| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 543,094 | G→C | 7.4% | intergenic (+61/+167) | glxK → / ← allE | glycerate 2‑kinase 2/(S)‑ureidoglycine aminohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 543,094 | 0 | G | C | 7.4% | 119.6 / 3.2 | 49 | intergenic (+61/+167) | glxK/allE | glycerate 2‑kinase 2/(S)‑ureidoglycine aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (22/22); new base C (2/2); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01 | |||||||||||
GCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAG > NC_000913/542957‑543232 | gctgTGAAACCATTCTCTTCAACATCGCGCGAATTCTTGCCTGCGCCATTAAAATAGGTCAGGCAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCa < 1:122886/140‑1 (MQ=255) cggttgaaCTAATCGCTTCTACCGCGCGTGAAATCTTGCCTGCGTCATTAGAATAGGTCAGTGAATTATAAACTTATTCTTACCTTTAAAGCGGATGCGATTTATCTCGCTTTAGAGTGCAGTCCTCATGCCGGAGGCGGCAt < 2:771621/137‑1 (MQ=255) gttaaaCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTCACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGCGCCGTACCCATGCCGGAggcggccaga > 2:661400/4‑139 (MQ=255) gTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa > 2:356901/1‑143 (MQ=255) gTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa > 2:356902/1‑143 (MQ=255) gTGAAACCAATCTCTTCAACAGCGCGCGAAATAGTGGCTGCGCCATTAAAATAGGTCAGGGAAGTAAAAACTAACCCTTACCTTTAAAGCGGATGAGATTTATATCGCATAAGACCGCCGTACTCATGCCGGCTGCGGCGTGa > 2:887750/1‑143 (MQ=255) ccAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCTTGGAATTAAAATCTTACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAGTActg < 1:572558/143‑3 (MQ=255) cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCCTGAGTACCat > 1:769867/1‑143 (MQ=255) tctcTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCAtctc > 1:120098/1‑143 (MQ=255) ttCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttc > 1:475412/1‑143 (MQ=255) gcgcgcGAAAAACTGCCTGCGCCATTAAAATAGGTCAGCGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGaaaa < 2:1012480/143‑1 (MQ=255) cgcgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAACCTTCCTGCAAAt > 2:489096/1‑143 (MQ=255) gcgAAATATTGCCTGCGGCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGTAAATcgc < 2:907158/143‑1 (MQ=255) gcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGAGGCATGAGGACCATATCCTTCCTGAAAATcgc > 1:571667/1‑143 (MQ=255) tatTGCCTGCGCCATTAAAATAGGTCAGGGAATTACAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGAGGCGGCATGAGTACCGTATCCTTCCTGAAAATcggggcagt > 1:963996/1‑136 (MQ=255) tatTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCCCAAAt > 1:981136/1‑143 (MQ=255) gCCTGCGCCATCAAAATAGGTCAGGGAATTAAACACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCta < 1:213978/143‑1 (MQ=255) ccTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACACTTACACGAAGTACCGAGACTATTTTAGTTGTGGCACATCGCTGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtat > 1:744410/1‑143 (MQ=255) tGCGCCATTAAAATAGGTCAGGGAAGTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCCAAATCtatca > 2:997540/1‑141 (MQ=255) aTTAAAATATGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCa < 1:182568/143‑1 (MQ=255) aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATATATATAGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGAACCATATCCTTCCTGAAAATCGCGCAAAATCTATATATTGCa > 2:669375/1‑143 (MQ=255) tAAAATAGGTCAGGGCATTAAAAACTAACCCTTACCTCTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCaga < 2:919396/143‑1 (MQ=255) tAAAATAGGTCAGGGAGTTAAAAACTAACCCTTACCTTTAAAGCGGGTGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCaga < 2:420899/143‑1 (MQ=255) gaagagaGGTGAGGGAATTAAAGACTAACCCTTACCGTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTGCCTGAAAATCGCGCAAATTCTATATATTGCaga < 2:232296/137‑1 (MQ=255) aaaaTAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCagag < 1:390297/143‑1 (MQ=255) gAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATCTTGCAGAGATCATGTAGGCCt > 1:889488/1‑143 (MQ=255) aaCGAACCCTTCCCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcg < 2:652097/143‑1 (MQ=255) cTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaa < 1:601255/143‑1 (MQ=255) cTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaa < 1:48935/143‑1 (MQ=255) tcaCCCGTACCTTCGCAGCGCGTGGGATTGATATCGCATAAGACTGCAGTACTTATGCTGGCTCCGGCATGAGTACCATATCCTTCCTGAAAATTGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaag < 2:762061/141‑1 (MQ=25) gccTTACCTTTAACGCGGATGCGATTTATGTCGCATAAGCGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAgcgc < 2:268039/142‑1 (MQ=255) cccTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAgcgc < 2:590056/143‑1 (MQ=255) aaaGCGGACGGGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATg < 2:591158/143‑1 (MQ=255) cGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGATAc > 2:943939/1‑143 (MQ=255) gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGAGAAGCGCATCAGGCAATGTAAcga > 2:471337/1‑141 (MQ=255) gATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAAAGTTACaaaaaaa > 2:802820/1‑143 (MQ=255) aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCGATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAg > 1:471132/1‑143 (MQ=255) cGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGta > 2:87632/1‑143 (MQ=255) gCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGtat > 1:575311/1‑143 (MQ=255) gCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGtat < 1:489096/143‑1 (MQ=255) cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAACCGAAGCGCATCAGGGAATGTGACCAAAAAAGCTACCGtata > 2:57378/1‑143 (MQ=38) gaaGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATaaa < 1:879397/142‑1 (MQ=255) tggTACTCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATGAACCggggaa > 1:414817/3‑141 (MQ=37) cTCATGTCGGATGTGGCATGATTATCATATCCTTCCGGAAAATCGGGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCTCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATc < 2:109577/143‑1 (MQ=37) tCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAACAAAGCCACGGTATAAACCCTGGCAGAATcc > 2:422251/1‑143 (MQ=37) gtGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa < 2:194078/142‑1 (MQ=255) aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa < 2:301301/143‑1 (MQ=255) tggtggATTCGGCAGGAGTACCAGATCCTCCCTGAAAATGGCGTAAATTCTATATATTGCAGAGATCATGTAGGTCTGATAGGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAATCCGTGGCAAAATCCAAc < 2:140642/139‑1 (MQ=37) tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAAc > 2:380569/1‑143 (MQ=255) ggATGCGGCATGAGTACCATATCCTTCCTGAACATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACCAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAg < 2:556892/143‑1 (MQ=255) | GCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAG > NC_000913/542957‑543232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |