BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,396,962	T→G	5.9%	R278R (CGA→CGC)	rng ←	RNase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,396,962	0	T	G	5.9%	558.1 / 22.3	187	R278R (CGA→CGC)	rng	RNase G
Reads supporting (aligned to +/- strand): ref base T (83/93); new base G (9/2); total (92/95)
Fisher's exact test for biased strand distribution p-value = 3.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 195 total aligned reads displayed.

TCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTC  >  NC_000913/3396814‑3397111

tCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGcgctc                                                                                                                                                      <  1:12527/150‑1 (MQ=255)

  gCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGcgctcgc                                                                                                                                                    <  1:1293914/150‑1 (MQ=255)

   cTTAAATATTGGTATTGAAAATGGTGTAGTCCAGATTGCGATGACCGACAAACGCTCAGCTATTGATGTCCACGGTGGTAATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGcgctcgct                                                                                                                                                   >  2:521835/1‑150 (MQ=255)

    ttCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTg                                                                                                                                                  >  2:940673/1‑150 (MQ=255)

     tCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                                                                                                                 >  1:1832625/1‑150 (MQ=255)

      cAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGa                                                                                                                                                >  1:1474652/1‑150 (MQ=255)

       aaTATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGAt                                                                                                                                               >  1:2211309/1‑150 (MQ=255)

        atatTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGAtt                                                                                                                                              >  2:37879/1‑150 (MQ=255)

         aatTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGAGCGCTGGaacg                                                                                                                                             <  1:1077402/149‑4 (MQ=255)

           ttGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATATCg                                                                                                                                           <  2:1470613/150‑1 (MQ=255)

             ggTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGtt                                                                                                                                         >  1:1170762/1‑150 (MQ=255)

              gTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGttt                                                                                                                                        >  1:25914/1‑150 (MQ=255)

                  tGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGa                                                                                                                                    <  2:2120818/150‑1 (MQ=255)

                  tGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGa                                                                                                                                    >  2:2175734/1‑150 (MQ=255)

                  tGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGa                                                                                                                                    >  1:1224709/1‑150 (MQ=255)

                  tGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGa                                                                                                                                    <  2:1529072/150‑1 (MQ=255)

                   gAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGAc                                                                                                                                   >  2:295020/1‑150 (MQ=255)

                    aaaaTGGTGTCGTCCAGATTGCGATGACCGACAAACTCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACa                                                                                                                                  <  2:753357/150‑1 (MQ=255)

                    aaaaTGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACa                                                                                                                                  <  2:1538780/150‑1 (MQ=255)

                          gtgtCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAag                                                                                                                            <  2:1394918/150‑1 (MQ=255)

                              cgtcCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGAt                                                                                                                        >  2:2330926/1‑150 (MQ=255)

                              cgtcCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTCCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGAt                                                                                                                        >  2:22346/1‑150 (MQ=255)

                              cgtcCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTACGGTCTGGTCGATAATGAGATAACCCCCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGAt                                                                                                                        >  2:2322525/1‑150 (MQ=255)

                               gtcCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATATCTACATTATTTGAGTTCTACTTTGCTTTCCAGCTCTCTCTGTATTTCTTTTTCTACATCAAAGAGTTc                                                                                                                       <  2:1839541/150‑1 (MQ=255)

                                tcCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCg                                                                                                                      <  1:1035816/150‑1 (MQ=255)

                                   aGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGaaa                                                                                                                   >  1:476016/1‑150 (MQ=255)

                                        gCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCgg                                                                                                              >  1:762533/1‑150 (MQ=255)

                                        gCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCgg                                                                                                              >  1:2113842/1‑150 (MQ=255)

                                          gATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCt                                                                                                            <  1:1238982/150‑1 (MQ=255)

                                           aTGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTg                                                                                                           <  2:1966596/150‑1 (MQ=255)

                                             gACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGc                                                                                                         >  2:848582/1‑150 (MQ=255)

                                               ccGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGt                                                                                                       >  2:2410675/1‑150 (MQ=255)

                                               ccGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGt                                                                                                       >  1:2447436/1‑150 (MQ=255)

                                                 gACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTcc                                                                                                     >  2:600141/1‑150 (MQ=255)

                                                   cAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCtg                                                                                                   <  1:37879/150‑1 (MQ=255)

                                                   cAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCtg                                                                                                   <  1:1463261/150‑1 (MQ=255)

                                                     aaCGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCtgtg                                                                                                 >  2:1445604/1‑150 (MQ=255)

                                                      aCGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTgtgt                                                                                                <  1:491748/150‑1 (MQ=255)

                                                             ggTATTGATGTCAACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTc                                                                                         >  2:157304/1‑150 (MQ=255)

                                                              gTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTcc                                                                                        <  2:1276400/150‑1 (MQ=255)

                                                                aTTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAg                                                                                      <  1:592/150‑1 (MQ=255)

                                                                  tGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTTGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAgct                                                                                    >  1:1343946/1‑150 (MQ=255)

                                                                  tGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAgct                                                                                    <  1:970135/150‑1 (MQ=255)

                                                                   gATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGTCATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAgctt                                                                                   >  2:248913/1‑150 (MQ=255)

                                                                     tGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCATTTCTACTTTGCTTTCCATCGCTCGCTTTATTTCGTTTTCGACTTCAAAGAGATCTAAAATCGTCTTGGGTCCTGTGTAATTTTCCAgattgc                                                                                 <  2:725913/150‑1 (MQ=255)

                                                                     tGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAgcttgc                                                                                 >  1:986319/1‑150 (MQ=255)

                                                                     tGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTCCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAgcttgc                                                                                 <  1:22346/150‑1 (MQ=255)

                                                                        ccACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGcttgcttg                                                                              <  1:1110053/150‑1 (MQ=255)

                                                                         cACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGt                                                                             >  1:120855/1‑150 (MQ=255)

                                                                                 tCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCgg                                                                     <  2:1170762/150‑1 (MQ=255)

                                                                                  cATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGGTCGCTGGATTTCGTTTTCGACATCATAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATGTCggg                                                                    <  1:1595048/150‑1 (MQ=255)

                                                                                  cATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCggg                                                                    >  2:399918/1‑150 (MQ=255)

                                                                                    tCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGaa                                                                  >  2:1927441/1‑150 (MQ=255)

                                                                                    tCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGaa                                                                  >  1:2045964/1‑150 (MQ=255)

                                                                                       cTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGt                                                               <  2:985933/150‑1 (MQ=255)

                                                                                       cTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGt                                                               <  2:1034995/150‑1 (MQ=255)

                                                                                           ggTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCAAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTc                                                           <  1:842696/150‑1 (MQ=255)

                                                                                              cTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGa                                                        >  2:620782/1‑150 (MQ=255)

                                                                                              cTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGa                                                        >  1:320807/1‑150 (MQ=255)

                                                                                                ggTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAgg                                                      <  1:521835/150‑1 (MQ=255)

                                                                                                ggTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAgg                                                      >  2:1781677/1‑150 (MQ=255)

                                                                                                 gTCGATAATCATATAACCAAAGGATTTAAGATCTACTTTAACTTCCAGCTCTCCCTAGATTTAATATTAAACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGt                                                     >  2:2484062/1‑150 (MQ=255)

                                                                                                  tCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTg                                                    >  1:2127460/1‑150 (MQ=255)

                                                                                                  tCGATAATGAGATAACCACCGGATTTCAGGTCTACTTTGCTTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGATATCTAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGTGAATGTACTCAGAGGTg                                                    <  2:1898186/150‑1 (MQ=255)

                                                                                                   cGATAATGAGATAACCACTGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGa                                                   >  1:154393/1‑150 (MQ=255)

                                                                                                          tagaTAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAg                                            <  1:1041379/149‑1 (MQ=255)

                                                                                                          tagaTAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAg                                            <  2:1046662/149‑1 (MQ=255)

                                                                                                          gagaTAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAg                                            <  1:399918/150‑1 (MQ=255)

                                                                                                              tAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAAc                                        >  2:582471/1‑150 (MQ=255)

                                                                                                              tAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAAc                                        <  2:2211309/150‑1 (MQ=255)

                                                                                                               aaCCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACg                                       >  1:888736/1‑150 (MQ=255)

                                                                                                                    ccGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCg                                  <  2:338744/150‑1 (MQ=255)

                                                                                                                    ccGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCg                                  <  1:1194739/150‑1 (MQ=255)

                                                                                                                        aTTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAg                              <  2:1765524/150‑1 (MQ=255)

                                                                                                                        aTTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAg                              <  2:2071005/150‑1 (MQ=255)

                                                                                                                         tttCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGt                             >  1:2187775/1‑150 (MQ=255)

                                                                                                                            cAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAg                          <  2:476016/150‑1 (MQ=255)

                                                                                                                               ttCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCg                       <  2:388864/150‑1 (MQ=255)

                                                                                                                                 cTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAATGCTTCGTAAGTCAGGCGTg                     <  2:124144/150‑1 (MQ=255)

                                                                                                                                 cTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTg                     <  1:2175734/150‑1 (MQ=255)

                                                                                                                                   aCTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCTTGAATGTACTCCGAGGTGAACTCTAGTAACTTTTCGTAAGTCATGCTTGAg                   <  2:1224709/150‑1 (MQ=255)

                                                                                                                                   aCTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAg                   >  2:1818336/1‑150 (MQ=255)

                                                                                                                                    cTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGt                  >  2:1420942/1‑150 (MQ=255)

                                                                                                                                     tttGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTc                 <  1:1927441/150‑1 (MQ=255)

                                                                                                                                      acgaaatCCAGCGAGCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCa                >  1:1065333/7‑150 (MQ=255)

                                                                                                                                       cgaaatCCAGCGAGCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCaa               >  1:1739322/6‑150 (MQ=255)

                                                                                                                                         cGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAAcg             >  2:2430042/1‑150 (MQ=255)

                                                                                                                                         cGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAAcg             <  1:1856353/150‑1 (MQ=255)

                                                                                                                                         cGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAAcg             >  1:784018/1‑150 (MQ=255)

                                                                                                                                          gTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAAcgc            >  2:11887/1‑150 (MQ=255)

                                                                                                                                          gTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAAcgc            >  1:664584/1‑150 (MQ=255)

                                                                                                                                            tCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGa          >  2:1626607/1‑150 (MQ=255)

                                                                                                                                               aGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATg       <  2:498397/150‑1 (MQ=255)

                                                                                                                                               aGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATg       >  2:677277/1‑150 (MQ=255)

                                                                                                                                               aGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATg       >  1:136994/1‑150 (MQ=255)

                                                                                                                                               aGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATg       <  2:154393/150‑1 (MQ=255)

                                                                                                                                                gcgcTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTATTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGc      <  2:873229/150‑1 (MQ=255)

                                                                                                                                                  gctcgctGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCgg    >  2:1125848/1‑150 (MQ=255)

                                                                                                                                                  gctcgctGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCgg    <  1:2368590/150‑1 (MQ=255)

                                                                                                                                                    tcgctGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTc  >  1:740366/1‑150 (MQ=255)

TCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTCATCTCGGGAATGTACTCCGAGGTGAACTCAAGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTC  >  NC_000913/3396814‑3397111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`