Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	100%		insH21	insH21

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	57 [0]	[0] 57	insH21	insH21

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	51 (1.020)	41/254	NT	100%	intergenic (+253/‑33)	ychE/insH21	putative inner membrane protein/IS5 transposase and trans‑activator
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+151/‑484)	insH21/oppA	IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein

TACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299360‑1299640                                                                                                                                           |                                                                                                                                               tACAGGTAGTTATAAGGATTTTCTTAACTCCTTCTCCTCACTATAGTGTTCTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                            <  2:304880‑M1/143‑5 (MQ=255)  aCAGTTGGTTATAAGGATTTCCTTAACTGCTTCTCGTCATCATCATGTTATTTTCGCCACATCATAATCCTTGGCTTGCTGAAGAAGAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                                                                                                                           <  2:546451‑M1/143‑6 (MQ=255)   cAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                          <  1:607424‑M1/143‑7 (MQ=255)      ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTCCCTTTGGTAGAGGAAAGTTCTAAataataatc                                                                                                                                       >  1:48284‑M1/1‑134 (MQ=255)        agttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTCTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                     <  1:146706‑M1/143‑12 (MQ=255)          ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  2:740961‑M1/143‑14 (MQ=255)             taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATCATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                >  2:308432‑M1/1‑127 (MQ=255)             taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                >  2:535244‑M1/1‑127 (MQ=255)             taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                <  1:680073‑M1/143‑17 (MQ=255)             taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTCCTAAataataatcaattgtt                                                                                                                                >  2:98255‑M1/1‑127 (MQ=255)                    ttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGACTTGCTGCAGAATAATTGAAATGATATTATTAATGCCACTGCCTTTGATACAGCAAACTACTAAataataatcaattattaaattat                                                                                                                         >  2:685475‑M1/1‑120 (MQ=255)                        ttAACTGCTTCTCCTCTCCTTCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                     <  2:29223‑M1/143‑28 (MQ=255)                        ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttcaattattgtg                                                                                                                     >  2:114771‑M1/1‑116 (MQ=255)                        ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaacttattgtg                                                                                                                     >  1:92687‑M1/1‑116 (MQ=255)                        ctaACTGCTTCTCCTCACCTTCATGTTATTTTCGTCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                     <  2:290034‑M1/142‑28 (MQ=255)                           aCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAACGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                                                                                                                  <  1:710115‑M1/143‑31 (MQ=255)                             tggttctcCTCACCTTCATGGTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATGATGGAGATGATATTATTCATACCACTGCCTTTGGGAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                <  2:494533‑M1/140‑33 (MQ=255)                             tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                <  1:300277‑M1/143‑33 (MQ=255)                               cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                              <  2:630933‑M1/143‑35 (MQ=255)                                     ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                        <  1:416236‑M1/143‑41 (MQ=255)                                          catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                   >  1:205712‑M1/1‑98 (MQ=255)                                           atcatGTTATTTTCGCCACATCATAATCCGGGGCCTGGTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                                  <  2:448023‑M1/143‑47 (MQ=255)                                             catGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgta                                                                                                <  1:882331‑M1/143‑49 (MQ=255)                                              atGTGATTTTCGCCACATCATAAGCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaa                                                                                               <  1:839764‑M1/143‑50 (MQ=255)                                              atGCTACTTTCGCCACATCAGAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaa                                                                                               <  1:127035‑M1/143‑50 (MQ=255)                                               tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  1:833534‑M1/143‑51 (MQ=255)                                               tGTTATTTTCGCCACATCATAATCCGGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                              <  1:444045‑M1/143‑51 (MQ=255)                                                      tacgcCACAGCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                       <  1:647747‑M1/141‑58 (MQ=255)                                                        cGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaga                                                                                     <  2:188557‑M1/143‑60 (MQ=255)                                                          ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGACAGTGCTAAataataatcaattgttaaattattgtgcatttcactcctggaactgtaatcagaaaagata                                                                                   >  1:395626‑M1/1‑82 (MQ=255)                                                                 atAATCCTGGGCTTGCTGAAGAATAATTGAAAAGATATGAGTTATTCCCCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                            >  1:194367‑M1/1‑75 (MQ=255)                                                                     tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                        >  2:315564‑M1/1‑71 (MQ=255)                                                                        tGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagagagacatgcttagcca                                                                     >  1:76478‑M1/1‑68 (MQ=255)                                                                           gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                  <  1:400232‑M1/143‑79 (MQ=255)                                                                           gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                  <  2:558527‑M1/143‑79 (MQ=255)                                                                            cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatct                                                                 >  2:193425‑M1/1‑64 (MQ=255)                                                                            cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatct                                                                 >  2:455889‑M1/1‑64 (MQ=255)                                                                             ttGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcccgtctc                                                                >  1:370700‑M1/1‑63 (MQ=255)                                                                               gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctcta                                                              >  2:257843‑M1/1‑61 (MQ=255)                                                                                 tGAAGAATAATTGAAATGATATTATTAATGCCACTGCCTTGGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                            <  1:288216‑M1/143‑85 (MQ=255)                                                                                   aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttg                                                          <  2:865883‑M1/143‑87 (MQ=255)                                                                                      aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                       <  2:867464‑M1/143‑90 (MQ=255)                                                                                        taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattga                                                     <  2:915941‑M1/143‑92 (MQ=255)                                                                                           gagaaAAGAGATTGGTAATACCACGGCCTGTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                  <  1:29120‑M1/141‑95 (MQ=255)                                                                                               aaTTATATTAGTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactacttgaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                              <  2:504818‑M1/143‑99 (MQ=255)                                                                                                 tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatggaaagc                                            >  1:675895‑M1/1‑43 (MQ=255)                                                                                                       tattaATTCCACTGCCTTTGGTAGAGAAAAGTGCTAAataataatcaattgttaaattatggtgcatatcactactgaaactgtaatcaaaaaagatagacatacttagccaataacaatttgattaaattgaaagatgttcg                                      >  2:329567‑M1/1‑37 (MQ=255)                                                                                                           aaTTCCACTGCGTTTGATAGAGGAAAGTTCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                  <  2:192172‑M1/143‑111 (MQ=255)                                                                                                               ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgacagatgtttgttaaggca                              >  1:483427‑M1/1‑29 (MQ=255)                                                                                                                   tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgctaaggcaggga                          >  2:923194‑M1/1‑25 (MQ=255)                                                                                                                      cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgc                       >  2:955281‑M1/1‑22 (MQ=255)                                                                                                                                 gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat            >  2:257507‑M1/1‑11 (MQ=255)                                                                                                                                 gAAAGTGCTAAataataatcaattgttaaattattgtgcatctcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat            >  2:702964‑M1/1‑11 (MQ=255)                                                                                                                                  aatgtgcTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatt           <  1:131947‑M1/140‑134 (MQ=255)                                                                                                                                     gTGCTAAataagaatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctg        <  1:440429‑M1/143‑137 (MQ=255)                                                                                                                                       gCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat      <  2:194367‑M1/143‑139 (MQ=255)                                                                                                                                           aataataatcaattgttaatttattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaacgatgtttgttaaggcatggatgcaagctatagattctgatacgg  >  1:335853‑M1/1‑1 (MQ=255)                                                                                                                                           |                                                                                                                                               TACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299360‑1299640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑