Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,558,215 G→C 5.1% C25W (TGC→TGG)  hypT ← DNA‑binding transcriptional dual regulator HypT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,558,2150GC 5.1% 543.9 / 13.6 158C25W (TGC→TGG) hypTDNA‑binding transcriptional dual regulator HypT
Reads supporting (aligned to +/- strand):  ref base G (73/77);  new base C (4/4);  total (77/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 160 total aligned reads displayed.

GGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTT  >  NC_000913/4558067‑4558364
                                                                                                                                                    |                                                                                                                                                     
 gAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCa                                                                                                                                                     <  2:1436095/150‑1 (MQ=255)
 gAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCa                                                                                                                                                     >  1:1633577/1‑150 (MQ=255)
 caaGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCa                                                                                                                                                     >  1:1890975/2‑150 (MQ=255)
  aaGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCAt                                                                                                                                                    >  1:2334145/1‑150 (MQ=255)
  aaGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCAt                                                                                                                                                    >  1:1819969/1‑150 (MQ=255)
        ttCCCTTGTTCTGAGAGGTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTGGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTcc                                                                                                                                              <  2:1547083/150‑1 (MQ=255)
         tCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCa                                                                                                                                             >  1:2480917/1‑150 (MQ=255)
          cccTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAGAAATTGCGGCATTTTTCCAg                                                                                                                                            <  1:203360/150‑1 (MQ=255)
          cccTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGAAAAAATTGCGGCATTTTTCCAg                                                                                                                                            <  1:606997/150‑1 (MQ=255)
           ccTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAgg                                                                                                                                           >  1:6651/1‑150 (MQ=255)
           ccTTGTTCTGAGAGTTGAAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGAAGCGGCGGCTGAATGACGGTTGCGAGACGATGCGACTGACTGCCCCCTGGGAAAAATTGCGGCAATTTTCCAgc                                                                                                                                           >  2:1415718/1‑149 (MQ=255)
            cTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCT‑GGAAAAATGCCGCAATTTTTcccaggc                                                                                                                                         <  2:607199/150‑6 (MQ=255)
            cTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTGAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATGCCGCAATTTTTcccagg                                                                                                                                          <  2:1306118/150‑5 (MQ=255)
              tGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTc                                                                                                                                        >  1:635937/1‑150 (MQ=255)
              tGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTc                                                                                                                                        <  2:1717718/150‑1 (MQ=255)
                ttCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAg                                                                                                                                      <  2:1190548/150‑1 (MQ=255)
                     agagTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAAt                                                                                                                                 >  2:643955/1‑150 (MQ=255)
                        gTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGGGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCAt                                                                                                                              >  2:336489/1‑150 (MQ=255)
                        gTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTTACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCAt                                                                                                                              <  2:1333619/150‑1 (MQ=255)
                        gTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCAt                                                                                                                              <  2:120907/150‑1 (MQ=255)
                         ttGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGTCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATa                                                                                                                             <  2:1091209/150‑1 (MQ=255)
                          tGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATaa                                                                                                                            <  1:988346/150‑1 (MQ=255)
                           gCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATaaa                                                                                                                           >  2:816669/1‑150 (MQ=255)
                            cAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAg                                                                                                                          <  2:2123397/150‑1 (MQ=255)
                                     tACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGt                                                                                                                 <  1:1103969/150‑1 (MQ=255)
                                       ccTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGttt                                                                                                               >  1:892629/1‑150 (MQ=255)
                                        cTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTc                                                                                                              <  1:2449393/150‑1 (MQ=255)
                                        cTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTc                                                                                                              <  1:2181382/150‑1 (MQ=255)
                                         tGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCa                                                                                                             >  1:1602129/1‑150 (MQ=255)
                                          ggCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCaa                                                                                                            <  1:1169696/150‑1 (MQ=255)
                                            cGGTTTAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAata                                                                                                          >  2:755003/1‑150 (MQ=255)
                                               ttAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAAtatta                                                                                                       >  2:1021619/1‑150 (MQ=255)
                                               ttAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAAtatta                                                                                                       <  2:211079/150‑1 (MQ=255)
                                               ttAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAAtatta                                                                                                       <  2:512235/150‑1 (MQ=255)
                                                tAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAAtattat                                                                                                      >  2:554590/1‑150 (MQ=255)
                                                 aaaCAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATg                                                                                                     >  1:208995/1‑150 (MQ=255)
                                                  aaCAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGc                                                                                                    <  1:923708/150‑1 (MQ=255)
                                                  aaCAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGc                                                                                                    <  1:1521909/150‑1 (MQ=255)
                                                   aCAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCa                                                                                                   >  2:2378270/1‑150 (MQ=255)
                                                   aCAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCa                                                                                                   >  1:1504726/1‑150 (MQ=255)
                                                    cAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaa                                                                                                  >  1:273968/1‑150 (MQ=255)
                                                    cAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCATGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaa                                                                                                  >  1:2000712/1‑150 (MQ=255)
                                                        tCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATc                                                                                              <  1:1434017/150‑1 (MQ=255)
                                                           acacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGca                                                                                           <  2:294692/150‑1 (MQ=255)
                                                           acacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGca                                                                                           >  1:1831517/1‑150 (MQ=255)
                                                            cacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcac                                                                                          >  2:474755/1‑150 (MQ=255)
                                                              ccAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcacca                                                                                        >  1:1763510/1‑150 (MQ=255)
                                                                aaTCGCCTGATCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACcaca                                                                                      >  2:1264039/1‑150 (MQ=255)
                                                                  tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGt                                                                                    <  2:1449173/150‑1 (MQ=255)
                                                                    gCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCa                                                                                  >  1:1255340/1‑150 (MQ=255)
                                                                     ccTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGtaaa                                                                                 >  1:2339966/1‑147 (MQ=255)
                                                                        gTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                                              <  2:168335/150‑1 (MQ=255)
                                                                        gTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                                              <  2:635937/150‑1 (MQ=255)
                                                                          tCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAtt                                                                            >  2:508341/1‑150 (MQ=255)
                                                                                 cACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGtttt                                                                     <  1:1478057/150‑1 (MQ=255)
                                                                                   cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTAc                                                                   <  1:727614/150‑1 (MQ=255)
                                                                                     gATGCTGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCTGCATTTTTCCAGGGTCAGAAAATCATAAATCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTActc                                                                 <  2:1347536/150‑1 (MQ=255)
                                                                                       tGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTg                                                               <  2:2443004/150‑1 (MQ=255)
                                                                                       tGCGGCGGCTGAATGCCGGTTGCGAGACGTTGAGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTg                                                               <  1:590712/150‑1 (MQ=255)
                                                                                         cggcggCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTc                                                             >  1:586363/1‑150 (MQ=255)
                                                                                         cggcggCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTc                                                             <  1:643955/150‑1 (MQ=255)
                                                                                            cggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgc                                                          <  1:2102866/150‑1 (MQ=255)
                                                                                            cggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgc                                                          >  1:675733/1‑150 (MQ=255)
                                                                                                 taaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                     <  1:1673378/149‑1 (MQ=255)
                                                                                                 gAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                     <  2:621889/150‑1 (MQ=255)
                                                                                                  aaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCt                                                    <  1:755003/150‑1 (MQ=255)
                                                                                                     gCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGc                                                 <  1:1021694/150‑1 (MQ=255)
                                                                                                        ggTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCggg                                              <  2:1615141/150‑1 (MQ=255)
                                                                                                        ggTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCggg                                              <  2:2043009/150‑1 (MQ=255)
                                                                                                           tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCgg                                               >  2:1371405/1‑146 (MQ=255)
                                                                                                           tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCgg                                               <  1:1371405/146‑1 (MQ=255)
                                                                                                           tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAt                                           <  1:338248/150‑1 (MQ=255)
                                                                                                            gCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAtt                                          >  2:669534/1‑150 (MQ=255)
                                                                                                             cGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTc                                         >  2:180375/1‑150 (MQ=255)
                                                                                                             cGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTc                                         >  1:266042/1‑150 (MQ=255)
                                                                                                             cGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTc                                         <  2:1054000/150‑1 (MQ=255)
                                                                                                               agaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTa                                       <  1:1196158/150‑1 (MQ=255)
                                                                                                               agaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTa                                       <  1:1664979/150‑1 (MQ=255)
                                                                                                                  cGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGt                                    >  2:1276364/1‑150 (MQ=255)
                                                                                                                   gTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTTCGGGCATTCTACGTc                                   <  2:2163078/150‑1 (MQ=255)
                                                                                                                        gactgactGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCGATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTc                              <  1:488532/150‑1 (MQ=255)
                                                                                                                         actgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCg                             <  2:998462/150‑1 (MQ=255)
                                                                                                                              ctgCCGCCTGGGAAAAATAGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcgg                        >  2:1928417/1‑150 (MQ=255)
                                                                                                                               tgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcggc                       <  2:273968/150‑1 (MQ=255)
                                                                                                                               tgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcggc                       <  2:746365/150‑1 (MQ=255)
                                                                                                                                gccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCCCCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCt                      >  2:1869149/1‑150 (MQ=255)
                                                                                                                                gccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCt                      <  2:2334145/150‑1 (MQ=255)
                                                                                                                                gccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCt                      >  1:1737730/1‑150 (MQ=255)
                                                                                                                                 tgaccctggAAAAATGCCGCAATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTg                     >  2:85905/8‑150 (MQ=255)
                                                                                                                                  gaccctggAAAAATGCCGCAATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGa                    >  1:875658/7‑150 (MQ=255)
                                                                                                                                   gccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGAc                   <  2:1778943/150‑1 (MQ=255)
                                                                                                                                     cTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACag                 <  1:1021619/150‑2 (MQ=255)
                                                                                                                                      ctggAAAAATGCCGCAATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAc                >  2:353972/3‑150 (MQ=255)
                                                                                                                                         gAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGt             >  2:2180818/1‑150 (MQ=255)
                                                                                                                                            aaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTa          <  1:508341/150‑1 (MQ=255)
                                                                                                                                            aaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTa          <  2:1255340/150‑1 (MQ=255)
                                                                                                                                                 gCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGCCAACCGTTTAAGCCg     >  2:17915/1‑150 (MQ=255)
                                                                                                                                                  cGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGt    >  1:1261739/1‑150 (MQ=255)
                                                                                                                                                  cGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGt    >  1:1626915/1‑150 (MQ=255)
                                                                                                                                                   ggCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt   >  1:1686267/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTT  >  NC_000913/4558067‑4558364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑