BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,899,866	T→A	5.1%	K238* (AAA→TAA) ‡	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,899,866	0	T	A	5.1%	510.9 / 11.5	156	K238* (AAA→TAA) ‡	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand): ref base T (68/80); new base A (5/3); total (73/83)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 160 total aligned reads displayed.

CCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAAC  >  NC_000913/3899719‑3900015

ccAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATAAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTAc                                                                                                                                                     >  2:1687263/1‑150 (MQ=255)

 cAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACg                                                                                                                                                    <  1:1554585/150‑1 (MQ=255)

 cAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACg                                                                                                                                                    >  2:1659692/1‑150 (MQ=255)

        tGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTcc                                                                                                                                             <  2:2220207/150‑1 (MQ=255)

        tGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTcc                                                                                                                                             <  1:1786564/150‑1 (MQ=255)

         ggTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCt                                                                                                                                            <  1:2333397/150‑1 (MQ=255)

         ggTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCt                                                                                                                                            >  2:1166647/1‑150 (MQ=255)

         ggTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCt                                                                                                                                            >  1:1186764/1‑150 (MQ=255)

          gTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTg                                                                                                                                           >  1:1959884/1‑150 (MQ=255)

          gTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACGTAAAGTCttttatc                                                                                                                                           <  1:917940/150‑5 (MQ=255)

             cccGCCTTGTGATAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAgg                                                                                                                                        <  2:1884669/150‑1 (MQ=255)

              ccGCCTTGTGAATGACCTGCCAGCGCGCGTCCATCGGCATCTTTACGGACATTAAAACTCTTGCTAATCAGCTTGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCTGATAAAAGACTTTACTTCTTTCCTGAGGc                                                                                                                                       <  2:1627649/150‑1 (MQ=255)

                 ccTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACg                                                                                                                                    >  1:1278218/1‑150 (MQ=255)

                 ccTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACg                                                                                                                                    <  1:605859/150‑1 (MQ=255)

                    tgtgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGaaa                                                                                                                                 <  2:1769536/150‑1 (MQ=255)

                     gtgAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAAt                                                                                                                                <  2:2070040/150‑1 (MQ=255)

                       gAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCt                                                                                                                              <  1:1245153/150‑1 (MQ=255)

                        aaaGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAACACTTTACGTCTTTCCTGAGGCACGAAATCtt                                                                                                                             <  1:1160023/150‑1 (MQ=255)

                          aGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCg                                                                                                                           <  1:719387/150‑1 (MQ=255)

                           gCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTCCGTCTTTCCTGAGGCACGAAATCTTCgg                                                                                                                          >  2:1565320/1‑150 (MQ=255)

                           gCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCgg                                                                                                                          <  1:1759221/150‑1 (MQ=255)

                            cccTGCCAGCGCGCGGCCATCGTCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCggg                                                                                                                         <  1:216777/150‑1 (MQ=255)

                            cccTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCTTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCggg                                                                                                                         <  1:1097574/150‑1 (MQ=255)

                            cccTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCggg                                                                                                                         <  1:1018150/150‑1 (MQ=255)

                            cccTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGATTGCTAATCAGCGGGATAATATGGTTCATCAGTTCGCGATCTGACGC‑TTTAGCATTCAGCGGATAAAAGACTTTAGGTCTTTCCGGAGGCACGAAATCTTCggg                                                                                                                         >  2:440999/1‑150 (MQ=255)

                             ccTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGa                                                                                                                        >  1:991878/1‑150 (MQ=255)

                              cTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaa                                                                                                                       >  1:1479741/1‑150 (MQ=255)

                                      gcgcgGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGcac                                                                                                               >  1:1298409/1‑148 (MQ=255)

                                           gCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGc                                                                                                          <  2:529275/150‑1 (MQ=255)

                                           gCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGc                                                                                                          >  2:989446/1‑150 (MQ=255)

                                           gCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGc                                                                                                          >  1:1607669/1‑150 (MQ=255)

                                            ccATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCa                                                                                                         <  1:1560814/150‑1 (MQ=255)

                                             cATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAGTAATGCCCTTCGCAt                                                                                                        >  1:1393837/1‑150 (MQ=255)

                                              aTCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATc                                                                                                       <  1:661898/150‑1 (MQ=255)

                                                     tCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCt                                                                                                <  1:1326317/150‑1 (MQ=255)

                                                      cTTTACGGACATTAAAACGCTTGCTAATTAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTg                                                                                               >  2:884619/1‑150 (MQ=255)

                                                         tACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTAt                                                                                            <  2:139688/150‑1 (MQ=255)

                                                              aCATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCgg                                                                                        >  2:2162778/1‑150 (MQ=255)

                                                              aCATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCggg                                                                                       <  2:1496233/150‑1 (MQ=255)

                                                                 ttAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATc                                                                                    >  2:2125653/1‑150 (MQ=255)

                                                                 ttAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATc                                                                                    >  2:1521022/1‑150 (MQ=255)

                                                                  tAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATca                                                                                   <  1:73219/150‑1 (MQ=255)

                                                                  tAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATca                                                                                   >  2:628961/1‑150 (MQ=255)

                                                                  tAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATca                                                                                   <  1:1655279/150‑1 (MQ=255)

                                                                   aaaaCGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATcac                                                                                  >  2:114564/1‑150 (MQ=255)

                                                                   aaaaCGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATcac                                                                                  <  1:371008/150‑1 (MQ=255)

                                                                    aaaCGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATcacc                                                                                 >  1:1874706/1‑150 (MQ=255)

                                                                    aaaCGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATcacc                                                                                 <  2:110419/150‑1 (MQ=255)

                                                                      aCGCTTGCTAATCAGCGGGTTAATATCGTTCATCAGTTCTCGATCTGCCGC‑TTTATCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATcaccac                                                                               <  2:1901160/150‑1 (MQ=255)

                                                                       cGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCaccacc                                                                              <  1:2434590/150‑1 (MQ=255)

                                                                         cTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAg                                                                            <  2:86460/150‑1 (MQ=255)

                                                                           tGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCa                                                                          >  1:1451607/1‑150 (MQ=255)

                                                                                   aGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTa                                                                  <  2:1607669/150‑1 (MQ=255)

                                                                                    gCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTaa                                                                 >  1:2118627/1‑150 (MQ=255)

                                                                                      gggATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAAtt                                                               <  2:1479741/150‑1 (MQ=255)

                                                                                        gATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAAtttt                                                             >  2:511967/1‑150 (MQ=255)

                                                                                          taataTCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTcc                                                           <  1:2457789/150‑1 (MQ=255)

                                                                                           aataTCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTccc                                                          <  2:1031135/150‑1 (MQ=255)

                                                                                           aataTCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTccc                                                          <  2:1268483/150‑1 (MQ=255)

                                                                                            atatCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCt                                                         <  1:1659692/150‑1 (MQ=255)

                                                                                            atatCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCt                                                         <  2:551045/150‑1 (MQ=255)

                                                                                            atatCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGGCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCt                                                         <  1:1054838/150‑1 (MQ=255)

                                                                                              atCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTc                                                       <  2:733632/150‑1 (MQ=255)

                                                                                                 gTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCagc                                                    <  1:2112701/150‑1 (MQ=255)

                                                                                                 gTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCagc                                                    >  1:338904/1‑150 (MQ=255)

                                                                                                      tcaGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCagcaagca                                               <  2:2052518/150‑1 (MQ=255)

                                                                                                       caGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAg                                              >  2:1461001/1‑150 (MQ=255)

                                                                                                        aGTTCGCGTTCTGGCGC‑TTTAGCATTCTGCGGATAAAAGTCTTTACGTCTTTCCTGAGGCACGAATTCTTCGGGATTAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAgg                                             <  1:961771/150‑1 (MQ=255)

                                                                                                             gcgATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATc                                        <  1:1986724/150‑1 (MQ=255)

                                                                                                             gcgATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATc                                        <  2:2118627/150‑1 (MQ=255)

                                                                                                              cgATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATcc                                       >  2:1321380/1‑150 (MQ=255)

                                                                                                               gATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCa                                      >  2:2287108/1‑150 (MQ=255)

                                                                                                                aTCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCAt                                     <  2:1959884/150‑1 (MQ=255)

                                                                                                                  cTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGa                                   >  2:727012/1‑150 (MQ=255)

                                                                                                                   tGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGAt                                  >  1:310516/1‑150 (MQ=255)

                                                                                                                    gCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGAtt                                 >  1:1251824/1‑150 (MQ=255)

                                                                                                                    gCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGAtt                                 >  2:2168602/1‑150 (MQ=255)

                                                                                                                            aaGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCTGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCAATGATTTGCGGGa                          >  2:2372118/2‑150 (MQ=255)

                                                                                                                              gCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATa                        <  1:511967/150‑1 (MQ=255)

                                                                                                                               cATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATAc                       <  2:1286973/150‑1 (MQ=255)

                                                                                                                                aTTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACg                      >  1:838926/1‑150 (MQ=255)

                                                                                                                                  tCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGcc                    <  1:1379081/150‑1 (MQ=255)

                                                                                                                                  tCAGCGGACAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGcc                    <  2:249800/150‑1 (MQ=255)

                                                                                                                                      ctcaggaaaGACGTAAAGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTg                >  2:1745530/7‑150 (MQ=255)

                                                                                                                                      cGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTg                >  1:2159748/1‑150 (MQ=255)

                                                                                                                                       tcaggaaaGACGTAAAGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTgg               >  1:1325589/6‑149 (MQ=255)

                                                                                                                                         aggaaaGACGTAAAGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATc             >  1:2105008/4‑150 (MQ=255)

                                                                                                                                         aTAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATc             <  1:1865659/150‑1 (MQ=255)

                                                                                                                                          tAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCg            >  2:1572635/1‑150 (MQ=255)

                                                                                                                                          tAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCg            >  1:602381/1‑150 (MQ=255)

                                                                                                                                           aaaaGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGa           >  1:439417/1‑150 (MQ=255)

                                                                                                                                            aaaGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGAt          >  1:1312592/1‑150 (MQ=255)

                                                                                                                                              aGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAg        >  2:1607328/1‑150 (MQ=255)

                                                                                                                                               gACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGc       >  2:271383/1‑150 (MQ=255)

                                                                                                                                                 cTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTTTATCCGGGATCAAAACCATCATAGGTATAATTTTCCCTTCAGCAAGCAAGTTATCCATGATTAGCGGGATAAGCCCCTGATCGATAGCgg     >  1:1591060/1‑150 (MQ=255)

                                                                                                                                                 cTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCgg     <  2:1369539/150‑1 (MQ=255)

                                                                                                                                                   ttACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGaa   >  2:2174967/1‑150 (MQ=255)

                                                                                                                                                   ttACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGaa   <  2:1186764/150‑1 (MQ=255)

                                                                                                                                                    tACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAAc  >  2:563676/1‑150 (MQ=255)

                                                                                                                                                    tACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAAc  >  1:1420638/1‑150 (MQ=255)

CCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGC‑TTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAAC  >  NC_000913/3899719‑3900015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`