Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 595,520 T→A 8.8% intergenic (‑77/‑80) cusR ← / → cusC DNA‑binding transcriptional activator CusR/copper/silver export system outer membrane channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913595,5200TA 8.8% 385.5 / 18.7 131intergenic (‑77/‑80)cusR/cusCDNA‑binding transcriptional activator CusR/copper/silver export system outer membrane channel
Reads supporting (aligned to +/- strand):  ref base T (65/54);  new base A (8/4);  total (73/58)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

AACCGGCTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCG  >  NC_000913/595371‑595665
                                                                                                                                                     |                                                                                                                                                 
aaCCGGCTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAtt                                                                                                                                                   <  1:957185/150‑1 (MQ=255)
 aCCGGCTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAttt                                                                                                                                                  <  2:747798/150‑1 (MQ=255)
 aCCGGCTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAttt                                                                                                                                                  >  2:1948115/1‑150 (MQ=255)
   cGGCTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCATCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTg                                                                                                                                                <  2:1313130/150‑1 (MQ=255)
      cTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCa                                                                                                                                             >  2:1713230/1‑150 (MQ=255)
         cGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCAttt                                                                                                                                          <  1:1534120/150‑1 (MQ=255)
           gTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGCCAATTTTGTCAttttt                                                                                                                                        >  2:2381733/1‑150 (MQ=255)
           gTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAAATTGTCAttttg                                                                                                                                        <  2:979411/150‑2 (MQ=255)
            ttAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTc                                                                                                                                       >  1:1559621/1‑150 (MQ=255)
            ttAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAAATTGTCAttttgc                                                                                                                                       <  1:851898/150‑3 (MQ=255)
              aaCCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTg                                                                                                                                     >  2:1531742/1‑150 (MQ=255)
               aCCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGt                                                                                                                                    >  1:1203109/1‑150 (MQ=255)
                cccTTTGGTCAAGTATTCTCCGCTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTCCCCGGGCAATTCTAGAGAACCGGGATCAGAACGCAATCGCTTATTGGCAAAATCCCAATTTTGTCATTTTTCTGTc                                                                                                                                   >  2:1540312/1‑150 (MQ=255)
                      ggTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGa                                                                                                                             >  2:972076/1‑150 (MQ=255)
                       gTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGaa                                                                                                                            >  1:96957/1‑150 (MQ=255)
                       gTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGaa                                                                                                                            >  2:379225/1‑150 (MQ=255)
                            gTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCa                                                                                                                       <  1:591224/150‑1 (MQ=255)
                              aTTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAATATCaga                                                                                                                     <  1:239118/150‑1 (MQ=255)
                                   ccGGTTTTCTTTTCATCTTCGAAAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTATAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTg                                                                                                                >  2:1865859/1‑150 (MQ=255)
                                    cGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTgg                                                                                                               >  2:651583/1‑150 (MQ=255)
                                      gTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTTCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCg                                                                                                             <  1:445457/150‑1 (MQ=255)
                                      gTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCg                                                                                                             <  2:1535554/150‑1 (MQ=255)
                                        tttcttttcATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAg                                                                                                           <  1:115327/150‑1 (MQ=255)
                                        tttcttttaATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAg                                                                                                           >  2:2354781/1‑150 (MQ=255)
                                           cttttcATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTaa                                                                                                        <  2:1335508/150‑1 (MQ=255)
                                            ttttcATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTaaa                                                                                                       >  2:278524/1‑150 (MQ=255)
                                            ttttcATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTaaa                                                                                                       >  2:1298218/1‑150 (MQ=255)
                                             tttcATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAg                                                                                                      >  1:373510/1‑150 (MQ=255)
                                                cATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTg                                                                                                   >  1:1535111/1‑150 (MQ=255)
                                                 aTCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTgg                                                                                                  >  1:469842/1‑150 (MQ=255)
                                                 aTCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAAATTTGTCATTTTTCTGTCCCCGGAAAATCAGCGCCTCGCGAGAAAAGTTgg                                                                                                  >  2:1881605/1‑150 (MQ=255)
                                                  tCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTggc                                                                                                 >  1:2045281/1‑150 (MQ=255)
                                                   cTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTTGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTgtcg                                                                                                <  2:1233468/150‑1 (MQ=255)
                                                   cTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTg                                                                                                   <  2:1107841/147‑1 (MQ=255)
                                                   cTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTg                                                                                                   >  1:1107841/1‑147 (MQ=255)
                                                     tCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTggcggc                                                                                              >  2:2370717/1‑150 (MQ=255)
                                                      cGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCa                                                                                             <  1:1492563/150‑1 (MQ=255)
                                                      cGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTATAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCa                                                                                             >  2:1713910/1‑150 (MQ=255)
                                                       gACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCAt                                                                                            >  1:1448883/1‑150 (MQ=255)
                                                           aTCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATaaaa                                                                                        >  1:2282324/1‑150 (MQ=255)
                                                             cAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATc                                                                                      >  1:1826879/1‑150 (MQ=255)
                                                             cAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATc                                                                                      >  2:771609/1‑150 (MQ=255)
                                                              aaCAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCa                                                                                     <  2:2387472/150‑1 (MQ=255)
                                                              aaCAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCa                                                                                     >  1:49606/1‑150 (MQ=255)
                                                              aaCAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCa                                                                                     <  1:126663/150‑1 (MQ=255)
                                                               aCAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCAc                                                                                    >  2:72079/1‑150 (MQ=255)
                                                                 aGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCa                                                                                  <  1:1801586/150‑1 (MQ=255)
                                                                    ttCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGaa                                                                               >  1:1297451/1‑150 (MQ=255)
                                                                      cATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAAt                                                                             >  2:1632757/1‑150 (MQ=255)
                                                                      cATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAAt                                                                             <  2:519702/150‑1 (MQ=255)
                                                                      cATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAAt                                                                             <  1:854655/150‑1 (MQ=255)
                                                                       atatTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAAtt                                                                            >  2:1696054/1‑150 (MQ=255)
                                                                         atTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTAt                                                                          <  1:1534797/150‑1 (MQ=255)
                                                                         atTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTAt                                                                          >  1:882707/1‑150 (MQ=255)
                                                                          tttCCTCCGCATGTTGCCCGGTCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGAAAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATATAATCACCAGAAATTATg                                                                         <  2:1203109/150‑1 (MQ=255)
                                                                              ctccGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGcc                                                                     <  2:1788669/150‑1 (MQ=255)
                                                                               tccGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCt                                                                    >  2:1395027/1‑150 (MQ=255)
                                                                               tccGCATGTTGCCCGGGCAATACTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCCGAAAATCAGAGCCTCGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCt                                                                    >  2:1415102/1‑150 (MQ=255)
                                                                                ccGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAA‑ATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTAt                                                                  <  1:693678/150‑1 (MQ=255)
                                                                                  gCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATg                                                                 <  1:1902823/150‑1 (MQ=255)
                                                                                  gCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATg                                                                 <  2:1776135/150‑1 (MQ=255)
                                                                                    aTGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGtc                                                               <  2:2080626/150‑1 (MQ=255)
                                                                                     tGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGtct                                                              <  1:367294/150‑1 (MQ=255)
                                                                                          cccGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTg                                                         <  1:1948115/150‑1 (MQ=255)
                                                                                            cGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTa                                                       <  2:1297451/150‑1 (MQ=255)
                                                                                             gggCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTaa                                                      <  2:2255007/150‑1 (MQ=255)
                                                                                              ggCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTaaa                                                     >  1:150292/1‑150 (MQ=255)
                                                                                              ggCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTaaa                                                     <  2:61173/150‑1 (MQ=255)
                                                                                                cAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACt                                                   >  1:1843626/1‑150 (MQ=255)
                                                                                                  aTTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                                 >  1:343031/1‑150 (MQ=255)
                                                                                                  aTTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                                 <  1:5775/150‑1 (MQ=255)
                                                                                                  aTTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTc                                                 >  1:7316/1‑150 (MQ=255)
                                                                                                    tCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTg                                               >  1:19133/1‑150 (MQ=255)
                                                                                                      tAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGcc                                             >  1:1056983/1‑150 (MQ=255)
                                                                                                          gTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCAttt                                         >  1:2256154/1‑150 (MQ=255)
                                                                                                          gTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATAAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCAttt                                         >  1:382406/1‑150 (MQ=255)
                                                                                                                ggATCAGATGGCAATCGCTTATTGGCAAAATGACATTTTTGTCATTTTTCTGTCACCGTAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTtttgtg                                   <  2:262729/150‑1 (MQ=255)
                                                                                                                  aTCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGc                                 <  1:836578/150‑1 (MQ=255)
                                                                                                                     aGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTGTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGACATTTTGTGTGGCCCt                              >  2:1627764/1‑150 (MQ=255)
                                                                                                                         ggCAATCGGTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTgcg                          <  2:354173/150‑1 (MQ=255)
                                                                                                                           cAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCt                        >  1:2055172/1‑150 (MQ=255)
                                                                                                                             aTCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTaa                      >  1:1576439/1‑150 (MQ=255)
                                                                                                                               cGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAAcc                    >  1:2249761/1‑150 (MQ=255)
                                                                                                                                gCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCg                   >  1:257710/1‑150 (MQ=255)
                                                                                                                                gCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCg                   >  1:1475180/1‑150 (MQ=255)
                                                                                                                                  ttATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGgt                 >  2:1768867/1‑150 (MQ=255)
                                                                                                                                   tATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGgtt                >  2:1920543/1‑150 (MQ=255)
                                                                                                                                      cagaaaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGgttgtt             >  2:1060128/5‑150 (MQ=255)
                                                                                                                                      cagaaaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGATAACCGgttgtt             >  2:2469213/5‑150 (MQ=255)
                                                                                                                                       ggCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTc            >  1:247015/1‑150 (MQ=255)
                                                                                                                                       ggCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTc            <  2:373510/150‑1 (MQ=255)
                                                                                                                                       agaaaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTc            >  2:1590184/4‑150 (MQ=255)
                                                                                                                                       agaaaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTc            >  1:1465713/4‑150 (MQ=255)
                                                                                                                                        gaaaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCGTGGCGAGTAAAGTAGGCGGCATAAAATCACCAGAAATTATGACCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACAGGTTGTTcc           >  2:1215270/3‑149 (MQ=255)
                                                                                                                                         cAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCAc          >  1:1678867/1‑150 (MQ=255)
                                                                                                                                         cAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTAGGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCAc          >  2:1939287/1‑150 (MQ=255)
                                                                                                                                          aaaaTGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACt         >  1:2107615/1‑150 (MQ=255)
                                                                                                                                          aaaaTGACAAAATTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACt         >  1:151112/1‑150 (MQ=255)
                                                                                                                                            aaTGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTgg       <  2:2340387/150‑1 (MQ=255)
                                                                                                                                               gACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCAc    >  1:664077/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AACCGGCTTCGGTTAACCCTTTGGTCAAGTATTCTCCGGTTTTCTTTTCATCTTCGACAATCAACAGTTTCATATTTCCTCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGCAAAATGACAATTTTGTCATTTTTCTGTCACCGGAAAATCAGAGCCTGGCGAGTAAAGTTGGCGGCATAAAATCACCAGAAATTATGAGCCTATGTCTCCTTGTAAACTTCTGCCATTTTGTGTGGCCCTTGCGCTAACCGGTTGTTCACTGGCACCG  >  NC_000913/595371‑595665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑