Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,637,6451.T100.0% 172.6 / NA 56L2L (CTA→TTA) pitAmetal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (31/25);  total (31/25)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCG  >  NC_000913/3637509‑3637782
                                                                                                                                         |                                                                                                                                         
tataAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑ttacat                                                                                                                                     <  1:996058/143‑6 (MQ=255)
     gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAtttgtt                                                                                                                                 >  2:551133/1‑143 (MQ=255)
     gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAAGGCGCCGCGTTCATGTCCTCAACATGGCGTAACGTCCTGTGCTTACAtttgtt                                                                                                                                 >  1:90256/1‑143 (MQ=255)
       gattgcatttttGAGTCAAACCCATACAGGGGGCAAATCAAAAAAACTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAtttgtttg                                                                                                                               <  2:54829/136‑1 (MQ=255)
        aaTTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGc                                                                                                                              <  2:11975/143‑1 (MQ=255)
          ttattTTTTGAGTGAAATCTTTACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCCCGAAAGTCACTGATAATGCGTCGCGTTCATGTCCTCCAAATGGCGTAACGTCCTATGCTTACATTTCTTCGCtt                                                                                                                            >  2:942999/1‑142 (MQ=255)
            attTTTTGAGTGAAATCCATACAGGGGGCAAAGCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGc                                                                                                                          <  1:136185/143‑1 (MQ=255)
             ttttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTCCATTTGTTTGCTGGcc                                                                                                                         >  2:510895/1‑143 (MQ=255)
               ttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGTTGGCCTg                                                                                                                       >  2:992621/1‑143 (MQ=255)
                 tggAGTGAAATCCACACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGa                                                                                                                     <  1:901889/141‑1 (MQ=255)
                    aGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGAttt                                                                                                                  <  2:99709/143‑1 (MQ=255)
                    aGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGAttt                                                                                                                  >  1:420459/1‑143 (MQ=255)
                      tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGc                                                                                                                <  1:539884/143‑1 (MQ=255)
                           tCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAACGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATAca                                                                                                           >  1:562636/1‑142 (MQ=255)
                              aTACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACGTTTGTTTGCTGCCCTGGATTTGCATACCggg                                                                                                        >  2:12526/1‑143 (MQ=255)
                                 cAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTg                                                                                                     <  2:416479/143‑1 (MQ=255)
                                 cAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTg                                                                                                     <  1:802293/143‑1 (MQ=255)
                                       gCAAATCAAATAAATTCTATATGTCACTTTGCCCGCTCCTCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCGAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatta                                                                                               <  1:709608/143‑1 (MQ=255)
                                       acgaagcAAACAACGGCTCTATTCCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAACATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatta                                                                                               <  2:295529/137‑1 (MQ=255)
                                         aaaTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTattatt                                                                                             >  1:101850/1‑143 (MQ=255)
                                           agcAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgc                                                                                           <  2:588436/141‑1 (MQ=255)
                                            tCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgct                                                                                          >  1:985649/1‑143 (MQ=255)
                                              aaaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttg                                                                                        <  1:838201/143‑1 (MQ=255)
                                               aaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGTCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttgc                                                                                       <  2:695015/143‑1 (MQ=255)
                                                aaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCa                                                                                      >  1:187976/1‑143 (MQ=255)
                                                  aaaGGCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACt                                                                                    <  1:105578/143‑1 (MQ=255)
                                                     gtttataTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGc                                                                                 <  2:394715/140‑1 (MQ=255)
                                                         atatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt                                                                             <  1:509769/143‑1 (MQ=255)
                                                          tatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTGGTTTGCTGGCGTGGCTTGGCATACCGGGCTGTTATTATTGCTTGCGCTGGGTTTtg                                                                            >  1:537143/1‑143 (MQ=255)
                                                                  tttGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTc                                                                    >  1:507572/1‑143 (MQ=255)
                                                                   ttGCCCGCGCCGCGAAAGTCCCTGATAATGCGACGCGTTCATGTCCTCTGACTGGCGTAGCGT‑ATATGCTTACATTTGTTTGCTGGCCTGGATTTGCATCCCGGGCGGATTGTGTTGCTTGCACTGGCTTTTGTGCTGTTCTa                                                                  <  2:770782/143‑1 (MQ=255)
                                                                   ttGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCt                                                                   >  2:924160/1‑143 (MQ=255)
                                                                       ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGa                                                               >  2:338708/1‑143 (MQ=255)
                                                                        cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa                                                              <  1:791264/143‑1 (MQ=255)
                                                                             cgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAt                                                         <  1:917852/143‑1 (MQ=255)
                                                                               cgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACCAAGCCATCa                                                       >  1:996054/1‑143 (MQ=255)
                                                                                           aTAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCCATGGTTTCCacg                                           >  2:363898/1‑141 (MQ=255)
                                                                                             aaTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACAGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACTTAGCCATCAATGGTTTCCATgtc                                         >  2:610118/1‑141 (MQ=255)
                                                                                                  gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTGCCATGACACAGc                                    >  1:263351/1‑143 (MQ=255)
                                                                                                  gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGAATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTGTACGAAGCCATCAGTGGTTGGAATGAcacaat                                    >  1:895815/1‑141 (MQ=255)
                                                                                                     gcgTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTGGTGTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAGCAATGGTTTCCATGACACAGCCaa                                 <  2:748858/143‑1 (MQ=255)
                                                                                                          cATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTCCGGAGCCATCAATGATTTGCGTGCTTCAGCCAActctg                            >  2:656899/1‑139 (MQ=255)
                                                                                                              tCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAGCGCCGTgac                        >  1:531263/1‑141 (MQ=255)
                                                                                                               ccTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCa                       <  1:924160/143‑1 (MQ=255)
                                                                                                                cTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGcga                      >  2:695760/1‑141 (MQ=255)
                                                                                                                  cAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAGcc                    >  2:976805/1‑143 (MQ=255)
                                                                                                                  cAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAAcc                    >  1:674707/1‑143 (MQ=255)
                                                                                                                   aaaaTGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTgg                         <  2:706147/137‑1 (MQ=255)
                                                                                                                   aaaaTGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTgg                         >  1:706147/1‑137 (MQ=255)
                                                                                                                         gCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt             <  1:942999/143‑1 (MQ=255)
                                                                                                                           gTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACGCACCCAACGCCGTGGCAACCGTTACCtat           >  1:729360/1‑143 (MQ=255)
                                                                                                                             aaCGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAc         <  1:551133/143‑1 (MQ=255)
                                                                                                                               cGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTGTACGAAGCCATCAATGGTTTCCATGTCACAGCCAACGCCTTGGCCACCGTTCTCTCTAccc       >  2:753505/1‑143 (MQ=255)
                                                                                                                               cGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAccc       >  1:807789/1‑143 (MQ=255)
                                                                                                                                gTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCcg      <  2:420459/143‑1 (MQ=255)
                                                                                                                                   cTATGCTTACATTTGATTCATAGTTTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATCACACAGCCAACGCCGTGGCAACCGTTATCTATACCcgcgc   >  2:889807/1‑143 (MQ=255)
                                                                                                                                    tATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGAGTGCACTGGGTTTTGTGAAGTTCTACGAAGCCATCAATGGTTTCCATGGCACAGCCAACGCCGTGGCAACCGTTATCTATACCcccgag  >  1:854070/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCG  >  NC_000913/3637509‑3637782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑