| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 800,807 | G→T | 100% | A17S (GCG→TCG) | ybhH → | putative isomerase YbhH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 800,807 | 0 | G | T | 80.6% | 63.3 / 8.3 | 37 | A17S (GCG→TCG) | ybhH | putative isomerase YbhH |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base T (12/16); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCC > NC_000913/800667‑800941 | ttattaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAAGTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGctcg < 1:668805/143‑1 (MQ=255) taataATTGTTGCGCTAAAGTCAGTGGAGATTCTTTTCATTAAAGATAACCAGCTCGGCATCAGCGGTGTTTTTAAATTGAGGGCATTATTATTAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGt < 2:250542/140‑1 (MQ=255) aaccGTTGCGCTAAAGTAAATAGAGACGCTTTGCACTGAATGTAACCAAATACCAAAAAGATATGTATTTAAATAGAGAGCAGAATAAAAAAAAAAAGACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCt < 2:1000604/140‑1 (MQ=255) aTCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAGATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCCGGTTCCTg > 2:845353/1‑143 (MQ=255) aTCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTg < 1:367062/143‑1 (MQ=255) tCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAAATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGt < 1:388400/143‑1 (MQ=255) gcGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTCTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGt < 1:409952/142‑1 (MQ=255) ttGAGCTAAAGTCATTAGAGATGCTTTGCACTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTAGAGAAGCTCGTTCATGTTaa > 2:153350/1‑142 (MQ=255) tAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAAc < 1:546277/143‑1 (MQ=255) aaaGTCATGAGAGATGCTTTGCCCTTAATGCAACCATAGCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACa < 2:167398/143‑1 (MQ=255) aGTCATTGGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACATCGAGGGGCTCGTTCCTGTTAGTGGAACAtt > 1:339529/1‑143 (MQ=255) cATTAGAGATGCATGGCACTTAATGTAACCATAGCGCAATAAGTTATGTTTTTAAATCGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGCGGGGCTCGTTCCTGTTAGCGGAACATTTAc < 1:770332/143‑1 (MQ=255) ttAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGTTGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGCACATTTAccc > 2:195629/1‑143 (MQ=255) tttGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa < 2:19884/143‑1 (MQ=255) ttAAGGTAACCATATCGAAATATGTTATGCTTTTAAATTGAGGGCATTATTATGAAAAAAATAGCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgcg < 2:860749/143‑1 (MQ=255) ttaTGTCACCTTATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgcgc < 2:632020/141‑1 (MQ=255) tGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGAt > 2:658572/1‑143 (MQ=255) cGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt < 1:823722/143‑1 (MQ=255) cAATAAGCTATGCTTTTAAATTGCTGGCATTCTTATGCAAACAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATgg < 1:296746/143‑1 (MQ=255) aGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCCGCGCGATAAAATATTGGTGGCAAtt > 1:875924/1‑143 (MQ=255) tgaTGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTAt < 1:13566/141‑1 (MQ=255) tttttAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGGGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGGTCGATAAGATATTGATGGCAATTATGGGtt > 1:955419/1‑143 (MQ=255) tAAAGTGAGGGCATCACTACGAAACAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCgg < 2:161469/143‑1 (MQ=255) atggAGGGCATGATGAGGAAAAAGATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa < 2:636382/140‑1 (MQ=255) ttattattaAAAAAGTACCCTGCGGGATGTTGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACAGTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa < 2:11428/143‑1 (MQ=255) tattatGAAACAAATACCCTGCGTGATGATGCGGGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaaa < 1:326604/143‑1 (MQ=255) ttatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGTAACTCGGGGAGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCACACGCAGCGCGATCAAATATTGATGGCAATACTGGGCTCCGGTAACGCTCTGGagcgg > 2:828288/1‑139 (MQ=255) tGAAAAATATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGGAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAc < 2:742697/143‑1 (MQ=255) ggAAAATAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAc < 2:684527/142‑1 (MQ=255) aaaaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTCCCCGAAGCTCAAACGCAGCGCGATAAAATATTGATCGCAAATATCGGTTCCGGTAACGACCTGCAAATTAAccg > 1:814888/1‑141 (MQ=255) aaaaaaaTAACCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACAGTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGCTCTGGAAATTGCCgg > 1:700808/1‑143 (MQ=255) aCCCTGCGTGATGATGCGAGGCGTAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTggcg < 2:892693/143‑1 (MQ=255) gTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTaa > 1:22676/1‑143 (MQ=255) tgatgCGAGGTGGAACCTCGCGGGGCTCGTTCCTGTTAGCGGAACCTTTACCCGAAGATCAAACGAAGCGCAATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa > 1:10646/1‑143 (MQ=255) tgggAGGTGGACCCTCGGGGTGCCCGTTCCTGTTAGCGGAACATTTCCCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTg < 1:703761/140‑1 (MQ=255) gCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGa > 2:907307/1‑143 (MQ=255) gTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGAGCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGGAATTGACGGTATTGGCGGCGGTAATCCACTGAcgcgt > 1:681000/1‑139 (MQ=255) cGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGcc < 2:636882/143‑1 (MQ=255) | TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCC > NC_000913/800667‑800941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |