BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,408,537	A→T	7.2%	E163V (GAG→GTG)	zntB →	Zn(2(+)):H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,408,537	0	A	T	7.2%	351.3 / 12.8	119	E163V (GAG→GTG)	zntB	Zn(2(+)):H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (59/51); new base T (5/4); total (64/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 124 total aligned reads displayed.

GCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCC  >  NC_000913/1408388‑1408685

gCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGa                                                                                                                                                      <  2:864025/150‑1 (MQ=255)

  ggTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGagc                                                                                                                                                    >  1:2153520/1‑150 (MQ=255)

   gTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATCGCTGGTGGATGTCTCCGATGCGTTGACCGATCATTCCAGTGAATTTATCGagca                                                                                                                                                   >  2:2364375/1‑150 (MQ=255)

        ttGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGc                                                                                                                                              <  2:949461/150‑1 (MQ=255)

         tGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCa                                                                                                                                             >  1:1207308/1‑150 (MQ=255)

               gACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaa                                                                                                                                       >  2:2184413/1‑150 (MQ=255)

                aCCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATtaa                                                                                                                                      >  2:1478188/1‑150 (MQ=255)

                  ccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAAt                                                                                                                                    <  1:1400079/150‑1 (MQ=255)

                   cGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattc                                                                                                                                   <  2:2253022/150‑4 (MQ=255)

                   cGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAAtt                                                                                                                                   >  1:2436614/1‑150 (MQ=255)

                   cGACAACGCAAAGTGCTGCCGCTGGACGATGGGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattc                                                                                                                                   <  1:1077337/150‑4 (MQ=255)

                      cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTGCGACCGATTGCGGTGGATGGCTGGTGGATGTGTGCGATTCGTTGACGGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATc                                                                                                                                <  2:803098/150‑1 (MQ=255)

                          gCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAcc                                                                                                                            <  1:1175680/150‑1 (MQ=255)

                           cAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCt                                                                                                                           <  1:405056/150‑1 (MQ=255)

                             aaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTg                                                                                                                         <  1:1927532/150‑1 (MQ=255)

                                tGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAg                                                                                                                      <  1:2301788/150‑1 (MQ=255)

                                 gCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGa                                                                                                                     <  1:767311/150‑1 (MQ=255)

                                   tGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATa                                                                                                                   >  2:2464508/1‑150 (MQ=255)

                                    ggCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATaa                                                                                                                  >  2:1528164/1‑150 (MQ=255)

                                     gcgcTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAt                                                                                                                 <  1:414516/150‑1 (MQ=255)

                                     gcgcTGGACGAGGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAt                                                                                                                 <  2:2374861/150‑1 (MQ=255)

                                           gACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCtt                                                                                                           >  1:1059675/1‑150 (MQ=255)

                                              gATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGAt                                                                                                        <  1:172331/150‑1 (MQ=255)

                                               aTGTGGTGTGCGATCTGGTAGAGGGCACGGGTCCGACCGATTTCGGGGGATGGATGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATc                                                                                                       <  1:313955/150‑1 (MQ=255)

                                               aTGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATc                                                                                                       <  2:1166176/150‑1 (MQ=255)

                                                 gtggtgAGCGATCTGGAAGAGGGCACGGGTGCGACCGATTGCGGTGGATGGGTGGTGGATTTGTGCGATGCGTTGACCGATCATTCGAGTGAA‑TTATCGTGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATGTCCTTGTTCAGc                                                                                                    <  2:904925/150‑1 (MQ=255)

                                                   ggtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGct                                                                                                   <  2:1603031/150‑2 (MQ=255)

                                                   ggtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCa                                                                                                   >  1:1726259/1‑150 (MQ=255)

                                                   ggtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCa                                                                                                   <  1:90487/150‑1 (MQ=255)

                                                   ggtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCa                                                                                                   >  2:560276/1‑150 (MQ=255)

                                                      gAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAt                                                                                                >  1:721044/1‑150 (MQ=255)

                                                       aGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAtt                                                                                               <  1:195517/150‑1 (MQ=255)

                                                       aGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAtt                                                                                               <  2:1966564/150‑1 (MQ=255)

                                                          gATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCa                                                                                            >  2:343280/1‑150 (MQ=255)

                                                             cTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcg                                                                                         >  1:1738714/1‑150 (MQ=255)

                                                               ggAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcgcg                                                                                       >  1:671582/1‑150 (MQ=255)

                                                                  agagGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTgg                                                                                    <  2:721044/150‑1 (MQ=255)

                                                                        cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                              <  2:251123/150‑1 (MQ=255)

                                                                        cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                              >  1:1163330/1‑150 (MQ=255)

                                                                         aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTg                                                                             <  1:1104626/150‑1 (MQ=255)

                                                                          cGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTgg                                                                            >  1:1276701/1‑150 (MQ=255)

                                                                           gggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                           <  2:1059675/150‑1 (MQ=255)

                                                                           gggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                           <  2:953504/150‑1 (MQ=255)

                                                                             ggccgaccgaTTGCGGGGGATGGCTGGTGGATGTGCGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGctc                                                                         <  2:400099/148‑1 (MQ=255)

                                                                             gTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGctc                                                                         >  2:262768/1‑150 (MQ=255)

                                                                               ccgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctg                                                                       <  1:1005631/150‑1 (MQ=255)

                                                                                cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgc                                                                      <  2:170091/150‑1 (MQ=255)

                                                                                cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgc                                                                      >  1:2005746/1‑150 (MQ=255)

                                                                                cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgc                                                                      >  2:604948/1‑150 (MQ=255)

                                                                                  accgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgctg                                                                    >  2:2176137/1‑150 (MQ=255)

                                                                                   ccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCtgctgc                                                                   >  1:1290859/1‑150 (MQ=255)

                                                                                    cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTgcg                                                                  <  1:1528164/150‑1 (MQ=255)

                                                                                     gaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTgcgc                                                                 <  2:1590452/150‑1 (MQ=255)

                                                                                      aTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCa                                                                >  1:1311150/1‑150 (MQ=255)

                                                                                      aTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCa                                                                >  1:84835/1‑150 (MQ=255)

                                                                                      aTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCa                                                                <  1:2416607/150‑1 (MQ=255)

                                                                                      aTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCa                                                                <  2:643578/150‑1 (MQ=255)

                                                                                          cGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACa                                                            >  2:2072837/1‑150 (MQ=255)

                                                                                            gggtATCGCAGGTGGATGTGTGCGATGAGTAGACAGATCATTACAGTGAAATTATAGAGAAGCTGCAAGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaat                                                          >  2:2047705/1‑150 (MQ=255)

                                                                                            ggggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaat                                                          >  2:1630764/1‑150 (MQ=255)

                                                                                               gATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaa                                                       <  2:399853/150‑1 (MQ=255)

                                                                                                aTGGCTGGTGGATGTGTGCGATTCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCGCTGCTGCGCAAACaattaat                                                      <  2:1276701/150‑1 (MQ=255)

                                                                                                    cTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgtg                                                  >  2:1356384/1‑150 (MQ=255)

                                                                                                       gtggATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATg                                               <  2:1738714/150‑1 (MQ=255)

                                                                                                        tggATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGc                                              <  2:84835/150‑1 (MQ=255)

                                                                                                            tgtgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATTAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCg                                          <  1:1478188/150‑1 (MQ=255)

                                                                                                             gtgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGc                                         >  1:2040017/1‑150 (MQ=255)

                                                                                                              tgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCt                                        >  2:910029/1‑150 (MQ=255)

                                                                                                               gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCta                                       <  1:1918735/150‑1 (MQ=255)

                                                                                                               gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCta                                       >  2:1012274/1‑150 (MQ=255)

                                                                                                               gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCta                                       >  1:674797/1‑150 (MQ=255)

                                                                                                                tgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtat                                      >  1:515145/1‑150 (MQ=255)

                                                                                                                tgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtat                                      >  1:1539288/1‑150 (MQ=255)

                                                                                                                  cGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTatat                                    <  2:671582/150‑1 (MQ=255)

                                                                                                                       cGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCAc                               >  2:1081660/1‑150 (MQ=255)

                                                                                                                           gACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCa                           >  1:826834/1‑150 (MQ=255)

                                                                                                                           gACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCa                           >  1:1185719/1‑150 (MQ=255)

                                                                                                                             ccGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAAc                         >  1:127275/1‑150 (MQ=255)

                                                                                                                              ctaTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAATCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGGCGCCATATAGCGACGCatca                        <  1:2342881/148‑4 (MQ=255)

                                                                                                                               gATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAATATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAAATAATTGTGATGCGTCGCTATATGGcaacgcaacga                       >  2:1279625/1‑149 (MQ=255)

                                                                                                                               gATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                       >  1:1672057/1‑150 (MQ=255)

                                                                                                                                aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTg                      >  1:2450234/1‑150 (MQ=255)

                                                                                                                                 tCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGa                     <  2:1521073/150‑1 (MQ=255)

                                                                                                                                   aTTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATg                   <  1:481144/150‑1 (MQ=255)

                                                                                                                                   aTTCCAGTGAATTTATCGAGCAGCTGAACGATAAAATTATCGACCTTGAAGATAATCTACTAGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACAGCAACGTGATg                   >  2:184594/1‑150 (MQ=255)

                                                                                                                                       cAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTa               <  1:864143/150‑1 (MQ=255)

                                                                                                                                         ataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGTAACGTGATGTTTATg             >  2:2117174/6‑150 (MQ=255)

                                                                                                                                         ataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATg             >  1:2468488/6‑150 (MQ=255)

                                                                                                                                         ataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATg             >  1:1205358/6‑150 (MQ=255)

                                                                                                                                           gAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt           >  1:1424915/1‑150 (MQ=255)

                                                                                                                                           aattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt           >  1:1767498/4‑150 (MQ=255)

                                                                                                                                            aaTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTc          >  2:683165/1‑150 (MQ=255)

                                                                                                                                             aTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGAGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCg         >  1:1489565/1‑150 (MQ=255)

                                                                                                                                              tttATCGTGCAGCTGCTCGATAAAATTATCGCCCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGGTCGt        >  1:833470/1‑150 (MQ=255)

                                                                                                                                               ttATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGtg       >  1:2271082/1‑149 (MQ=255)

                                                                                                                                                tATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGTGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCt      >  1:573209/1‑150 (MQ=255)

                                                                                                                                                tATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCt      >  1:1667094/1‑150 (MQ=255)

                                                                                                                                                 aTCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCtt     >  1:643382/1‑150 (MQ=255)

                                                                                                                                                   cGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGc   >  1:2423873/1‑150 (MQ=255)

                                                                                                                                                    gAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGcc  >  2:826040/1‑150 (MQ=255)

GCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCC  >  NC_000913/1408388‑1408685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`