BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,729,408	C→G	9.2%	intergenic (‑37/+35)	xylB ← / ← xylA	xylulokinase/xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,408	0	C	G	9.2%	575.3 / 37.6	182	intergenic (‑37/+35)	xylB/xylA	xylulokinase/xylose isomerase
Reads supporting (aligned to +/- strand): ref base C (84/81); new base G (5/12); total (89/93)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 185 total aligned reads displayed.

GGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTG  >  NC_000913/3729259‑3729555

 gATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTAcc                                                                                                                                                    <  1:1899010/150‑1 (MQ=255)

     cGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATa                                                                                                                                                >  1:1082005/1‑150 (MQ=255)

      gggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATaa                                                                                                                                               >  2:427114/1‑150 (MQ=255)

           cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCggg                                                                                                                                          <  1:2222669/150‑1 (MQ=255)

           cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGCTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCggg                                                                                                                                          >  1:2202106/1‑150 (MQ=255)

            gAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATACCTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGG‑TATCGGTAGCGAT‑ACCGGGc                                                                                                                                         <  1:1193246/150‑1 (MQ=255)

              aaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAACTGCCC‑GGTTATCGGTAGCGAT‑ACCGGGcat                                                                                                                                       <  1:1624084/150‑3 (MQ=255)

              aaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCa                                                                                                                                       >  2:1371858/1‑150 (MQ=255)

               aCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCaa                                                                                                                                      <  2:645662/150‑1 (MQ=255)

                 ggTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GGTTATCGGTAGCGAT‑ACCGGGcatttt                                                                                                                                    <  1:2176800/150‑6 (MQ=255)

                 ggTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACg                                                                                                                                    >  1:1337934/1‑150 (MQ=255)

                   tCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GGTTATCGGTAGCGAT‑ACCGGGcatttttt                                                                                                                                  <  2:1441311/150‑8 (MQ=255)

                      gCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTg                                                                                                                               >  1:374837/1‑150 (MQ=255)

                      gCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTg                                                                                                                               <  1:839230/150‑1 (MQ=255)

                       cTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGc                                                                                                                              <  2:40782/150‑1 (MQ=255)

                       cTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGc                                                                                                                              <  1:2167515/150‑1 (MQ=255)

                       cTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGc                                                                                                                              >  1:985208/1‑150 (MQ=255)

                         tttCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGcac                                                                                                                            <  2:1290985/150‑1 (MQ=255)

                            ccGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGt                                                                                                                         <  2:1771665/150‑1 (MQ=255)

                             cGTTTGCGCAGCAACCCCCACACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAgat                                                                                                                        >  2:1606912/1‑148 (MQ=255)

                                ttGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGc                                                                                                                     >  2:63143/1‑150 (MQ=255)

                                 tGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGcc                                                                                                                    >  2:617559/1‑150 (MQ=255)

                                    gcagcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGtt                                                                                                                 >  1:2419277/1‑150 (MQ=255)

                                     cagcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTa                                                                                                                <  1:1642218/150‑1 (MQ=255)

                                       gcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                                                                              <  1:506172/150‑1 (MQ=255)

                                       gcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                                                                              <  1:515053/150‑1 (MQ=255)

                                       gcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                                                                              >  2:199386/1‑150 (MQ=255)

                                        caACCACATCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATATATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAttt                                                                                                             >  2:2314196/1‑150 (MQ=255)

                                          accaccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGt                                                                                                           >  2:2353044/1‑150 (MQ=255)

                                          accaccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGt                                                                                                           >  2:2361629/1‑150 (MQ=255)

                                           ccaccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTc                                                                                                          <  1:2229884/150‑1 (MQ=255)

                                           ccaccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTc                                                                                                          <  2:2156396/150‑1 (MQ=255)

                                             accTCACCCTGCTCGTTTAGCTAAATTACTTTTAGTCGTTAGGTGCCAAGATCTATCCCTTTATACATATCGATCGTTCCTTAAAAAAATTCCC‑GG‑TATCGCTAGCGATAACCGGGCCAACGGAGTGCTCAGTTTGCCGTTATTTGTCGa                                                                                                        <  2:1034481/150‑1 (MQ=255)

                                             accTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGa                                                                                                        >  2:2177005/1‑150 (MQ=255)

                                                 cACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                                                                                                    >  2:773879/1‑150 (MQ=255)

                                                 cACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                                                                                                    <  1:322211/150‑1 (MQ=255)

                                                  aCCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGGCGAACAGa                                                                                                   <  2:1790001/150‑1 (MQ=255)

                                                   cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGAt                                                                                                  >  2:1159582/1‑150 (MQ=255)

                                                   cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGAt                                                                                                  >  2:2038064/1‑150 (MQ=255)

                                                   cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGAt                                                                                                  >  1:807244/1‑150 (MQ=255)

                                                       gCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATg                                                                                              <  1:1076819/150‑1 (MQ=255)

                                                          cGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                                                                           <  2:2291922/150‑1 (MQ=255)

                                                             tGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAc                                                                                        <  1:1938244/150‑1 (MQ=255)

                                                              gAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                                                                       >  1:1164911/1‑150 (MQ=255)

                                                              gAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                                                                       >  1:259515/1‑150 (MQ=255)

                                                                gCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAg                                                                                     >  1:408128/1‑150 (MQ=255)

                                                                    aaTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGGCC‑GG‑TATCGCTACAGATAACCGGGCCAACTGACTGCACAGTTAGCCGTTATTTGTAGAACAGATAATGGTTTACCAGAttt                                                                                 <  2:1643271/150‑1 (MQ=255)

                                                                       aaCTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTcc                                                                              <  1:2037309/150‑1 (MQ=255)

                                                                         cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                                                                            >  1:1073771/1‑150 (MQ=255)

                                                                         cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                                                                            <  2:122741/150‑1 (MQ=255)

                                                                          ttttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgt                                                                           <  2:1424920/150‑1 (MQ=255)

                                                                           attACGCCCGAGGTGCCAAGATCTATCCCGATATACATATTGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgtt                                                                          >  1:35190/2‑150 (MQ=255)

                                                                            ttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttg                                                                         <  1:773879/150‑1 (MQ=255)

                                                                               cGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTc                                                                      >  2:284546/1‑150 (MQ=255)

                                                                                   cGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATGACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTgg                                                                  <  1:440242/150‑1 (MQ=255)

                                                                                   cGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTCCCAGTTGTTCCTgg                                                                  <  2:1673602/150‑1 (MQ=255)

                                                                                     aGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCg                                                                <  2:1200720/150‑1 (MQ=255)

                                                                                       gttcaaaGATCTATCCCGATATACATATCGATCTTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTATCCGTTATTTGTCGAACAGATAATGGTTTACCATATTTTCCAGTTGTTCCTGGCGAc                                                              <  2:1925101/145‑1 (MQ=255)

                                                                                        tGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCT‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGAcc                                                             <  1:1302238/150‑1 (MQ=255)

                                                                                        tGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGAcc                                                             <  1:1542697/150‑1 (MQ=255)

                                                                                         gCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCCGATTTTCCAGTTGTTCCTGGCGACCa                                                            >  2:1726026/1‑150 (MQ=255)

                                                                                         gCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCa                                                            >  2:269121/1‑150 (MQ=255)

                                                                                         gCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGAGACCa                                                            >  1:1238818/1‑150 (MQ=255)

                                                                                             aGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCActct                                                        <  1:286734/150‑1 (MQ=255)

                                                                                                tCTATCGCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGTTAATGGTTTAGCATATTTTCCAGTTGTTCCTGGCGACCACTCTGAt                                                     <  2:711926/150‑1 (MQ=255)

                                                                                                 cTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATTACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATg                                                    <  1:2291134/150‑1 (MQ=255)

                                                                                                 cTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATg                                                    <  1:708028/150‑1 (MQ=255)

                                                                                                  tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGc                                                   >  1:2379662/1‑150 (MQ=255)

                                                                                                     cccGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCAcc                                                >  2:2382446/1‑150 (MQ=255)

                                                                                                      ccGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCg                                               >  2:1965672/1‑150 (MQ=255)

                                                                                                         atatACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgag                                            <  2:895136/150‑1 (MQ=255)

                                                                                                         atatACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgag                                            <  1:2314196/150‑1 (MQ=255)

                                                                                                         atatACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgag                                            >  2:1024364/1‑150 (MQ=255)

                                                                                                           ataCATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGAc                                          <  2:1337934/150‑1 (MQ=255)

                                                                                                            taCATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACa                                         <  2:351628/150‑1 (MQ=255)

                                                                                                             aCATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaa                                        >  1:1866706/1‑150 (MQ=255)

                                                                                                             aCATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaa                                        >  1:1426873/1‑150 (MQ=255)

                                                                                                              cATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACAAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                       >  1:884792/1‑150 (MQ=255)

                                                                                                               atatCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAat                                      <  2:2193573/150‑1 (MQ=255)

                                                                                                                tatCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatg                                     >  2:1818260/1‑150 (MQ=255)

                                                                                                                 atcgatcgTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatga                                    >  1:423438/1‑150 (MQ=255)

                                                                                                                    gatcgTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGt                                 >  2:1281148/1‑150 (MQ=255)

                                                                                                                           ccTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg                          >  1:1834534/1‑150 (MQ=255)

                                                                                                                           ccTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg                          <  1:665223/150‑1 (MQ=255)

                                                                                                                            cTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGc                         >  1:2023107/1‑150 (MQ=255)

                                                                                                                            cTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGc                         >  1:494546/1‑150 (MQ=255)

                                                                                                                                aaaaaaTGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCatat                     >  1:1766896/1‑150 (MQ=255)

                                                                                                                                   aaaTGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTg                  <  2:985208/150‑1 (MQ=255)

                                                                                                                                   aaaTGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTg                  <  2:1364160/150‑1 (MQ=255)

                                                                                                                                        ccc‑GGTTATCGGTAGCGAT‑ACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa             >  1:2132383/1‑150 (MQ=255)

                                                                                                                                        ccc‑GGTTATCGGTAGCGAT‑ACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCAACGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa             >  1:1896996/1‑150 (MQ=255)

                                                                                                                                        ccc‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa             >  1:920262/1‑150 (MQ=255)

                                                                                                                                            gg‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATc          <  1:427114/150‑1 (MQ=255)

                                                                                                                                               tATCGCTACCGATAACCGGGCCAACTGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTg        <  2:1655079/150‑1 (MQ=255)

                                                                                                                                               tATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTg        <  1:1970/150‑1 (MQ=255)

                                                                                                                                                 tCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGcc      <  2:321587/150‑1 (MQ=255)

                                                                                                                                                 tCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGcc      <  1:2353044/150‑1 (MQ=255)

                                                                                                                                                   ggtACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAg    <  1:1850443/148‑1 (MQ=255)

                                                                                                                                                   gCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAg    >  2:2475466/1‑150 (MQ=255)

                                                                                                                                                     tACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTg  <  2:2242751/150‑1 (MQ=255)

GGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCC‑GG‑TATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTG  >  NC_000913/3729259‑3729555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`