Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG100.0% 105.4 / ‑6.7 38V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (15/21);  minor base A (1/0);  total (17/21)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661835‑2662107
                                                                                                                                      |                                                                                                                                          
cTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCATAACAGATAACCACCACCTGGTCCACGCCCGCCGCACAACAGACCAt                                                                                                                                    >  2:602668/1‑143 (MQ=255)
 tGGCAGCCGCCTTTACCCTGACAACGCCTTGCATCTACAGATTCGTCAACCCCGCTAATTACTTCGCCAACGGCGCTGCTGCTGGCATCTTTGCCTAACAGATAACCCCCGCCTGCTCCACGTACGCTGGCAACCAGACCAtc                                                                                                                                   >  2:124712/1‑142 (MQ=255)
      gcagccTTTTACCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                              <  1:447790/140‑1 (MQ=255)
           cTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGa                                                                                                                         >  1:934991/1‑143 (MQ=255)
              tACCGTGAGTACGGGGGGCATCTACAGATTCGGCAACGGCGCTAATTACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACgg                                                                                                                      <  2:469103/143‑1 (MQ=255)
                 ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCCTTTTTACGCAGACgggta                                                                                                                   >  1:91553/1‑141 (MQ=255)
                   tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaaa                                                                                                                 <  2:753999/143‑1 (MQ=255)
                        aCGGGTGGCATCTACAGCTTCGTCAACGGCGCGAAATACTTCGCCTACTGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgtt                                                                                                            <  1:228221/143‑1 (MQ=255)
                          gggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgttgt                                                                                                          <  1:517880/143‑1 (MQ=255)
                            gTGGCATCTACAGATTCGTCAACGGCGCTAATTACCTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTc                                                                                                        <  1:967563/143‑1 (MQ=255)
                                    tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAg                                                                                                >  1:691369/1‑143 (MQ=255)
                                               ctaCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAtt                                                                                     <  2:637722/141‑1 (MQ=255)
                                                aaCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCGGACCATTTTTACGCAGACGGGAGAAGAGTGGGTCCCGGTAAGAGAGGGAAAtgc                                                                                    >  2:610511/1‑141 (MQ=255)
                                                   ggCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                 >  1:140785/1‑143 (MQ=255)
                                                     cgcTAATTACTTCGCCAACCGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGa                                                                               <  2:260804/143‑1 (MQ=255)
                                                          cttACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCCCCGCTCAGAGCGGGTTCGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTc                                                                          <  1:468932/142‑1 (MQ=255)
                                                               ttCGCCAACGGCGATGCTGCTGGCCTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGGTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                     <  1:985211/143‑1 (MQ=255)
                                                                   ccAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGACAACAGTTGTTCCAGACAAGAAAGGGAAAGTGCCTGACGTGCGGAAATATc                                                                 >  2:657397/1‑143 (MQ=255)
                                                                    cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCGTTTTTACGCAGACGGGAAAACAGTTGGTCCAGATAAGAAAGGGAAATTCGATGACGTTCGGAAACATCa                                                                >  2:764223/1‑143 (MQ=255)
                                                                           gATGCTTCTGGCATTTTTGCTGTACAGATAACCGTCGCGTGGTTCTCGTACGCTGTAAAGCAGACCATTTTTGCGTAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGACATTCTCTGACGTTCGGAAATATCAGCCAACg                                                         <  2:296387/143‑1 (MQ=255)
                                                                                tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTAcc                                                    <  1:3176/143‑1 (MQ=255)
                                                                                 gctgGCATCTTTGCCTAACAGATACCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCg                                                   <  1:305409/143‑1 (MQ=255)
                                                                                   tgGCATCTTTGCCTAACAGATAACCGCCGCCGGGTCCACGTACGCTGGAACGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg                                                 <  1:752245/143‑1 (MQ=255)
                                                                                   tgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACAggg                                                 >  1:748234/1‑143 (MQ=255)
                                                                                    ggCATCTTTGCCTAACAGATAACCACCGCCTGGGCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGc                                                <  1:876139/143‑1 (MQ=255)
                                                                                     gCATCTTTGCGTAACAGGTAACCACCGCCGGGTCCACGGACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGcc                                               <  1:424521/143‑1 (MQ=255)
                                                                                              ccTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                      <  1:289053/143‑1 (MQ=255)
                                                                                                tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt                                    >  2:135808/1‑143 (MQ=255)
                                                                                                    aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCCTCAGAGTTgcg                                >  2:786563/1‑141 (MQ=255)
                                                                                                          ccaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATCCAAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAAc                          >  1:423561/1‑143 (MQ=255)
                                                                                                          ccaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTCCGGAAATATCCCCGCGCTGTACCGGGCCCGCTTCAGAGTTGAGCGCagc                          >  2:714445/1‑141 (MQ=255)
                                                                                                             ccttcTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTc                       <  1:817103/139‑1 (MQ=255)
                                                                                                               gcgtGGTGCACTGACGCTGGACAGCAGACCATTTTTACGCAGACCGGAAAACAGTTGTTCCCGTTAAGAATGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCaa                     <  2:569560/140‑1 (MQ=255)
                                                                                                                ccTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAACCAGTTGTTCCAGATAAGAAAGGGAAATTCCATGACGTTCGGAAATATCAGCCAACGGTACTGGGCCCGCTTGAGAGTTTCGCGGAACGCCacg                    >  1:629005/1‑141 (MQ=255)
                                                                                                                   ggTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGATGAGCGCAAGGTCCAGCAt                 >  2:683866/1‑143 (MQ=255)
                                                                                                                     tCCACGTATGCTCGAAAGCAGACCATTTTTACGCAGACGGGAAACCAGTTGTTCCAGATAAGAAAGTGAACTTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg               <  2:805049/143‑1 (MQ=255)
                                                                                                                       cactggcGCTGGATAGCAGCCCATTTTTACGCCGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCACCGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCg             <  1:285241/137‑1 (MQ=255)
                                                                                                                                  gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTAAAGCATTGCGGTCACGGCATa  >  1:574602/1‑143 (MQ=255)
                                                                                                                                      |                                                                                                                                          
CTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661835‑2662107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑