New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3261397 =	46 (0.890)	3 (0.060)	3/254	NT	6.4%	coding (1022/2295 nt)	tdcE	2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
	?	NC_000913	3261423 =	43 (0.860)					coding (996/2295 nt)	tdcE	2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4

AAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGTAGATATCGAGGAACGAT  >  NC_000913/3261281‑3261564                                                                                                                                               |                                                                                                                                              aaaGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGgag                                                                                                                                               <  1:882324/143‑1 (MQ=255) aaaGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTGTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGCTCGGGTCGCAGgag                                                                                                                                               >  2:158512/1‑143 (MQ=255)      agtgTCAGGTTAGGTTCCGGTGGCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCGCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAg                                                                                                                                          <  1:132839/140‑1 (MQ=255)              ggtaggttCCGGTGCCGGCCCGATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCaa                                                                                                                                  <  2:1006239/141‑1 (MQ=255)                    ttCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCg                                                                                                                            <  1:613664/143‑1 (MQ=255)                     tCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCgg                                                                                                                           <  1:712733/143‑1 (MQ=255)                     tCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCgg                                                                                                                           >  2:193129/1‑143 (MQ=255)                            ccGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGc                                                                                                                    <  2:559354/143‑1 (MQ=255)                             cGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTGTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCa                                                                                                                   <  1:336347/143‑1 (MQ=255)                                            cAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGACTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATAc                                                                                                    >  1:970767/1‑143 (MQ=255)                                                gtgtGCAAAGCGCGGGAGGAGTTTCACGTCTCCGGCGTCCGGCGGTCCAGCCCCGTCCCGCCGATCACTCCCGTCGCCTAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGAt                                                                                                <  2:875144/143‑1 (MQ=255)                                                 tgtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCGATCCCGCCGATCACTTCCGTCGCCGAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGATGTACGCAGGGAGCGTACCATACGCATc                                                                                               >  2:609034/1‑143 (MQ=255)                                                      aaaTAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCat                                                                                          >  2:266010/1‑143 (MQ=255)                                                             ggAAGGAGTTTTTGGTCACCAGGGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGGGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAg                                                                                   >  1:273199/1‑143 (MQ=255)                                                                 ggAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgat                                                                               <  1:253154/143‑1 (MQ=255)                                                                  gAGTCTTTGTTCACTAGCGTACGCCCGTCCAGCCCCATCCCGCCTATCACTTGCGTCGCCCAGATTGGGTCGCTGGAGAACTGCGGATGAAATTCCGGTGTACGCAGGAAGCGTACCATTCTGTTCTTCATGATGAAGTgatc                                                                              <  2:55364/143‑1 (MQ=255)                                                                     tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgat                                                                           >  1:198258/1‑143 (MQ=255)                                                                         tGGTCACCAGCGTACGACGGTCCAGGGGTGTGCCGGCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGt                                                                       <  1:787015/143‑1 (MQ=255)                                                                               ccAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGGACAGCGAATCAAATTCCGGTGTACGCAGGACGCGTCCCATCCGGATCTTCATGATGAAGTGATCGATCAGTTCCtgt                                                                 >  2:27582/1‑143 (MQ=255)                                                                               ccAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCATCATGATGAAGTGCTCCACCAGTTCCtgt                                                                 >  2:935/1‑143 (MQ=255)                                                                                 ggCGTACGGCCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGc                                                               <  2:1013147/142‑1 (MQ=255)                                                                                  gCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCACATTCCGGTGTACGCAGGAAGCGTACCATGCGCATCTTCATGATGAAGTGATCGATCAGTTCCTGTGcc                                                              >  1:617199/1‑143 (MQ=255)                                                                                  gCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCCGTGcc                                                              >  1:368410/1‑143 (MQ=255)                                                                                   cGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGCTGAAGTGATCGCACAGTTCCCGTGCct                                                             >  1:683252/1‑143 (MQ=255)                                                                                    gTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGCAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctg                                                            <  1:622982/143‑1 (MQ=255)                                                                                       cGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCTCCCGGATGGGGTCGCCGGAGAACAGCGAATCAAATTCAGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgctg                                                         <  2:169228/143‑1 (MQ=255)                                                                                        gacctcccagccccagccCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCtgctgc                                                        <  1:667898/137‑1 (MQ=255)                                                                                        gACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCtgctgc                                                        >  2:105141/1‑143 (MQ=255)                                                                                        gACCGCCCAGCCCCATCCCGCCGAGCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCtgctgc                                                        <  2:116588/143‑1 (MQ=255)                                                                                           cGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTCCGCAGGAAGCGTACCATACGGATCTTCAGGCTGAAGTGACCGATCAGCTCCTGTGCCtgctgcccg                                                     >  1:859234/1‑140 (MQ=255)                                                                                             tCCAGCCCCAGCCGGCCGATCACTTCCGTCGCCCAGATTGAGACGCCGCTGTCTAGCGAGTCGGTTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCAtt                                                   <  2:450024/143‑1 (MQ=255)                                                                                               cAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGCTCAGTTCCTGTGCCGGCTGCTCGTTGa                                                 >  2:192499/1‑143 (MQ=255)                                                                                                gggcccATCCGGCCGAGCACGTCCGGCGGCCCGGGTGGGTTGCGGGAGAACTGCGAATCAAATGCCGGTGGACGCAGGAAGCGTACCATACGGATCTTCCTGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAg                                                <  2:327145/140‑1 (MQ=255)                                                                                                aGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGACGCGTACCATACGGATCTTCACGACGAAGCGATCGATCAGTTCCTGTGCCGGCTGCTCACTGAg                                                >  2:514010/1‑143 (MQ=255)                                                                                                         ccgccgATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGc                                       >  1:495636/1‑143 (MQ=255)                                                                                                         ccgccgATCACTTCCGTCGCCCAGATTGGGCAGCAAGGGGATATCTAATCAAATTCCGGTGTACGCAGGAAGCGTACCACACGGAGCTACATGAAGAAGTGATCGATCAGTACCTGGGCCGGCTGCTCATTGAGTACGCCAGc                                       >  2:885067/1‑143 (MQ=255)                                                                                                            ccgATTACTTCCGTCGCCCAGATTGTGTCGCCGGAGAACAGCGACTCAAATTCCGGTGTACGCAGGAAGCGTACTATACGGATCTTCATCATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCttt                                    <  2:335227/143‑1 (MQ=255)                                                                                                              gATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTaa                                  <  2:117390/143‑1 (MQ=255)                                                                                                                     tccggcgacccaatctgggcgacggaGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTc                                                                     >  2:534706‑M2/27‑101 (MQ=255)                                                                                                                     tccggcgacccaatctgggcgacggaGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTc                                                                     <  1:534706‑M2/75‑1 (MQ=255)                                                                                                                      ccggcgacccaatctgggcgacggaGAACAGCGAATCAAATTCCGGTGTACGCAGGCAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCCGCTCATTGAGTACGCCAGCTTTAAAGTCgcgc                          >  2:479476‑M2/26‑143 (MQ=255)                                                                                                                       cgccGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCt                         <  1:654394/140‑1 (MQ=255)                                                                                                                       CGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGCCATTCAGGATGAGGTGATAAACCAGGCCTTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCt                         <  1:792023‑M1/143‑1 (MQ=255)                                                                                                                       CGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCt                         <  2:777703‑M1/143‑1 (MQ=255)                                                                                                                             gcAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGGAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGt                   <  2:907570/142‑1 (MQ=255)                                                                                                                             ccAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGt                   >  1:1010770/1‑143 (MQ=255)                                                                                                                             ccAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCACATTGAGTACGCCAGCTTTCAAGTCGAGCTCAATgc                   >  2:654464/1‑142 (MQ=255)                                                                                                                             ccAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCCTCTCATTGAGTACGCCAGCTTTAATGTCGCGCTCGATGt                   >  1:546214/1‑143 (MQ=255)                                                                                                                              cAGAGTGAGTCGCCGGAGAACAGCGAATCAAATTCCGGGGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGTa                  <  1:169965/143‑1 (MQ=255)                                                                                                                                 aTTGGGTCGCCGCAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGTAGat               <  2:706798/143‑1 (MQ=255)                                                                                                                                              aGAACAGCGAAGCAAATGCCGGTGGACGCAGGAAGCGTACCATACGGATCTTCATGAGGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGTAGATATCGAGGAACGAt  <  2:1009975‑M2/142‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              AAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCGCTCAATGTAGATATCGAGGAACGAT  >  NC_000913/3261281‑3261564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.