Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 933,155 T→C 5.1% intergenic (+66/‑69) lrp → / → ftsK DNA‑binding transcriptional dual regulator Lrp/cell division DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913933,1550TC 5.1% 485.1 / 10.9 156intergenic (+66/‑69)lrp/ftsKDNA‑binding transcriptional dual regulator Lrp/cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base T (76/71);  new base C (3/5);  total (79/77)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 156 total aligned reads displayed.

TGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTT  >  NC_000913/933007‑933300
                                                                                                                                                    |                                                                                                                                                 
tGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTg                                                                                                                                                  <  2:1403431/150‑1 (MQ=255)
  ccTGGCGGCAACGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGGCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTggg                                                                                                                                                <  2:296053/150‑1 (MQ=255)
    tGGCGTCAATGACACACGGACATACCTTGTTATGGAAGAAGTCAAGCAGAGTAATAGTCTGGTTATTAAGACGCGCTAACACGGAAAAGGAGCAAAATCGGCGTAATTACATTACACTCATGTTAATCCATACAGCAACAGTACTGGGGt                                                                                                                                              >  2:2109743/1‑150 (MQ=255)
       cGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAAc                                                                                                                                           >  1:809354/1‑150 (MQ=255)
          cAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTg                                                                                                                                        >  2:1804893/1‑150 (MQ=255)
           actGACAGACGGACATACGGTGTTATGGAAGACGTCAAGCAGAGTAATCGTCTGGGTAGTAAGACGGGCTACCACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTgg                                                                                                                                       <  2:1568731/148‑1 (MQ=255)
           aaTGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTgg                                                                                                                                       >  2:1279075/1‑150 (MQ=255)
           aaTGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTgg                                                                                                                                       <  2:2376356/150‑1 (MQ=255)
              gACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTAc                                                                                                                                    <  2:568092/150‑1 (MQ=255)
                 acacGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTTACCCCAGTACtgt                                                                                                                                 <  1:415012/150‑1 (MQ=255)
                  cacGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACtgtt                                                                                                                                >  1:347653/1‑150 (MQ=255)
                   acGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTACCCCAGTACtgttgc                                                                                                                              <  2:1427339/150‑2 (MQ=255)
                    cGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTTCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTTACCCCAGTACtgttgc                                                                                                                              <  1:313499/150‑2 (MQ=255)
                    cGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACtgttgt                                                                                                                              <  2:2420331/150‑1 (MQ=255)
                    cGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACCAGGTTACCCCAGTACtgttgc                                                                                                                              <  1:731894/150‑2 (MQ=255)
                     ggACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTc                                                                                                                             <  1:1775393/150‑1 (MQ=255)
                       aCATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCg                                                                                                                           <  1:1981215/150‑1 (MQ=255)
                       aCATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCg                                                                                                                           <  2:1005376/150‑1 (MQ=255)
                         aTACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGtt                                                                                                                         <  2:64641/150‑1 (MQ=255)
                          tACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGttt                                                                                                                        >  1:2109428/1‑150 (MQ=255)
                          tACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGttt                                                                                                                        <  1:2179829/150‑1 (MQ=255)
                           aCGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGtttt                                                                                                                       <  1:2350083/150‑1 (MQ=255)
                            cGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTa                                                                                                                      <  1:1937645/150‑1 (MQ=255)
                            cGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTa                                                                                                                      >  1:2145206/1‑150 (MQ=255)
                             gttgttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTTGTACTGTTGTCCGTTTTAg                                                                                                                     <  1:454315/150‑1 (MQ=255)
                             gttgttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAg                                                                                                                     <  1:1804893/150‑1 (MQ=255)
                                gttATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCAt                                                                                                                  >  1:1816782/1‑150 (MQ=255)
                                    tGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCggg                                                                                                              <  1:574756/150‑1 (MQ=255)
                                    tGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCggg                                                                                                              >  2:87964/1‑150 (MQ=255)
                                      gaagaaGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCa                                                                                                            >  2:1130195/1‑150 (MQ=255)
                                       aagaagTCAAGCAGATTAATCGTCTGGTTATTAAGACGCGCTAAGACGTAACAGGTTCAAAATCGGCGTATTTTTATTACACTCCTGTTAATCCATACAGCAACAGTACTTGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAg                                                                                                           <  2:2452890/150‑1 (MQ=255)
                                       aagaagGCAAGGAGAGTTATCGTCTGGTTATTAAGACGCGCTAACACGTAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGTGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAg                                                                                                           <  2:1152706/150‑1 (MQ=255)
                                        agaagTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGAAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAgg                                                                                                          >  1:2099760/1‑150 (MQ=255)
                                            gTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGaaaa                                                                                                      >  1:1466742/1‑150 (MQ=255)
                                            gTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGaaaa                                                                                                      >  2:379314/1‑150 (MQ=255)
                                            gTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGaaaa                                                                                                      >  1:19796/1‑150 (MQ=255)
                                               aaGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGTAAAAGcc                                                                                                   <  1:1667915/150‑1 (MQ=255)
                                               aaGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGcc                                                                                                   >  1:1157387/1‑150 (MQ=255)
                                               aaGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGcc                                                                                                   <  2:1133057/150‑1 (MQ=255)
                                                aGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCt                                                                                                  >  2:656625/1‑150 (MQ=255)
                                                aGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCt                                                                                                  >  2:711607/1‑150 (MQ=255)
                                                    gagTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTaa                                                                                              >  2:1740219/1‑150 (MQ=255)
                                                    gagTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTaa                                                                                              >  2:1028644/1‑150 (MQ=255)
                                                         aTCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTTCACTCGTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTgg                                                                                         <  1:1001975/150‑1 (MQ=255)
                                                         aTCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTgg                                                                                         >  2:2244052/1‑150 (MQ=255)
                                                           cGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGgag                                                                                       <  1:12903/150‑1 (MQ=255)
                                                            gTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGgaga                                                                                      <  2:337721/150‑1 (MQ=255)
                                                             tCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGagag                                                                                     >  2:993628/1‑150 (MQ=255)
                                                                     ttaAGACGCGCTAACACGGATCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCtt                                                                             <  1:2193602/150‑1 (MQ=255)
                                                                     ttaAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCtt                                                                             >  1:347811/1‑150 (MQ=255)
                                                                      taAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTg                                                                            >  2:1336034/1‑150 (MQ=255)
                                                                       aaGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGa                                                                           <  2:475349/150‑1 (MQ=255)
                                                                        aGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAg                                                                          >  1:760288/1‑150 (MQ=255)
                                                                         gACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCAAACAGCAACAGTACTCGGGTAACCTCGTACTGTTCTCCGTTTTAGCATCGGGCAGGAAAAGCCTCTATCCTCCAGAGCCATTCTTGatc                                                                         >  2:2291145/1‑148 (MQ=255)
                                                                               cTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGaa                                                                   >  2:1621073/1‑150 (MQ=255)
                                                                                tAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAAt                                                                  <  1:479848/150‑1 (MQ=255)
                                                                                 aaCACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATa                                                                 >  1:575537/1‑150 (MQ=255)
                                                                                   cacGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACa                                                               >  1:1231011/1‑150 (MQ=255)
                                                                                         aCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAg                                                         <  1:1130195/150‑1 (MQ=255)
                                                                                         aCAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAg                                                         <  1:617004/150‑1 (MQ=255)
                                                                                            ggTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACa                                                      <  2:216702/150‑1 (MQ=255)
                                                                                             gTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACaa                                                     >  1:1500582/1‑150 (MQ=255)
                                                                                              tGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACaaa                                                    >  2:2307623/1‑150 (MQ=255)
                                                                                               gCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAaag                                                   <  1:1279075/150‑1 (MQ=255)
                                                                                                cAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAaaga                                                  >  2:553659/1‑150 (MQ=255)
                                                                                                cAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAaaga                                                  >  2:2009745/1‑150 (MQ=255)
                                                                                                  aaaTCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAaagaag                                                <  1:1587433/150‑1 (MQ=255)
                                                                                                    aTCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTc                                              <  2:809354/150‑1 (MQ=255)
                                                                                                      cGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTcac                                            <  1:537763/150‑1 (MQ=255)
                                                                                                       ggCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTcaca                                           >  1:2053738/1‑150 (MQ=255)
                                                                                                         cGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACAtt                                         >  1:588801/1‑150 (MQ=255)
                                                                                                         cGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACAtt                                         >  2:952028/1‑150 (MQ=255)
                                                                                                          gTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTg                                        <  2:2099760/150‑1 (MQ=255)
                                                                                                            aTTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGAc                                      >  1:2199630/1‑150 (MQ=255)
                                                                                                            aTTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGAc                                      <  1:2408843/150‑1 (MQ=255)
                                                                                                              tttGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACaa                                    >  1:971977/1‑150 (MQ=255)
                                                                                                                   ttACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAATGCCTGTAACCTGCAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAATTCACATTGACAAAGTTa                               <  2:799335/150‑1 (MQ=255)
                                                                                                                     acacTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAg                             <  1:68469/150‑1 (MQ=255)
                                                                                                                      cacTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGt                            <  2:854675/150‑1 (MQ=255)
                                                                                                                       acTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTa                           <  1:1787766/150‑1 (MQ=255)
                                                                                                                            tGTTAATCCATACAGCAACAGTACTGGGGTATCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgc                      <  1:1275259/150‑1 (MQ=255)
                                                                                                                             gTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgcc                     <  1:711607/150‑1 (MQ=255)
                                                                                                                              ttAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgccg                    <  2:1977472/150‑1 (MQ=255)
                                                                                                                                 aTCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGgccgccg                 >  2:261387/1‑150 (MQ=255)
                                                                                                                                     aacggacAACAGTACCAGGTTACCCCAGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCtt             >  1:196907/7‑150 (MQ=38)
                                                                                                                                      tACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTc            >  2:537534/1‑150 (MQ=255)
                                                                                                                                      tACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTc            <  2:1500582/150‑1 (MQ=255)
                                                                                                                                      tACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTc            >  1:1205021/1‑150 (MQ=255)
                                                                                                                                       aCAGCAACAGTACTGGGGTAACCTGTTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCt           <  1:1716100/150‑1 (MQ=255)
                                                                                                                                        ggacAACAGTACCAGGTTACCCCAGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTg          >  1:1662742/4‑150 (MQ=255)
                                                                                                                                        cAGCAACAGTACTTGGTTAACCTGGTAATGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAAACTAGAGAGCCTTTCTTGAGACAGGAATACATTGAAGACAAAGAACTAAAATTGACAAAGTTAAGTAGCGGCCGCCGCATTCTg          >  2:519494/1‑150 (MQ=255)
                                                                                                                                        cAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTg          >  1:1599795/1‑150 (MQ=255)
                                                                                                                                        cAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTg          >  1:2335914/1‑150 (MQ=255)
                                                                                                                                         aGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTgg         <  1:656625/150‑1 (MQ=255)
                                                                                                                                          gCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGa        <  1:1250477/150‑1 (MQ=255)
                                                                                                                                          gCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGa        >  2:1514777/1‑150 (MQ=255)
                                                                                                                                          gCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGACTGTAAACTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCGCATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGa        >  2:979572/1‑150 (MQ=255)
                                                                                                                                           cAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAg       >  1:2462622/1‑150 (MQ=255)
                                                                                                                                             aCAGTACTGGGGTAACCTGGTACTGTTGTCCGGTTTAGCATCCGGCACGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCTCCTTCTGGAGGc     <  1:406148/150‑1 (MQ=255)
                                                                                                                                                gTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGtt  <  2:1794560/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTT  >  NC_000913/933007‑933300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: