Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,347,170 G→A 7.0% R19H (CGT→CAT)  rapZ → RNase adaptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,347,1700GA 7.0% 161.0 / 2.0 59R19H (CGT→CAT) rapZRNase adaptor protein
Reads supporting (aligned to +/- strand):  ref base G (22/32);  new base A (2/2);  total (24/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

AAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAA  >  NC_000913/3347029‑3347309
                                                                                                                                             |                                                                                                                                           
aaaTCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCtgcgt                                                                                                                                            >  1:577162/1‑143 (MQ=255)
   tCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCtgcgtgcg                                                                                                                                         >  2:498877/1‑143 (MQ=255)
     aTTACGGAGACCGAAGGGACTCCGGATGAAGCGTAGGTAGGCGGTAAGGTCTCTTTTAGACGGTGTGAGGAGAAACAGTACATGGTACTGATGAGCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCt                                                                                                                                       <  1:98615/143‑1 (MQ=255)
         cGGATGCCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGaa                                                                                                                                   <  2:318534/143‑1 (MQ=255)
         cGGATACCGAAGGTACTCCGGAGGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGaa                                                                                                                                   <  1:331331/143‑1 (MQ=255)
            aTACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGat                                                                                                                                >  2:199683/1‑143 (MQ=255)
                cggaGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATgg                                                                                                                            <  2:877017/140‑1 (MQ=255)
                cGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATgg                                                                                                                            >  1:379252/1‑143 (MQ=255)
                   aGGTACCCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGtt                                                                                                                         <  1:320110/143‑1 (MQ=255)
                    ggTACTCCGGATGAAGCGTAGTTAGTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCACATAAGTCAGACAGCCTGCGTGCGCTGGAAGATATGGGttt                                                                                                                        <  2:313629/143‑1 (MQ=255)
                          ccGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTCAATCTGTCGCCCTGCGTGCGCTGGCAGGTATGGGTTTTTACta                                                                                                                  >  1:782278/1‑142 (MQ=255)
                          ccGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCATGCGCTGGAAGATATGGGTTTTTACTg                                                                                                                  >  2:699283/1‑143 (MQ=255)
                          ccGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCATGCGCTGGAAGATATGGGTTTTTACTg                                                                                                                  >  2:699394/1‑143 (MQ=255)
                           cGGAGGATGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTAGTTGGTACTGATGAGCGTCAGCGGACTTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGc                                                                                                                 <  2:705806/143‑1 (MQ=255)
                             gATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGATCGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGt                                                                                                               <  1:190222/143‑1 (MQ=255)
                             gATGAAGCGTAGGTATTCGGTACTGTCTCTTTTAGACGTTGTGAGGAGGAACAGTACATGGTACCGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGt                                                                                                               <  2:304750/143‑1 (MQ=255)
                              aTGAAGCGTAGTGATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTg                                                                                                              <  1:751969/143‑1 (MQ=255)
                                 aaGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTGTTTACTGCGTGGag                                                                                                           >  2:685554/1‑142 (MQ=255)
                                  aGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGACATGGGTTTTTACTGCGTGGATa                                                                                                          >  2:515383/1‑143 (MQ=255)
                                    cGTAGTTATTCGGTAATGTCTCTTGTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAAc                                                                                                        <  2:1004736/143‑1 (MQ=255)
                                     gTAGTTATTCGGTAATGTCTCATTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGACGATATGGGTTTTTACTGCGTGGATAAcc                                                                                                       >  2:218398/1‑143 (MQ=255)
                                         ttATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGCAGATATGGGTTTTTACTGCGTGGATAACCTTcc                                                                                                   >  1:641005/1‑143 (MQ=255)
                                          gagtCGGTAAGGTCTGGTTTAGACGCTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTccc                                                                                                  <  1:285327/140‑1 (MQ=255)
                                            ttCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGt                                                                                                <  1:730016/143‑1 (MQ=255)
                                              cGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATCACCTTCCCGTAg                                                                                              >  2:252128/1‑143 (MQ=255)
                                               gttAATGTCTCTTTTATACGTTGTGAGGAGAAACAGTACTTGTTACTGATGATCGTCAGCGGACGTTCAGGTTCAGTTAATTCTGTCGCCCTGCGTGCGCTGGAAGATATGAGTTTTTACTGCGTGTTTAACCTTCCCGTAgt                                                                                             <  2:28243/141‑1 (MQ=255)
                                                 tAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAgtgt                                                                                           <  1:498877/143‑1 (MQ=255)
                                                    tgtccattttAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTGAGAGCACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGtgttgt                                                                                        <  1:117920/137‑1 (MQ=255)
                                                        tctTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTAcc                                                                                    <  2:527221/143‑1 (MQ=255)
                                                        tctTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTAcc                                                                                    >  2:739773/1‑143 (MQ=255)
                                                        tctTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTAcc                                                                                    >  2:803111/1‑143 (MQ=255)
                                                        tctTTTAGACGTTGTGAGAAGAAACAGTACATGGTAATGATGAACGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTATATAATAAGTAGATAACATTCCCACTGAGTTGTTAcc                                                                                    >  2:111030/1‑143 (MQ=255)
                                                             tAGGCGTTGTGAGGAGAATCAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATc                                                                               <  2:1000970/143‑1 (MQ=255)
                                                             tAGGCGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATc                                                                               <  1:924623/143‑1 (MQ=255)
                                                                aCGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCATGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTgg                                                                            <  1:699394/143‑1 (MQ=255)
                                                                aCGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCATGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTgg                                                                            <  1:699283/143‑1 (MQ=255)
                                                                  gTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCt                                                                          <  1:142057/143‑1 (MQ=255)
                                                                       acgacgaAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAAc                                                                     <  1:780675/138‑1 (MQ=255)
                                                                          gagaAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCt                                                                  >  1:171756/1‑143 (MQ=255)
                                                                            gaAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTCCCCGATCTGGCTCGAACCCTgg                                                                >  1:379926/1‑143 (MQ=255)
                                                                            gaAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTgg                                                                <  2:186272/143‑1 (MQ=255)
                                                                               aCAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCg                                                             >  2:188140/1‑143 (MQ=255)
                                                                                     cATGGTCCCGATGATCGTCAGCGGACCTTCAGGTTCAGGTAAATCTGACGCCCTGCGTGCGCTGGAAGCTATGGGTTTTTACTGCGTGGATAACCTCCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATcgcg                                                       <  1:475947/143‑1 (MQ=255)
                                                                                        ggTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCgaga                                                    <  1:913079/143‑1 (MQ=255)
                                                                                            cTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTc                                                >  1:487271/1‑143 (MQ=255)
                                                                                              gatgatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGCTTTCTg                                              >  1:984337/1‑143 (MQ=255)
                                                                                              gatgatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTg                                              >  2:200602/1‑143 (MQ=255)
                                                                                              gatgatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTg                                              <  1:210570/143‑1 (MQ=255)
                                                                                                tgatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGTTGGAAGATATTGGTTTTTACTGTGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgcc                                            <  1:218398/143‑1 (MQ=255)
                                                                                                tgatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGGTTTTACTGCGTGGATAACCTTCCCGTAGCGTCGGTGCCCGATCTGGCTCGAAACCTGGCCGATCGCGAGATTGCTgcc                                            >  1:960602/1‑143 (MQ=255)
                                                                                                 gatCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTgccg                                           >  1:470182/1‑143 (MQ=255)
                                                                                                              cGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTATTATGTTTTTATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGa                              <  1:739773/143‑1 (MQ=255)
                                                                                                                   aggttcaggtAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTc                         <  1:188140/143‑1 (MQ=255)
                                                                                                                           gtAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTAGTGAGTGGATAACGTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATCTCTCCCGCCGTCAGCATTGATGTTCGTACTATg                 >  1:746310/1‑143 (MQ=255)
                                                                                                                           gtAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGGGTTGTTAGCCGATCTGGCTCGAACCCTGGCCGATCGCGAGCTTTCTGCCGCCGTCAGCGTGGGCGTTCGTGAtgtg                 >  2:745709/1‑143 (MQ=255)
                                                                                                                             aaaTCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGcc               <  1:200602/143‑1 (MQ=255)
                                                                                                                              aaTCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCg              <  1:286893/143‑1 (MQ=255)
                                                                                                                                tCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGa            <  2:577162/143‑1 (MQ=255)
                                                                                                                                gcTGTCGCCCTGCCTGCGCTGGAGGATATGGGTGTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGa            <  1:152769/142‑1 (MQ=255)
                                                                                                                                       cccTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTGCCGTAGTGTGGTTACCCGATGTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAACATTGATGTTCGTAAGATGCCCGAGGCACCa     >  2:631358/1‑143 (MQ=255)
                                                                                                                                         cTGCGTGGGCGGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGAGCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGa   <  2:704728/143‑1 (MQ=255)
                                                                                                                                          ggcgtgcgGTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTCCCCGATCTGGCTCGAACCCTGGCCGCTCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGaa  <  1:93811/142‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
AAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCTCGAACCCTGGCCGATCGCGAGATTTCTGCCGCCGTCAGCATTGATGTTCGTAATATGCCGGAGTCACCAGAA  >  NC_000913/3347029‑3347309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑