Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,336,002 C→G 5.8% A165P (GCA→CCA)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,0020CG 5.8% 402.2 / 11.8 120A165P (GCA→CCA) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (55/58);  new base G (5/2);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 124 total aligned reads displayed.

CGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTAC  >  NC_000913/3335855‑3336151
                                                                                                                                                   |                                                                                                                                                     
cgatacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTgcgc                                                                                                                                                     >  1:2258281/1‑150 (MQ=255)
 gatacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGcc                                                                                                                                                    <  2:2331827/150‑1 (MQ=255)
   tacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCaa                                                                                                                                                  >  2:1291362/1‑150 (MQ=255)
     cgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACg                                                                                                                                                >  1:711020/1‑150 (MQ=255)
       atCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCt                                                                                                                                              >  1:2095688/1‑150 (MQ=255)
        tCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTg                                                                                                                                             <  1:793730/150‑1 (MQ=255)
          ggTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGAc                                                                                                                                           <  2:1718346/150‑1 (MQ=255)
          ggTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAAAAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGAc                                                                                                                                           <  2:2288078/150‑1 (MQ=255)
             gCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACttt                                                                                                                                        >  2:1187784/1‑150 (MQ=255)
              cccTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGGTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTa                                                                                                                                       <  2:2141167/150‑1 (MQ=255)
               ccTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCAGCGTTGGCGCAACGGTGAccatca                                                                                                                                      <  1:56606/150‑6 (MQ=255)
               ccTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTAt                                                                                                                                      >  2:2231382/1‑150 (MQ=255)
                cTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATc                                                                                                                                     >  1:2133237/1‑150 (MQ=255)
                cTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATc                                                                                                                                     <  2:498977/150‑1 (MQ=255)
                    ccGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATc                                                                                                                                 >  1:1781045/1‑150 (MQ=255)
                      gCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCAc                                                                                                                               <  2:1641875/150‑1 (MQ=255)
                      gCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATAGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCAc                                                                                                                               >  2:710977/1‑150 (MQ=255)
                          aTTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGata                                                                                                                           <  2:845704/150‑1 (MQ=255)
                            tttCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATAtg                                                                                                                         <  1:666839/150‑1 (MQ=255)
                                 cACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCAc                                                                                                                    >  2:1338994/1‑150 (MQ=255)
                                 cACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCAc                                                                                                                    >  2:33918/1‑150 (MQ=255)
                                  aCCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCAcc                                                                                                                   <  2:1232050/150‑1 (MQ=255)
                                  aCCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCAcc                                                                                                                   <  2:2250332/150‑1 (MQ=255)
                                   cccAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCt                                                                                                                  >  1:1211276/1‑150 (MQ=255)
                                     cAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCtta                                                                                                                >  2:39948/1‑149 (MQ=255)
                                      aGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTc                                                                                                               <  2:660735/150‑1 (MQ=255)
                                          tAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAAc                                                                                                           <  1:649843/150‑1 (MQ=255)
                                            aTCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATATTCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGAAATGTGCACCTTTCAAACgc                                                                                                         >  1:2478868/1‑149 (MQ=255)
                                             tCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGAc                                                                                                        >  2:1464080/1‑150 (MQ=255)
                                              cAGGAAGTTCGCGGTATCGACGACTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTCCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGAcc                                                                                                       <  2:2222706/150‑1 (MQ=255)
                                               aGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCa                                                                                                      >  2:374902/1‑150 (MQ=255)
                                                ggAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCAt                                                                                                     >  1:579136/1‑150 (MQ=255)
                                                 gAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATc                                                                                                    <  1:2230515/150‑1 (MQ=255)
                                                     ttCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACg                                                                                                <  1:1792924/150‑1 (MQ=255)
                                                        gcgGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGaa                                                                                             >  1:229698/1‑150 (MQ=255)
                                                         cgGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAg                                                                                            <  2:2306915/150‑1 (MQ=255)
                                                          ggTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGc                                                                                           >  2:2466905/1‑150 (MQ=255)
                                                          ggTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGc                                                                                           <  1:1089836/150‑1 (MQ=255)
                                                             aTCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCttt                                                                                        >  2:2024232/1‑150 (MQ=255)
                                                                gacgaTTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAAc                                                                                     >  1:1109861/1‑150 (MQ=255)
                                                                gacgaTTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAAc                                                                                     <  2:729465/150‑1 (MQ=255)
                                                                gacgaTTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAAc                                                                                     >  1:1471139/1‑150 (MQ=255)
                                                                cacgaTTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTCCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAAc                                                                                     <  2:459847/149‑1 (MQ=255)
                                                                  cgaTTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGt                                                                                   >  2:1809222/1‑150 (MQ=255)
                                                                     tttCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAc                                                                                >  1:951015/1‑150 (MQ=255)
                                                                     tttCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAc                                                                                <  1:1203555/150‑1 (MQ=255)
                                                                      ttCCGGTTAACTCGCTGCGTTTTCAATAATCGTGTTTCCTTCAGCCAGGTTTGCATCACACATGATGTTAACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAcc                                                                               >  2:1672197/1‑150 (MQ=255)
                                                                       tCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACct                                                                              <  1:1187784/150‑1 (MQ=255)
                                                                          ggTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACcttct                                                                           >  1:1812923/1‑150 (MQ=255)
                                                                          ggTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACcttct                                                                           >  1:1561973/1‑150 (MQ=255)
                                                                          ggTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACcttct                                                                           >  2:2218189/1‑150 (MQ=255)
                                                                          ggTTCACGCGCGGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACTCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACcttct                                                                           <  2:2337306/150‑1 (MQ=255)
                                                                           gTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACcttctt                                                                          >  1:1067297/1‑150 (MQ=255)
                                                                           gTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACcttctt                                                                          <  1:1984839/150‑1 (MQ=255)
                                                                                cgcgCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGt                                                                     >  2:1998378/1‑150 (MQ=255)
                                                                                  cgcTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGttt                                                                   <  2:1812923/150‑1 (MQ=255)
                                                                                   gcTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTg                                                                  <  2:1425451/150‑1 (MQ=255)
                                                                                   gcTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTg                                                                  <  2:1434051/150‑1 (MQ=255)
                                                                                      gCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATg                                                               >  1:1393407/1‑150 (MQ=255)
                                                                                      gCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATg                                                               >  1:1563124/1‑150 (MQ=255)
                                                                                       cGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATgg                                                              <  2:934618/150‑1 (MQ=255)
                                                                                        gTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCTCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGt                                                             >  2:1595135/1‑150 (MQ=255)
                                                                                             cAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCCCCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCgcgc                                                        >  1:2353860/1‑150 (MQ=255)
                                                                                             cAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGGAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCgcgc                                                        <  2:1781045/150‑1 (MQ=255)
                                                                                             cAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCgcgc                                                        >  1:35694/1‑150 (MQ=255)
                                                                                               ataatCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCTACGGATGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCt                                                      <  2:1317765/150‑1 (MQ=255)
                                                                                                taatCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTa                                                     >  2:427324/1‑150 (MQ=255)
                                                                                                      tggtgCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGtt                                               >  1:108790/1‑150 (MQ=255)
                                                                                                      tggtgCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGtt                                               >  2:1825253/1‑150 (MQ=255)
                                                                                                       ggtgCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTc                                              <  2:606765/150‑1 (MQ=255)
                                                                                                         tgCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGa                                            >  2:956357/1‑150 (MQ=255)
                                                                                                          gCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAg                                           >  1:1747912/1‑150 (MQ=255)
                                                                                                          gCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAg                                           <  1:215238/150‑1 (MQ=255)
                                                                                                               ccGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAg                                      <  2:420170/150‑1 (MQ=255)
                                                                                                                  ccAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGaaa                                   <  1:721734/150‑1 (MQ=255)
                                                                                                                      ggTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtgtg                               <  2:1471139/150‑1 (MQ=255)
                                                                                                                      ggTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtgtg                               <  1:1526624/150‑1 (MQ=255)
                                                                                                                      ggTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtgtg                               <  1:442434/150‑1 (MQ=255)
                                                                                                                       gTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATgtgt                              <  1:299340/150‑1 (MQ=255)
                                                                                                                        ttGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTa                             <  1:430411/150‑1 (MQ=255)
                                                                                                                         tGCAGCACACATGATGGTCACCTTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAATCGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAg                            <  2:435489/150‑1 (MQ=255)
                                                                                                                         tGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAg                            <  1:2115418/150‑1 (MQ=255)
                                                                                                                          gcagcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGa                           <  1:2222821/150‑1 (MQ=255)
                                                                                                                            agcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCAAGGCCAGAAATGTGTAGATc                         <  2:2338903/150‑1 (MQ=255)
                                                                                                                                  caTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGCCCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCgg                   >  1:108454/1‑150 (MQ=255)
                                                                                                                                  caTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCgg                   >  1:1338742/1‑150 (MQ=255)
                                                                                                                                       tgGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGAcgcg              <  1:1338994/150‑1 (MQ=255)
                                                                                                                                       aagTCAGCGTTGGCGCAACGGTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTTACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGAcgcg              >  1:822002/3‑150 (MQ=255)
                                                                                                                                       aagTCAGCGTTGGCGCAACGGTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCATAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGaaccc              >  2:310102/3‑146 (MQ=255)
                                                                                                                                        ggTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACgcgc             >  2:564882/1‑150 (MQ=255)
                                                                                                                                         gTCAGCGTTGGCGCAACGGTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCa            >  2:2133800/1‑150 (MQ=255)
                                                                                                                                         gTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCa            <  1:1947152/150‑1 (MQ=255)
                                                                                                                                         gTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCa            <  2:579136/150‑1 (MQ=255)
                                                                                                                                          tCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCAc           >  1:1081134/1‑150 (MQ=255)
                                                                                                                                            aCCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCg         <  1:1595135/150‑1 (MQ=255)
                                                                                                                                             ccGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCCCCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGa        >  1:1719878/1‑150 (MQ=255)
                                                                                                                                              cGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGAt       <  2:951015/150‑1 (MQ=255)
                                                                                                                                                ttGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCg     <  1:1998378/150‑1 (MQ=255)
                                                                                                                                                  gcgcCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTa   <  1:2466905/150‑1 (MQ=255)
                                                                                                                                                   cgcCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTAc  >  2:1055022/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTAC  >  NC_000913/3335855‑3336151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑