BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,809,681	T→A	6.7%	V381D (GTT→GAT) ‡	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,681	0	T	A	6.7%	364.8 / 12.1	129	V381D (GTT→GAT) ‡	waaA	KDO transferase
Reads supporting (aligned to +/- strand): ref base T (65/55); new base A (3/6); total (68/61)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

Only 100 of 131 total aligned reads displayed.

TGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCA  >  NC_000913/3809532‑3809829

tggtggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGt                                                                                                                                                      >  2:927808/1‑150 (MQ=255)

tggtggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGt                                                                                                                                                      >  1:1578504/1‑150 (MQ=255)

   tggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTAACCGATGCCACTACGCTTGCAAAAGAGGTTTc                                                                                                                                                   >  1:1038813/1‑150 (MQ=255)

    gggCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTcc                                                                                                                                                  <  1:361687/150‑1 (MQ=255)

         tAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCttt                                                                                                                                             <  1:1936550/150‑1 (MQ=255)

           aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATTCCACTACGCTTGCAAAAGAGGTTTCCTCTTTAc                                                                                                                                           >  2:2469745/1‑150 (MQ=255)

           aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTAc                                                                                                                                           <  2:1261051/150‑1 (MQ=255)

           aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTAc                                                                                                                                           <  1:790496/150‑1 (MQ=255)

           aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttg                                                                                                                                           <  2:2043207/150‑3 (MQ=255)

           aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttg                                                                                                                                           <  2:1414863/150‑3 (MQ=255)

               gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAc                                                                                                                                       >  1:999171/1‑150 (MQ=255)

               gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAc                                                                                                                                       >  2:2196190/1‑150 (MQ=255)

               gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAc                                                                                                                                       >  2:1425637/1‑150 (MQ=255)

               gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAc                                                                                                                                       <  1:330084/150‑1 (MQ=255)

               gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGATTCCTCTTTACTCAc                                                                                                                                       <  1:1045326/150‑1 (MQ=255)

               gCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttgcaag                                                                                                                                       <  1:1739641/150‑7 (MQ=255)

                cTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCATCTTTACTCAcc                                                                                                                                      >  2:683128/1‑150 (MQ=255)

                cTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttgcaagc                                                                                                                                      <  1:1254834/150‑8 (MQ=255)

                 tGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCg                                                                                                                                     <  2:2470008/150‑1 (MQ=255)

                  ggAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGa                                                                                                                                    >  1:1089462/1‑150 (MQ=255)

                  ggAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGa                                                                                                                                    >  2:1161172/1‑150 (MQ=255)

                       cTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCg                                                                                                                               >  2:917288/1‑150 (MQ=255)

                         gCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGAt                                                                                                                             >  2:1120325/1‑150 (MQ=255)

                           cGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACAATAAAGACATTTGCGCGCGGCTCGAGCAGGCAAGCGGGCTGATTACCGATACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTAGTCACCGACGCCGATTa                                                                                                                           >  1:1607727/1‑150 (MQ=255)

                             cacaCGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTAcc                                                                                                                         <  1:508531/150‑1 (MQ=255)

                              acacGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCg                                                                                                                        <  2:450134/150‑1 (MQ=255)

                                 cGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAg                                                                                                                     >  1:1509942/1‑150 (MQ=255)

                                 cGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAg                                                                                                                     >  2:1880423/1‑150 (MQ=255)

                                 cGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAg                                                                                                                     >  1:2251538/1‑150 (MQ=255)

                                 cGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAg                                                                                                                     <  1:1861/150‑1 (MQ=255)

                                       tCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTa                                                                                                               <  1:2175881/150‑1 (MQ=255)

                                        ccGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTAt                                                                                                              >  1:2313709/1‑150 (MQ=255)

                                        ccGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTAt                                                                                                              >  2:348311/1‑150 (MQ=255)

                                         cGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATg                                                                                                             >  1:1126952/1‑150 (MQ=255)

                                          ggTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATgg                                                                                                            >  1:1164051/1‑150 (MQ=255)

                                             aTTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCg                                                                                                         <  1:575709/150‑1 (MQ=255)

                                             aTTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCg                                                                                                         <  2:1779606/150‑1 (MQ=255)

                                              ttGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGt                                                                                                        <  2:2349377/150‑1 (MQ=255)

                                                    gggCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGcc                                                                                                  >  2:1599663/1‑150 (MQ=255)

                                                     ggCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCg                                                                                                 >  1:2363698/1‑150 (MQ=255)

                                                      gCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGt                                                                                                <  2:650170/150‑1 (MQ=255)

                                                       ccGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGtt                                                                                               <  2:833046/150‑1 (MQ=255)

                                                         gCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGa                                                                                             >  2:1772564/1‑150 (MQ=255)

                                                           aTACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCGTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAg                                                                                           <  2:187797/150‑1 (MQ=255)

                                                            tACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGt                                                                                          >  2:1309092/1‑150 (MQ=255)

                                                              cTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTAc                                                                                        >  2:1332335/1‑150 (MQ=255)

                                                                tttAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTg                                                                                      <  2:373571/150‑1 (MQ=255)

                                                                     cTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCa                                                                                 >  1:1443383/1‑150 (MQ=255)

                                                                      tttAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCaa                                                                                <  2:1126952/150‑1 (MQ=255)

                                                                        tAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCaaaa                                                                              <  1:1158649/150‑1 (MQ=255)

                                                                          aaGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAAcc                                                                            <  1:1327136/150‑1 (MQ=255)

                                                                          aaGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAAcc                                                                            >  1:239129/1‑150 (MQ=255)

                                                                            taCATTTGCGCGCTGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCac                                                                          <  1:1494864/149‑2 (MQ=255)

                                                                            gACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAg                                                                          <  2:1164051/150‑1 (MQ=255)

                                                                            gACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAg                                                                          <  2:267316/150‑1 (MQ=255)

                                                                                      cgcgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTAc                                                                >  2:1091333/1‑150 (MQ=255)

                                                                                      cgcgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAACAGGTTTCCTCTTTACTCACCGACGCCGATTACAGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTAc                                                                >  2:2339455/1‑150 (MQ=255)

                                                                                       gcgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACa                                                               <  1:1676319/150‑1 (MQ=255)

                                                                                          gCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCg                                                            >  1:1566864/1‑150 (MQ=255)

                                                                                           cTGGAGCAGGCAAGCGGTATGATTACCGTTACCGATGCCACTACGCTTGAAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGt                                                           >  2:896710/1‑150 (MQ=255)

                                                                                              gAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTg                                                        >  1:1052956/1‑150 (MQ=255)

                                                                                               aGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGTCGCGCTACAGCGtattc                                                       <  2:1509942/150‑5 (MQ=255)

                                                                                                 cAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCATAACCAGGGCGCGCTACAGCGTCTGCtt                                                     <  2:2363698/150‑1 (MQ=255)

                                                                                                  aGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCtta                                                    >  1:2487742/1‑149 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                   <  1:2196190/150‑1 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                   >  1:434093/1‑150 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                   <  1:63362/150‑1 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                   >  1:921022/1‑150 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                   >  1:18791/1‑150 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACACCGTCTGCTTCa                                                   >  2:652785/1‑150 (MQ=255)

                                                                                                     cAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                                 >  1:2284550/1‑150 (MQ=255)

                                                                                                     cAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                                 <  2:1885267/150‑1 (MQ=255)

                                                                                                      aaGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAct                                                >  1:18763/1‑150 (MQ=255)

                                                                                                         cGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAActgct                                             <  1:1772564/150‑1 (MQ=255)

                                                                                                         cGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAActgct                                             <  2:1382582/150‑1 (MQ=255)

                                                                                                          gggCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAActgctg                                            >  1:645225/1‑150 (MQ=255)

                                                                                                          gggCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTAAAGCGTCTGCTTCAActgctg                                            >  2:2353270/1‑150 (MQ=255)

                                                                                                           ggCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTgg                                           >  2:1253527/1‑150 (MQ=255)

                                                                                                              agATTACCGTAACAGATGCCACTACGCTTGCAAAAGAGGATTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCAGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTCGAAc                                        >  2:1493172/2‑150 (MQ=255)

                                                                                                                   accgttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTAc                                   >  2:1817114/1‑150 (MQ=255)

                                                                                                                      gttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTg                                >  2:2410113/1‑150 (MQ=255)

                                                                                                                         accgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCa                             >  1:2016100/1‑150 (MQ=255)

                                                                                                                          ccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCAc                            <  1:963913/150‑1 (MQ=255)

                                                                                                                                ccACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGaaaa                      >  1:1484086/1‑150 (MQ=255)

                                                                                                                                 cACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTTCCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCCAAAAc                     >  1:375068/1‑150 (MQ=255)

                                                                                                                                  aCTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACg                    <  1:1512576/150‑1 (MQ=255)

                                                                                                                                  aCTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTATATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACg                    >  2:307137/1‑150 (MQ=255)

                                                                                                                                       gCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTg               <  1:1419263/150‑1 (MQ=255)

                                                                                                                                        gtgagtaaaGAGGAAACCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCAGTCATCCCGTTGAAGAACTGTAACAAAACCAGGTCGCGCTAACCAGTCTGAGTCAACTGATCGACACTTACAGGACACAGAAAACGCATTGa              >  2:1079064/7‑150 (MQ=255)

                                                                                                                                         tgattaaaGAGGAAACCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCCTCTGCTTCAACTGCTGCAACCTTACCTGCCACCGAAAACGCATTGAg             >  2:2452230/6‑150 (MQ=255)

                                                                                                                                          tGCAAAAGAGGTTTCCTCTTTACTCACCGACGACGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGCAACCTTACCTGCCACCGAAAACGCATTGAgg            >  2:788036/1‑150 (MQ=255)

                                                                                                                                            cAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGgtt          >  2:57619/1‑150 (MQ=255)

                                                                                                                                            cAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGgtt          >  2:217504/1‑150 (MQ=255)

                                                                                                                                              aaaGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGgttgt        <  1:1425637/150‑1 (MQ=255)

                                                                                                                                                agagGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAAGGCATTGAGgttgttt      <  1:687800/150‑2 (MQ=255)

                                                                                                                                                agagGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTa      <  2:2219912/150‑1 (MQ=255)

                                                                                                                                                   ggTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGc   >  1:658339/1‑150 (MQ=255)

                                                                                                                                                   ggTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGc   <  1:1309092/150‑1 (MQ=255)

                                                                                                                                                   ggTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGc   >  1:524545/1‑150 (MQ=255)

                                                                                                                                                    gTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCa  >  2:14262/1‑150 (MQ=255)

TGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCA  >  NC_000913/3809532‑3809829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`