Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,444,038 C→A 5.2% L330M (CTG→ATG)  ydbH → PF11739 family protein YdbH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,444,0380CA 5.2% 1109.7 / 25.3 321L330M (CTG→ATG) ydbHPF11739 family protein YdbH
Reads supporting (aligned to +/- strand):  ref base C (149/154);  new base A (7/11);  total (156/165)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

GTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTGGAC  >  NC_000913/1443889‑1444187
                                                                                                                                                     |                                                                                                                                                     
gtgtCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCctc                                                                                                                                                       >  1:52034/1‑150 (MQ=255)
gtgtCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCctc                                                                                                                                                       >  2:2038585/1‑150 (MQ=255)
gtgtCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGAGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCctc                                                                                                                                                       >  2:249406/1‑150 (MQ=255)
 tgtCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCctct                                                                                                                                                      >  1:1589108/1‑150 (MQ=255)
   gcAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGc                                                                                                                                                    <  2:2122791/149‑1 (MQ=255)
    cAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCa                                                                                                                                                   >  2:2355912/1‑150 (MQ=255)
       aGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCt                                                                                                                                                <  1:376732/150‑1 (MQ=255)
       aGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCt                                                                                                                                                >  2:173483/1‑150 (MQ=255)
          cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGAc                                                                                                                                             <  1:2251428/150‑1 (MQ=255)
          cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGGCAGCGGACGACTGAGGGTGCGGACCCAGGGGCAAGCGGGCAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATACCAGTCAGCTGCAGAGGCAGCTGAc                                                                                                                                             <  2:2439656/150‑1 (MQ=255)
           gACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGAcc                                                                                                                                            >  2:2007020/1‑150 (MQ=255)
             cAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCgg                                                                                                                                          <  1:1057437/150‑1 (MQ=255)
             cAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCgg                                                                                                                                          <  1:1454017/150‑1 (MQ=255)
             cAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGGGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCgg                                                                                                                                          <  1:1839366/150‑1 (MQ=255)
              aaCTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCAGAGGCAGCTGActgtt                                                                                                                                         <  2:1931536/150‑5 (MQ=255)
               aCTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTg                                                                                                                                        <  2:1795270/150‑1 (MQ=255)
               aCTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTg                                                                                                                                        >  2:716198/1‑150 (MQ=255)
                  ggcaggcaGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAg                                                                                                                                     <  1:1823494/150‑1 (MQ=255)
                  ggcaggcaGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAg                                                                                                                                     >  1:2328766/1‑150 (MQ=255)
                   gcaggcagGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGc                                                                                                                                    <  1:463960/150‑1 (MQ=255)
                   gcaggcagGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGc                                                                                                                                    <  1:1089264/150‑1 (MQ=255)
                      ggcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                                                                                                                 <  1:1667757/150‑1 (MQ=255)
                      ggcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                                                                                                                 <  1:170595/150‑1 (MQ=255)
                      ggcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                                                                                                                 >  1:428183/1‑150 (MQ=255)
                      ggcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACACAGCGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCACAGGAAAATTAAGAATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                                                                                                                 >  2:1176902/1‑150 (MQ=255)
                        caggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAAc                                                                                                                               <  1:1338299/150‑1 (MQ=255)
                        caggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGCCAACGCGGTGCATAAATTTGGCCAAGGAAAATTAAGCAGGGATAACAGTCAGCTCCCTCTGCAGCTGACCGGTGAACCCAAAc                                                                                                                               >  2:2082946/1‑150 (MQ=255)
                            gCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGc                                                                                                                           >  1:2177636/1‑150 (MQ=255)
                             cTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCg                                                                                                                          >  2:1504423/1‑150 (MQ=255)
                               tGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGa                                                                                                                        >  1:742778/1‑150 (MQ=255)
                                tagaACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAATGGCAACGCGGTGCTTAATTTTTGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACGGGTGAAGCGAAACAGGCGGAc                                                                                                                       <  2:324664/149‑1 (MQ=255)
                                gagaACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                                                                                                                       <  1:1021957/150‑1 (MQ=255)
                                  gaACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCt                                                                                                                     <  1:798545/150‑1 (MQ=255)
                                  gaACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCt                                                                                                                     >  1:605740/1‑150 (MQ=255)
                                       ctctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCAttt                                                                                                                >  1:937041/1‑150 (MQ=255)
                                        tctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTTCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTGAGCTGCCTCTGCAGCTGAGCGGTGAAGCGAAACAGCCGGACCTCAtttt                                                                                                               <  2:2375723/150‑1 (MQ=255)
                                           ggTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTtata                                                                                                            >  2:348923/1‑150 (MQ=255)
                                                gCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGccc                                                                                                       >  1:2354304/1‑150 (MQ=255)
                                                 cGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCg                                                                                                      <  1:2038585/150‑1 (MQ=255)
                                                    acgacTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGttt                                                                                                   >  1:859467/1‑150 (MQ=255)
                                                     cgacTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTa                                                                                                  >  1:1886568/1‑150 (MQ=255)
                                                     cgacTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTa                                                                                                  >  1:1418894/1‑150 (MQ=255)
                                                       acTGAGTGTGCTGACCCAGGGGCAAGCTGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCTGACCTCATTTTATATGCCCGTTTAcc                                                                                                <  2:2199332/150‑1 (MQ=255)
                                                       acTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATATCAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTAcc                                                                                                <  2:1616500/150‑1 (MQ=255)
                                                       acTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTAcc                                                                                                >  2:1279022/1‑150 (MQ=255)
                                                          gAGTGTGCTGACACAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgc                                                                                             >  2:473456/1‑150 (MQ=255)
                                                            gtgtGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgcgc                                                                                           >  2:1866165/1‑150 (MQ=255)
                                                            cagagCTGACACAGGGGCAAGAGGGTAAGGGCAACGCGCTGCTTAATTTTGCCCCACCAAAATAAAGCATGCATAACAGTCAGCTGCCACTGCAGCTGACCGGTCAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgcgc                                                                                           >  2:1560829/5‑150 (MQ=255)
                                                             tgtgCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCa                                                                                          <  1:1465822/150‑1 (MQ=255)
                                                                gCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCt                                                                                       >  2:2143758/1‑150 (MQ=255)
                                                                gCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCt                                                                                       >  2:2240249/1‑150 (MQ=255)
                                                                 cTGCCCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCAGTTTACCTGCGCAGCTa                                                                                      >  1:1767532/1‑150 (MQ=255)
                                                                  tGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAGCGGGCGGGCCTCATGTTATATGCCCGTTTACGTGCGCAGCTag                                                                                     >  2:494156/1‑149 (MQ=255)
                                                                     cccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTg                                                                                  <  1:2286455/150‑1 (MQ=255)
                                                                     cccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTg                                                                                  >  2:559880/1‑150 (MQ=255)
                                                                      ccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTgg                                                                                 >  2:2290150/1‑150 (MQ=255)
                                                                      ccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTgg                                                                                 >  2:844/1‑150 (MQ=255)
                                                                          gggCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgt                                                                             <  2:919072/150‑1 (MQ=255)
                                                                          gggCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgt                                                                             >  1:2007386/1‑150 (MQ=255)
                                                                          gggCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGAATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgt                                                                             <  1:1209234/150‑1 (MQ=255)
                                                                             cAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCGGTTTACCTGCGCAGCTAAGTGGAAgtctg                                                                          <  1:491838/150‑1 (MQ=255)
                                                                              aaGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgtctgt                                                                         <  2:1589108/150‑1 (MQ=255)
                                                                               aGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgtctgtc                                                                        >  1:1146102/1‑150 (MQ=255)
                                                                               aGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgtctgtc                                                                        <  1:2190731/150‑1 (MQ=255)
                                                                               aGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCAGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGAGGAAgtctgtc                                                                        >  1:1391028/1‑150 (MQ=255)
                                                                                        gggCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAAc                                                               >  2:2402849/1‑150 (MQ=255)
                                                                                         ggCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACCGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACg                                                              >  1:2059432/1‑150 (MQ=255)
                                                                                          gCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGc                                                             >  2:676055/1‑150 (MQ=255)
                                                                                            aaCCCGGTGATTAATTTTGCCCCAGGAAAATTAAGAATGGATAACAGTCAGATGCCTATGAAGATGACCGGTGAAGCAAAAAAGGCGCACAACATTTTATATGCCCGTTTACATGCGAAGAAAATTGCAAGAATGACTGACCAAAccatg                                                           >  2:409645/1‑146 (MQ=255)
                                                                                               gcgGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGAcc                                                        <  1:716198/150‑1 (MQ=255)
                                                                                                 ggTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCtt                                                      <  2:2298630/150‑1 (MQ=255)
                                                                                                  gTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGAc                                                                    >  2:618247/1‑135 (MQ=255)
                                                                                                  gTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGAc                                                                    <  1:618247/135‑1 (MQ=255)
                                                                                                   tGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTg                                                    <  1:1238724/150‑1 (MQ=255)
                                                                                                    gCTTATTTTTGGCCCAGGAATATTTAGCATGGATAACCGTCAGGTGCCTCTGCAGCTGACCGGTGTAGCGAAACAGGCGGACCTCATTTTATATGCCCGTGTACCTGCTCAGCTAAGTGGAAGTCTTTCTTACCCAACGCTGACCTTTGa                                                   <  2:1711596/150‑1 (MQ=255)
                                                                                                    gCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGa                                                   >  2:1848973/1‑150 (MQ=255)
                                                                                                     cTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAg                                                  <  1:1279022/150‑1 (MQ=255)
                                                                                                         aTTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAg                                              >  1:2450822/1‑150 (MQ=255)
                                                                                                                cccAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGtt                                       <  1:822865/150‑1 (MQ=255)
                                                                                                                    ggAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACAGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACtt                                   <  2:2471224/150‑1 (MQ=255)
                                                                                                                     gAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTc                                  <  1:2482150/150‑1 (MQ=255)
                                                                                                                         aTTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTc                              <  2:742778/150‑1 (MQ=255)
                                                                                                                         aTTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGGCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGGTACTTCGTTc                              <  1:33334/150‑1 (MQ=255)
                                                                                                                              gCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGCCCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAgg                         >  1:2431876/1‑150 (MQ=255)
                                                                                                                               cATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAggg                        <  1:1919113/150‑1 (MQ=255)
                                                                                                                                  ggATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAag                     <  2:1418894/150‑1 (MQ=255)
                                                                                                                                    aTAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAagag                   >  2:1344030/1‑150 (MQ=255)
                                                                                                                                     tAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGt                  <  2:1205289/150‑1 (MQ=255)
                                                                                                                                     tAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGt                  >  2:784406/1‑150 (MQ=255)
                                                                                                                                      aaCAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTc                 >  2:635743/1‑150 (MQ=255)
                                                                                                                                        cggTCAGCTGCAGAGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCAt               >  2:86906/3‑150 (MQ=255)
                                                                                                                                         aGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATc              >  2:1818499/1‑150 (MQ=255)
                                                                                                                                          gTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCg             <  1:1800949/150‑1 (MQ=255)
                                                                                                                                             tgCTGCCTCTGCAGGTGAGCGGTGAAGCGAAACAGTCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGAtt          <  2:1767532/149‑1 (MQ=255)
                                                                                                                                             aGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGAtt          >  1:679982/1‑150 (MQ=255)
                                                                                                                                                tGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGc       >  1:1547079/1‑150 (MQ=255)
                                                                                                                                                tGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGc       >  1:352168/1‑150 (MQ=255)
                                                                                                                                                 gCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCt      <  1:2089328/150‑1 (MQ=255)
                                                                                                                                                  ccTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTg     >  1:991641/1‑150 (MQ=255)
                                                                                                                                                   ctctGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTgg    >  2:233217/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTGGAC  >  NC_000913/1443889‑1444187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑