| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,758 | G→C | 20.0% | E614Q (GAA→CAA) | rsxC → | SoxR [2Fe‑2S] reducing system protein RsxC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,758 | 0 | G | C | 20.0% | 17.1 / 3.8 | 20 | E614Q (GAA→CAA) | rsxC | SoxR [2Fe‑2S] reducing system protein RsxC |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base C (2/2); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGA > NC_000913/1708612‑1708901 | gcgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAc < 2:1961341/150‑1 (MQ=255) tGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACAAAAGAAGCACCACCAAAAGGCTAATACGGAAAAAGAATAACAGGAAGATACGAGCAAAg > 2:2282650/1‑150 (MQ=11) cctaCAGCCTAATGCGGAACCAGAACACCAGGGGGATCCCCGGAAAGCCGCCGTCGAAGCCGCTATGGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGt < 1:881336/147‑1 (MQ=14) tAATTCGGAACCCGAAGAACACGGCGATCCTCGCAAAGCTGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGTAACCAGAAGAACATGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc < 2:1959361/150‑1 (MQ=11) tAATTCGGAACCAGAACCACAGGTCGATCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc < 2:1964450/150‑1 (MQ=255) cagCTATCGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt < 2:1061427/72‑1 (MQ=9) catcgCCCGTGCCAATGCTCGCAAGCTTTAACAGCAACAGGCTAATGCGGAACCAGAATAACAGGTCGATCCGCGCAAAGCCGCCTTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGcc < 2:16548/146‑1 (MQ=11) tGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAGCAGCGACCGGCGAATGCCGAGCCAgc > 1:1228620/1‑149 (MQ=11) gCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGc > 1:1234801/1‑92 (MQ=255) cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATCCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCAGAGCCAGAACAagagat > 2:929727/1‑148 (MQ=255) cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTAGCAGCGGCTATTGCCCGAGCGCACGCCCGCAACCAGGACCAGCAGCAGGCTAAAGACGAGCCCGAACACGAGGTCgcaacccc > 1:1152138/1‑143 (MQ=2) gCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAg < 1:646945/97‑1 (MQ=37) aaCAGGCTAATCCGGAACCAGAACAACAGGCCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCg < 1:1918976/150‑1 (MQ=11) ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAg < 2:1929638/74‑1 (MQ=34) aTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:264466/1‑111 (MQ=37) aTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 2:264466/111‑1 (MQ=37) cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 2:2426784/105‑1 (MQ=255) cGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg > 1:2426784/1‑105 (MQ=25) ggAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGACCAGCAACCGGCTAATGCCGAGCCAGAACCACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGc > 1:1205165/1‑150 (MQ=14) ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCAGCTATTGACCGAGACAAAGAACGAAAGCTGGAAAAGAAAAAGGCTAATGCCGAGCCAGAAGAACAGGTAGATACGCGCAAAGCGGCCGTCGAAGCCGCTAGTGCCCgc > 2:928119/1‑149 (MQ=11) | GCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGA > NC_000913/1708612‑1708901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |