| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,136,312 | C→A | 7.2% | L20L (CTG→CTT) | pitB ← | metal phosphate:H(+) symporter PitB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,136,312 | 0 | C | A | 7.2% | 142.5 / 4.3 | 56 | L20L (CTG→CTT) | pitB | metal phosphate:H(+) symporter PitB |
| Reads supporting (aligned to +/- strand): ref base C (27/25); new base A (2/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01 | |||||||||||
AATAACACGCCAAAAAAGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCAAAGTGAAATATCATTTT > NC_000913/3136172‑3136454 | aaTAACACGCCAAAAAAGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCag > 1:648622/1‑143 (MQ=255) cacGCCAAAAAAGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGACACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCATagcca > 2:850809/1‑141 (MQ=255) acGCCAAAAAAGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGagcaag > 2:261049/1‑143 (MQ=255) acAAAAAAGTTAAGAACGGCGGCCATCACCTCAGGAAGTGGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAgcaagcaa < 2:567794/142‑1 (MQ=255) gaaaaaaGTTAAAAAAGACCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAAt < 2:992386/142‑1 (MQ=255) aaaaaaGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAACAAGCAATa > 2:836078/1‑143 (MQ=255) aaaGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATAGATTGCTCCGTAGAACAACACAAATGCCAGCGCAAGCAATAACa > 2:699848/1‑143 (MQ=255) gtcaaaaaaAGCTTCCATCTCCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAACAAGCAATAACaaaa < 1:134256/140‑1 (MQ=255) ttAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAg > 1:751268/1‑143 (MQ=255) ttAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAg > 2:180226/1‑143 (MQ=255) ttAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCAAGAGCAAGCAATAACAAAAg > 2:592312/1‑143 (MQ=255) ttAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCAAGAGCAAGCAATAACAAAAg > 2:99820/1‑143 (MQ=255) tAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTAGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGc > 1:70208/1‑143 (MQ=255) aaaaaaTGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGcc > 1:50818/1‑143 (MQ=255) aaaaaaTGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAAACACTGATAGCTTCGCAGAACAACACAAATGCCAGCGCAAGCAACAACAAAAGcc > 2:401636/1‑143 (MQ=255) aaaaaTGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGccc > 1:641848/1‑143 (MQ=255) aaaaTGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCt < 1:206215/143‑1 (MQ=255) aaTGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCtgt < 1:758545/143‑1 (MQ=255) gctgccATCACGAGAGCAGGTTGTGCGGGCATGGCATGATTATAAAGAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCGTTGAATGATTTGAAGAATAAGACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGta < 2:454101/140‑1 (MQ=255) gccgccATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAGCGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGta < 2:47505/143‑1 (MQ=255) cgccATCACCACAGCAAGTGGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGtata < 2:30735/143‑1 (MQ=255) cacaGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAgga > 1:318994/1‑142 (MQ=255) gTTGTGGTTGCATGGCACGAGTATAAATAATGGCTGCTACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAAGAACAAAAGCCCTGTGTATATATCAAGGCCAACaaat < 1:718799/143‑1 (MQ=255) ggTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCAAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAAtt < 1:592312/143‑1 (MQ=255) ggTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCAAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAAtt < 1:99820/143‑1 (MQ=255) gTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAAttt > 1:391671/1‑143 (MQ=255) gTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAAAACAAATGCCAGAGCAAGCAATAAAAGAAGCACTGTGGATATATCACGGCCAACAAAAAAAatt > 2:871693/1‑143 (MQ=255) ttGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGAACTGAGAGAAAACCTGTGTATATATCAAGGCCAACAAATAAAATTa > 2:982761/1‑143 (MQ=255) tGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAATAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAg < 2:973603/143‑1 (MQ=255) aTGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCat < 1:213381/143‑1 (MQ=255) ggCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCatat < 2:152199/143‑1 (MQ=255) cACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGCACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAGATAAATATAGCatatat > 2:543316/1‑143 (MQ=255) ataGTAGAAATAACGACTGACACCGAATTCGTCGAATCATGAAAACCATTGATATCTACATAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCTCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTa < 2:545181/141‑1 (MQ=255) cGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCAttt > 1:370811/1‑143 (MQ=255) ggCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGCAGAACAACACAACTGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATCTATTAGATTACGCGAtgtt > 2:53927/1‑143 (MQ=255) cTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGa < 1:261049/143‑1 (MQ=255) cTGCCAACGCATTCGCAGTGTCATGAAAACCATTGATTTCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATGTAGCATATATTAGATTACGCCATTTTGa < 2:635236/143‑1 (MQ=255) tGCCACCGCATTCGCCGGGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGaa < 2:127811/143‑1 (MQ=255) aCCGCATGCGCCGTGTCATGAAAACCATTGCTGGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAGAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTa < 1:80525/143‑1 (MQ=255) aTTCGTGGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAAc < 2:862461/143‑1 (MQ=255) tCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACgg > 1:681621/1‑143 (MQ=255) gCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATCACAAAAGCCCTGTGTATATATCAAGGCCAACAAATACGTTTAGGCTAGATCAGATTACGCCATTTCGGAGTTACGAAgggct > 1:158765/1‑141 (MQ=255) tCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTa > 2:236975/1‑143 (MQ=255) cATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTAt < 1:505308/143‑1 (MQ=255) tGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCa < 1:986725/143‑1 (MQ=255) aaaaCCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGt > 1:881325/1‑143 (MQ=255) acccATTGATTGCGTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGa < 1:260867/141‑1 (MQ=255) ttgattgCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTa > 1:856138/1‑143 (MQ=255) tgCTTCGTAGAACAATACAAATGCCAGAGCATGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGc < 2:1014065/143‑1 (MQ=255) cTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCAt > 1:603629/1‑143 (MQ=255) aGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCaa > 2:171240/1‑143 (MQ=255) gAACAACACAAATGCCAGAGCAAGCAATAACAAAGGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCaaa < 2:580828/143‑1 (MQ=255) acacAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCAACGAGaa > 2:793222/1‑143 (MQ=255) aaTGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGTTTTTATTATCGGACGCATTATCAGTGACTTTAACGTCATGGGCAAAGTGAAATATc > 2:635278/1‑143 (MQ=255) aaTGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCAAAGTGAAATATc < 2:552795/143‑1 (MQ=255) gCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCAAAGTGAAATATCAtt < 1:982761/143‑1 (MQ=255) ccAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTAAGCCATTTTGAATTTACGAACGGACGCATTATAAGTGACTTTAACGGTATGGGGAAAGTGAAATATCAttt > 2:491175/1‑143 (MQ=255) cAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCAAAGTGAAATATCAtttt < 2:681621/143‑1 (MQ=255) | AATAACACGCCAAAAAAGTTAAAAAATGCCGCCATCACCACAGCAAGTTGTGGTTGCATGGCACGAGTATAAATAACGGCTGCCACCGCATTCGCCGTGTCATGAAAACCATTGATTGCTTCGTAGAACAACACAAATGCCAGAGCAAGCAATAACAAAAGCCCTGTGTATATATCAAGGCCAACAAATAAATTTAGCATATATTAGATTACGCCATTTTGAATTTACGAACGGACGCATTATCAGTGACTTTAACGGCATGGGCAAAGTGAAATATCATTTT > NC_000913/3136172‑3136454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |