BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,718,284	A→G	6.4%	325 (TAA→TAG)	ghrB →	glyoxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,718,284	0	A	G	6.4%	479.0 / 15.6	157	325 (TAA→TAG)	ghrB	glyoxylate reductase
Reads supporting (aligned to +/- strand): ref base A (76/71); new base G (7/3); total (83/74)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 162 total aligned reads displayed.

TCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGT  >  NC_000913/3718135‑3718433

 cAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAg                                                                                                                                                      >  1:653124/1‑150 (MQ=255)

  aaTGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGc                                                                                                                                                     <  1:491251/150‑1 (MQ=255)

  aaTGGCCAACGTCGTCGCAGTACCACATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGc                                                                                                                                                     >  2:352482/1‑150 (MQ=255)

        cAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGAc                                                                                                                                               <  2:2097892/150‑1 (MQ=255)

             tcgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGt                                                                                                                                          <  2:1756838/150‑1 (MQ=255)

             tcgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGt                                                                                                                                          >  1:1930518/1‑150 (MQ=255)

              cgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTa                                                                                                                                         >  2:724225/1‑150 (MQ=255)

              cgtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTa                                                                                                                                         <  2:1901402/150‑1 (MQ=255)

               gtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTag                                                                                                                                        <  1:2155864/150‑1 (MQ=255)

               gtcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTag                                                                                                                                        <  1:293142/150‑1 (MQ=255)

                tcgCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTaga                                                                                                                                       <  2:1258536/150‑1 (MQ=255)

                       aCCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGc                                                                                                                                >  2:1332568/1‑150 (MQ=255)

                       aCCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGc                                                                                                                                >  2:1200333/1‑150 (MQ=255)

                         cGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGccc                                                                                                                              <  2:2468998/150‑1 (MQ=255)

                          gCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCg                                                                                                                             <  2:1053258/150‑1 (MQ=255)

                               ttGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAAt                                                                                                                        >  1:1515294/1‑150 (MQ=255)

                                 ggATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCg                                                                                                                      <  2:2351482/150‑1 (MQ=255)

                                  gATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGAt                                                                                                                           >  2:514484/1‑144 (MQ=255)

                                  gATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGc                                                                                                                     >  1:1115118/1‑150 (MQ=255)

                                      tGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCgg                                                                                                                 >  1:318419/1‑150 (MQ=255)

                                             cATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTa                                                                                                          >  1:926283/1‑150 (MQ=255)

                                              aTGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTAc                                                                                                         <  1:1371268/150‑1 (MQ=255)

                                                gagaCGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTActc                                                                                                       >  1:2450091/1‑150 (MQ=255)

                                                   aCGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCttt                                                                                                    <  2:1515294/150‑1 (MQ=255)

                                                    cgcgTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTa                                                                                                   >  1:1304475/1‑150 (MQ=255)

                                                     gcgTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTAt                                                                                                  <  1:875812/150‑1 (MQ=255)

                                                       gTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTg                                                                                                >  1:221828/1‑150 (MQ=255)

                                                        ttATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTgg                                                                                               <  2:320260/150‑1 (MQ=255)

                                                          atggcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGt                                                                                             <  2:1822163/150‑1 (MQ=255)

                                                            ggcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTg                                                                                           >  1:660450/1‑150 (MQ=255)

                                                             gcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGc                                                                                          <  1:2210987/150‑1 (MQ=255)

                                                             gcatggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGc                                                                                          <  2:1930518/150‑1 (MQ=255)

                                                               atggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAg                                                                                        <  2:372759/150‑1 (MQ=255)

                                                               atggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAg                                                                                        <  2:1355334/150‑1 (MQ=255)

                                                               atggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAg                                                                                        <  2:269645/150‑1 (MQ=255)

                                                                tggcCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGt                                                                                       <  1:352482/150‑1 (MQ=255)

                                                                  gccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTaa                                                                                     >  1:1956955/1‑150 (MQ=255)

                                                                  gccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTaa                                                                                     >  2:981919/1‑150 (MQ=255)

                                                                   ccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAc                                                                                    <  1:1394076/150‑1 (MQ=255)

                                                                   ccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAc                                                                                    <  2:802641/150‑1 (MQ=255)

                                                                   ccgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAc                                                                                    <  1:782507/150‑1 (MQ=255)

                                                                    cgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAct                                                                                   <  2:922886/150‑1 (MQ=255)

                                                                    cgccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAAct                                                                                   >  1:1055959/1‑150 (MQ=255)

                                                                      ccTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCAGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAActgc                                                                                 >  1:1114708/1‑150 (MQ=255)

                                                                         gtgCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGt                                                                              <  2:188369/150‑1 (MQ=255)

                                                                         gagCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGt                                                                              >  2:741565/3‑150 (MQ=255)

                                                                            ccGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGt                                                                           >  1:1304189/1‑150 (MQ=255)

                                                                                 gATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAgg                                                                      >  2:463308/1‑150 (MQ=255)

                                                                                   tAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGcc                                                                    >  1:1181013/1‑150 (MQ=255)

                                                                                     aTTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTg                                                                  >  1:889993/1‑150 (MQ=255)

                                                                                     aTTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCAGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCAGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTg                                                                  >  1:2084354/1‑150 (MQ=255)

                                                                                        tgattgatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGAtt                                                               <  1:1329609/150‑1 (MQ=255)

                                                                                         gattgatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTa                                                              >  2:59747/1‑150 (MQ=255)

                                                                                         gattgatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTa                                                              >  2:1111491/1‑150 (MQ=255)

                                                                                         gattgatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTa                                                              <  2:1579070/150‑1 (MQ=255)

                                                                                             gatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACg                                                          >  2:1216855/1‑150 (MQ=255)

                                                                                             gatGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACg                                                          >  1:2025768/1‑150 (MQ=255)

                                                                                               tGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGcc                                                        >  1:213506/1‑150 (MQ=255)

                                                                                               tGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGcc                                                        <  2:2214910/150‑1 (MQ=255)

                                                                                                 cGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTg                                                      >  2:1978224/1‑150 (MQ=255)

                                                                                                 cGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTg                                                      >  2:104951/1‑150 (MQ=255)

                                                                                                  gTTACAAGTAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGGTGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTTCTGTAGTCCAGGCCTGATTAAACGCCTGa                                                     <  2:2084354/150‑1 (MQ=255)

                                                                                                  gTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGa                                                     >  1:1684448/1‑150 (MQ=255)

                                                                                                    tACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATg                                                   >  1:440280/1‑150 (MQ=255)

                                                                                                        aGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCtgt                                               >  2:191687/1‑150 (MQ=255)

                                                                                                         ggAAAGGTTGAGAAGAACTGTGTGAATCAGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCtgtg                                              >  1:2127472/1‑150 (MQ=255)

                                                                                                             aGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCgg                                          <  2:1477257/150‑1 (MQ=255)

                                                                                                              ggTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGt                                         >  2:768769/1‑150 (MQ=255)

                                                                                                              ggTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGt                                         <  1:741565/150‑1 (MQ=255)

                                                                                                               gTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTa                                        >  1:524136/1‑150 (MQ=255)

                                                                                                                ttGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTaa                                       >  1:1972203/1‑150 (MQ=255)

                                                                                                                 tGAGACGAACTGTGTGAATCCGCACGTCGCCGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCGCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAAt                                      <  2:2346426/150‑1 (MQ=255)

                                                                                                                 tGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAAt                                      >  1:1401365/1‑150 (MQ=255)

                                                                                                                   agaagaACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATgg                                    <  1:711026/150‑1 (MQ=255)

                                                                                                                    gaagaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgc                                   >  1:2169962/1‑150 (MQ=255)

                                                                                                                     aagaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgcg                                  <  1:724225/150‑1 (MQ=255)

                                                                                                                      agaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgcgc                                 <  2:221828/150‑1 (MQ=255)

                                                                                                                      agaaCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGgcgc                                 <  2:1228987/150‑1 (MQ=255)

                                                                                                                          cTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATc                             >  1:42258/1‑150 (MQ=255)

                                                                                                                            gtgtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAg                           <  1:1332568/150‑1 (MQ=255)

                                                                                                                            gtgtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAg                           <  1:896523/150‑1 (MQ=255)

                                                                                                                            gtgtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAg                           >  1:197134/1‑150 (MQ=255)

                                                                                                                              gtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGtt                         >  2:2091044/1‑150 (MQ=255)

                                                                                                                                gAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTg                       >  2:929569/1‑150 (MQ=255)

                                                                                                                                   tCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTta                    <  1:463308/150‑1 (MQ=255)

                                                                                                                                   ctacgcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTta                    >  2:874189/8‑150 (MQ=255)

                                                                                                                                      gCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTatat                 <  2:302966/150‑1 (MQ=255)

                                                                                                                                      cgcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTatat                 >  2:2189463/5‑150 (MQ=255)

                                                                                                                                      cgcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTatat                 >  2:215415/5‑150 (MQ=255)

                                                                                                                                       gcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATAtt                >  1:1570916/4‑150 (MQ=255)

                                                                                                                                       gcagTCGCGGCTTAGTCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATAtt                >  1:2277478/4‑150 (MQ=255)

                                                                                                                                            cgcgGAATAAGCCGCGAATGAGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCATGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACtt           >  2:1888177/1‑150 (MQ=255)

                                                                                                                                             gcgGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTg          >  1:1103844/1‑150 (MQ=255)

                                                                                                                                               ggACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGcc        <  2:2412424/150‑1 (MQ=255)

                                                                                                                                               ggACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGcc        <  2:377308/150‑1 (MQ=255)

                                                                                                                                                 aCTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctg      >  2:1727987/1‑150 (MQ=255)

                                                                                                                                                    aaGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctgctg   >  2:440246/1‑150 (MQ=255)

                                                                                                                                                    aaGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctgctg   <  1:2162213/150‑1 (MQ=255)

                                                                                                                                                     aGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctgctgg  >  1:2289241/1‑149 (MQ=255)

                                                                                                                                                     aGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCtgt  >  1:626536/1‑150 (MQ=255)

TCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGT  >  NC_000913/3718135‑3718433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`