Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,657,593 T→C 8.9% L10P (CTT→CCT)  ynfD → DUF1161 domain‑containing protein YnfD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,657,5930TC 8.9% 103.3 / 2.7 45L10P (CTT→CCT) ynfDDUF1161 domain‑containing protein YnfD
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base C (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

TTTCACAACAGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTT  >  NC_000913/1657452‑1657735
                                                                                                                                             |                                                                                                                                              
tttCACAACAGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCtt                                                                                                                                               >  1:591912/1‑143 (MQ=255)
 ttCACAACAGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGTCACAAGAAGTTATCTTCGCGTCAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTc                                                                                                                                              >  1:377342/1‑143 (MQ=255)
  acacaACAGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCt                                                                                                                                             <  2:43804/142‑1 (MQ=255)
    acaacaGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGc                                                                                                                                           >  2:635623/1‑143 (MQ=255)
      aacaGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTACATGCTCTGCTTAACGAAGTTATCTTCGCGTCAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGATTCTGctc                                                                                                                                         >  1:20168/1‑143 (MQ=255)
      aacaGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGctc                                                                                                                                         <  2:905511/143‑1 (MQ=255)
              acAGAGAGAAGAGAGGATTCTCAAACATAGTAGCAAAGACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGctcgccctcgc                                                                                                                                 <  1:164058/142‑1 (MQ=255)
               cAGAGCGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTCAATGCTCTGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTCTGCCGCGCTTCTTctcgccctcgcc                                                                                                                                >  2:333023/1‑143 (MQ=255)
                  agagTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAGCCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCa                                                                                                                             <  1:990505/143‑1 (MQ=255)
                         agaTGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTAACCcgcgc                                                                                                                      >  1:213717/1‑142 (MQ=255)
                                  tcAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTcgccg                                                                                                             <  1:196196/143‑1 (MQ=255)
                                   cAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTAAGATAAGTTATCTCCGCGTAACGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTcgccgc                                                                                                            <  1:961296/143‑1 (MQ=255)
                                    aaTCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCCCCCGCGGTACTcgccgcg                                                                                                           >  1:229874/1‑143 (MQ=255)
                                    aaTCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCCTCTGCTCGCCCTCGCCTCACCCGCGGTACTcgccgcg                                                                                                           >  1:569328/1‑143 (MQ=255)
                                       cATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGAAACTctacgcgccg                                                                                                        >  1:627759/1‑143 (MQ=255)
                                          agtagCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGa                                                                                                     <  2:541478/143‑1 (MQ=255)
                                           gtagCAAATACAGTACTTTACACGTTAAATTCTATGCTTAAATAAGTTATCTTCTCTTATGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGAt                                                                                                    <  1:801228/143‑1 (MQ=255)
                                                  aTACAGTACTTTACACGTTAAATGCTATGCTTAAAGTAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGa                                                                                             <  2:328892/143‑1 (MQ=255)
                                                  aTACAGTACTTCACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGa                                                                                             <  2:11011/143‑1 (MQ=255)
                                                   tACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGCTCCtgcgcg                                                                                            >  2:703759/1‑139 (MQ=255)
                                                        tACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGCTCCCGTGAGCGCAt                                                                                       >  2:25919/1‑143 (MQ=255)
                                                        tACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCCCCGGCACCCTGTCCgcgcgt                                                                                       >  2:458558/1‑141 (MQ=255)
                                                                  gTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGat                                                                             <  1:851697/143‑1 (MQ=255)
                                                                     aaaTGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCCGCTCGCCCGCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGatata                                                                          <  2:408027/143‑1 (MQ=255)
                                                                      aaTGCTATGCTTAAAGAAGTTATCTTCGAAAAAGGCGACTAGGAGTAAACGCTCAACCTGATGTGCCGCGCTTCTGCTCGCCATCGCCTCACCCGCGGTACTCGCCGCGCCGGGGTCCCGCGAGCGGATACAGAGCGatatag                                                                         >  1:194005/1‑142 (MQ=255)
                                                                                                  cgTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATCATCAACAATGGCGTAcc                                             >  2:776727/1‑143 (MQ=255)
                                                                                                  cgTAAGGAGCTTATGATGAAACTCTCAACCTGCTGGGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTAcc                                             <  1:107972/143‑1 (MQ=255)
                                                                                                      aGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGaa                                         <  2:179204/143‑1 (MQ=255)
                                                                                                       ggAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCCTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGaaa                                        <  2:569328/143‑1 (MQ=255)
                                                                                                       ggAGCTTATGATCAACCTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGaaa                                        <  1:27791/143‑1 (MQ=255)
                                                                                                             tATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCCTACAGCGCGATCTATCACAGCGCACTATCAATAATGGCGTACCCCAAAGCAGCt                                  >  1:766723/1‑143 (MQ=255)
                                                                                                               tgatgaACCTCTCATCCTGCCGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTc                                <  2:591912/143‑1 (MQ=255)
                                                                                                                 atgaAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCAc                              <  1:666836/143‑1 (MQ=255)
                                                                                                                   gaAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGGGATATATCACAGCGCGTTATCAGTCATGGCGTGCCCGACAGCAGCTTcgcgc                            >  1:768845/1‑142 (MQ=255)
                                                                                                                          tcAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTAt                     >  1:575052/1‑143 (MQ=255)
                                                                                                                          tcAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTAt                     <  1:255100/143‑1 (MQ=255)
                                                                                                                            aaCCTGCTGTGCCGCGCTTCTGCTCGCCCTCGGCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTg                   <  2:40101/143‑1 (MQ=255)
                                                                                                                            aaCCTGCTGGGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTg                   <  1:876246/143‑1 (MQ=255)
                                                                                                                             aCCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGCAAGCAGCCTCACGTTCAGTATTGt                  >  2:912505/1‑143 (MQ=255)
                                                                                                                                    gtgCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAGGTATTGTACCCAAt           >  1:409067/1‑143 (MQ=255)
                                                                                                                                    ggggggcgcTTCGGCGCGCCCTCGCGTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAAt           <  2:653181/138‑1 (MQ=255)
                                                                                                                                        cgcgCTTCTGCTCGCCCTCGCCTCACCCGCGGAACTTGCCGCGCCGGGCTCCTGTGAGCGCATACAGAGCGATAGATCACAGCGCGTTATCAATAGTGGCGTACCGGAAAGCAGCTTCAAGGTAAGGATTGGACACAATGAcc       >  1:146516/1‑143 (MQ=255)
                                                                                                                                        cgcgCCTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCg                                                                               <  1:971992/71‑1 (MQ=255)
                                                                                                                                        cgcgCCTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCg                                                                               >  2:971992/1‑71 (MQ=255)
                                                                                                                                          cgcTTCTGCTCGCCCTCGTCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAg     <  2:793761/143‑1 (MQ=255)
                                                                                                                                          cgcTTCTGCCCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAg     <  1:718901/143‑1 (MQ=255)
                                                                                                                                             ttCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAACGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGAAATATACCCAATCACAAgcat  >  2:36782/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TTTCACAACAGTCACCAGAGAGTAGAGATGATTCTCAATCATAGTAGCAAATACAGTACTTTACACGTTAAATGCTATGCTTAAAGAAGTTATCTTCGCGTAAGGAGCTTATGATGAAACTCTCAACCTGCTGTGCCGCGCTTCTGCTCGCCCTCGCCTCACCCGCGGTACTCGCCGCGCCGGGATCCTGTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTT  >  NC_000913/1657452‑1657735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑