Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,072,817 A→C 6.6% L252R (CTG→CGG)  yihU ← 3‑sulfolactaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,072,8170AC 6.6% 446.3 / 18.4 148L252R (CTG→CGG) yihU3‑sulfolactaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (75/63);  new base C (5/5);  total (80/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCTCATCACTTTGACG  >  NC_000913/4072668‑4072966
                                                                                                                                                     |                                                                                                                                                     
     cGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTGCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCTGCATGTGCCAATGCCGCTgg                                                                                                                                                  <  2:53522/150‑1 (MQ=255)
      gTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCTGCATGTGCCAATGCCGCTggg                                                                                                                                                 <  2:1718680/150‑2 (MQ=255)
       ttACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCTGCATGTGCCAATGCCGCTgggg                                                                                                                                                <  2:2114389/150‑3 (MQ=255)
        tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGCCCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                                                                               >  1:2015557/1‑150 (MQ=255)
          cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCTGCATGTGCCAATGCCGCTgggggcc                                                                                                                                             <  1:2112912/150‑6 (MQ=255)
          cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGc                                                                                                                                             <  1:85977/150‑1 (MQ=255)
           aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                                                                            <  2:2335815/150‑1 (MQ=255)
             ttttACTTTGGCAGTCAACCCAGCACTGCCACGGACCTGTTCCAGAATGGCGGACCAGTACTGGCGACCCCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGACGCGGCCACCAGCTGCATTGGCACATGCCGCTGCTAGGCGAc                                                                                                                                          >  2:1970038/1‑150 (MQ=255)
              tttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                                                                         >  1:1648688/1‑150 (MQ=255)
               ttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACAt                                                                                                                                        <  1:2127550/150‑1 (MQ=255)
                 aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGCCCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGCCATCa                                                                                                                                      >  2:1238484/1‑150 (MQ=255)
                 aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGCCATCa                                                                                                                                      >  2:1400868/1‑150 (MQ=255)
                 aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                                                                      <  2:1885654/150‑1 (MQ=255)
                 aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                                                                      <  1:513192/150‑1 (MQ=255)
                 aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                                                                      >  2:1569370/1‑150 (MQ=255)
                 aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                                                                      >  2:525410/1‑150 (MQ=255)
                  cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCaa                                                                                                                                     >  2:1489823/1‑150 (MQ=255)
                   tttGGCAGTCCTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAg                                                                                                                                    <  1:2083908/150‑1 (MQ=255)
                   tttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAg                                                                                                                                    >  2:1421919/1‑150 (MQ=255)
                     tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                                                                  >  2:1482357/1‑150 (MQ=255)
                      ggCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGc                                                                                                                                 >  2:24964/1‑150 (MQ=255)
                       gCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCg                                                                                                                                >  1:767421/1‑150 (MQ=255)
                           tCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGc                                                                                                                            <  2:1868112/150‑1 (MQ=255)
                            cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                                                                           >  1:34937/1‑150 (MQ=255)
                              tCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCaa                                                                                                                         >  2:1105563/1‑150 (MQ=255)
                              tCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCaa                                                                                                                         >  2:1772637/1‑150 (MQ=255)
                              ccccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCaa                                                                                                                         <  1:576356/149‑1 (MQ=255)
                               cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                                                                        >  1:2164348/1‑150 (MQ=255)
                               cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                                                                        <  2:962356/150‑1 (MQ=255)
                                      cagACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTa                                                                                                                 >  1:394576/3‑150 (MQ=255)
                                      cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTa                                                                                                                 <  1:2137616/150‑1 (MQ=255)
                                        gACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATg                                                                                                               >  2:281725/1‑150 (MQ=255)
                                         acacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATgg                                                                                                              >  2:1460699/1‑150 (MQ=255)
                                          cacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATggg                                                                                                             >  1:1107970/1‑150 (MQ=255)
                                          cacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATggg                                                                                                             <  1:1614883/150‑1 (MQ=255)
                                                  tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGTGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcg                                                                                                     <  1:1428483/150‑1 (MQ=255)
                                                     tCCAGAATGGCGGACCAGTCCTGGCGACCGCTACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcgatc                                                                                                  <  2:2448431/150‑1 (MQ=255)
                                                     tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcgatc                                                                                                  >  1:673776/1‑150 (MQ=255)
                                                         gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGa                                                                                              >  1:1091043/1‑150 (MQ=255)
                                                            tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAgg                                                                                           <  2:1805687/150‑1 (MQ=255)
                                                             ggCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGc                                                                                          >  2:627154/1‑150 (MQ=255)
                                                             ggCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCACCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGc                                                                                          >  2:659206/1‑150 (MQ=255)
                                                                 gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGga                                                                                      >  1:1025083/1‑150 (MQ=255)
                                                                 gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGga                                                                                      >  2:379248/1‑150 (MQ=255)
                                                                   ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                                                                    >  2:1393650/1‑150 (MQ=255)
                                                                   ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                                                                    <  1:1909862/150‑1 (MQ=255)
                                                                     aGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaaa                                                                                  >  1:418704/1‑150 (MQ=255)
                                                                     aGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaaa                                                                                  >  2:1578125/1‑150 (MQ=255)
                                                                         cTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAta                                                                              >  2:2157147/1‑149 (MQ=255)
                                                                             cgaccgcgaccCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCg                                                                          >  1:107096/1‑150 (MQ=255)
                                                                                   cgaccCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                                                                    <  1:385727/150‑1 (MQ=255)
                                                                                   cgaccCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                                                                    <  1:350970/150‑1 (MQ=255)
                                                                                     accCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGCTCTCCGCTGAGGAc                                                                  >  1:1963430/1‑150 (MQ=255)
                                                                                         gCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttg                                                              >  1:954280/1‑150 (MQ=255)
                                                                                            gcgcgcgcCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTTGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttgttt                                                           <  2:1122146/150‑1 (MQ=255)
                                                                                               cgcgcCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTCGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGc                                                        <  1:316028/150‑1 (MQ=255)
                                                                                                gcgcCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGcc                                                       <  2:709896/150‑1 (MQ=255)
                                                                                                   ccTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAgg                                                    <  2:1626583/150‑1 (MQ=255)
                                                                                                     tGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa                                                  >  1:1380929/1‑150 (MQ=255)
                                                                                                      ggCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAg                                                 <  1:1043396/150‑1 (MQ=255)
                                                                                                        cTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAgtg                                               >  2:829147/1‑150 (MQ=255)
                                                                                                        cTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAgtg                                               >  1:424407/1‑150 (MQ=255)
                                                                                                         tataAACGTGCCGTGAGGCGGCCCCCAGCGGCATTGGGACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAgtgg                                              <  2:394576/150‑1 (MQ=255)
                                                                                                         tataAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAgtgg                                              >  1:1751892/1‑150 (MQ=255)
                                                                                                         tataAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAgtgg                                              <  1:1461197/150‑1 (MQ=255)
                                                                                                            aaaCCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGa                                           <  1:642919/150‑1 (MQ=255)
                                                                                                            aaaCCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGa                                           <  1:543304/150‑1 (MQ=255)
                                                                                                              aCCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACGTGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAg                                         >  1:1198134/1‑150 (MQ=255)
                                                                                                              aCCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAg                                         >  1:1628451/1‑150 (MQ=255)
                                                                                                                 tCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGa                                      >  2:172248/1‑150 (MQ=255)
                                                                                                                  cccGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGAc                                     <  1:1569370/150‑1 (MQ=255)
                                                                                                                   ccGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGAcc                                    >  1:1436861/1‑150 (MQ=255)
                                                                                                                   ccGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGAcc                                    <  1:2297614/150‑1 (MQ=255)
                                                                                                                   ccGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGACAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGAcc                                    <  2:2015557/150‑1 (MQ=255)
                                                                                                                        aGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTAc                               >  2:1233832/1‑150 (MQ=255)
                                                                                                                         ggcggcCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTAcc                              >  1:7661/1‑150 (MQ=255)
                                                                                                                             ccaaccAGCTGCATGTGCCAATGCCGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcg                          >  1:2486451/5‑150 (MQ=255)
                                                                                                                             ccaaccAGCTGCATGTGCCAATGCCGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcg                          >  1:2144692/5‑150 (MQ=255)
                                                                                                                             ccaaccAGCTGCATGTGCCAATGCCGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcg                          >  1:1996954/5‑150 (MQ=255)
                                                                                                                               ccccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCATATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcggc                        <  2:450517/150‑1 (MQ=255)
                                                                                                                                cccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcggcg                       <  2:749994/150‑1 (MQ=255)
                                                                                                                                cccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcggcg                       >  2:374588/1‑150 (MQ=255)
                                                                                                                                cccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcggcg                       <  2:767421/150‑1 (MQ=255)
                                                                                                                                cccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcggcg                       <  1:1961109/150‑1 (MQ=255)
                                                                                                                                cccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACaggcggcg                       >  1:247390/1‑150 (MQ=255)
                                                                                                                                 ccAGCGGCATTGGCACATGCAGCTGTTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAATATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCATTAAGTTTTTAAGTTACCTTTACcggcggcgg                      <  2:205675/150‑1 (MQ=255)
                                                                                                                                 ccAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACcggcggcgg                      <  2:1628451/150‑1 (MQ=255)
                                                                                                                                     cGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                                                                       >  2:290181/1‑97 (MQ=255)
                                                                                                                                     cGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                                                                       <  1:290181/97‑1 (MQ=255)
                                                                                                                                     cGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTAcc                  >  1:514047/1‑150 (MQ=255)
                                                                                                                                      ggCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGCTCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCg                 >  2:2315243/1‑150 (MQ=255)
                                                                                                                                       gCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGc                <  2:2074595/150‑1 (MQ=255)
                                                                                                                                          ttGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCtca             <  1:1416442/150‑1 (MQ=255)
                                                                                                                                           tGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCtcat            <  1:150350/150‑1 (MQ=255)
                                                                                                                                           tGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCtcat            <  2:766997/150‑1 (MQ=255)
                                                                                                                                              cacaTGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCTCATCAc         >  1:1494265/1‑150 (MQ=255)
                                                                                                                                              cacaTGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCTCATCAc         <  1:2224277/150‑1 (MQ=255)
                                                                                                                                              cacaTGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAACCTTATGGGCAAGATCGATCATGAACGCGGGAGAAAGATCTCCGCTGAGCACTTTCTTTGGCCAGGAAGTGCTCAAGTGACCTTTACCGGCGGCGGTACAGCTCATCAc         >  2:866958/1‑150 (MQ=255)
                                                                                                                                                   gCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCTCATCACTTTGa    <  1:1482357/150‑1 (MQ=255)
                                                                                                                                                     aGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCTCATCACTTTGACg  <  1:79770/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGGTGAAGTGACCTTTACCGGCGGCGGTACCGCTCATCACTTTGACG  >  NC_000913/4072668‑4072966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑