BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,648,130	A→T	9.5%	noncoding (68/84 nt)	agrA →	small RNA AgrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,130	0	A	T	9.5%	510.8 / 29.5	176	noncoding (68/84 nt)	agrA	small RNA AgrA
Reads supporting (aligned to +/- strand): ref base A (66/93); new base T (6/11); total (72/104)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

ACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647981‑3648278

aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCa                                                                                                                                                      <  2:1095532/150‑1 (MQ=255)

  tGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCaaa                                                                                                                                                    >  2:202807/1‑150 (MQ=255)

   ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAg                                                                                                                                                   >  1:493129/1‑150 (MQ=255)

       aaTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCgg                                                                                                                                               <  2:1758132/150‑1 (MQ=255)

        aTCATACAGAACATGCACGTTATAAAACCAGCAATTCCTTGCAATTTTTTCAGTTTGGTATTAGTATTGATGTGTTAGATTATTTCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                              <  2:193920/150‑1 (MQ=21)

          cATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttt                                                                                                                                            <  2:2021173/150‑1 (MQ=255)

            tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttt                                                                                                                                          >  2:2111923/1‑150 (MQ=255)

             aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                         <  2:2393302/150‑1 (MQ=38)

             aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                         <  2:1966728/150‑1 (MQ=38)

             aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  1:2126315/150‑1 (MQ=255)

             aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                         <  1:2375445/150‑1 (MQ=38)

              cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  1:316105/150‑1 (MQ=255)

              cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        >  2:673162/1‑150 (MQ=255)

              cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  2:855826/150‑1 (MQ=255)

              cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         >  1:773938/1‑149 (MQ=255)

               aGTACATGCCCGTTATAAAACCAGCTCTTCCTTGCAATATTTTCATCATGGTATTAGCATTGATGCGTTAGATTATCCCTATCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                       <  1:81887/150‑3 (MQ=21)

                gatcttGCATGTTATAAAACCAGGACGTCCTTTCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                      <  2:791760/145‑1 (MQ=255)

                gTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                       <  2:277775/150‑3 (MQ=38)

                gTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                      <  2:1332479/150‑1 (MQ=255)

                   cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGttttttactttc                                                                                                                                   <  1:1075458/150‑7 (MQ=38)

                   cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATc                                                                                                                                   >  2:421847/1‑150 (MQ=255)

                    gtGCTGGTTTTATAACCTGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATcc                                                                                                                                  >  2:1291244/2‑150 (MQ=255)

                            tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCgg                                                                                                                          <  1:159095/150‑1 (MQ=255)

                              aaaaaCCAGCAATTTCTTGCAATTTTTTCCCTTTGGTATTAGTATTGATGTGTTAGATTAGTCCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAc                                                                                                                        <  2:51354/149‑1 (MQ=11)

                                aaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACaa                                                                                                                      >  2:2220655/1‑150 (MQ=255)

                                 aaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAg                                                                                                                     <  1:1745940/150‑1 (MQ=255)

                                   ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGc                                                                                                                   <  2:1576336/150‑1 (MQ=255)

                                   acAGCACCTCCTTGCAATTTTTTCCCTTTGGTATTAGCATTGATGCGTTAGATGATTGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcctcct                                                                                                                   <  2:1593590/149‑6 (MQ=17)

                                    cAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCt                                                                                                                  <  1:294363/150‑1 (MQ=255)

                                     aGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCTCGGAAAACGCGGGTGATTTAAATCGGGATTCGGACAAGGCtt                                                                                                                 >  1:314462/1‑150 (MQ=37)

                                     aGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCtt                                                                                                                 <  1:2393624/150‑1 (MQ=255)

                                       cACGTCCTTTCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGTCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGTAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTaa                                                                                                               <  2:748242/150‑1 (MQ=255)

                                       cACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTaa                                                                                                               <  2:1460693/150‑1 (MQ=255)

                                       cACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTaa                                                                                                               >  1:692975/1‑150 (MQ=255)

                                       cACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGATTaa                                                                                                               >  1:582169/1‑150 (MQ=255)

                                            ccTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGa                                                                                                          >  1:1959673/1‑150 (MQ=255)

                                             cTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAc                                                                                                         >  2:1797153/1‑150 (MQ=255)

                                             cTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAc                                                                                                         >  1:2158299/1‑150 (MQ=255)

                                                 cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                     >  2:1228814/1‑150 (MQ=255)

                                                   aTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAc                                                                                                   <  2:2429618/150‑1 (MQ=255)

                                                   aTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAc                                                                                                   >  2:2474497/1‑150 (MQ=255)

                                                   aTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAc                                                                                                   >  1:1205472/1‑150 (MQ=255)

                                                     aGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAccc                                                                                                 <  1:1489226/150‑1 (MQ=255)

                                                     aGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAccc                                                                                                 <  1:450653/150‑1 (MQ=255)

                                                       tttCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAg                                                                                               <  1:1291244/150‑1 (MQ=255)

                                                        ttCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGt                                                                                              <  1:482217/150‑1 (MQ=255)

                                                        ttCAGTATGGTATAAGCATTGATGCGTTTGATGAGGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGt                                                                                              <  2:1440388/150‑1 (MQ=255)

                                                          cAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGa                                                                                            >  1:1166229/1‑150 (MQ=255)

                                                          cAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGa                                                                                            >  2:2309317/1‑150 (MQ=255)

                                                            gTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                          >  2:1270463/1‑150 (MQ=255)

                                                            gTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                          >  1:1743140/1‑150 (MQ=255)

                                                             tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCgg                                                                                                                                               >  2:943598/1‑96 (MQ=255)

                                                             tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCgg                                                                                                                                               <  1:943598/96‑1 (MQ=255)

                                                              aTGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGt                                                                                        <  1:661648/150‑1 (MQ=255)

                                                               tGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGta                                                                                       <  2:1829042/150‑1 (MQ=255)

                                                               tGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGta                                                                                       <  2:1342346/150‑1 (MQ=255)

                                                               tGGTATTAGCATTGATGCGTTAGATGATGGATATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGta                                                                                       <  2:225079/150‑1 (MQ=255)

                                                                 gTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtata                                                                                     <  2:2299403/150‑1 (MQ=255)

                                                                  tATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatat                                                                                    >  1:172476/1‑150 (MQ=255)

                                                                   aTTAGCATTGATGCGTTAGATGATGACTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                   >  2:363579/1‑150 (MQ=255)

                                                                       gCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATc                                                                               <  1:1765490/150‑1 (MQ=255)

                                                                       gCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATc                                                                               <  1:365807/150‑1 (MQ=255)

                                                                        cATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                              <  2:493129/150‑1 (MQ=255)

                                                                              tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                        <  2:841045/150‑1 (MQ=255)

                                                                               gCGTTAGATGATGGCCATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGc                                                                       <  1:997979/150‑1 (MQ=255)

                                                                                     gatgatGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGatata                                                                 >  1:2446898/1‑150 (MQ=255)

                                                                                             cTATCTAACTCCAGTCAGAGCCACCATCTCATGGATTTAAAGT‑AAAAATCCGACGCAAAGTAGGTTTTTTTACATGCGGAGTCGTACATGGCTTAATATGACGATGACCGTGTGTAAGTATATAAATCGTCACTGGGATTTATTCCGAAg                                                         <  2:2088657/150‑1 (MQ=255)

                                                                                                tctcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGc                                                      >  1:2000676/1‑150 (MQ=255)

                                                                                                   cACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctcc                                                   <  2:24410/150‑1 (MQ=255)

                                                                                                   cACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGAGATATATACCGAAGTGctcc                                                   >  2:785056/1‑150 (MQ=255)

                                                                                                    aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccc                                                  <  2:1743140/150‑1 (MQ=255)

                                                                                                      tCCAGTCAGAGCCACCAACTCAGAACTGGAAAGT‑AAAAAAACGACGAAAATTCGGTTTTTTTACATCCGGATTCGGAGAAGGATTAATATAACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccctc                                                >  2:1428287/1‑150 (MQ=255)

                                                                                                       ccAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATACACCGAAGTGctccctcc                                               >  1:168565/1‑150 (MQ=255)

                                                                                                        cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCTAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                         >  2:1412270/1‑139 (MQ=255)

                                                                                                        cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCTAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                         <  1:1412270/139‑1 (MQ=255)

                                                                                                        cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                              <  1:2220655/150‑1 (MQ=255)

                                                                                                          gTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                            <  1:232863/150‑1 (MQ=255)

                                                                                                              gagCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCt                                        <  1:1012626/150‑1 (MQ=255)

                                                                                                               agCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTg                                       <  2:16390/150‑1 (MQ=255)

                                                                                                                  caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                    <  1:2474497/150‑1 (MQ=255)

                                                                                                                  caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATCAAGATGACCCAGTCAATGAATATAAATCGTCACTGAGATATATACAGAAGTGCTCCCTCCCCAAGCTgaat                                    >  1:871690/1‑149 (MQ=25)

                                                                                                                   accaACTATTGGCTGGAAATT‑AAAAAAGCGACGCAATGTCGGTTTTTTTACATCCTGATTAGGATAAGGCTTAATATGACGATGACGAAGTTAAAGTATATAAATCGTCACTTCGATATATACGGAATTGCTCCCTCAGCCAGCTgaagt                                   <  2:2040899/150‑2 (MQ=255)

                                                                                                                         tCAGGGCTGGAAAGT‑AAAAAACCGACTCAAAGTCTGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                             <  1:1270463/150‑1 (MQ=255)

                                                                                                                           aGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTa                           <  1:673162/150‑1 (MQ=255)

                                                                                                                            gggCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTaa                          >  1:1223771/1‑150 (MQ=255)

                                                                                                                            gggCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCAGACAACGATTAAAATGACGATCAACCAGAAAAAGTATAGAAATAAACAATGCGATATATAACCAAGTGGTACCTCAGCAAGCTGAAGAAATCGATaa                          >  2:1250391/1‑150 (MQ=16)

                                                                                                                                 ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                     >  1:208398/1‑150 (MQ=255)

                                                                                                                                   gatgtaaaaaaaCCGACTTTGGGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                    >  1:2415806/7‑150 (MQ=255)

                                                                                                                                   aaaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                   <  1:2022465/150‑1 (MQ=255)

                                                                                                                                     aGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACGCAGTGAAAGTATATAAATCGTCACTGCGATATATAGCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCaa                 <  1:457102/150‑1 (MQ=255)

                                                                                                                                      gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                >  1:193249/1‑150 (MQ=255)

                                                                                                                                       taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                >  1:671741/3‑150 (MQ=255)

                                                                                                                                        aaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATg               >  1:112624/2‑150 (MQ=255)

                                                                                                                                         aaaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTCAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGt              >  2:1879445/1‑150 (MQ=255)

                                                                                                                                          aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt             <  2:692975/150‑1 (MQ=255)

                                                                                                                                          aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt             >  1:85378/1‑150 (MQ=255)

                                                                                                                                             aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGGAATCGCTAATTCTTGCAATGTTAGc          <  2:1205472/150‑1 (MQ=255)

                                                                                                                                               ccGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCa        >  1:209954/1‑150 (MQ=255)

                                                                                                                                                    gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGc   <  2:324161/150‑1 (MQ=255)

                                                                                                                                                     cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt  <  1:1042670/150‑1 (MQ=255)

ACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647981‑3648278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`