| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,567,377 | A→G | 8.2% | V244A (GTG→GCG) | eutH ← | putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,567,377 | 0 | A | G | 8.2% | 141.0 / 3.6 | 59 | V244A (GTG→GCG) | eutH | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base G (2/3); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01 | |||||||||||
TCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATCACCGGGATCATGTT > NC_000913/2567240‑2567516 | gcGGATACGCCCCTAACTGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCGCCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTAcc < 2:705911/142‑1 (MQ=255) cGGATACGCCCCTAACAGAACGCAGGAGACAGAACAGATAATGTCAATGGCGCGCAGCACCTGACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCg < 1:514226/143‑1 (MQ=255) aCGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCAGGTTTATCGCCAGGGGCCATAAAGATAGGATCCACACCGGGGACCAGTTCCCCGCCAAGCAGGAATTTCACTCCCCGAGCg > 2:631719/1‑143 (MQ=255) aCGCCCCTAACAGAACGCAGGAGAGAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCg < 1:552628/143‑1 (MQ=255) cGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATAACCTCACCGGGTTTATCGCCAGGGGCCGTAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTGCACTACCGCAGAgg > 1:344876/1‑143 (MQ=255) ccTCACGGAACGCAGGAGTTAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGTGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg < 2:453286/143‑1 (MQ=255) ccTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCGATAAAGATAGGCTAAAGCTCAGTTCGCAGTTCCCAGCCAAGCAGGAACTTCACTCCCGCCGCGGCAAg > 1:426861/1‑143 (MQ=255) ccTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGGTCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGGATTCCACTACCGCAGCGGCCAg > 1:348320/1‑143 (MQ=255) ccTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTACACTAGCGCTGCGGCAAg > 1:628164/1‑143 (MQ=255) tAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCCTCAAGATAGGATCCAGCCCGGGGATCAGTTCCCAGCCAAGCAGTAATATCACTACAGCAGCGGCAAGAc > 1:6027/1‑143 (MQ=255) aaCAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGAcc > 1:509867/1‑143 (MQ=255) aGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCCTAAAGCTAGGATCCAGACCGCGCATCAGTTCCCCCCCAAGCAGGACTTTCACTACCCCCGCCGCACGACCTAg > 1:247082/1‑143 (MQ=255) aGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCGCTACCGCAGCGGCAAGACCGAg > 2:515793/1‑143 (MQ=255) aGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCGCTACCGCAGCGGCAAGACCGAg > 2:399678/1‑143 (MQ=255) aaCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAggg > 1:982874/1‑143 (MQ=255) aaCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAggg > 1:711715/1‑143 (MQ=255) ccGCAGGAGATGGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGt < 2:32665/142‑1 (MQ=255) agaTAGAACCGATAACTTAAATGGCGCGCAAGACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAAt < 2:1017186/143‑1 (MQ=255) tAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGCCCGAGGGTGATCAATGCa > 1:853169/1‑143 (MQ=255) aGAAGCGCTAACGTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCaa < 2:242210/143‑1 (MQ=255) ccGATAAGTTCAATGGCGCGCATAACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAg < 1:289080/143‑1 (MQ=255) aCTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAttt < 2:711715/143‑1 (MQ=255) aCTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGCTCCCAGCCAAGCAGGAAGTTCACTACCGCAGCGGCAAGACCGAGGGTGATCACTGCAACGAGGCAttc > 2:648458/1‑142 (MQ=255) ttCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAACGCTAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 2:453504/1‑143 (MQ=255) tCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGc > 2:776010/1‑143 (MQ=255) ggatcgcATCTCCTCACGGGGTTGATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGa < 2:795580/139‑1 (MQ=255) cgcgcgcATCACCGCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGAGCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGa < 2:458506/142‑1 (MQ=255) tggaTCACCTCACCGCGTGTGTTGCGAGGGGCCATAGAGATAGGCTCAGGACCAACTAGGAGTTCCCTGCCAAACAGGAATTTCACTACCGCAGCGGCAAGAAGGAGGGTGTTCAATGCAACGAGGAATTTGGCGAAGATCTg < 2:450071/140‑1 (MQ=255) gcctcaccgcaccGGGTTTATCGCCAGGGGCCATCAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTgg < 2:235610/140‑1 (MQ=255) gcATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGTGAAGATCTgg < 2:509867/143‑1 (MQ=255) aTCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGaa > 1:413182/1‑143 (MQ=255) accGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGa > 1:946579/1‑143 (MQ=255) cGGGTTTATCGCCAGGGGCTATAAAGAGAGGATCCAGACGGGGGATCAGTTCCCAGCCAAGCAGGAATTTCGCGACCGCAGCGGCAAGGCCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATc < 1:974349/143‑1 (MQ=255) ggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTAACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAATTGGCGAAGATCTGGAAGCCGTTGACCAt > 1:271282/1‑143 (MQ=255) ggttACCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCAtt < 1:766593/141‑1 (MQ=255) gTTGATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGTAATTTTAATTTCGCATCTGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCAtt < 2:359019/143‑1 (MQ=255) gCCAGGGGCCATAGAGATAGGATCCAGACCGGGGATCAGTGCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCg < 1:410318/143‑1 (MQ=255) aGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCCGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGACGCCGTTGATCATTTTTTCCgggg > 1:384022/1‑142 (MQ=255) tAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTc < 1:776010/143‑1 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCGCTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCa < 1:515793/143‑1 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCGCTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCa < 1:399678/143‑1 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCa > 1:300336/1‑143 (MQ=255) aaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGATGATCATTTTTTCCGGGATGAATTTCAg > 2:520472/1‑143 (MQ=255) gATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTGAGcg > 1:782706/1‑142 (MQ=255) gATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGcc > 1:812222/1‑143 (MQ=255) ccGGGGAGCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAg < 2:228521/143‑1 (MQ=255) aTCAGTTCCCAGCCAAGCCGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTTGAAGCCGTTGATCATTTTTTCCGGGATTATTTTCAGCCCCAGCGCCACCAGAATCGc < 1:526218/143‑1 (MQ=255) gcAGTTCCCAGCCAGGCAGGAATTTCACTACCGCGGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGca < 2:348320/142‑1 (MQ=255) aGTTCCCTGCAAAGCAGCAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGcaac < 2:331251/143‑1 (MQ=255) gTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGcaaca > 1:26705/1‑143 (MQ=255) gtCCCAGCCAAGCAGGAATTTCCCTGCCGCAGGGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGcaacaa < 2:813619/142‑1 (MQ=255) aGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATc < 2:628164/143‑1 (MQ=255) aGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATc < 1:453504/143‑1 (MQ=255) aGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAACGCCACCAGAATCGCAACAATGATc > 2:913028/1‑143 (MQ=255) cAGGAATTTCACTACCTCATTGTTAAGTTCTAGTGTGTTTAATGTAATGAGGAATTTGGCGAAGATCTGGAAGCCGTTGAGCATTTTGTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATCACCGGGa < 1:763354/143‑1 (MQ=255) agttCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATCACCGGGATCATg < 1:881417/141‑1 (MQ=255) tttCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGCTCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATCACCGGGCTCATGt > 1:449612/1‑143 (MQ=255) gttCACGCCCGCGGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGACCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATCACCGGGATCATGt < 2:26478/142‑1 (MQ=255) ttCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTCTTTCCGGGATGCATTTCAGCCCCACCGCCACCCGAATCGCAACAAGGATCACCGGGCTCATGtt > 1:650785/1‑143 (MQ=255) | TCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGCGCCACCAGAATCGCAACAATGATCACCGGGATCATGTT > NC_000913/2567240‑2567516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |