BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,718,280	A→C	6.1%	D324A (GAC→GCC) ‡	ghrB →	glyoxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,718,280	0	A	C	6.1%	495.9 / 19.8	163	D324A (GAC→GCC) ‡	ghrB	glyoxylate reductase
Reads supporting (aligned to +/- strand): ref base A (78/75); new base C (7/3); total (85/78)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 165 total aligned reads displayed.

GCTCTCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTG  >  NC_000913/3718131‑3718429

 ctctCAATGGCCAACGTCGTCGCAGTACCGCATTTTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGAc                                                                                                                                                      <  2:610199/150‑1 (MQ=255)

 ctctCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGAc                                                                                                                                                      <  2:1672594/150‑1 (MQ=255)

 ctctCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGAc                                                                                                                                                      >  2:711026/1‑150 (MQ=255)

 ctctCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCACACGTCGCGGAc                                                                                                                                                      <  2:1582855/150‑1 (MQ=255)

    tcAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTaa                                                                                                                                                   >  2:1383189/1‑150 (MQ=255)

     cAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAg                                                                                                                                                  >  1:653124/1‑150 (MQ=255)

      aaTGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGc                                                                                                                                                 <  1:491251/150‑1 (MQ=255)

      aaTGGCCAACGTCGTCGCAGTACCACATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGc                                                                                                                                                 >  2:352482/1‑150 (MQ=255)

         ggCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCcgc                                                                                                                                              >  2:575788/1‑150 (MQ=255)

           ccAACGGCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACCCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGTAAAGGTTGAGAAGATCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGa                                                                                                                                            <  2:1265993/150‑1 (MQ=255)

            cAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGAc                                                                                                                                           <  2:2097892/150‑1 (MQ=255)

                 tcgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGt                                                                                                                                      <  2:1756838/150‑1 (MQ=255)

                 tcgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGt                                                                                                                                      <  1:208617/150‑1 (MQ=255)

                 tcgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGt                                                                                                                                      >  1:1930518/1‑150 (MQ=255)

                  cgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTa                                                                                                                                     >  2:724225/1‑150 (MQ=255)

                  cgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTa                                                                                                                                     <  2:1901402/150‑1 (MQ=255)

                   gtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTag                                                                                                                                    >  2:866042/1‑150 (MQ=255)

                   gtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTag                                                                                                                                    <  1:2155864/150‑1 (MQ=255)

                           aCCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGc                                                                                                                            >  2:1332568/1‑150 (MQ=255)

                              gCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCg                                                                                                                         <  2:1053258/150‑1 (MQ=255)

                                   ttGGATCTGCCACCCATGAGACGCGTTATGGCATGGTCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAAt                                                                                                                    >  1:397796/1‑150 (MQ=255)

                                   ttGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAAt                                                                                                                    >  1:1515294/1‑150 (MQ=255)

                                     ggATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCg                                                                                                                  <  2:2351482/150‑1 (MQ=255)

                                      gATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGAt                                                                                                                       >  2:514484/1‑144 (MQ=255)

                                      gATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGc                                                                                                                 >  1:1115118/1‑150 (MQ=255)

                                         cTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCg                                                                                                              <  1:1963615/150‑1 (MQ=255)

                                          tGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCgg                                                                                                             >  1:318419/1‑150 (MQ=255)

                                          tGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCgg                                                                                                             >  2:2382609/1‑150 (MQ=255)

                                                ccATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCtttt                                                                                                       <  1:1342842/150‑1 (MQ=255)

                                                 cATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTa                                                                                                      >  1:926283/1‑150 (MQ=255)

                                                  aTGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTAc                                                                                                     <  1:1371268/150‑1 (MQ=255)

                                                    gagaCGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTActc                                                                                                   >  1:2450091/1‑150 (MQ=255)

                                                       aCGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCttt                                                                                                <  2:1515294/150‑1 (MQ=255)

                                                        cgcgTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTa                                                                                               >  1:1304475/1‑150 (MQ=255)

                                                         gcgTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTAt                                                                                              >  2:1899520/1‑150 (MQ=255)

                                                         gcgTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTAt                                                                                              <  1:875812/150‑1 (MQ=255)

                                                           gTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTg                                                                                            >  1:221828/1‑150 (MQ=255)

                                                            ttATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTgg                                                                                           <  2:320260/150‑1 (MQ=255)

                                                            ttATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTgg                                                                                           <  2:318419/150‑1 (MQ=255)

                                                              atggcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGt                                                                                         <  2:1822163/150‑1 (MQ=255)

                                                                ggcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTg                                                                                       >  1:660450/1‑150 (MQ=255)

                                                                 gcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGc                                                                                      <  1:2210987/150‑1 (MQ=255)

                                                                 gcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTGGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGc                                                                                      <  1:1971958/150‑1 (MQ=255)

                                                                  catggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCa                                                                                     >  2:1486393/1‑150 (MQ=255)

                                                                   atggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAg                                                                                    <  2:372759/150‑1 (MQ=255)

                                                                   atggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAg                                                                                    <  2:269645/150‑1 (MQ=255)

                                                                    tggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGt                                                                                   <  1:352482/150‑1 (MQ=255)

                                                                      gccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTaa                                                                                 >  2:981919/1‑150 (MQ=255)

                                                                       ccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAc                                                                                <  2:802641/150‑1 (MQ=255)

                                                                       ccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAc                                                                                <  1:782507/150‑1 (MQ=255)

                                                                       ccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAc                                                                                <  1:1394076/150‑1 (MQ=255)

                                                                        cgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAct                                                                               >  1:1055959/1‑150 (MQ=255)

                                                                        cgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAct                                                                               <  2:922886/150‑1 (MQ=255)

                                                                          ccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCAGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAActgc                                                                             >  1:1114708/1‑150 (MQ=255)

                                                                           cTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAActgct                                                                            <  1:2049562/150‑1 (MQ=255)

                                                                           cTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTC‑TTATTGGGTTGCAGTAActgctg                                                                           <  2:759883/150‑1 (MQ=255)

                                                                             gtgCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGt                                                                          <  2:188369/150‑1 (MQ=255)

                                                                               gCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAg                                                                        >  1:1508919/1‑150 (MQ=255)

                                                                               gCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAg                                                                        >  2:892626/1‑150 (MQ=255)

                                                                                ccGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGt                                                                       >  1:1304189/1‑150 (MQ=255)

                                                                                 cGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGCGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTc                                                                      <  1:28764/150‑1 (MQ=255)

                                                                                       tAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGcc                                                                >  1:1181013/1‑150 (MQ=255)

                                                                                         aTTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTg                                                              >  1:889993/1‑150 (MQ=255)

                                                                                           ttgattgaTGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGAt                                                            >  2:297052/1‑150 (MQ=255)

                                                                                            tgattgatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGAtt                                                           <  1:1329609/150‑1 (MQ=255)

                                                                                             gattgatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTa                                                          >  2:1111491/1‑150 (MQ=255)

                                                                                                 gatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACg                                                      >  2:1216855/1‑150 (MQ=255)

                                                                                                   tGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGcc                                                    <  2:2214910/150‑1 (MQ=255)

                                                                                                     cGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTg                                                  >  2:104951/1‑150 (MQ=255)

                                                                                                     cGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTg                                                  >  2:1978224/1‑150 (MQ=255)

                                                                                                      gTTACAAGTAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGGTGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTTCTGTAGTCCAGGCCTGATTAAACGCCTGa                                                 <  2:2084354/150‑1 (MQ=255)

                                                                                                        tACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATg                                               >  1:440280/1‑150 (MQ=255)

                                                                                                         aCAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGc                                              <  1:575788/150‑1 (MQ=255)

                                                                                                            tggAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCAGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCtgt                                           >  2:2213935/2‑150 (MQ=255)

                                                                                                            aGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCtgt                                           >  2:191687/1‑150 (MQ=255)

                                                                                                            aGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCtgt                                           >  2:2222615/1‑150 (MQ=255)

                                                                                                             ggAAAGGTTGAGAAGAACTGTGTGAATCAGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCtgtg                                          >  1:2127472/1‑150 (MQ=255)

                                                                                                                  ggTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGt                                     <  1:741565/150‑1 (MQ=255)

                                                                                                                   gTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTa                                    >  1:524136/1‑150 (MQ=255)

                                                                                                                    ttGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTaa                                   >  1:1972203/1‑150 (MQ=255)

                                                                                                                      gagaAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATg                                 >  1:2145460/1‑150 (MQ=255)

                                                                                                                       agaagaACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATgg                                <  1:711026/150‑1 (MQ=255)

                                                                                                                        gaagaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgc                               >  1:2169962/1‑150 (MQ=255)

                                                                                                                        gaagaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgc                               >  2:2453895/1‑150 (MQ=255)

                                                                                                                         aagaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgcg                              <  1:724225/150‑1 (MQ=255)

                                                                                                                          agaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgcgc                             <  2:1228987/150‑1 (MQ=255)

                                                                                                                           gaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCa                            >  2:2095729/1‑150 (MQ=255)

                                                                                                                                gtgtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAg                       <  1:896523/150‑1 (MQ=255)

                                                                                                                                 tgtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGt                      >  2:455108/1‑150 (MQ=255)

                                                                                                                                    gAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTg                   >  2:929569/1‑150 (MQ=255)

                                                                                                                                     aaTCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGt                  <  2:1930716/150‑1 (MQ=255)

                                                                                                                                       tCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTta                <  1:463308/150‑1 (MQ=255)

                                                                                                                                           gcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATAtt            >  1:2277478/4‑150 (MQ=255)

                                                                                                                                           gcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATAtt            >  1:1570916/4‑150 (MQ=255)

                                                                                                                                            cagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATAtta           >  2:217063/3‑149 (MQ=255)

                                                                                                                                            cagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTc           >  2:1212006/3‑150 (MQ=255)

                                                                                                                                                 gcgGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTg      <  1:1899520/150‑1 (MQ=255)

                                                                                                                                                 gcgGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTg      >  1:1103844/1‑150 (MQ=255)

                                                                                                                                                   ggACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGcc    <  2:377308/150‑1 (MQ=255)

                                                                                                                                                     aCTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctg  >  2:1727987/1‑150 (MQ=255)

GCTCTCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTG  >  NC_000913/3718131‑3718429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`