| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,499,361 | A→G | 10.1% | N142D (AAC→GAC) | rimL → | ribosomal‑protein‑L12‑serine acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,499,361 | 0 | A | G | 10.1% | 92.1 / 2.8 | 40 | N142D (AAC→GAC) | rimL | ribosomal‑protein‑L12‑serine acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (19/16); new base G (2/2); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01 | |||||||||||
TAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGC > NC_000913/1499224‑1499496 | tAGGCTACTGGCTGGACGAAGCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACGTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAg < 2:944360/143‑1 (MQ=255) ggCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGt < 1:850784/143‑1 (MQ=255) cTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGGATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCg > 1:909926/1‑143 (MQ=255) cTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCg > 1:64922/1‑143 (MQ=255) gctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgc < 1:210938/143‑1 (MQ=255) gctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgc > 2:274328/1‑143 (MQ=255) gctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgc > 1:750362/1‑143 (MQ=255) ctggACGGAGCGCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGTCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg < 2:846608/143‑1 (MQ=255) aCGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGGGGCCAATCGACGAAGCAAACAGGTCGCTTTGCGCCAt > 2:891743/1‑143 (MQ=255) aTCTCATCCGGGGCAGGGGATCATGTCTCAGGCGCTGCAGGCATTGATTCATCATTGCGCCCAGTCTGGTGAACGTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtt < 1:569077/143‑1 (MQ=255) tctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGCCACTGCGCAAAGCAACCAGGTCGCTTTGCGCAATGGttt > 1:33846/1‑143 (MQ=255) ctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCGACTGTCGCGGGGGCCATCCGCACAGCCACCAGGGCGCTTGGCGCAATCGtttt > 1:298752/1‑143 (MQ=255) gccggggcaccggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATcc < 1:243634/140‑1 (MQ=255) cggggcaggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCtt < 2:592161/142‑1 (MQ=255) accATGTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCt < 1:967592/141‑1 (MQ=255) aTTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGaa > 2:156701/1‑143 (MQ=255) tttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGaaa < 2:587834/143‑1 (MQ=255) ttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGCACTTAGACGCTTCGTGATCAAATGACGGATGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc > 1:797641/1‑143 (MQ=255) tGCAGGAATTGATTCATAATTCCGCAAAATCGAGGCAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTc < 1:421701/143‑1 (MQ=255) aTTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatg > 2:503282/1‑143 (MQ=255) atcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCACCGGTTTTCTCCTTGATGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCatat > 1:60540/1‑143 (MQ=255) aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgat < 2:293532/143‑1 (MQ=255) aCGCACTGTCTGGTCATCGTAGAGTTTTCGTGATGCATTGTGGTGTGGACACGCAGCAAAGTACCCTGGTCCCTTTGCGCATTGGTTTTACCCGATAAGGTTGCCTGAAACAGGCTGAGTTCCTGAAGCATGCCTatgatgat < 2:422319/143‑1 (MQ=255) gCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGCATGACGCCTATGATGCtgt > 1:40983/1‑143 (MQ=255) ccAGGCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGa < 2:565196/143‑1 (MQ=255) gTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGATCCTGAATGATGCCTAGGATGATGTGAAcg > 1:856998/1‑142 (MQ=255) tGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTAAGTTCCTGAATGATGACTATGCTGATGAGAACTtat > 2:352430/1‑143 (MQ=255) gggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCACTGGTCTTATCCTTGCAGGTTGCATGAATCAGGCTGAGTCCCTGAATGACGCCTATGATGATGTGAACTtat < 1:828482/142‑1 (MQ=255) gTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCGAAGTAACCAGGTCGCTTTGCGCAATGCGTTTATCCCTGAAGGTTGCCTGAAACACGCTGAGTTCCTGAATGCCGCCTATGATGCTGTGCACTtaccc > 2:217226/1‑140 (MQ=255) gAACTTAGACGCTTAGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATTGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgc < 2:979731/143‑1 (MQ=255) ttAGACGGTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCGTTGCGCAATGGTTTTAGCCTTGAAGGACGCCTGAGACAGGCTGAGTTCCCGAATGATGCCTATGATGATGTGAACCTATACGCGCGt < 2:721665/143‑1 (MQ=255) aCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtatta > 1:960120/1‑143 (MQ=255) ttCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGa < 1:769614/143‑1 (MQ=255) tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTGTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTca > 1:884968/1‑143 (MQ=255) aaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATaa < 1:274328/143‑1 (MQ=255) cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCggcggc > 1:14311/1‑143 (MQ=255) aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTAAATGATGCCTATGATGATGTGGACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgacgcg > 2:476490/1‑138 (MQ=255) tCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGtt > 1:187982/1‑143 (MQ=255) cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGCAGGTTGCCTGAAACAGGCTGAGTTCCTGACAGAGGCCTATGATGATGTGAACTTATACGCCCGTATTCTCGCGTCACAATGACCCTGCCAGCGGCGTTcg > 2:613473/1‑143 (MQ=255) gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCGGAAACAGGCTGAGTCCCTGAATGATGCCTTTGCTGATGTGCACTTATACGCGCGAATTCTCAATTCACAATAAAAGTGCCAacggcgataac > 1:513510/1‑138 (MQ=255) | TAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGC > NC_000913/1499224‑1499496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |