Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,606,118	G→A	8.2%	noncoding (48/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,118	0	G	A	8.2%	233.4 / 4.8	84	noncoding (48/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (26/49);  new base A (2/5);  total (29/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAAC  >  NC_000913/4605969‑4606247                                                                                                                                                      |                                                                                                                                  aTTTTGGTTCCATCTTTACTGGCAATTATTTCTGTAATCATTCTGATTAAAATTAATTTTAATCAGCTATGAAAAATCAAGCAACTAACAAGAAATTAATTAAAGTTATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                                                   <  2:2229780/150‑1 (MQ=16) aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                                                   >  1:484294/1‑150 (MQ=255) aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                                                   <  1:1947938/150‑1 (MQ=255)  ttttGCTACCATCGTGCCTAGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGa                                                                                                                                  >  1:2089334/1‑150 (MQ=255)   tttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                                                                 >  2:1324384/1‑150 (MQ=255)   tttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                                                                 <  1:1444290/150‑1 (MQ=255)   tttGCTACCATCGTGCCTGGCAATTATTGCTGGCATCATGCTGATGATAATTAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                                                                 <  2:2069200/150‑1 (MQ=255)     tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                                                                                               <  2:1484077/150‑1 (MQ=255)     tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                                                                                               >  2:370602/1‑150 (MQ=255)      gCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                                              <  2:1380383/150‑1 (MQ=255)       cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                                             >  1:1955463/1‑150 (MQ=255)       cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAAACCCCACGTCCGTAAGGACACTa                                                                                                                             >  1:93611/1‑150 (MQ=255)         aCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                                                                                                           <  1:2486249/150‑1 (MQ=255)          ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAACATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca                                                                                                                          <  1:370602/150‑1 (MQ=255)              cGTGCCTGCCACTTATTGCTGGAATCATGCGTATGAAAATTAATTTTAATCAGGGATGAAAAATCAAGTAATAAACAAGAAAGCAAGTAAACATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                                                                                     <  2:1942896/150‑1 (MQ=25)               gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                                                                                     >  1:1389462/1‑150 (MQ=255)               gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                                                                                     <  1:365531/150‑1 (MQ=255)                 gCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                                                   <  1:1483157/150‑1 (MQ=255)                 gCCTGGCACTTATTGCTGGCATCATGCTGATGAAAATGAATTTCAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGCAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                                                   <  1:1166940/150‑1 (MQ=255)                 gCCTAGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                                                   <  2:2089334/150‑1 (MQ=255)                 atcTGGCACTTATTGCTGGCATCATTCTGATGATAATTAATTTTAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAATTAAAGTTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                                                   <  2:1245134/148‑1 (MQ=37)                   cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAgat                                                                                                                 >  1:1245134/1‑148 (MQ=255)                   cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                                                 >  2:918268/1‑150 (MQ=255)                   cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                                                 <  1:2362065/150‑1 (MQ=255)                       ctttatttGCTTTCATCATGATGATCTTTATTATTTTTAATCAGGGATCAAAAATCAATGAAGAAACAAGATAGGAACTAAAGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                                             <  2:1261722/144‑1 (MQ=17)                       cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                                             >  2:1721442/1‑150 (MQ=255)                       cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                                             <  1:1369379/150‑1 (MQ=255)                         cTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                                           >  1:169920/1‑150 (MQ=255)                               gCTGGCATCATGCTTATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAACAAACAAGAAAGGAAGTAAAGATAATTTGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                     <  1:1548879/150‑1 (MQ=255)                               gCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                     >  1:791167/1‑150 (MQ=255)                               gCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                     <  2:1955463/150‑1 (MQ=255)                               gCTGCCATCATGCTGATGATAATGAATTTGAATCAGGGATCAAAAATCAAGCAACAAACAACAACGCAAGAAAACATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                     <  1:632558/150‑1 (MQ=21)                                cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                    >  1:2401820/1‑150 (MQ=255)                                cTGGCATCATGCTGAAGAAAAAGAATTGCAATCAGGGATCAAAAATCCAGCAACAAACAAGAAAGGAAGTAACGATAATTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTACGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                    <  2:2407637/150‑1 (MQ=21)                                  ggaatcatTCTGATGATAATTAATTTGAATCAGGGATGAAAAATCAAGGAATAAACAAGAAATTAATTAAAGATATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                                                  <  2:1692869/147‑1 (MQ=21)                                   gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCAGAAGCTAGCGCGTCTACCAAtt                                                                                                 >  1:1297492/1‑150 (MQ=255)                                      tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                              <  2:986180/150‑1 (MQ=255)                                       atgtcTGATTATTATGAATTTTAATCAGGGATGAAAAATCATGGAAGAAACAATAAATGAATTAAAGATATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                             <  2:1528155/146‑1 (MQ=18)                                        atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                            >  1:9733/1‑150 (MQ=255)                                         tGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                                                        <  1:1379698/137‑1 (MQ=255)                                         tGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                                                        >  2:1379698/1‑137 (MQ=255)                                         tGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                                           <  1:1357028/150‑1 (MQ=255)                                           cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCaccacc                                                                                         >  2:2457210/1‑150 (MQ=255)                                           cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcagag                                                                                         >  1:1528155/1‑146 (MQ=255)                                                tatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                    <  2:1497374/149‑1 (MQ=255)                                                 atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                                                   <  2:47379/150‑1 (MQ=255)                                                  tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcat                                                                                  >  2:2042700/1‑149 (MQ=255)                                                   aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcata                                                                                 >  1:1065820/1‑148 (MQ=255)                                                    aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcatac                                                                                >  2:1357986/1‑147 (MQ=255)                                                    aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACCTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTAACAATTACGCCACCTTAGcccac                                                                                >  2:2051612/1‑149 (MQ=21)                                                     ttaaTTTTAATCCGGGATGTAAACTCACGGAACAAACAATAATGGAACTAACGACAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                               <  1:2132933/148‑1 (MQ=14)                                                      taaTTTTAATCAGGGATGAAAAATCATGGAACTAACAATAAATGAATTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGtg                                                                              <  2:1677266/149‑2 (MQ=17)                                                       aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                                             <  2:1163485/150‑1 (MQ=255)                                                       aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                                             <  1:1713093/150‑1 (MQ=255)                                                        aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcataccata                                                                            >  2:2411803/1‑143 (MQ=255)                                                        aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAt                                                                            >  1:1923604/1‑150 (MQ=255)                                                             aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTcctagaaa                                                                       >  2:975098/1‑143 (MQ=255)                                                                    gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttttt                                                                <  1:1707616/150‑1 (MQ=255)                                                                    gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttttt                                                                <  1:468310/150‑1 (MQ=255)                                                                    gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCACCACCTTCGCACAGTCATCTTACttttttt                                                                <  1:2457210/150‑1 (MQ=255)                                                                     aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTg                                                               <  1:1283555/150‑1 (MQ=255)                                                                      tGAAAAATCAAGGAATAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGa                                                              <  2:1111869/150‑1 (MQ=255)                                                                              cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                                      <  1:1721442/150‑1 (MQ=255)                                                                                 ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCCTCTGACATATGAAGATATCCCCTctt                                                   >  1:1183817/1‑148 (MQ=17)                                                                                    agaaACAAGAAAGGTAGTAAAGATAATTGGTGCGATGGGGTGGACTTGAACCCCCACGTACGTAAGGACACTAACAGCTGAAGCTAGCTCGTGTAGGAATTCCGAGACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCGTCGtgtg                                                <  2:1180027/150‑1 (MQ=255)                                                                                     gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                    >  2:2452322/1‑97 (MQ=255)                                                                                     gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                    <  1:2452322/97‑1 (MQ=255)                                                                                      aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGatat                                                           >  2:1221141/1‑137 (MQ=255)                                                                                      aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGatat                                                           <  1:1221141/137‑1 (MQ=255)                                                                                      aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGt                                              <  1:1221068/150‑1 (MQ=255)                                                                                       aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                             <  1:129796/150‑1 (MQ=255)                                                                                           aGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAg                                         <  1:1068931/150‑1 (MQ=255)                                                                                           aGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAg                                         <  2:1923604/150‑1 (MQ=255)                                                                                                ggAAGTAAAGATAATTGGTGCGAGGGGGGTGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg                                    <  2:514056/150‑1 (MQ=255)                                                                                                ggAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg                                    <  1:273504/150‑1 (MQ=255)                                                                                                  taGTAATGATAATTGGTGCGAGGGTTGTGACTTGAACCCCCACGTACGTAAGGAGACTAACACCTGAAGCTAGCGAGTATACCTATTCCGCGACGTTCGCACAGTCATCTTACTTTTTTTGATATCTCCTCGTTTGGTGCGAgggggggg                                  <  2:9733/149‑1 (MQ=21)                                                                                                   aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAc                                <  2:77418/150‑1 (MQ=255)                                                                                                     cacacaTAATTGCGGCCAGGGGGGGTACTTGAACCCCCACGTCCGTAACGACACTAACACCTGAAGCTAGCGCGTCAACCAAATCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGTGCGAGGGGGGGGACt                               <  1:2060163/145‑1 (MQ=21)                                                                                                       aaGATACTTGGGGCGAGGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTg                             <  1:1487667/150‑1 (MQ=255)                                                                                                        aGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcatacc                                                                               >  1:524100/1‑95 (MQ=17)                                                                                                        aGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcatacc                                                                               <  2:524100/99‑5 (MQ=17)                                                                                                           tAATTGGTGCGAGTGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAATCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAcc                         <  1:909310/150‑1 (MQ=255)                                                                                                                  tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTg                                                >  1:967692/1‑120 (MQ=255)                                                                                                                  tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTg                                                <  2:967692/120‑1 (MQ=255)                                                                                                                       ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa             <  1:918268/150‑1 (MQ=255)                                                                                                                               aCTTGAACCGCCACGTCCTTAAGTACACTAACACCTGAAGCTACCTCTTATACCAATTCCGCCACCTTCTAACATTCATCTTACTTTTTTTGATCTCGCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt     <  2:1920677/150‑1 (MQ=25)                                                                                                                                  tGAACCCCCACGTGCGTAATGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCTCAGTCATCAAACATTTTTTGAATTCGCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAac  <  1:953006/150‑1 (MQ=21)                                                                                                                                                      |                                                                                                                                  ATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAAC  >  NC_000913/4605969‑4606247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑