BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,677,835	C→T	5.9%	R172R (CGG→CGA)	yhjG ←	AsmA family protein YhjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,677,835	0	C	T	5.9%	487.3 / 12.2	153	R172R (CGG→CGA)	yhjG	AsmA family protein YhjG
Reads supporting (aligned to +/- strand): ref base C (72/71); new base T (6/3); total (79/74)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 154 total aligned reads displayed.

CGCCTTCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGCTTTTCGAG  >  NC_000913/3677688‑3677984

 gcCTTCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGc                                                                                                                                                    <  2:670511/150‑1 (MQ=255)

     tCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCtt                                                                                                                                                <  2:471644/150‑1 (MQ=255)

     tCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCtt                                                                                                                                                <  1:1539529/150‑1 (MQ=255)

        ggCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGCTCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGAt                                                                                                                                             >  1:2001856/1‑150 (MQ=255)

        ggCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGAt                                                                                                                                             >  2:116360/1‑150 (MQ=255)

        ggCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAAGGGCGGATCGCCATTGAt                                                                                                                                             <  1:1161031/150‑1 (MQ=255)

         gCCTAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACGTTTCGATCCAGTAACTTCGGTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATc                                                                                                                                            <  1:1695344/150‑1 (MQ=255)

         gCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATc                                                                                                                                            <  1:2403/150‑1 (MQ=255)

         gCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAAGGGCGGATCGCCATTGAtg                                                                                                                                            <  2:2104648/150‑2 (MQ=255)

          ccAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCg                                                                                                                                           <  2:1026846/150‑1 (MQ=255)

          ccAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCg                                                                                                                                           >  1:167558/1‑150 (MQ=255)

          ccAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCg                                                                                                                                           >  1:1120677/1‑150 (MQ=255)

           cAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGa                                                                                                                                          >  1:183850/1‑150 (MQ=255)

           cAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAAGGGCGGATCGCCATTGAtgac                                                                                                                                          <  2:2423426/150‑4 (MQ=255)

            aaaaaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaa                                                                                                                                         >  1:1723889/1‑150 (MQ=255)

             aaaaCGTAATCGCCCACCTTTTCTTTCTCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaaa                                                                                                                                        <  2:1602875/150‑1 (MQ=255)

             aaaaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCACTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaaa                                                                                                                                        <  2:1295749/150‑1 (MQ=255)

              aaaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaaaa                                                                                                                                       <  1:1067216/150‑1 (MQ=255)

              aaaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaaaa                                                                                                                                       >  1:1752721/1‑150 (MQ=255)

              aaaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaaaa                                                                                                                                       <  2:388376/150‑1 (MQ=255)

              aaaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGaaaa                                                                                                                                       <  2:345304/150‑1 (MQ=255)

               aaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAg                                                                                                                                      <  1:223110/150‑1 (MQ=255)

               aaCGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGCCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAg                                                                                                                                      >  2:1994692/1‑150 (MQ=255)

                  ttAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGTACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAat                                                                                                                                   <  2:1285990/149‑1 (MQ=255)

                   tAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAata                                                                                                                                  <  2:134892/150‑1 (MQ=255)

                    aaTCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAatat                                                                                                                                 >  1:1915190/1‑150 (MQ=255)

                     aTCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAAtatt                                                                                                                                <  1:296233/150‑1 (MQ=255)

                      tCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAAtatta                                                                                                                               <  2:2226181/150‑1 (MQ=255)

                           cACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAg                                                                                                                          >  2:306420/1‑150 (MQ=255)

                              cTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCg                                                                                                                       >  1:759119/1‑150 (MQ=255)

                              cTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCg                                                                                                                       >  1:756940/1‑150 (MQ=255)

                                tttCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGaa                                                                                                                     <  2:853072/150‑1 (MQ=255)

                                  tCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAAc                                                                                                                   >  2:1142980/1‑150 (MQ=255)

                                  tCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAAc                                                                                                                   <  1:34035/150‑1 (MQ=255)

                                   cTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACg                                                                                                                  <  1:2360006/150‑1 (MQ=255)

                                    tttATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGa                                                                                                                 >  2:2311273/1‑150 (MQ=255)

                                      tATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGAcc                                                                                                               <  1:2101214/150‑1 (MQ=255)

                                       aTCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAACTCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCa                                                                                                              <  2:1561678/150‑1 (MQ=255)

                                       aTCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCa                                                                                                              >  1:924361/1‑150 (MQ=255)

                                          cGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGc                                                                                                           >  2:159025/1‑150 (MQ=255)

                                           gCTTTACCTTTCGCTCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCTAAAAGAATATTATCCAGCCGAAACGACCATGcc                                                                                                          <  2:682868/150‑1 (MQ=255)

                                              ttACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGAc                                                                                                       >  2:2270272/1‑150 (MQ=255)

                                                   tttCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCtt                                                                                                  <  2:923501/150‑1 (MQ=255)

                                                   tttCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCtt                                                                                                  >  1:369930/1‑150 (MQ=255)

                                                      cGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGc                                                                                               <  2:266688/150‑1 (MQ=255)

                                                      cGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGc                                                                                               >  2:2457595/1‑150 (MQ=255)

                                                      cGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGc                                                                                               <  1:2403478/150‑1 (MQ=255)

                                                       gATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCGTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGct                                                                                              >  2:471097/1‑149 (MQ=255)

                                                           cAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTcg                                                                                          <  1:1681534/150‑1 (MQ=255)

                                                           cAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTcg                                                                                          <  1:116360/150‑1 (MQ=255)

                                                             gTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTcgcg                                                                                        >  1:1827763/1‑150 (MQ=255)

                                                             gTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTcgcg                                                                                        <  1:1647743/150‑1 (MQ=255)

                                                                   tCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTg                                                                                  >  2:1629446/1‑150 (MQ=255)

                                                                   tCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTg                                                                                  <  2:1367790/150‑1 (MQ=255)

                                                                    cGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGt                                                                                 <  2:1752721/150‑1 (MQ=255)

                                                                       tGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTat                                                                              >  1:1191194/1‑150 (MQ=255)

                                                                          aCGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTatcat                                                                           >  1:484541/1‑150 (MQ=255)

                                                                             gCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGt                                                                        >  2:2005173/1‑150 (MQ=255)

                                                                               aGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTg                                                                      <  1:2270272/150‑1 (MQ=255)

                                                                                    cTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAg                                                                 <  2:753818/150‑1 (MQ=255)

                                                                                       gCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGAtt                                                              >  1:813661/1‑150 (MQ=255)

                                                                                       gCCTAAGGGAACAACAAAAATCTCCAGATCCGCATTGCTTACATTGTCATCAATGGCGATCCGCCCATGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTCTTAACATCGATGCCAAGAtt                                                              >  1:1280525/1‑150 (MQ=255)

                                                                                           aaGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAAc                                                          <  1:1994692/150‑1 (MQ=255)

                                                                                             cggATCAACAAAAATCTCAAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATACGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGt                                                        >  2:2119406/2‑150 (MQ=255)

                                                                                                aTCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCa                                                     <  1:1201927/150‑1 (MQ=255)

                                                                                                 tCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAg                                                    <  2:645510/150‑1 (MQ=255)

                                                                                                    acaaAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTa                                                 >  1:2057089/1‑150 (MQ=255)

                                                                                                       aaaaTCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTg                                              <  2:369930/150‑1 (MQ=255)

                                                                                                        aaaTCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGt                                             >  2:2411525/1‑150 (MQ=255)

                                                                                                            ctcCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCtt                                         >  2:1975204/1‑150 (MQ=255)

                                                                                                               cAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTc                                      <  2:1546113/150‑1 (MQ=255)

                                                                                                               cAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTc                                      >  2:2080630/1‑150 (MQ=255)

                                                                                                               cAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTc                                      <  1:673263/150‑1 (MQ=255)

                                                                                                                aGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCa                                     <  2:1191194/150‑1 (MQ=255)

                                                                                                                             ttACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCa                        <  2:1864788/150‑1 (MQ=255)

                                                                                                                              tACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAg                       >  2:327802/1‑150 (MQ=255)

                                                                                                                               aCTTTCTCATCAATGGCGATCCGCCCTTGATCGAAAAGAAAAATATCCACCCGAAAAGAACATACAGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAgg                      >  2:1652624/1‑150 (MQ=255)

                                                                                                                                cttttcgatcaAGGGCGGATCGCCATTGATCGAAAAGAATATTATCCAGCCGAACCGACCATGCCGCCGGCTTTGGATTAGCGTCTTTGTTATCAGCGTGGGCATGCTTAAACGGCCAGTTATTGTTCTTTTCGGCGAGGCGAATCAGgc                     >  2:849176/8‑150 (MQ=255)

                                                                                                                                 tttGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGgcg                    <  1:2199028/150‑1 (MQ=255)

                                                                                                                                    tcgatcaAGGGCGGATCGCCATTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGgcgcgc                 >  2:1910587/4‑150 (MQ=255)

                                                                                                                                    tcgatcaAGGGCGGATCGCCATTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGgcgcgc                 >  1:308547/4‑150 (MQ=255)

                                                                                                                                     tcatcaATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGcccgcg                <  1:552732/150‑1 (MQ=255)

                                                                                                                                      gatcaAGGGCGGATCGCCATTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGt               >  1:189612/2‑150 (MQ=255)

                                                                                                                                      catcaATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGt               >  1:632929/1‑150 (MQ=255)

                                                                                                                                      catcaATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGt               >  1:1323456/1‑150 (MQ=255)

                                                                                                                                      catcaATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGt               >  1:2339728/1‑150 (MQ=255)

                                                                                                                                       atcaATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTc              >  2:1359998/1‑150 (MQ=255)

                                                                                                                                       atcaAGGGCGGATCGCCATTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTc              >  2:1812659/1‑150 (MQ=255)

                                                                                                                                         caATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCgg            <  1:1507847/150‑1 (MQ=255)

                                                                                                                                         caATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCgg            >  1:1998539/1‑150 (MQ=255)

                                                                                                                                         caATGGCGATCCGCCCTTGATCG‑AAAGAATATTATCCAGCCGAAACGACCATGCCGCCGGCTTTGCATTAGCGTCGTTGTTTTCATCCTTGGCAAGATTAAACGTCCAATTTTTGTTCTTTTCAGAGAGGCCAATCAGGCGCGCGTCggg           >  2:2190798/1‑150 (MQ=255)

                                                                                                                                          aaTGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTc                                      >  1:1049137/1‑123 (MQ=255)

                                                                                                                                          aaTGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTc                                      <  2:1049137/123‑1 (MQ=255)

                                                                                                                                          aaTGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCggg           <  2:1324070/150‑1 (MQ=255)

                                                                                                                                          aaTGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCggg           <  2:183850/150‑1 (MQ=255)

                                                                                                                                           aTGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGc          <  2:1823757/150‑1 (MQ=255)

                                                                                                                                            tGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGGCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGCt         <  1:2033535/150‑1 (MQ=255)

                                                                                                                                              gCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGCttt       >  1:277689/1‑150 (MQ=255)

                                                                                                                                              gCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGCttt       >  1:1793798/1‑150 (MQ=255)

                                                                                                                                                gATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGCTTTTc     >  1:1111007/1‑150 (MQ=255)

CGCCTTCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATCAATGGCGATCCGCCCTTGATCGAAAAGAATATTATCCAGCCGAAACGACCATGCCGACGGCTTTGCATTCGCGTCTTTGTTATCATCGTTGGCAAGATTAAACGTCCAGTTATTGTTCTTTTCAGAGAGGCGAATCAGGCGCGCGTCGGGCTTTTCGAG  >  NC_000913/3677688‑3677984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`