| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 100.0% | 107.6 / ‑6.7 | 42 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (20/19); minor base G (1/1); total (22/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA > NC_000913/125247‑125523 | agctCCTCGCGAAAGCCTACGGCGCAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCTTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAg < 1:750520/140‑1 (MQ=255) tCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGACACTCCGCGCGTTCCGTATATCGCTTAGGtg > 1:711766/1‑143 (MQ=255) ccggCGAAAGATGACGGCGTAGGTTCTGACGTTTATAGAGTGACCTCCAACAAAGAAAAGGAGAGTGAACGCCAGAACTGTGCACGCTGGAACATACAAAAACAACAAAAAAATATGCGCGTTCCGTATATCGCTTAGGtgat < 2:942382/141‑1 (MQ=255) tGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCGTCACCGAGCTGGCGCGTGATGGTGAGGATTGTGAACGCTGGAACATGCTGGACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGggatg > 1:442038/1‑143 (MQ=255) gCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCGCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACCTGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATGTCGCTTAGGTGATGaa > 1:142100/1‑143 (MQ=255) tgAAAGATTCCGGCGGGGTTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCCCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAac < 2:643232/142‑1 (MQ=255) aaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATGGCGTAGGTGATGAacgac > 1:283152/1‑143 (MQ=255) tACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGCTGAACGACGCTcc > 2:307667/1‑143 (MQ=255) ttCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCAt < 1:863348/143‑1 (MQ=255) tggcGTTGATAGCGGGACCGCCGTCTCCGAGCTGGCGCGTGGTGGTCAGGATTGTGAACGCGGGAACATGCTGCACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTa < 1:388095/140‑1 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTCATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTCGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAcc > 2:434696/1‑143 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTACGGATTGTGAACGCTGGAAAATGCTGCAGCCGCTGGACACTCCGCGCGTTCCGCATATCGCTCAGGTGATTAACGACGCCCCCGGAGTCGCGACTACCgc > 2:181719/1‑142 (MQ=255) taGCGTGACCTCCTTCACCGAGCTCGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtata < 1:272921/143‑1 (MQ=255) cGTGCCCGCCTTCGCCGGGCTGGCGCGTCATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 2:142100/143‑1 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATAGCGCATAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGGCTAGATGAGACTGtt > 2:315443/1‑143 (MQ=255) cctTCACCGAGCGGGCGCGTGATAGTACGGAGTGAAAACTCTTGAACATGCTGCCCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc < 1:185258/143‑1 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCGGTATATCGCTTAGGTGATGAACGACGCTCCGGGAGTGGCATCTACCGACTCTATGAAACTGTTCg > 1:312611/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg > 1:80626/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGGTCCGTATATCGCCTAGGTGATGAACGCCGCTACGGCAGTGGCAGCTACCGACGGTATGAAACTGCTCg > 2:421627/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGGCAGGAGTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 1:682791/143‑1 (MQ=255) aCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGGGGCATCTACCGACTGTATGAAACTGTTGGCTGa > 1:106123/1‑143 (MQ=255) cGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGc < 2:914868/143‑1 (MQ=255) gAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGTGTTCCGGATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCa > 1:381945/1‑143 (MQ=255) tGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGCCTATAGGACACGAGTCCCCGAGCAGGTc > 2:814883/1‑143 (MQ=255) tGATGGGCAGGACTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACtt < 1:477862/143‑1 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATAACGCGTAGGTGATGACCGACGCTCCGGCCGTGGCATCTACCGACTCTATGAAACCGTTCGCTGAGCCGGTCCGTACTTACGTACCGGc > 2:408160/1‑143 (MQ=255) tgtgcccGCTGGAGCATGCTGCACCCGCGGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTga < 2:368758/137‑1 (MQ=255) aaCGCTGGAACATGCTGCACCCGCCGCAATCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgacgac < 2:822152/143‑1 (MQ=255) cgggAGCATGCTGCACCCGCTGGAAACCCCGTGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCGACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc < 2:711766/141‑1 (MQ=255) cTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCACGTCCGTACTTACGTCCCGGCTCACGACTAcc > 2:731270/1‑143 (MQ=255) tGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAGCGGCTGACGACTACcg > 1:36359/1‑143 (MQ=255) gagcATTCTGCTCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAACCTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg < 2:70609/140‑1 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGGCTACcgcg > 1:790804/1‑143 (MQ=255) aaCATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGt < 1:745808/143‑1 (MQ=255) aCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACt > 1:941541/1‑143 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTATACCGCTTAGGTGATGAACGACGCGCCGGCAGTGACATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 2:558889/143‑1 (MQ=255) gCTTGAACCGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCCTCTACCGCCTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATg < 2:574099/143‑1 (MQ=255) cTCCGCGCGTTCCGTATATCGCTTAGGTGAGGAACGACGCTCCGGCAGTGGCATCTACCGACTATGTGAAAGTGTTCGCTGGGCAGGTCCGTACTTAGGTAACGGCGGACGACTACCGCGGCCTGGGTGGGGATGGGTTCGGt > 1:538927/1‑143 (MQ=255) tCCGCGCGTTCCGTATAGCGCTTAGGTGGTGCACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc < 2:224040/143‑1 (MQ=255) gccGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTCCCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc < 1:315443/142‑1 (MQ=255) cgcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCAGATGAAGTGGTTTGCGTGCTGGGTACTGATGGCTTCGGTCGTTc > 1:957259/1‑143 (MQ=255) atCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGAACGCCGTga > 2:1008902/1‑143 (MQ=255) | AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA > NC_000913/125247‑125523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |