BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,702,495	C→G	5.8%	A88G (GCC→GGC)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,702,495	0	C	G	5.8%	465.9 / 14.0	139	A88G (GCC→GGC)	add	adenosine deaminase
Reads supporting (aligned to +/- strand): ref base C (61/68); new base G (6/2); total (68/71)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

Only 100 of 141 total aligned reads displayed.

TCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCG  >  NC_000913/1702347‑1702644

tCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGcc                                                                                                                                                      <  1:532355/150‑1 (MQ=255)

  ccTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg                                                                                                                                                    <  1:460215/150‑1 (MQ=255)

  ccTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg                                                                                                                                                    >  1:617551/1‑150 (MQ=255)

       aaaCACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACg                                                                                                                                               >  1:869482/1‑150 (MQ=255)

         acacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGc                                                                                                                                             <  1:1148557/150‑1 (MQ=255)

         acacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGc                                                                                                                                             <  1:2063257/150‑1 (MQ=255)

          cacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGcc                                                                                                                                            >  1:841018/1‑150 (MQ=255)

              gATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGCCCACACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCAcggccggcc                                                                                                                                        >  2:1102789/1‑149 (MQ=255)

              gATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCa                                                                                                                                        >  2:358329/1‑150 (MQ=255)

               aTTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCAc                                                                                                                                       <  2:1275763/150‑1 (MQ=255)

                 tCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGGCCGTGACGGGCTGCatct                                                                                                                                     <  2:132377/150‑4 (MQ=255)

                 tCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTa                                                                                                                                     <  2:1469783/150‑1 (MQ=255)

                 tCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTa                                                                                                                                     <  1:2433062/150‑1 (MQ=255)

                  ccccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTAt                                                                                                                                    >  1:2004991/1‑150 (MQ=255)

                   cccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATg                                                                                                                                   >  1:1597083/1‑150 (MQ=255)

                    ccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGt                                                                                                                                  >  1:1520588/1‑150 (MQ=255)

                    ccACGTTCAGGTCATTGCCAACGAACCCGATCTGCTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGt                                                                                                                                  >  2:2083779/1‑150 (MQ=255)

                     cACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTc                                                                                                                                 >  1:581362/1‑150 (MQ=255)

                       cGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGa                                                                                                                               <  1:54017/150‑1 (MQ=255)

                         ttCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGc                                                                                                                             <  1:35515/150‑1 (MQ=255)

                           cAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTg                                                                                                                           <  2:628540/150‑1 (MQ=255)

                            aGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGc                                                                                                                          <  1:620975/150‑1 (MQ=255)

                               tCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGtt                                                                                                                       >  1:1234917/1‑150 (MQ=255)

                                cATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGttt                                                                                                                      >  2:1567678/1‑150 (MQ=255)

                                cATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGttt                                                                                                                      <  1:672749/150‑1 (MQ=255)

                                 aTTTCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGtttt                                                                                                                     <  2:2320896/150‑1 (MQ=255)

                                 aTTGCCAACGAACCCGATCTGGTGAGCTTTCTGCCCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGtttt                                                                                                                     <  2:1788729/150‑1 (MQ=255)

                                   tGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTTGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTc                                                                                                                   <  2:1870090/150‑1 (MQ=255)

                                       aaCGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGACCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCa                                                                                                               >  1:69756/1‑150 (MQ=255)

                                         cGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAgg                                                                                                             >  2:2311135/1‑150 (MQ=255)

                                           aaCCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCt                                                                                                           >  2:1440142/1‑150 (MQ=255)

                                           aaCCCGATCTGGTGAGCTTTATGAACACACTTTACTGGGGCGTTAAAGTTCTCGCCTCTATTGATGCCTGTCGCCGCGTTGCATTTGAAAACATTGAATATGCAGACCGGCAAGTCCTGCACTATGGCTAGCTTCTTTTTTCACCATGCt                                                                                                           <  2:2424566/150‑1 (MQ=255)

                                                gATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatg                                                                                                      >  2:1190586/1‑150 (MQ=255)

                                                 aTCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatgg                                                                                                     <  1:1435482/150‑1 (MQ=255)

                                                  tCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggc                                                                                                    <  2:2420701/150‑1 (MQ=255)

                                                  tCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggc                                                                                                    <  1:2222382/150‑1 (MQ=255)

                                                     ggTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaat                                                                                                 >  2:2123267/1‑150 (MQ=255)

                                                       tGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatgg                                                                                               <  1:2083779/150‑1 (MQ=255)

                                                        gAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggc                                                                                              >  1:1311432/1‑150 (MQ=255)

                                                        gAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggc                                                                                              <  2:1355561/150‑1 (MQ=255)

                                                              tCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCGGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCa                                                                                        <  2:1218870/150‑1 (MQ=255)

                                                                 gACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCATGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATCGCAATGGCACATCAGCt                                                                                     >  2:2193888/1‑150 (MQ=255)

                                                                  aCCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTg                                                                                    <  1:1181068/150‑1 (MQ=255)

                                                                   ccAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGc                                                                                   >  2:1814582/1‑150 (MQ=255)

                                                                    cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGcc                                                                                  <  1:1309656/150‑1 (MQ=255)

                                                                    cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCACTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGcc                                                                                  <  1:2340563/150‑1 (MQ=255)

                                                                     aaaCTTGACTGGGGCGGTAAAGTTCTCGCCTCTCTGGATGCCTGTCGCCGCGTTGCATTTGAAAACATTGAAGATGCAGCCCGTCACGCCCTGCACTATGTCGAGCTGCGGTTTTCACGAGGCTCCATGGCAATGGCCCATCAGCTGCCt                                                                                 >  2:2168844/1‑150 (MQ=255)

                                                                             cTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCggg                                                                         <  1:444839/150‑1 (MQ=255)

                                                                             cTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCggg                                                                         <  2:863311/150‑1 (MQ=255)

                                                                             cTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCggg                                                                         <  1:1648755/150‑1 (MQ=255)

                                                                             cTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCggg                                                                         >  1:1847631/1‑150 (MQ=255)

                                                                               ggggCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGgtg                                                                       <  1:2401146/150‑1 (MQ=255)

                                                                                gggCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGgtgt                                                                      <  1:2026149/150‑1 (MQ=255)

                                                                                  gCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGtgttg                                                                    >  1:1524302/1‑150 (MQ=255)

                                                                                  gCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGtgttg                                                                    >  1:1694386/1‑150 (MQ=255)

                                                                                   cGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGtgttgt                                                                   >  1:32278/1‑150 (MQ=255)

                                                                                      tAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGa                                                                >  2:561655/1‑150 (MQ=255)

                                                                                      tAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGa                                                                <  2:474272/150‑1 (MQ=255)

                                                                                      tAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGa                                                                >  1:1244196/1‑150 (MQ=255)

                                                                                      tAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGa                                                                >  1:1848157/1‑150 (MQ=255)

                                                                                           ttCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTTCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCgg                                                           <  2:837624/150‑1 (MQ=255)

                                                                                             ctcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTg                                                         >  2:2439356/1‑150 (MQ=255)

                                                                                               cGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGGATTATGTATAGCTGCGTTTTTCACGAGGCTACATGTCAATGTCACTTTTGCTGCATTTATCGGGTTTTGTCGTAGCGTTGAt                                                       <  2:841018/150‑1 (MQ=255)

                                                                                               cGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGAt                                                       >  1:253270/1‑150 (MQ=255)

                                                                                                gCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATc                                                      <  2:1220155/150‑1 (MQ=255)

                                                                                                    ctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATg                                                  <  2:1877805/150‑1 (MQ=255)

                                                                                                      ctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGc                                                >  1:1259013/1‑150 (MQ=255)

                                                                                                        tGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGt                                              <  2:1171560/150‑1 (MQ=255)

                                                                                                                gTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAg                                      >  1:1312926/1‑150 (MQ=255)

                                                                                                                gTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAg                                      >  1:142599/1‑150 (MQ=255)

                                                                                                                  cgccgcGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGt                                    >  2:448055/1‑150 (MQ=255)

                                                                                                                    ccgcGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTg                                  >  2:709845/1‑150 (MQ=255)

                                                                                                                      gcgTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGcc                                <  1:2442616/150‑1 (MQ=255)

                                                                                                                      gcgTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGcc                                <  2:879792/150‑1 (MQ=255)

                                                                                                                       cgTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCg                               <  2:6085/150‑1 (MQ=255)

                                                                                                                        gTGGCATTTGAAAACATTGAAGATGCGGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGc                              <  1:1668740/150‑1 (MQ=255)

                                                                                                                          ggCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTACCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCAc                            >  1:834977/1‑150 (MQ=255)

                                                                                                                             aTTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCCAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGCTTGCCGCACCtt                         >  2:867702/1‑150 (MQ=255)

                                                                                                                              tttGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCttt                        <  1:2484727/150‑1 (MQ=255)

                                                                                                                                tGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTgg                      <  2:69756/150‑1 (MQ=255)

                                                                                                                                tGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTgg                      <  2:253270/150‑1 (MQ=255)

                                                                                                                                  aaaaCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGgtg                    >  1:1646641/1‑150 (MQ=255)

                                                                                                                                  aaaaCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGgtg                    >  1:1601998/1‑150 (MQ=255)

                                                                                                                                   aaaCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGgtgt                   <  2:1478411/150‑1 (MQ=255)

                                                                                                                                     aCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGc                 >  2:1689177/1‑150 (MQ=255)

                                                                                                                                      cATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCa                <  2:159028/150‑1 (MQ=255)

                                                                                                                                        gacatagtGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAgg              >  1:29613/8‑150 (MQ=255)

                                                                                                                                             gATGCAGCCCGTCACGGCCTGCTCTATGTCGAGCTACGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAg         <  1:2460409/150‑1 (MQ=255)

                                                                                                                                             gATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAg         >  2:620652/1‑150 (MQ=255)

                                                                                                                                             gATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAg         <  1:620652/150‑1 (MQ=255)

                                                                                                                                             agtGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAg         >  2:1254981/3‑150 (MQ=255)

                                                                                                                                              gtGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc        >  2:881382/2‑150 (MQ=255)

                                                                                                                                              gtGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc        >  2:788339/2‑150 (MQ=255)

                                                                                                                                              aTGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc        <  1:2013223/150‑1 (MQ=255)

                                                                                                                                              aTGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc        <  2:581362/150‑1 (MQ=255)

                                                                                                                                              aTGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCCTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc        >  1:1479534/1‑150 (MQ=255)

                                                                                                                                               tGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCt       >  1:1753119/1‑150 (MQ=255)

                                                                                                                                               tGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCt       >  2:1520285/1‑150 (MQ=255)

                                                                                                                                               tGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCt       <  2:1520588/150‑1 (MQ=255)

                                                                                                                                                    cccGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCg  <  1:561655/150‑1 (MQ=255)

TCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCG  >  NC_000913/1702347‑1702644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`