Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 753,634 T→A 10.4% I279F (ATC→TTC)  gltA ← citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,6340TA10.4% 446.3 / 30.1 153I279F (ATC→TTC) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (64/73);  new base A (5/11);  total (69/84)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 157 total aligned reads displayed.

TTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGG  >  NC_000913/753485‑753782
                                                                                                                                                     |                                                                                                                                                    
tttCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAt                                                                                                                                                      <  1:54529/150‑1 (MQ=255)
  tCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAttt                                                                                                                                                    <  2:1000444/150‑1 (MQ=255)
     ccACTTCATTGCATGGTTCACGCATTACCGTGGCGCGCGGGTCGTAATTTTTGTACCCGCGGTGACCGAATCCCATCAGGCTGAAAGAATCATTTTTGTGTTTAGCAAGACGAACAAATTCCGGAATCTGTTTAAAGGATCTGATttctt                                                                                                                                                 <  2:2041999/149‑1 (MQ=255)
       aCTTCATTGCATGTTTCAAGCATTACGGTGGCGCGCGGGGCGTAATTTTTGTACACGCGGTTACCGAATCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTcc                                                                                                                                               <  2:1110339/150‑1 (MQ=255)
       aCTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTcc                                                                                                                                               <  1:2373002/150‑1 (MQ=255)
        cTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCa                                                                                                                                              >  1:28912/1‑150 (MQ=255)
         ttCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAg                                                                                                                                             <  2:544644/150‑1 (MQ=255)
          tCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGc                                                                                                                                            >  2:1726858/1‑150 (MQ=255)
             tgacAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCAtt                                                                                                                                         >  1:1161936/4‑150 (MQ=255)
             tGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCAtt                                                                                                                                         >  2:1744662/1‑150 (MQ=255)
                 aGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCa                                                                                                                                     <  1:837321/150‑1 (MQ=255)
                  ggTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGGGCTGATTTCTTCCAGCATTTTCAg                                                                                                                                    >  1:147115/1‑150 (MQ=255)
                   gTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAgc                                                                                                                                   <  1:1379685/150‑1 (MQ=255)
                     ttCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAAGGGAGCTGATTTCTTCCAGCATTTTCAgcgc                                                                                                                                 >  2:1561217/1‑150 (MQ=255)
                      tCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGcgcc                                                                                                                                >  1:2321399/1‑150 (MQ=255)
                         cGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCt                                                                                                                             >  1:1212995/1‑150 (MQ=255)
                          gCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCtt                                                                                                                            >  1:382184/1‑150 (MQ=255)
                           cATTACGGTGGCTGGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGGCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTGCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTc                                                                                                                           <  2:2103783/150‑1 (MQ=255)
                           cATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCACATAAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTc                                                                                                                           >  2:309393/1‑150 (MQ=255)
                            aTTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCg                                                                                                                          <  1:1357590/150‑1 (MQ=255)
                              tACGGTGGCGCGCGGGTCTTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGtt                                                                                                                        <  1:2410483/150‑1 (MQ=255)
                              tACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGtt                                                                                                                        >  1:1298117/1‑150 (MQ=255)
                               aCGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTa                                                                                                                       <  2:855467/150‑1 (MQ=255)
                                      cgcgcgGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCAccgc                                                                                                                >  2:711490/1‑150 (MQ=255)
                                      cgcgcgGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCAccgc                                                                                                                >  1:2255953/1‑150 (MQ=255)
                                             gTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATttc                                                                                                                                                   >  2:633731/1‑108 (MQ=255)
                                             gTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATttc                                                                                                                                                   <  1:633731/108‑1 (MQ=255)
                                              tCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCa                                                                                                        <  1:345243/150‑1 (MQ=255)
                                              tCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCa                                                                                                        <  2:2263030/150‑1 (MQ=255)
                                               cGTAATTTTTGTACACGCGGAGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTATTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAg                                                                                                       >  2:1296249/1‑150 (MQ=255)
                                                gTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGGTTATTTCTTCCAGCATTTTCAGCGCCGGTTCGTTAGCACCGCCGTGCGCAgg                                                                                                      <  2:288307/150‑1 (MQ=255)
                                                gTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAgg                                                                                                      >  2:2235643/1‑150 (MQ=255)
                                                gTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAgg                                                                                                      >  1:2448491/1‑150 (MQ=255)
                                                 tAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGt                                                                                                     >  2:649566/1‑150 (MQ=255)
                                                    tttttGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTccc                                                                                                  <  2:883386/150‑1 (MQ=255)
                                                          tACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCCTTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGt                                                                                            >  1:982542/1‑150 (MQ=255)
                                                          tACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGt                                                                                            >  2:105737/1‑150 (MQ=255)
                                                          tACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGt                                                                                            <  2:1138690/150‑1 (MQ=255)
                                                            cacGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGa                                                                                          <  1:802846/150‑1 (MQ=255)
                                                              cgcgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAg                                                                                        <  1:2261357/150‑1 (MQ=255)
                                                              cgcgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAg                                                                                        >  2:1857055/1‑150 (MQ=255)
                                                              cgcgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAg                                                                                        <  2:1572560/150‑1 (MQ=255)
                                                               gcgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGc                                                                                       <  2:1229553/150‑1 (MQ=255)
                                                               gcgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGc                                                                                       <  2:2321399/150‑1 (MQ=255)
                                                                cgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCa                                                                                      >  2:171758/1‑150 (MQ=255)
                                                                cgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCa                                                                                      <  2:1387628/150‑1 (MQ=255)
                                                                cgGTGACCGAAGCCCAACAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCa                                                                                      >  2:1507589/1‑150 (MQ=255)
                                                                  gTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAAt                                                                                    >  1:1522980/1‑150 (MQ=255)
                                                                   tGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATa                                                                                   <  2:718391/150‑1 (MQ=255)
                                                                      ccGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACct                                                                                >  1:1331897/1‑150 (MQ=255)
                                                                       cGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAACTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACctg                                                                               >  1:1397918/1‑150 (MQ=255)
                                                                        gAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACctgc                                                                              >  1:77263/1‑150 (MQ=255)
                                                                            cccATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCg                                                                          >  1:398695/1‑150 (MQ=255)
                                                                             ccATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGa                                                                         >  2:1322908/1‑150 (MQ=255)
                                                                              cATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGGTTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGAt                                                                        <  2:2477148/150‑1 (MQ=255)
                                                                              cATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGAt                                                                        <  1:1662379/150‑1 (MQ=255)
                                                                                tCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATAc                                                                      <  1:444356/150‑1 (MQ=255)
                                                                                 cAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGc                                                                                       <  1:274761/132‑1 (MQ=255)
                                                                                 cAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGc                                                                                       >  2:274761/1‑132 (MQ=255)
                                                                                  aGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAg                                                                    <  1:1741051/150‑1 (MQ=255)
                                                                                   ggCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                   <  1:21712/150‑1 (MQ=255)
                                                                                   ggCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                   <  2:2107669/150‑1 (MQ=255)
                                                                                   ggCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                   <  1:76663/150‑1 (MQ=255)
                                                                                   ggCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                   <  1:309393/150‑1 (MQ=255)
                                                                                    gCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGc                                                                  >  2:809878/1‑150 (MQ=255)
                                                                                         aaGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACg                                                             <  2:1213258/150‑1 (MQ=255)
                                                                                              tCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTc                                                        <  1:17746/150‑1 (MQ=255)
                                                                                              tCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTc                                                        <  2:1456801/150‑1 (MQ=255)
                                                                                               cATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCg                                                       <  1:568750/150‑1 (MQ=255)
                                                                                               cATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCg                                                       <  1:2472279/150‑1 (MQ=255)
                                                                                                aTTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGc                                                      <  1:904090/150‑1 (MQ=255)
                                                                                                 tttttGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCa                                                     >  1:805931/1‑150 (MQ=255)
                                                                                                   tttGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCAcc                                                   <  1:1442567/150‑1 (MQ=255)
                                                                                                   tttGTCTTTCGCACGACGAACAAACTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCAcc                                                   <  2:1397918/150‑1 (MQ=255)
                                                                                                    ttGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCAccc                                                  <  2:418346/150‑1 (MQ=255)
                                                                                                     tGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCg                                                 >  2:1486370/1‑150 (MQ=255)
                                                                                                      gTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGa                                                >  1:682528/1‑150 (MQ=255)
                                                                                                       tCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGaa                                               <  1:611201/150‑1 (MQ=255)
                                                                                                        cTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAag                                              >  1:1892210/1‑150 (MQ=255)
                                                                                                          ttCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAagag                                            <  1:1857055/150‑1 (MQ=255)
                                                                                                            cGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGcc                                          >  1:1825129/1‑150 (MQ=255)
                                                                                                            cGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGcc                                          >  1:1833823/1‑150 (MQ=255)
                                                                                                              cACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAg                                        >  2:1880928/1‑150 (MQ=255)
                                                                                                               acgacgAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGc                                       <  2:398695/150‑1 (MQ=255)
                                                                                                                cgacgaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCg                                      >  1:23421/1‑150 (MQ=255)
                                                                                                                     aaCAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCATGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCg                                                                          >  2:839939/1‑109 (MQ=255)
                                                                                                                     aaCAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCATGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCg                                                                          <  1:839939/109‑1 (MQ=255)
                                                                                                                                 aaTGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGt                     >  1:1214224/1‑150 (MQ=255)
                                                                                                                                    gtgtTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTaga                  <  2:542305/150‑1 (MQ=255)
                                                                                                                                      gtTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAgg                <  1:1726858/150‑1 (MQ=255)
                                                                                                                                      gtTTAACGGAGCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAgg                <  2:1255619/150‑1 (MQ=255)
                                                                                                                                           aCGGAGCTGATTTCTTCCAGTATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGGCCCCACAGTGAAGCAATACCTGCTGCGATACTGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTc           <  1:1817048/150‑1 (MQ=255)
                                                                                                                                           aCGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTc           <  2:1331897/150‑1 (MQ=255)
                                                                                                                                              gAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGt        >  2:592749/1‑150 (MQ=255)
                                                                                                                                              gAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGt        <  1:105737/150‑1 (MQ=255)
                                                                                                                                                gCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTc      >  2:2145998/1‑150 (MQ=255)
                                                                                                                                                gCTGAATTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTc      <  2:2063441/150‑1 (MQ=255)
                                                                                                                                                 cTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCa     <  2:767049/150‑1 (MQ=255)
                                                                                                                                                  tGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCAt    >  1:2316058/1‑150 (MQ=255)
                                                                                                                                                    aTTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATgg  >  1:1962499/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGG  >  NC_000913/753485‑753782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑