Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 422,537 T→G 7.9% L7R (CTG→CGG)  malZ → maltodextrin glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,5370TG 7.9% 392.6 / 23.2 140L7R (CTG→CGG) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (55/73);  new base G (5/6);  total (61/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 140 total aligned reads displayed.

GAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTC  >  NC_000913/422389‑422683
                                                                                                                                                    |                                                                                                                                                  
 aaaTCCTGATCCTGCTATTTCGAAAGTTATCCGACCCCGTCCTCCTCCACCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGTCAAGTTGATCAGATTTTCATCACAGGGGTATTATGATGTTAAATGCATGGCACCTGc                                                                                                                                                  <  2:1583581/150‑1 (MQ=255)
 aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                                                                                                                                  <  1:1844111/150‑1 (MQ=255)
    tCCTGATCCGGCTATTCCGAAAGTTATCGGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAgg                                                                                                                                               >  2:941635/1‑150 (MQ=255)
    tCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCGCCCCCAAATAGCCTTCAGATGATGAGTGATGCTGGATGAGGCGAGGGGAAGGTGATCAGATTATCATAACAGGGGAATTATGATGTTAATTGAATGGCACCTGCCgg                                                                                                                                               >  2:2253043/1‑150 (MQ=255)
    tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTACAGAGGATGAGGGCAACTGCAGTAGGCTAGGGCAAGGTGGTCAGAGTTTCAGCACAGGGGAAGTATGATGTTAAATGGATGGCACATGCCgg                                                                                                                                               >  2:309517/1‑150 (MQ=255)
     ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGTAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                                                                                                                              <  1:1705136/150‑1 (MQ=255)
     ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCACCTCCCCCAAAAATACTAAAGATGATGAGTGATCCTGCATTAGGCTATGGTAAGGTGATAAGAATTTAATAAAAAGGGAATTATGATGTTAAATGAATTGAACCTGCCggg                                                                                                                                              >  2:1705136/1‑149 (MQ=255)
      cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCTCCTCCCCCAAATATACTTAAGATGATGAGTGATACTGAATTAGGCTATGGCAATGTGATAAGATTTTAATAACAAGGGAATTATTATGTTAAATTAATGAAACATGCCggag                                                                                                                                             >  2:1622754/1‑148 (MQ=255)
        gATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCCAATAAACATCAGATGATGAGTGATGGTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcc                                                                                                                                           >  2:1685877/1‑150 (MQ=255)
        gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcc                                                                                                                                           <  2:1284716/150‑1 (MQ=255)
        gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcc                                                                                                                                           <  1:1897762/150‑1 (MQ=255)
        gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcc                                                                                                                                           <  1:1679191/150‑1 (MQ=255)
        gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGAAGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCAAAGGGGAATTATGATGTTTAATGCATGGCAACACCCGGAGcc                                                                                                                                           >  2:1337614/1‑150 (MQ=255)
         aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccc                                                                                                                                          <  1:1946133/150‑1 (MQ=255)
         aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGcca                                                                                                                                          <  1:2172139/150‑2 (MQ=255)
           ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatg                                                                                                                                        <  2:1901186/150‑4 (MQ=255)
           ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatg                                                                                                                                        <  2:1489997/150‑4 (MQ=255)
           ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatg                                                                                                                                        <  1:1708325/150‑4 (MQ=255)
            cGGCTATTCCGAAAGTTATCGGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatgc                                                                                                                                       <  1:941635/150‑5 (MQ=255)
                tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                                                                                                                                   <  1:725551/150‑1 (MQ=255)
                     cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                                                                                                                              <  1:1489901/150‑1 (MQ=255)
                      gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGCTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                             <  2:2016418/150‑1 (MQ=255)
                          gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                                                                                                                         <  1:1622754/150‑1 (MQ=255)
                           ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                                                                                                                        <  2:2325423/150‑1 (MQ=255)
                                cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                                                                                                                   >  2:1762260/1‑150 (MQ=255)
                                cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                                                                                                                   <  1:1338428/150‑1 (MQ=255)
                                 gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt                                                                                                                  <  1:774555/150‑1 (MQ=255)
                                 gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt                                                                                                                  <  1:938388/150‑1 (MQ=255)
                                  cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCCAAGATc                                                                                                                 <  2:1619462/150‑1 (MQ=255)
                                  cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc                                                                                                                 >  1:2080164/1‑150 (MQ=255)
                                  cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc                                                                                                                 <  2:236380/150‑1 (MQ=255)
                                     ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc                                                                                                              <  2:1816353/150‑1 (MQ=255)
                                      cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt                                                                                                             <  1:1660039/150‑1 (MQ=255)
                                       gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg                                                                                                            >  2:2044326/1‑150 (MQ=255)
                                          ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGCGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc                                                                                                         <  1:101395/150‑1 (MQ=255)
                                           tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa                                                                                                        >  2:1959/1‑150 (MQ=255)
                                           tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa                                                                                                        <  2:1824843/150‑1 (MQ=255)
                                            cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt                                                                                                       <  2:2447895/150‑1 (MQ=255)
                                             ctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAtt                                                                                                      <  2:2440592/150‑1 (MQ=255)
                                              tccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTa                                                                                                     <  2:468151/150‑1 (MQ=255)
                                                 cccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                  <  2:1564467/150‑1 (MQ=255)
                                                  ccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACt                                                                                                 >  1:889904/1‑150 (MQ=255)
                                                       tatCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGc                                                                                            <  1:2183332/150‑1 (MQ=255)
                                                          ccTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGa                                                                                         >  2:1200228/1‑150 (MQ=255)
                                                           cTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGAc                                                                                        >  2:85319/1‑150 (MQ=255)
                                                               aGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGc                                                                                    >  2:1278804/1‑150 (MQ=255)
                                                                    atgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGa                                                                               <  2:307335/150‑1 (MQ=255)
                                                                     tgaGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGAc                                                                              <  1:667718/150‑1 (MQ=255)
                                                                     tgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGAc                                                                              <  1:331898/150‑1 (MQ=255)
                                                                       aGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGAccc                                                                            >  2:1747153/1‑150 (MQ=255)
                                                                       aGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGAccc                                                                            >  1:648612/1‑150 (MQ=255)
                                                                            tCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGc                                                                       <  1:71793/150‑1 (MQ=255)
                                                                              cTGCATTAGGCTATGGCATGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAg                                                                     >  2:2374352/1‑150 (MQ=255)
                                                                              cTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATCACACTGTGGCTGACGGGCGAAGACCCACCGCAg                                                                     <  2:1922813/150‑1 (MQ=255)
                                                                               tGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAgc                                                                    >  2:596150/1‑150 (MQ=255)
                                                                                gCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAgcg                                                                   <  2:1460802/150‑1 (MQ=255)
                                                                                   ttAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCATAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCAt                                                                <  2:1103475/150‑1 (MQ=255)
                                                                                   ttAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCAt                                                                <  2:651416/150‑1 (MQ=255)
                                                                                     aGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTa                                                              <  2:514433/150‑1 (MQ=255)
                                                                                        cTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAtgc                                                           >  1:2143201/1‑150 (MQ=255)
                                                                                         tATGGCAAGGTGATCAGATTTCCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAtgct                                                          >  2:1421548/1‑150 (MQ=255)
                                                                                           tGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAtgctgc                                                        >  1:1072705/1‑150 (MQ=255)
                                                                                              cAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTa                                                     <  1:518838/150‑1 (MQ=255)
                                                                                               aaGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTAc                                                    <  2:110603/150‑1 (MQ=255)
                                                                                               aaGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTAc                                                    <  1:270123/150‑1 (MQ=255)
                                                                                                aGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACa                                                   <  2:139190/150‑1 (MQ=255)
                                                                                                 ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTTCACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                  >  2:3749/1‑150 (MQ=255)
                                                                                                 ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                  >  1:1382874/1‑150 (MQ=255)
                                                                                                  gTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGa                                                 <  1:306229/150‑1 (MQ=255)
                                                                                                       cAGATTTTCATCACAGGGGATTTTTGATGTTAAATGCATGGCACCTGCCGGTGCCCCGATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACg                                            <  1:2320408/150‑1 (MQ=255)
                                                                                                       cAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACg                                            >  1:673181/1‑150 (MQ=255)
                                                                                                            tttCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAAc                                       <  1:1200228/150‑1 (MQ=255)
                                                                                                                 tcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACgaaga                                  >  2:2354538/1‑150 (MQ=255)
                                                                                                                 tcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACgaaga                                  >  2:2005643/1‑150 (MQ=255)
                                                                                                                     aGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATg                              <  1:2001703/150‑1 (MQ=255)
                                                                                                                       gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGTGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTc                            <  1:1337614/150‑1 (MQ=255)
                                                                                                                       gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTc                            <  2:190778/150‑1 (MQ=255)
                                                                                                                         gAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGACCACGATAACGAAGAAATGTCAg                          <  1:2205122/150‑1 (MQ=255)
                                                                                                                              atgaggTTAAATGCATGGCACCTGCCGGGGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCg                     <  2:2307963/150‑1 (MQ=255)
                                                                                                                               tgatgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACa                                                   >  2:906103/1‑119 (MQ=255)
                                                                                                                                 atgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATg                  <  2:817108/150‑1 (MQ=255)
                                                                                                                                 atgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATg                  >  2:132727/1‑150 (MQ=255)
                                                                                                                                 atgTTAAATGCATGGCACCTCCCGGTGAACCCATTTATTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGATGACGGGCAAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAAAGAAGAAATGTCAGTACCGATg                  >  2:1049236/1‑150 (MQ=255)
                                                                                                                                   gTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCa                >  1:2465218/1‑150 (MQ=255)
                                                                                                                                   gTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCa                <  2:711619/150‑1 (MQ=255)
                                                                                                                                   gTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCAATATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCa                >  1:1054696/1‑150 (MQ=255)
                                                                                                                                    ttAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCAt               <  1:701512/150‑1 (MQ=255)
                                                                                                                                     tAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATa              >  1:866655/1‑150 (MQ=255)
                                                                                                                                      aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCa                           >  1:1897592/1‑136 (MQ=255)
                                                                                                                                      aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCa                           <  2:1897592/136‑1 (MQ=255)
                                                                                                                                       aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAag            >  1:2127237/1‑150 (MQ=255)
                                                                                                                                        aTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGTTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagc           <  1:2374352/150‑1 (MQ=255)
                                                                                                                                        aTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagc           <  1:513823/150‑1 (MQ=255)
                                                                                                                                         tGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagca          >  1:1442936/1‑150 (MQ=255)
                                                                                                                                         atgggggCACCGGCAGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagca          >  2:1972375/6‑150 (MQ=255)
                                                                                                                                            ggggCACCGGCAGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg       >  2:190351/3‑150 (MQ=255)
                                                                                                                                            ggggCACCGGCAGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg       >  1:2227628/3‑150 (MQ=255)
                                                                                                                                             tGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAGCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcgc      <  1:744041/150‑1 (MQ=255)
                                                                                                                                              ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCa     >  1:1237998/1‑150 (MQ=255)
                                                                                                                                                 aCCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTc  >  2:1742592/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTC  >  NC_000913/422389‑422683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑