Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,315,437 A→T 5.9% F34L (TTT→TTA) ‡ phnO ← aminoalkylphosphonate N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,315,4370AT 5.9% 581.8 / 14.5 182F34L (TTT→TTA) ‡phnOaminoalkylphosphonate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (83/88);  new base T (7/4);  total (90/92)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

ACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACT  >  NC_000913/4315288‑4315586
                                                                                                                                                     |                                                                                                                                                     
 ccaccaTCTCCTGAATTTCGGCGTTCCAGTTGTCATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCGGACAACTTCGGCATCTAGCTGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaa                                                                                                                                                      <  2:1641553/150‑1 (MQ=255)
 ccaccaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaa                                                                                                                                                      >  1:1413499/1‑150 (MQ=255)
          ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtgg                                                                                                                                              <  2:646725/149‑1 (MQ=255)
          ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtgg                                                                                                                                              >  1:646725/1‑149 (MQ=255)
           cTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtgggt                                                                                                                                            <  2:1218782/150‑3 (MQ=255)
           cTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtggtg                                                                                                                                            <  1:320027/150‑1 (MQ=255)
             gAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtgggttt                                                                                                                                          <  2:1026635/150‑5 (MQ=255)
                  tCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAAc                                                                                                                                     >  1:2345178/1‑150 (MQ=255)
                    gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTc                                                                                                                                   >  1:1756804/1‑150 (MQ=255)
                     ccGATCCATTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAATCAGCGCCAGATGGTAGCGCATGTTTGTGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGAGAGGTGGTCAAACTcc                                                                                                                                  >  2:2449709/1‑150 (MQ=255)
                       gATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGc                                                                                                                                >  2:2347333/1‑150 (MQ=255)
                        aTCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGcc                                                                                                                               <  1:2230849/150‑1 (MQ=255)
                           cAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTTGCGTTAAAACCCACGCGAAAAGCGTGGTGGTAAAACTCAGCCTGt                                                                                                                            >  2:1040557/1‑150 (MQ=255)
                           cAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGt                                                                                                                            >  2:1292313/1‑150 (MQ=255)
                           cAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGt                                                                                                                            >  2:123890/1‑150 (MQ=255)
                                 aCATTATGCAGATGAAACTTCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGTTAGAGCATGTTTGTGTCGCGCATATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGc                                                                                                                      <  2:1085430/150‑1 (MQ=255)
                                     gatgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTcac                                                                                                                  <  1:1807148/150‑1 (MQ=255)
                                     gatgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTcac                                                                                                                  <  2:192480/150‑1 (MQ=255)
                                       tgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTC‑AACTCCGCCTGTTTTAGCTCACaaa                                                                                                               >  2:2478758/1‑150 (MQ=255)
                                       tgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCACATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACaa                                                                                                                >  1:242836/1‑150 (MQ=255)
                                           gATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCa                                                                                                            <  1:1505727/150‑1 (MQ=255)
                                             tGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgc                                                                                                          >  1:1617650/1‑150 (MQ=255)
                                              gAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCTCGCAGATTGGCGTTAAAACCCACGGGAAACGCGTGGTGGTCAAACTCGTCGTGTTTTTTCTCAGATATCAgcg                                                                                                         <  2:351813/150‑1 (MQ=255)
                                              gAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgcg                                                                                                         >  1:1922014/1‑150 (MQ=255)
                                                aaCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGcgcg                                                                                                       >  2:1965714/1‑150 (MQ=255)
                                                 aCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGt                                                                                                      <  2:1146826/150‑1 (MQ=255)
                                                 aCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGt                                                                                                      <  1:1016630/150‑1 (MQ=255)
                                                  cTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTa                                                                                                     >  1:366005/1‑150 (MQ=255)
                                                     cAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAc                                                                                                  >  2:793688/1‑150 (MQ=255)
                                                       aaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACcg                                                                                                >  1:481263/1‑150 (MQ=255)
                                                           cAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTc                                                                                            <  1:1004647/150‑1 (MQ=255)
                                                            aGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCg                                                                                           >  1:1826594/1‑150 (MQ=255)
                                                             ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCgg                                                                                          <  1:1600291/150‑1 (MQ=255)
                                                             ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCgg                                                                                          <  1:1427033/150‑1 (MQ=255)
                                                             ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCACCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCgg                                                                                          >  1:1441871/1‑150 (MQ=255)
                                                               ccGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGgtg                                                                                        >  2:1618410/1‑150 (MQ=255)
                                                                cGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGgtgt                                                                                       >  1:1276680/1‑150 (MQ=255)
                                                                 gATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTc                                                                                      >  2:1709881/1‑150 (MQ=255)
                                                                   tCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGt                                                                                    <  1:1841354/150‑1 (MQ=255)
                                                                      tGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACt                                                                                 <  2:2047819/150‑1 (MQ=255)
                                                                       gCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTg                                                                                >  2:306784/1‑150 (MQ=255)
                                                                        ccGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGc                                                                               >  2:1812381/1‑150 (MQ=255)
                                                                            cAACTTCGCCATCAAGCAACGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGATTTAGCTCACAAATCAGAGCGTAAACCGCGTCGCTCTCGTACTGCGTgg                                                                           >  2:2291751/1‑150 (MQ=255)
                                                                              aCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAACCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                                                                         >  1:647570/1‑150 (MQ=255)
                                                                               cTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCg                                                                        >  1:1130895/1‑150 (MQ=255)
                                                                                ttCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCgg                                                                       <  2:1756804/150‑1 (MQ=255)
                                                                                ttCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCgg                                                                       <  1:788683/150‑1 (MQ=255)
                                                                                 tCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCggg                                                                      <  1:2093674/150‑1 (MQ=255)
                                                                                 tCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCggg                                                                      <  1:306784/150‑1 (MQ=255)
                                                                                  cGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGc                                                                     <  2:1360547/150‑1 (MQ=255)
                                                                                   gCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCg                                                                    <  1:1084600/150‑1 (MQ=255)
                                                                                      aTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAg                                                                 >  2:274852/1‑150 (MQ=255)
                                                                                        cAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                               <  1:2471287/150‑1 (MQ=255)
                                                                                        cAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                               >  1:1516665/1‑150 (MQ=255)
                                                                                         aaGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTc                                                              <  2:2216785/150‑1 (MQ=255)
                                                                                             agcGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACaa                                                          <  1:818087/150‑1 (MQ=255)
                                                                                                gcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCa                                                       >  2:2028399/1‑150 (MQ=255)
                                                                                                gcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGAGCGTAAACCGCGTCGCTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCa                                                       >  1:2278799/1‑150 (MQ=255)
                                                                                                  cAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAgg                                                     <  1:2171945/150‑1 (MQ=255)
                                                                                                      tGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATg                                                 <  1:845381/150‑1 (MQ=255)
                                                                                                       ggTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATgg                                                <  1:957615/150‑1 (MQ=255)
                                                                                                         tAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtg                                              <  2:694315/150‑1 (MQ=255)
                                                                                                             gcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtc                                          <  2:1276680/150‑1 (MQ=255)
                                                                                                             gcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGCACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtc                                          <  1:1705181/150‑1 (MQ=255)
                                                                                                                tGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTAGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTcc                                       <  2:1613344/150‑1 (MQ=255)
                                                                                                                tGTTTGGGGCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTcc                                       <  1:1676335/150‑1 (MQ=255)
                                                                                                                 gTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCt                                      <  2:1062539/150‑1 (MQ=255)
                                                                                                                   ttGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTc                                    <  2:84508/150‑1 (MQ=255)
                                                                                                                   ttGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTc                                    <  1:1153504/150‑1 (MQ=255)
                                                                                                                    tGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCt                                   <  2:1917450/150‑1 (MQ=255)
                                                                                                                    tGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCt                                   <  2:951318/150‑1 (MQ=255)
                                                                                                                     gggTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTg                                  <  1:1190878/150‑1 (MQ=255)
                                                                                                                      ggTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGa                                 >  2:624984/1‑150 (MQ=255)
                                                                                                                      ggTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGa                                 <  2:1337774/150‑1 (MQ=255)
                                                                                                                           cgcAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGAt                            <  1:274852/150‑1 (MQ=255)
                                                                                                                             cAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCa                          <  2:1901261/150‑1 (MQ=255)
                                                                                                                              aGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAg                         <  2:49057/150‑1 (MQ=255)
                                                                                                                                aTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCg                       >  2:1785137/1‑150 (MQ=255)
                                                                                                                                aTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCg                       <  2:722465/150‑1 (MQ=255)
                                                                                                                                 ttGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGt                      >  1:1226801/1‑150 (MQ=255)
                                                                                                                                   ggCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTca                    >  1:672898/1‑150 (MQ=255)
                                                                                                                                   ggCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTca                    <  2:1413499/150‑1 (MQ=255)
                                                                                                                                   ggCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGAGTTTCCGGGCGAAGCTCACAAGCAGGCATGGTGGTTCTGCTTCTGATGGATCAGCGTca                    <  2:2009927/150‑1 (MQ=255)
                                                                                                                                     cGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagc                  <  1:2235109/150‑1 (MQ=255)
                                                                                                                                      gTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagca                 >  1:546359/1‑150 (MQ=255)
                                                                                                                                        tAAAACCCACGCGAAACGCGTGGTGGTCAAAGTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCagcagc               >  2:1486217/1‑150 (MQ=255)
                                                                                                                                        tAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCagcagc               >  1:572144/1‑150 (MQ=255)
                                                                                                                                           ccaccACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgtg            >  2:41867/4‑150 (MQ=255)
                                                                                                                                            caccACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgtgt           >  2:2171549/3‑150 (MQ=255)
                                                                                                                                            aCCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCa                          >  2:2062425/1‑135 (MQ=255)
                                                                                                                                            aCCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCa                          <  1:2062425/135‑1 (MQ=255)
                                                                                                                                             cccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTc          >  2:2055164/1‑150 (MQ=255)
                                                                                                                                             accACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTc          >  2:614304/2‑150 (MQ=255)
                                                                                                                                             accACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTc          >  2:1096653/2‑150 (MQ=255)
                                                                                                                                              ccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcg         >  1:1046130/1‑150 (MQ=255)
                                                                                                                                                aCGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgac       <  1:71434/150‑1 (MQ=255)
                                                                                                                                                 cgcgAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgacc      <  2:366005/150‑1 (MQ=255)
                                                                                                                                                 cgcgAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgacc      <  1:296831/150‑1 (MQ=255)
                                                                                                                                                 cgcgAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgacc      <  1:2020837/150‑1 (MQ=255)
                                                                                                                                                  gcgAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgaccg     <  1:1709881/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACT  >  NC_000913/4315288‑4315586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑