Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,129 C→T 5.3% I65I (ATC→ATT) ‡ fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1290CT 5.3% 490.7 / 9.7 151I65I (ATC→ATT) ‡fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB
Reads supporting (aligned to +/- strand):  ref base C (70/73);  new base T (4/4);  total (74/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 153 total aligned reads displayed.

GCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGG  >  NC_000913/4428981‑4429275
                                                                                                                                                     |                                                                                                                                                   
gCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATcc                                                                                                                                                    <  1:2318401/150‑1 (MQ=255)
gCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATcc                                                                                                                                                    >  1:164211/1‑150 (MQ=255)
      gTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agc                                                                                                                                             >  1:2232455/1‑150 (MQ=255)
      gTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGAATC‑GCGCGCTGCGTGAAATCAACG‑Agc                                                                                                                                             >  1:819186/1‑150 (MQ=255)
         tGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcg                                                                                                                                          <  1:695784/150‑1 (MQ=255)
         tGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTGGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcg                                                                                                                                          <  1:1089828/150‑1 (MQ=255)
          gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAgcgcg                                                                                                                                          <  1:930936/150‑1 (MQ=255)
          gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcgc                                                                                                                                         >  2:252604/1‑150 (MQ=255)
          gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcgc                                                                                                                                         >  2:2111862/1‑150 (MQ=255)
           cAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGcc                                                                                                                                        >  1:1597744/1‑150 (MQ=255)
           cAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTACGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGcc                                                                                                                                        >  2:1023077/1‑150 (MQ=255)
             ggTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGa                                                                                                                                      >  1:295322/1‑150 (MQ=255)
              gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGAt                                                                                                                                     >  1:691191/1‑150 (MQ=255)
              gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGAt                                                                                                                                     <  1:2251737/150‑1 (MQ=255)
               tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAgcgcgcgatg                                                                                                                                     <  1:1312598/150‑5 (MQ=38)
               tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATg                                                                                                                                    >  2:2454044/1‑150 (MQ=255)
               tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATg                                                                                                                                    <  2:228017/150‑1 (MQ=255)
                 gggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGcc                                                                                                                                  >  1:1693970/1‑150 (MQ=255)
                    caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                               >  2:769506/1‑150 (MQ=255)
                       caacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTtcgt                                                                                                                            >  1:667386/1‑150 (MQ=255)
                         acTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTtcgtcg                                                                                                                          <  2:2114891/150‑1 (MQ=255)
                           tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCgtcgtc                                                                                                                        >  2:2396238/1‑150 (MQ=255)
                            gAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCa                                                                                                                       <  1:2210470/150‑1 (MQ=255)
                              gTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGTAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGc                                                                                                                     <  2:367433/150‑1 (MQ=255)
                              gTGAATCTGGCCTGGAAGGGCTGATGCCAGAAGCACTGGTTGCAGGTATTGCCGATCCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑ACCGCGCCGATGCCGTTCGTCGTCAGc                                                                                                                     >  1:1230178/1‑150 (MQ=255)
                               tGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCg                                                                                                                    <  1:2118317/150‑1 (MQ=255)
                                  aTCTGGCCTGGAAGGGCTGCTGCCAGAAGAACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGttt                                                                                                                 >  1:253024/1‑150 (MQ=255)
                                      ggCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCa                                                                                                              >  2:1588730/1‑149 (MQ=255)
                                      ggCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCa                                                                                                              <  1:1588730/149‑1 (MQ=255)
                                      ggCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAg                                                                                                             >  1:455147/1‑150 (MQ=255)
                                       gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            <  1:2346527/150‑1 (MQ=255)
                                       gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            >  2:1950550/1‑150 (MQ=255)
                                        ccTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGc                                                                                                           >  2:1067245/1‑150 (MQ=255)
                                         cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                          >  2:1504059/1‑150 (MQ=255)
                                          tGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGa                                                                                                         >  1:1024604/1‑150 (MQ=255)
                                              aGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                                     >  2:197099/1‑150 (MQ=255)
                                              aGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                                     >  1:1580424/1‑150 (MQ=255)
                                               gggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgct                                                                                                    >  1:1979102/1‑150 (MQ=255)
                                                ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                                   >  2:1847885/1‑150 (MQ=255)
                                                   tgctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGctgctg                                                                                                <  1:492295/150‑1 (MQ=255)
                                                      tgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAg                                                                                             >  2:139077/1‑150 (MQ=255)
                                                      tgcCAGAAGCACTGATTGAACGTATTTCAGATGCGCTGGAAGGCAAACATCCGGCTCTACATATTAATGTGCTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCCCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGaaa                                                                                             >  2:308761/1‑149 (MQ=255)
                                                         cAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                          >  1:2067638/1‑150 (MQ=255)
                                                         cAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                          >  2:498675/1‑150 (MQ=255)
                                                            aaGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGa                                                                                       <  1:832141/150‑1 (MQ=255)
                                                               cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                    >  1:1861600/1‑150 (MQ=255)
                                                                aCTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATa                                                                                   <  1:2396238/150‑1 (MQ=255)
                                                                  tGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATAcc                                                                                 <  2:1205031/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTTAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                               <  2:283864/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                               <  2:253024/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                               >  2:2158625/1‑150 (MQ=255)
                                                                        cAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                           <  1:1730343/150‑1 (MQ=255)
                                                                          ggTATTGCCGATGCGCTGGGAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaagaa                                                                         <  1:962526/150‑1 (MQ=255)
                                                                            tATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGaaaa                                                                       <  1:1090916/150‑1 (MQ=255)
                                                                              ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACg                                                                     <  1:994129/150‑1 (MQ=255)
                                                                                 ccGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑GGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCa                                                                  >  1:397651/1‑150 (MQ=255)
                                                                                 ccGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCa                                                                  >  1:89071/1‑150 (MQ=255)
                                                                                  cGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaa                                                                 <  2:1024604/150‑1 (MQ=255)
                                                                                   gATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaa                                                                <  2:1441525/150‑1 (MQ=255)
                                                                                   gATGCGCGGGAAGGCAAACATCCGGCGGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaa                                                                <  1:1792071/150‑1 (MQ=255)
                                                                                       cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                            >  2:293962/1‑150 (MQ=255)
                                                                                         cTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaa                                                          >  1:179183/1‑150 (MQ=255)
                                                                                         cTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaa                                                          >  1:1049116/1‑150 (MQ=255)
                                                                                          tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                         <  2:1734938/150‑1 (MQ=255)
                                                                                          tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                         <  1:1343804/150‑1 (MQ=255)
                                                                                          tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                         <  1:1156267/150‑1 (MQ=255)
                                                                                              aGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGgtgt                                                     <  2:1345816/150‑1 (MQ=255)
                                                                                               tgCAAACATGCGGCTGTTGCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑ATCGCGCCGATGCCGTTCGTCGTCAGCGTTTCGAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                    <  2:869395/149‑1 (MQ=255)
                                                                                               ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                    >  2:689652/1‑150 (MQ=255)
                                                                                               ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                    <  2:2473388/150‑1 (MQ=255)
                                                                                                 cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                  >  1:107181/1‑150 (MQ=255)
                                                                                                 cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                  >  1:206171/1‑150 (MQ=255)
                                                                                                 cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                  >  1:2163268/1‑150 (MQ=255)
                                                                                                  aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGCCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAAt                                                 <  2:164211/150‑1 (MQ=255)
                                                                                                   aaCATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATa                                                <  1:2480633/150‑1 (MQ=255)
                                                                                                     cATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCATCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGc                                              <  1:2454044/150‑1 (MQ=255)
                                                                                                        ccGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCAcc                                           <  1:1922329/150‑1 (MQ=255)
                                                                                                         cGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCAccc                                          >  1:2424544/1‑149 (MQ=255)
                                                                                                               ttCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                    >  1:1575548/1‑150 (MQ=255)
                                                                                                                tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTgg                                   >  2:1899197/1‑150 (MQ=255)
                                                                                                                tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTgg                                   <  1:740911/150‑1 (MQ=255)
                                                                                                                 ccGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGc                                  <  1:2369849/150‑1 (MQ=255)
                                                                                                                  cGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                                 >  2:2139338/1‑150 (MQ=255)
                                                                                                                         tgtgGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAAtt                          >  2:503253/1‑150 (MQ=255)
                                                                                                                         tgtgGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAAtt                          >  1:1861991/1‑150 (MQ=255)
                                                                                                                            ggtgCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcg                       >  1:1266038/1‑150 (MQ=255)
                                                                                                                             cggCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAACCGCCAAAAAAGAAGGTGTGAATAGGCCCGAAGCTGGCCTGCAATTCcgc                      >  1:416718/3‑150 (MQ=25)
                                                                                                                               gCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                    >  1:2146936/1‑150 (MQ=38)
                                                                                                                               gCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                    >  2:1096475/1‑150 (MQ=255)
                                                                                                                                          cTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg          <  2:455147/150‑1 (MQ=255)
                                                                                                                                          cTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg          <  2:1414447/150‑1 (MQ=255)
                                                                                                                                           tGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAgg         <  2:1326252/150‑1 (MQ=255)
                                                                                                                                            gCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAggg        >  2:1172507/1‑150 (MQ=255)
                                                                                                                                            gCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAGCACCCAggg        >  2:1616423/1‑150 (MQ=255)
                                                                                                                                             ggTGAAATCCACG‑AGCGCGCCGATGCCGTAGGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGt       <  2:2277016/149‑1 (MQ=255)
                                                                                                                                                gAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGaa    >  1:1180348/1‑150 (MQ=255)
                                                                                                                                                gAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGaa    <  1:1067245/150‑1 (MQ=255)
                                                                                                                                                 aaaTCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAg   <  2:405240/150‑1 (MQ=255)
                                                                                                                                                 aaaTCCACG‑AGCGCGCCGATGCCGTGCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTTAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAg   <  2:1746301/150‑1 (MQ=255)
                                                                                                                                                  aaTCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg  <  1:599695/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGG  >  NC_000913/4428981‑4429275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑