Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 869,378 T→G 5.2% F620C (TTC→TGC) ‡ gsiA → glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913869,3780TG 5.2% 465.0 / 11.5 151F620C (TTC→TGC) ‡gsiAglutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand):  ref base T (69/73);  new base G (2/6);  total (72/79)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

GAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTCACC  >  NC_000913/869230‑869527
                                                                                                                                                    |                                                                                                                                                     
gAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTc                                                                                                                                                      >  1:2366797/1‑150 (MQ=255)
     gTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCg                                                                                                                                                 <  1:1751929/150‑1 (MQ=255)
     gTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCg                                                                                                                                                 <  1:872460/150‑1 (MQ=255)
       cccGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTa                                                                                                                                               <  1:907203/150‑1 (MQ=255)
       cccGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTa                                                                                                                                               <  1:2194600/150‑1 (MQ=255)
        ccGACAACGACCGCAGCGTGTACTGCTGTCGGCCGATCTTCCAAGCAATATTCATCTGCGGGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACCACCACAATCAGAATACGCATTCATGCGTAg                                                                                                                                              >  1:1836258/1‑150 (MQ=255)
          gACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATCACGTCGCACAACCACAATCAGAATACGCATGAATGCGTattc                                                                                                                                            <  2:479546/150‑4 (MQ=255)
           aCAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATGAATGCGTattct                                                                                                                                           <  2:911749/150‑5 (MQ=255)
             aaCGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAAc                                                                                                                                         <  2:2470776/150‑1 (MQ=255)
              aCGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCGATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATGAATGCGTattctgat                                                                                                                                        <  2:1166164/150‑8 (MQ=255)
              aCGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCTTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATTCGCATGATTGCGTattctgat                                                                                                                                        <  2:681424/150‑8 (MQ=255)
              aCGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACa                                                                                                                                        >  1:2312039/1‑150 (MQ=255)
                 aCCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTc                                                                                                                                     <  1:1414704/150‑1 (MQ=255)
                  ccGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCa                                                                                                                                    >  1:2426600/1‑150 (MQ=255)
                    gCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAgg                                                                                                                                  >  2:2160378/1‑150 (MQ=255)
                       gCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCgg                                                                                                                               >  1:222641/1‑150 (MQ=255)
                        cGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGga                                                                                                                              <  2:1267109/150‑1 (MQ=255)
                        cGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGga                                                                                                                              <  2:50068/150‑1 (MQ=255)
                         gtgtACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGgag                                                                                                                             <  1:524220/150‑1 (MQ=255)
                         gtgtACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGgag                                                                                                                             <  1:2257726/150‑1 (MQ=255)
                          tgtACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGgaga                                                                                                                            <  2:1639833/150‑1 (MQ=255)
                           gtACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGaa                                                                                                                           >  1:1423777/1‑150 (MQ=255)
                                 ctgTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAAt                                                                                                                     <  2:1623718/150‑1 (MQ=255)
                                 ctgTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAAt                                                                                                                     <  1:1449308/150‑1 (MQ=255)
                                     cGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCa                                                                                                                 <  1:32478/150‑1 (MQ=255)
                                          gATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGc                                                                                                            <  2:922264/150‑1 (MQ=255)
                                           atcatcCCACCAATATTCCTCTGCCTGGCGAACCGGTCGCAGCCGTCTCGTTGCAACGAGGCGGGCCGGGGCAATCCGACGCACAACCACAATCCCAATCCGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAACAGCt                                                                                                           <  2:2206426/150‑1 (MQ=255)
                                              ttCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTa                                                                                                        >  1:2378512/1‑150 (MQ=255)
                                              ttCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGAGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATAAGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGATGTa                                                                                                        >  2:2286307/1‑150 (MQ=255)
                                                cccAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGGCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTacc                                                                                                      >  2:524115/1‑149 (MQ=255)
                                                 ccAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTacac                                                                                                     >  1:3419/1‑150 (MQ=255)
                                                  cAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACAcc                                                                                                    >  1:884180/1‑150 (MQ=255)
                                                  cAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACAcc                                                                                                    >  1:1732524/1‑150 (MQ=255)
                                                   aGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCg                                                                                                   >  1:1945746/1‑150 (MQ=255)
                                                    gCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTAGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGt                                                                                                  >  2:1753676/1‑150 (MQ=255)
                                                      aaTATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAg                                                                                                <  2:2366797/150‑1 (MQ=255)
                                                      aaTATTCATCTGCATGGAGAAGAGGTGGCAGACTTCTCGTAGCAATGCGTCGGGCAGGTGCATTAAGTAGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAg                                                                                                >  2:1003071/1‑150 (MQ=255)
                                                          ttCATCTGCGTTGCGAAGAGGTGCCAGGCGTCTCGTTGCACTGCGTCGGGCCGGGGCATTACTTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCACTCGGATAATAAAATGGCAAGAGCTGTACACCGTAGTggg                                                                                            <  2:1841016/150‑1 (MQ=255)
                                                              tCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAg                                                                                        <  2:882788/150‑1 (MQ=255)
                                                                tGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTg                                                                                      >  2:2217926/1‑150 (MQ=255)
                                                                tGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTg                                                                                      <  1:296106/150‑1 (MQ=255)
                                                                   gTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGgcg                                                                                   >  2:2061451/1‑150 (MQ=255)
                                                                    tGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGgcgc                                                                                  >  2:1967228/1‑150 (MQ=255)
                                                                      gCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCATAGTGGGTTAGTGGCGCTg                                                                                <  1:1291177/150‑1 (MQ=255)
                                                                       cGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTgg                                                                               <  1:720964/150‑1 (MQ=255)
                                                                        gAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTggg                                                                              >  1:1517618/1‑150 (MQ=255)
                                                                        gAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTggg                                                                              <  1:1149411/150‑1 (MQ=255)
                                                                            agGTGGCCGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATAACGTCGCACAACCACACTCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGCGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCAtt                                                                          >  2:1436526/1‑150 (MQ=255)
                                                                             ggtcccAGCCCTCTCGTTGCAATGCGACGGGCAGGGGCATTACGTCGCACAACCACAAACAGAATAAGCAAACATGCGTAGAAAACATTCAGCCGGAGAATAAAATGGCAAGAGGGGAACACCCTACTGGGTTAGAGGCGCTGGGCATTg                                                                         >  2:2371085/6‑150 (MQ=255)
                                                                             ggTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTg                                                                         >  2:1508706/1‑150 (MQ=255)
                                                                             ggTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTAGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGATGTACACCGTAGTGGGTTAGTGGAGATGGGCATTg                                                                         >  2:2362485/1‑150 (MQ=255)
                                                                               tGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCg                                                                       <  1:467442/150‑1 (MQ=255)
                                                                               tGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCg                                                                       >  2:570390/1‑150 (MQ=255)
                                                                               tGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGGAGTGGGTTAGTGGCGCTGGGCATTGCg                                                                       >  1:104764/1‑150 (MQ=255)
                                                                                 gCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGAc                                                                     <  2:1641438/150‑1 (MQ=255)
                                                                                 gCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGAc                                                                     <  1:1158232/150‑1 (MQ=255)
                                                                                   aGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAg                                                                   <  1:2160378/150‑1 (MQ=255)
                                                                                    gCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGc                                                                  >  2:1346757/1‑150 (MQ=255)
                                                                                     ccGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCg                                                                 <  1:2033185/150‑1 (MQ=255)
                                                                                     ccGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCg                                                                 <  2:369749/150‑1 (MQ=255)
                                                                                       gTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGCCGCTGGGCATTGCGACAGCGtt                                                               >  2:1859837/1‑150 (MQ=255)
                                                                                           cGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATg                                                           >  1:1095568/1‑150 (MQ=255)
                                                                                            gTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATgg                                                          <  1:1508706/150‑1 (MQ=255)
                                                                                             ttGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGc                                                         >  1:1021242/1‑150 (MQ=255)
                                                                                               gCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCAt                                                       >  1:1177631/1‑150 (MQ=255)
                                                                                                 aaTGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCt                                                     <  2:1732524/150‑1 (MQ=255)
                                                                                                     cGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGTCGGAGAATAATATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTtgtg                                                 <  2:1342561/150‑1 (MQ=255)
                                                                                                     cGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTtgtg                                                 <  1:1460116/150‑1 (MQ=255)
                                                                                                     cGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTtgtg                                                 <  2:761823/150‑1 (MQ=255)
                                                                                                      gTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGc                                                >  2:465385/1‑150 (MQ=255)
                                                                                                         gggCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCAtt                                             <  1:538307/150‑1 (MQ=255)
                                                                                                          ggCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTc                                            <  2:222641/150‑1 (MQ=255)
                                                                                                           gCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCg                                           >  2:579552/1‑150 (MQ=255)
                                                                                                            ccGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGc                                          <  2:251282/150‑1 (MQ=255)
                                                                                                            ccGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGc                                          <  2:1220876/150‑1 (MQ=255)
                                                                                                              ggggCATTACATCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTg                                        <  1:2406340/150‑1 (MQ=255)
                                                                                                               gggCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGc                                       <  2:2185668/150‑1 (MQ=255)
                                                                                                                  cATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCaa                                    >  1:1679366/1‑150 (MQ=255)
                                                                                                                   aTTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCaaa                                   >  1:1687679/1‑150 (MQ=255)
                                                                                                                    ttACGTCGCACAACCACTATCAGATTACGCATTCATGCGTAGATAACATTCAGTCGGAGAATAAAATGGCAAGAGCTGTACACCGTTGTGGGTTAGTGGCGCTGGGGATTGCGACAGCGTTGATGGCTTCTTGTGCATTCGCTGCCAAAg                                  <  2:2249378/150‑1 (MQ=255)
                                                                                                                    ttACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAg                                  <  1:1877533/150‑1 (MQ=255)
                                                                                                                          cGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATgtgg                            <  1:475672/150‑1 (MQ=255)
                                                                                                                           gCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATgtggt                           <  1:2077933/150‑1 (MQ=255)
                                                                                                                            cacaACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATgtggtg                          >  1:1618524/1‑150 (MQ=255)
                                                                                                                                aCCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGATTAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGtggtggtgg                      >  2:1963819/1‑150 (MQ=255)
                                                                                                                                aCCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGtggtggtgg                      >  2:211228/1‑150 (MQ=255)
                                                                                                                                   acaATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAATATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCgg                   <  2:1533641/150‑1 (MQ=255)
                                                                                                                                   acaATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCgg                   <  1:1753676/150‑1 (MQ=255)
                                                                                                                                    caATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGATAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGt                  <  2:510827/150‑1 (MQ=255)
                                                                                                                                    caATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGt                  >  1:825555/1‑150 (MQ=255)
                                                                                                                                      aTCAGAATACGAATTCATGCGTAGATAACAGTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGGAGTGGGTTAGTGGCGCTGGGCATTGCGCCAGCGGTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAg                >  1:700733/1‑150 (MQ=255)
                                                                                                                                         tatctACGCATGAATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGAt             >  1:544141/5‑150 (MQ=255)
                                                                                                                                           tctACGCATGAATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCg           >  2:1481754/3‑150 (MQ=255)
                                                                                                                                             tACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGGAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGaa         >  2:366495/1‑150 (MQ=255)
                                                                                                                                               cGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAAtt       <  1:528586/150‑1 (MQ=255)
                                                                                                                                                 cATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGAGAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTc     <  1:1097236/150‑1 (MQ=255)
                                                                                                                                                 cATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTc     >  2:2310470/1‑150 (MQ=255)
                                                                                                                                                  aTTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTca    <  1:80457/150‑1 (MQ=255)
                                                                                                                                                   ttCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTcac   <  1:1630428/150‑1 (MQ=255)
                                                                                                                                                    tCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTcacc  <  1:229241/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTCACC  >  NC_000913/869230‑869527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑