Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,552,185 G→C 6.3% A184P (GCG→CCG)  uxuA → D‑mannonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,552,1850GC 6.3% 591.2 / 21.9 176A184P (GCG→CCG) uxuAD‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (76/88);  new base C (3/8);  total (79/97)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 179 total aligned reads displayed.

ATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGCTGTTCA  >  NC_000913/4552040‑4552333
                                                                                                                                                 |                                                                                                                                                    
aTTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGga                                                                                                                                                  <  2:422507/150‑1 (MQ=255)
  tCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGgaag                                                                                                                                                >  2:571609/1‑150 (MQ=255)
   cGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGgaaga                                                                                                                                               <  1:1581488/150‑1 (MQ=255)
    gAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGGCAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGgaagaa                                                                                                                                              >  2:300477/1‑150 (MQ=255)
    gAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGgaagaa                                                                                                                                              >  2:2238874/1‑150 (MQ=255)
    gAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGgaagac                                                                                                                                              <  2:1002868/150‑2 (MQ=255)
     aaaTGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGgaagacc                                                                                                                                             <  1:2357235/150‑3 (MQ=255)
     aaaTGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCAATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGgaagacc                                                                                                                                             <  2:1015706/150‑3 (MQ=255)
      aaTGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAgg                                                                                                                                            >  1:32597/1‑150 (MQ=255)
       aTGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGc                                                                                                                                           <  2:2178958/150‑1 (MQ=255)
       aTGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGgaagaccag                                                                                                                                           <  1:272388/150‑5 (MQ=255)
        tGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCt                                                                                                                                          >  1:932675/1‑150 (MQ=255)
          cATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTac                                                                                                                                        >  2:2325116/1‑150 (MQ=255)
               ccTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTTTTCTTCCGGGATAGGAAGAAGGCTACActct                                                                                                                                   <  2:665842/150‑1 (MQ=255)
               ccTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTTGTCTTCCGGGCGCGGAAGAAGGCTACACCCt                                                                                                                                   <  1:166832/150‑1 (MQ=255)
               ccTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCt                                                                                                                                   >  1:2440634/1‑150 (MQ=255)
               ccTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCt                                                                                                                                   >  1:2066524/1‑150 (MQ=255)
               ccTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCt                                                                                                                                   >  1:460435/1‑150 (MQ=255)
                cTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTc                                                                                                                                  <  2:409214/150‑1 (MQ=255)
                      cGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAg                                                                                                                            <  2:2448032/150‑1 (MQ=255)
                        cccAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGtt                                                                                                                          >  2:2211255/1‑150 (MQ=255)
                           aGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCg                                                                                                                       <  2:715795/150‑1 (MQ=255)
                            ggCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGgaagacc                                                                                                                                             >  2:2282375/1‑125 (MQ=255)
                            ggCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGCGCCCGgaagacc                                                                                                                                             <  1:2282375/127‑3 (MQ=255)
                             gcgcGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTa                                                                                                                     <  1:1668274/150‑1 (MQ=255)
                             gcgcGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTa                                                                                                                     <  2:228710/150‑1 (MQ=255)
                              cgcgGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTaa                                                                                                                    <  1:2294453/150‑1 (MQ=255)
                                cgGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAac                                                                                                                  <  2:1737699/150‑1 (MQ=255)
                                cgGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAac                                                                                                                  <  2:642257/150‑1 (MQ=255)
                                cgGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAac                                                                                                                  >  1:1004611/1‑150 (MQ=255)
                                cgGAAGCGGATTACACCGAACAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAac                                                                                                                  >  2:597987/1‑150 (MQ=255)
                                 ggAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAaca                                                                                                                 <  1:2402729/150‑1 (MQ=255)
                                 ggAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAaca                                                                                                                 >  2:857941/1‑150 (MQ=255)
                                 ggAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAaca                                                                                                                 <  1:713180/150‑1 (MQ=255)
                                         aTTAGACCGAAGAAGAAATTGCTCAGGCAGCGGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGc                                                                                                         <  2:2266411/150‑1 (MQ=255)
                                         aTTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGC‑GAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCt                                                                                                        <  2:1019911/150‑1 (MQ=255)
                                             cacCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTa                                                                                                     <  1:1641877/150‑1 (MQ=255)
                                                cGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACaa                                                                                                  >  2:1418708/1‑150 (MQ=255)
                                                   agaagaaATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGa                                                                                               >  2:1199316/1‑150 (MQ=255)
                                                   agaagaaATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGa                                                                                               <  1:778992/150‑1 (MQ=255)
                                                    gaagaaATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGat                                                                                              >  2:1312791/1‑150 (MQ=255)
                                                      agaaATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGatat                                                                                            >  2:1294728/1‑150 (MQ=255)
                                                        aaaTTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCg                                                                                          <  1:1336058/150‑1 (MQ=255)
                                                         aaTTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGa                                                                                         <  1:2074069/150‑1 (MQ=255)
                                                                cAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCg                                                                                  >  2:952019/1‑150 (MQ=255)
                                                                cAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGAGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCg                                                                                  >  1:1916656/1‑150 (MQ=255)
                                                                    cAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGc                                                                              <  2:241072/150‑1 (MQ=255)
                                                                       cTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTgc                                                                           >  1:1058516/1‑150 (MQ=255)
                                                                             gCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGaaa                                                                     <  1:466858/150‑1 (MQ=255)
                                                                              cTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGaaaa                                                                    <  1:1979691/150‑1 (MQ=255)
                                                                              cTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGaaaa                                                                    >  1:2230428/1‑150 (MQ=255)
                                                                                 cGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACtt                                                                 >  1:270599/1‑150 (MQ=255)
                                                                                  gCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACttt                                                                >  1:140723/1‑150 (MQ=255)
                                                                                   ccACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTg                                                               >  2:2180723/1‑150 (MQ=255)
                                                                                    cACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGc                                                              <  1:856357/150‑1 (MQ=255)
                                                                                    cACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCATAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGc                                                              <  2:767622/150‑1 (MQ=255)
                                                                                     aCTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGcc                                                             >  1:251473/1‑150 (MQ=255)
                                                                                      cTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCg                                                            <  2:1533765/150‑1 (MQ=255)
                                                                                         tGAGCGATGAAGACAAAGCGCGTCTGACCCATAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCt                                                         >  2:334021/1‑150 (MQ=255)
                                                                                           aGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTc                                                       <  2:605589/150‑1 (MQ=255)
                                                                                            gCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTcc                                                      <  1:920915/150‑1 (MQ=255)
                                                                                            gCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTcc                                                      <  1:2238874/150‑1 (MQ=255)
                                                                                              gATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTg                                                    <  1:1460480/150‑1 (MQ=255)
                                                                                               aTGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCCGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGa                                                   >  2:2099504/1‑150 (MQ=255)
                                                                                                tGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGaa                                                  >  1:694408/1‑150 (MQ=255)
                                                                                                   aGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGc                                               <  2:1294953/150‑1 (MQ=255)
                                                                                                    gACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCg                                              >  2:81957/1‑150 (MQ=255)
                                                                                                      cAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGat                                            >  2:536691/1‑150 (MQ=255)
                                                                                                      cAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGat                                            <  1:68456/150‑1 (MQ=255)
                                                                                                       aaaGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGatt                                           <  1:2148717/150‑1 (MQ=255)
                                                                                                       aaaGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGatt                                           >  1:159256/1‑150 (MQ=255)
                                                                                                        aaGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGatta                                          <  2:804373/150‑1 (MQ=255)
                                                                                                        aaGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGatta                                          >  2:2228602/1‑150 (MQ=255)
                                                                                                           cgcgTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACACTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTc                                       >  1:593048/1‑150 (MQ=255)
                                                                                                            gcgTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTcc                                      <  1:639953/150‑1 (MQ=255)
                                                                                                             cgTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCa                                     >  1:1560447/1‑150 (MQ=255)
                                                                                                              gTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAg                                    <  1:1842478/150‑1 (MQ=255)
                                                                                                              gTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAg                                    <  2:1790168/150‑1 (MQ=255)
                                                                                                                cTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGtt                                  <  2:2066524/150‑1 (MQ=255)
                                                                                                                  gACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGc                                >  2:1769328/1‑150 (MQ=255)
                                                                                                                   aCCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCt                               >  2:1995568/1‑150 (MQ=255)
                                                                                                                        tAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTgaagt                          <  1:101833/150‑2 (MQ=255)
                                                                                                                           catcatTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGt                       >  2:474632/1‑150 (MQ=255)
                                                                                                                           catcatTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGt                       >  1:2352160/1‑150 (MQ=255)
                                                                                                                              catTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCgg                    <  1:1602746/150‑1 (MQ=255)
                                                                                                                              catTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATT‑CAGTTGCTGAAGAAGTCGgc                   <  1:1199316/150‑1 (MQ=255)
                                                                                                                                 tGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGgcgt                 >  1:38022/1‑150 (MQ=255)
                                                                                                                                   cTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGgcgtgc               <  1:1294728/150‑1 (MQ=255)
                                                                                                                                    tGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGgcgtgcg              <  1:2325116/150‑1 (MQ=255)
                                                                                                                                    ccttCTTCCGCGCCCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGgcgtgcg              >  1:978116/4‑150 (MQ=255)
                                                                                                                                     cttCTTCCGCGCCCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGgcgtgcgt             >  2:109177/3‑150 (MQ=255)
                                                                                                                                      gTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTa            <  1:439468/150‑1 (MQ=255)
                                                                                                                                        cTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATTTCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATg          >  2:1487361/1‑150 (MQ=255)
                                                                                                                                        cTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATg          >  1:912895/1‑150 (MQ=255)
                                                                                                                                          tCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGc        >  2:1004890/1‑150 (MQ=255)
                                                                                                                                          tCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGc        <  1:1261407/150‑1 (MQ=255)
                                                                                                                                           ccGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGCt       <  2:1688047/150‑1 (MQ=255)
                                                                                                                                              ggCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGCTGtt    <  1:2099504/150‑1 (MQ=255)
                                                                                                                                              ggCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGCTGtt    <  2:1557082/150‑1 (MQ=255)
                                                                                                                                               gcgcGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGCTGTTc   <  2:2344443/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACACCGAAGAAGAAATTGCTCAGGCAGCTGAACGCTTCGCCACTATGAGCGATGAAGACAAAGCGCGTCTGACCCGTAACATCATTGCTGGTCTTCCGGGCGCGGAAGAAGGCTACACCCTCGACCAGTTCCGTAAACACCTGGAGCTGTACAAAGATATCGACAAAGCGAAGCTGCGCGAAAACTTTGCCGTCTTCCTGAAAGCGATTATTCCAGTTGCTGAAGAAGTCGGCGTGCGTATGGCTGTTCA  >  NC_000913/4552040‑4552333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑