BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,400,330	C→G	6.0%	L15V (CTG→GTG)	hfq →	RNA‑binding protein Hfq

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,330	0	C	G	6.0%	664.1 / 19.8	200	L15V (CTG→GTG)	hfq	RNA‑binding protein Hfq
Reads supporting (aligned to +/- strand): ref base C (94/90); new base G (9/4); total (106/94)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

TGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCG  >  NC_000913/4400181‑4400479

 gttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACt                                                                                                                                                       <  1:1805953/150‑1 (MQ=255)

      tGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑Tc                                                                                                                                                 >  1:1454702/1‑150 (MQ=255)

       gCTATCGCGGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCg                                                                                                                                                >  2:853977/1‑150 (MQ=255)

        cTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCgg                                                                                                                                               <  2:946470/150‑1 (MQ=255)

          aTCGCAGGCTGAATGTGTACATTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGGTCAAAGTACAAAGAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGa                                                                                                                                             >  2:1824518/1‑150 (MQ=255)

            cGCAGGCTGAATGTGAACAAATGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAAc                                                                                                                                           >  2:9215/1‑150 (MQ=255)

             gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                          >  2:195556/1‑150 (MQ=255)

             gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                          <  2:1564076/150‑1 (MQ=255)

             gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCCG‑ACGCAGTGCGTTCAGGAACg                                                                                                                                          <  2:1437037/150‑1 (MQ=255)

                ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                                       <  2:44605/150‑1 (MQ=255)

                ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCCG‑ACGCAGTGCGTTCAGGAACggat                                                                                                                                       <  2:1426208/150‑4 (MQ=255)

                 gCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGtt                                                                                                                                      <  1:416242/150‑1 (MQ=255)

                  cTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTc                                                                                                                                     >  1:319108/1‑150 (MQ=255)

                     aaTGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTACTGAACGCACTGCG‑TCGGGAACGTGTTCCAg                                                                                                                                  >  1:1256013/1‑150 (MQ=255)

                         tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                              <  2:2169925/150‑1 (MQ=255)

                          gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCt                                                                                                                             >  2:2359527/1‑150 (MQ=255)

                          gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCt                                                                                                                             >  2:2336213/1‑150 (MQ=255)

                          gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCt                                                                                                                             >  1:412820/1‑150 (MQ=255)

                           tACTATTGAGACGTTTCTTGCGCAATTTTTTAAGAATGGATATGTTAAAAGTACAAATTAGAATATATGGTAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCAGTGCG‑TCGGTAACGGGTTCCAGTTTCta                                                                                                                            <  2:1120551/150‑1 (MQ=255)

                           tACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAACGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCta                                                                                                                            >  2:1918931/1‑150 (MQ=255)

                             cAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtatt                                                                                                                          >  1:1193845/1‑150 (MQ=255)

                             cAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTACAGTTTCtatt                                                                                                                          >  1:1287186/1‑150 (MQ=255)

                              aaTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtattt                                                                                                                         <  2:1268509/150‑1 (MQ=255)

                                ttGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGCATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtatttat                                                                                                                       >  2:795343/1‑150 (MQ=255)

                                ttGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtatttat                                                                                                                       >  1:1986258/1‑150 (MQ=255)

                                  gagaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTatttattt                                                                                                                     <  1:571914/150‑1 (MQ=255)

                                  gagaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTatttattt                                                                                                                     >  1:1535008/1‑150 (MQ=255)

                                   agaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTg                                                                                                                    <  2:1368239/150‑1 (MQ=255)

                                    gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTgg                                                                                                                   >  2:2453748/1‑150 (MQ=255)

                                    gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTgg                                                                                                                   >  2:2022300/1‑150 (MQ=255)

                                      cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                                                                                                 <  1:727647/150‑1 (MQ=255)

                                         aTCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAACTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAAt                                                                                                              <  1:673606/150‑1 (MQ=255)

                                          tCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                                                                                             <  2:463904/150‑1 (MQ=255)

                                            gTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATATTTACAAGATCCGTTCCTGAAAGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGt                                                                                                           >  1:345299/1‑150 (MQ=255)

                                              gcgcAATTTTATCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                                                                                         <  2:1768392/150‑1 (MQ=255)

                                                gcatattttaCAGAAACAAAATTTTAAAATTAAAAAAAAGCATATAAGCAAAAGAGAAAATAACTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                                                                                                       >  2:1335245/6‑150 (MQ=255)

                                                gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTg                                                                                                                    >  1:2230459/1‑137 (MQ=255)

                                                gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTg                                                                                                                    <  2:2230459/137‑1 (MQ=255)

                                                 cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                                                                                      >  2:2070902/1‑150 (MQ=255)

                                                   aTTTTTTCAGAATCGATAGGTTCAAAGTACAAATAATCATATAAGGAAAATAGATAATGGCTATGGGGCTATCTTTACAAGTTGCGTTCCTGAACGCACTGCT‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                                                                                                    <  1:1033947/150‑1 (MQ=255)

                                                     tttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTg                                                                                                  >  1:786798/1‑150 (MQ=255)

                                                      ttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                                                                                      >  2:1189401/1‑145 (MQ=255)

                                                          cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                                                                                             <  2:1042734/150‑1 (MQ=255)

                                                           aGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAggg                                                                                            <  1:2449230/150‑1 (MQ=255)

                                                              aTCGAAAGGTTAAAAGTACAAATAAGTATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATATTTACAAGATCCGTTCATGAACGCACTGAG‑TCGGGAACGTTTTCAAGATTCTAATTATTTGCTGAAAGGTATTTAGGTGCAAtggaga                                                                                         >  2:1079048/1‑147 (MQ=255)

                                                                cGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAAt                                                                                       >  2:274108/1‑150 (MQ=255)

                                                                 gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                                                                                      >  1:1390062/1‑150 (MQ=255)

                                                                 gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTATACAACATCAGTTCCTGAACGCAATGCG‑TCAGGAACCTGTTCCAGTTTCTATTTATTTGGTGAATGGTTTTAAGCTGCAAGGGCAAATc                                                                                      >  1:814341/1‑150 (MQ=255)

                                                                    aGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg                                                                                   <  1:2022300/150‑1 (MQ=255)

                                                                       ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCt                                                                                >  1:441419/1‑150 (MQ=255)

                                                                        tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtt                                                                               >  2:1291804/1‑150 (MQ=255)

                                                                        tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGCAACGAGTTCCAGTTACTATTTATTTGGTCAATGGTATTAAGCTCCAAGGGCAAATCGAGTCta                                                                               >  1:941848/1‑149 (MQ=255)

                                                                          aaaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt                                                                             <  1:603371/150‑1 (MQ=255)

                                                                           aaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg                                                                            <  1:260397/150‑1 (MQ=255)

                                                                             gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGAt                                                                          >  2:324673/1‑150 (MQ=255)

                                                                               aCAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCa                                                                        <  1:225670/150‑1 (MQ=255)

                                                                                cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATACGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGATGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAg                                                                       >  2:1515528/1‑150 (MQ=255)

                                                                                    tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCg                                                                   >  1:1111508/1‑150 (MQ=255)

                                                                                    tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCg                                                                   <  2:412820/150‑1 (MQ=255)

                                                                                    tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCg                                                                   >  1:454104/1‑150 (MQ=255)

                                                                                          tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                             >  1:2291091/1‑150 (MQ=255)

                                                                                          tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                             <  1:1783772/150‑1 (MQ=255)

                                                                                          tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                             <  1:364883/150‑1 (MQ=255)

                                                                                             aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGt                                                          >  1:1123471/1‑150 (MQ=255)

                                                                                              aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                             >  1:1686495/1‑146 (MQ=255)

                                                                                              aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                             <  2:1686495/146‑1 (MQ=255)

                                                                                              aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGCAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGATGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCATGtt                                                         >  1:2186989/1‑150 (MQ=255)

                                                                                               ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                        >  1:1676163/1‑150 (MQ=255)

                                                                                                gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGa                                                       >  1:1945099/1‑150 (MQ=255)

                                                                                                gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGa                                                       <  2:712988/150‑1 (MQ=255)

                                                                                                     gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                  <  2:971941/150‑1 (MQ=255)

                                                                                                     gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                  <  2:1397331/150‑1 (MQ=255)

                                                                                                     gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                  <  1:1731109/150‑1 (MQ=255)

                                                                                                      agagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAaca                                                 <  1:2158957/150‑1 (MQ=255)

                                                                                                       gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAacac                                                <  2:365201/150‑1 (MQ=255)

                                                                                                       gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAacac                                                <  1:1291804/150‑1 (MQ=255)

                                                                                                         gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACgg                                              <  2:916458/150‑1 (MQ=255)

                                                                                                             ggCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAg                                          >  2:554012/1‑150 (MQ=255)

                                                                                                                      gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGt                                 >  1:1748151/1‑150 (MQ=255)

                                                                                                                       cAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                >  1:1640481/1‑150 (MQ=255)

                                                                                                                        aaTCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGttt                               <  1:980948/150‑1 (MQ=255)

                                                                                                                         aTCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTa                              <  1:1136176/150‑1 (MQ=255)

                                                                                                                          tCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                             >  1:1188320/1‑150 (MQ=255)

                                                                                                                           cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                            <  1:2417918/150‑1 (MQ=255)

                                                                                                                           cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                            <  1:9215/150‑1 (MQ=255)

                                                                                                                           cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                            <  2:2138982/150‑1 (MQ=255)

                                                                                                                               aCAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCa                        >  2:2398907/1‑150 (MQ=255)

                                                                                                                                cAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                       >  1:1508199/1‑150 (MQ=255)

                                                                                                                                cAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAAAGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTTATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAAAAGCaa                       >  2:842182/1‑149 (MQ=255)

                                                                                                                                 ggaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                      >  2:1001921/7‑150 (MQ=255)

                                                                                                                                 ggaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                      >  1:2063791/7‑150 (MQ=255)

                                                                                                                                  gaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgc                     >  2:1217689/6‑150 (MQ=255)

                                                                                                                                  gaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATACTGTTGAAAAACACGGTCAGCCAGAGGGTTTACAAGCCcgc                     >  2:2113289/6‑150 (MQ=255)

                                                                                                                                   aacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg                    >  2:1659319/5‑150 (MQ=255)

                                                                                                                                     tCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAt                  >  2:262769/1‑150 (MQ=255)

                                                                                                                                           ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc            >  2:657814/1‑150 (MQ=255)

                                                                                                                                           ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc            <  1:850724/150‑1 (MQ=255)

                                                                                                                                            cTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt           <  2:2291091/150‑1 (MQ=255)

                                                                                                                                            cTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt           >  1:37067/1‑150 (MQ=255)

                                                                                                                                             tGAAGGCAGTGCG‑TCGGGAACGTGTTCCAGTTTCTTTTTATTTGGTGAATGGTATTAAGCTGGAAGGGCAAATGGAGTCTTTTGATGTGTTGGTGATCCTGTTGAAAAAGACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtg          <  2:941848/150‑1 (MQ=255)

TGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCG  >  NC_000913/4400181‑4400479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`