1:156331/150‑1 (MQ=255)
                                                                                             tAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGcacc                                                          <  2:1908155/150‑1 (MQ=255)
                                                                                              aaTCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTCTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGcacca                                                         >  2:1484083/1‑150 (MQ=255)
                                                                                                tcatGACAGGCGTTATCACGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTATCCAGGCGGCGGCGCAATCAAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCcccacc                                                       >  2:1578188/4‑150 (MQ=255)
                                                                                                tCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCaccacc                                                       >  1:1628935/1‑150 (MQ=255)
                                                                                                 ccTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCAccacca                                                      >  2:706792/1‑150 (MQ=255)
                                                                                                  cTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAg                                                     <  2:1384821/150‑1 (MQ=255)
                                                                                                   tGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGc                                                    >  1:1651422/1‑150 (MQ=255)
                                                                                                        ggCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAAcc                                               >  1:444609/1‑150 (MQ=255)
                                                                                                        ggCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAAcc                                               >  1:1893248/1‑150 (MQ=255)
                                                                                                        ggCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCAGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAAcc                                               >  2:1469941/1‑150 (MQ=255)
                                                                                                           gTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACcgcg                                            >  2:1690591/1‑150 (MQ=255)
                                                                                                           gTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACcgcg                                            <  2:702518/150‑1 (MQ=255)
                                                                                                                   gCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTg                                    <  2:1314548/150‑1 (MQ=255)
                                                                                                                      cGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCa                                 >  2:1993978/1‑150 (MQ=255)
                                                                                                                       gCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACGTGCCAc                                <  2:1322885/150‑1 (MQ=255)
                                                                                                                        cTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCAcc                               >  1:581377/1‑150 (MQ=255)
                                                                                                                          aaCCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGt                             >  2:2263088/1‑150 (MQ=255)
                                                                                                                            ccAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTaa                           >  1:873111/1‑150 (MQ=255)
                                                                                                                               gcgcACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGtt                        <  2:651850/150‑1 (MQ=255)
                                                                                                                                 gcACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTa                      >  2:1381709/1‑150 (MQ=255)
                                                                                                                                 gcACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTa                      <  1:2093732/150‑1 (MQ=255)
                                                                                                                                 gcACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTa                      <  1:1681373/150‑1 (MQ=255)
                                                                                                                                   gcaaaCTTGCCGTTAAACCGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATc                    >  1:2481016/4‑150 (MQ=255)
                                                                                                                                    caaaCTTGCCGTTAAACCGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCg                   >  1:1593378/3‑150 (MQ=255)
                                                                                                                                    cTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCg                   >  1:560469/1‑150 (MQ=255)
                                                                                                                                     tgaCTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGc                  >  1:2359322/3‑150 (MQ=255)
                                                                                                                                       aCTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGccc                <  1:1166346/150‑1 (MQ=255)
                                                                                                                                        cTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCg               >  1:2287666/1‑150 (MQ=255)
                                                                                                                                           gCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGtt            >  1:406730/1‑150 (MQ=255)
                                                                                                                                              gTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTgcg         >  1:1331058/1‑150 (MQ=255)
                                                                                                                                              gTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTgcg         <  1:1559522/150‑1 (MQ=255)
                                                                                                                                                    cGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCa   >  1:496496/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCAT  >  NC_000913/3215848‑3216146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑