BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,095,940	G→A	6.9%	R15C (CGT→TGT)	rhaA ←	L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,095,940	0	G	A	6.9%	503.5 / 16.5	160	R15C (CGT→TGT)	rhaA	L‑rhamnose isomerase
Reads supporting (aligned to +/- strand): ref base G (72/77); new base A (5/6); total (77/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 164 total aligned reads displayed.

ATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTGAC  >  NC_000913/4095791‑4096089

aTTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACg                                                                                                                                                       <  2:2280528/150‑1 (MQ=255)

  tGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCt                                                                                                                                                     >  2:2183438/1‑150 (MQ=255)

   gCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTg                                                                                                                                                    >  2:1296632/1‑150 (MQ=255)

      tgtgGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGttt                                                                                                                                                 >  2:1214231/1‑150 (MQ=255)

        tgGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCg                                                                                                                                               >  2:1508442/1‑150 (MQ=255)

        tgGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCg                                                                                                                                               <  2:858343/150‑1 (MQ=255)

        tgGCCGGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCg                                                                                                                                               <  2:958846/150‑1 (MQ=255)

         ggCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGc                                                                                                                                              >  2:1795420/1‑150 (MQ=255)

           gcTGAATCCCCGCGGTCAGCGAACCTTCCGGTTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGATACGGGTAAAGGTTCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                                                                                                                            <  1:1052343/149‑3 (MQ=255)

           ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTa                                                                                                                                            <  2:1864554/150‑1 (MQ=255)

           ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTa                                                                                                                                            <  2:87339/150‑1 (MQ=255)

           ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTa                                                                                                                                            <  1:419557/150‑1 (MQ=255)

           ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                                                                                                                            <  1:2328374/150‑3 (MQ=255)

           ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                                                                                                                            <  1:255253/150‑3 (MQ=255)

           ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                                                                                                                            <  1:722355/150‑3 (MQ=255)

              gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCt                                                                                                                                         <  2:888851/150‑1 (MQ=255)

                aTCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTcc                                                                                                                                       <  2:2049911/150‑1 (MQ=255)

                aTCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTcc                                                                                                                                       >  2:1774039/1‑150 (MQ=255)

                  ccccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCa                                                                                                                                     >  2:742781/1‑150 (MQ=255)

                   cccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAg                                                                                                                                    >  1:2473725/1‑150 (MQ=255)

                    ccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAgg                                                                                                                                   <  1:22596/150‑1 (MQ=255)

                     cccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGc                                                                                                                                  >  1:1538278/1‑150 (MQ=255)

                      ccGTTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAAGATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCTATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGcc                                                                                                                                 <  2:391369/150‑1 (MQ=255)

                      ccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGcc                                                                                                                                 <  1:113384/150‑1 (MQ=255)

                       cGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTGTCGCTAGCTCCCAGGCCt                                                                                                                                <  1:981252/150‑1 (MQ=255)

                        ggTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTg                                                                                                                               <  1:2285098/150‑1 (MQ=255)

                        ggTCAGCGAACCTTCCGGGTTTTCAAAAACGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAAAGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTCTTTCGCTAGCTCCCAGGCCTg                                                                                                                               >  2:159958/1‑150 (MQ=255)

                             gCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCa                                                                                                                          >  2:1345489/1‑150 (MQ=255)

                              cGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAg                                                                                                                         <  2:1143902/150‑1 (MQ=255)

                              cGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAg                                                                                                                         >  2:2018309/1‑150 (MQ=255)

                              cGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAg                                                                                                                         >  1:1357771/1‑150 (MQ=255)

                               gatgtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGt                                                                                                                        >  2:1910167/6‑150 (MQ=255)

                               gAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGt                                                                                                                        >  2:1941375/1‑150 (MQ=255)

                                aaCCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGtt                                                                                                                       <  1:1831956/150‑1 (MQ=255)

                                 tgtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTg                                                                                                                      >  2:539668/4‑150 (MQ=255)

                                  gtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGa                                                                                                                     >  2:1729833/3‑150 (MQ=255)

                                     tCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTg                                                                                                                  >  2:26966/1‑150 (MQ=255)

                                       cGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGt                                                                                                                <  1:2204497/150‑1 (MQ=255)

                                       cGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGt                                                                                                                <  2:468818/150‑1 (MQ=255)

                                          gTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCAt                                                                                                             <  2:280903/150‑1 (MQ=255)

                                           ttttCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATg                                                                                                            >  2:2384787/1‑150 (MQ=255)

                                             ttCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcg                                                                                                          <  1:837956/150‑1 (MQ=255)

                                             ttCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCACGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcg                                                                                                          >  1:56267/1‑150 (MQ=255)

                                              tCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcgc                                                                                                         >  2:1651167/1‑150 (MQ=255)

                                              tCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcgc                                                                                                         >  1:184099/1‑150 (MQ=255)

                                                aaaaCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTGCAGTTGAGTGGTCATGCGCaa                                                                                                       >  2:408588/1‑150 (MQ=255)

                                                  aaCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAg                                                                                                     <  2:726502/150‑1 (MQ=255)

                                                  aaCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAg                                                                                                     <  2:2230837/150‑1 (MQ=255)

                                                          aCATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtg                                                                                             <  1:1011700/150‑1 (MQ=255)

                                                           catcatCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgt                                                                                            <  1:2049784/150‑1 (MQ=255)

                                                                  gCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCCCCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCgt                                                                                     >  1:1613188/1‑150 (MQ=255)

                                                                      tGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTag                                                                                 <  1:506118/150‑1 (MQ=255)

                                                                         cagcagTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGt                                                                              >  2:1988906/1‑150 (MQ=255)

                                                                         cagcagTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGt                                                                              >  2:1395644/1‑150 (MQ=255)

                                                                          agcagTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTg                                                                             >  2:608626/1‑150 (MQ=255)

                                                                                 cATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTgc                                                                      >  2:796582/1‑150 (MQ=255)

                                                                                  aTTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTgcg                                                                     <  1:1510892/150‑1 (MQ=255)

                                                                                  aTTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTgcg                                                                     >  1:513734/1‑150 (MQ=255)

                                                                                    tGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGcc                                                                   <  1:2206066/150‑1 (MQ=255)

                                                                                     gAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGccc                                                                  >  2:446080/1‑149 (MQ=255)

                                                                                     gAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCca                                                                  >  1:575703/1‑150 (MQ=255)

                                                                                           ggTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGt                                                            <  1:1917977/150‑1 (MQ=255)

                                                                                            gTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTg                                                           <  1:1443381/150‑1 (MQ=255)

                                                                                              aaaCGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTggg                                                         <  1:1774039/150‑1 (MQ=255)

                                                                                                 cGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCaa                                                      >  2:641721/1‑150 (MQ=255)

                                                                                                      aaGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCa                                                 <  2:955081/150‑1 (MQ=255)

                                                                                                      aaGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCACGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCa                                                 <  2:56267/150‑1 (MQ=255)

                                                                                                        gTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACt                                               >  2:330762/1‑150 (MQ=255)

                                                                                                         ttGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTg                                              <  2:2131599/150‑1 (MQ=255)

                                                                                                             cgcAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAg                                          <  2:1019000/150‑1 (MQ=255)

                                                                                                               cAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGa                                        <  1:1399173/150‑1 (MQ=255)

                                                                                                                aGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGAt                                       <  1:2041010/150‑1 (MQ=255)

                                                                                                                 gggcCTCCTCGTCATCTTTGCCCAGCTCCGCGAAACGCTGTTTCGCTAGGTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGAtt                                      <  1:2075840/148‑1 (MQ=255)

                                                                                                                    cctcctCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAgg                                   <  2:428099/150‑1 (MQ=255)

                                                                                                                    cctcctCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAgg                                   <  1:1214231/150‑1 (MQ=255)

                                                                                                                     ctcctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAggg                                  <  1:796582/150‑1 (MQ=255)

                                                                                                                       cctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTg                                                                             <  2:1090252/105‑1 (MQ=255)

                                                                                                                           gACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAg                            >  2:1830939/1‑150 (MQ=255)

                                                                                                                              aTCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAggtg                         <  2:184099/150‑1 (MQ=255)

                                                                                                                              aTCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAggtg                         >  2:666997/1‑150 (MQ=255)

                                                                                                                               tCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAggtgg                        <  1:1941375/150‑1 (MQ=255)

                                                                                                                                  aTCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCa                     <  1:1958650/150‑1 (MQ=255)

                                                                                                                                  aTCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCa                     >  1:1091216/1‑150 (MQ=255)

                                                                                                                                  aTCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCa                     <  1:380227/150‑1 (MQ=255)

                                                                                                                                    ccccACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGa                   >  2:1456486/1‑150 (MQ=255)

                                                                                                                                     cccACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGAt                  <  1:742781/150‑1 (MQ=255)

                                                                                                                                     cccACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGGAAAGGTGGTCAGAt                  >  1:168599/1‑150 (MQ=255)

                                                                                                                                      ccACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGAtt                 >  1:1856002/1‑150 (MQ=255)

                                                                                                                                         ccgccgCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTcgc              >  1:621512/1‑150 (MQ=255)

                                                                                                                                         agctagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTcgc              >  1:1764972/6‑150 (MQ=255)

                                                                                                                                          gctagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTcgcg             >  1:891808/5‑150 (MQ=255)

                                                                                                                                           ctagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCgg            >  1:932509/4‑150 (MQ=255)

                                                                                                                                           cccgcGAAACGCTGTTTCGCTAGCTCCCATGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCgg            <  2:1597355/149‑1 (MQ=255)

                                                                                                                                            tagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCggt           >  2:15560/3‑150 (MQ=255)

                                                                                                                                            ccgcGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCggt           <  2:575703/150‑1 (MQ=255)

                                                                                                                                             agcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTTGCggtg          >  2:991122/2‑150 (MQ=255)

                                                                                                                                                 aaaCGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGc      <  1:365420/150‑1 (MQ=255)

                                                                                                                                                    cGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTga   <  1:1702794/150‑1 (MQ=255)

                                                                                                                                                     gCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTgac  <  1:26966/150‑1 (MQ=255)

                                                                                                                                                     gCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTgac  <  1:2018309/150‑1 (MQ=255)

ATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTGAC  >  NC_000913/4095791‑4096089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`