| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,123,110 | G→A | 100% | P77L (CCA→CTA) | ftsN ← | cell division protein FtsN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,123,110 | 0 | G | A | 100.0% | 149.5 / ‑6.7 | 56 | P77L (CCA→CTA) | ftsN | cell division protein FtsN |
| Reads supporting (aligned to +/- strand): ref base G (0/2); major base A (26/26); minor base C (0/2); total (26/30) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCG > NC_000913/4122971‑4123248 | ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGCAGCGCCAGCGTTCTTCTGGTTTtagtg > 1:455140/1‑143 (MQ=255)ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGATTCTGAGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg > 1:726557/1‑143 (MQ=255)ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTGTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 1:703273/143‑1 (MQ=255)ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCAGGCGTTTTCACTTCACCACCGGCAGAGGGGTCTGTGGGCGCACGCCCTCCCGGCGGGCGACTTTCCAGCTCGTTAATGTAGCGCCAGCGCTCCTCTGGTTGtagtg > 2:469349/1‑143 (MQ=255) aagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTc < 1:502901/143‑1 (MQ=255) cTGACGTTGTTCTGGTGTCAGTCGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGt < 1:752490/143‑1 (MQ=255) gttgttCGGGTGTCAGTTGCTCCGGCGCTTTCACTGCACCGCCGGCAGAGGGTTCTGTGGGCGCGCGCACTCCCGGCTGGTGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 2:231104/143‑1 (MQ=255) ggtgttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 2:135897/141‑1 (MQ=255) ggtgttCTGGTGTCAGTTGCGCCGGCGTTTTCGCTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 2:924244/141‑1 (MQ=255) gctgttCTTGTGTCTGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg < 1:419411/141‑1 (MQ=255) gttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTACTGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTCGTCCGTTTCCGGTc > 2:467947/1‑143 (MQ=255) ttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCa < 1:297223/143‑1 (MQ=255) gTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGCTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCtt > 1:389722/1‑143 (MQ=255) cTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCa < 1:967693/143‑1 (MQ=255) ccGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGc < 1:461121/143‑1 (MQ=255) ggCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGCCGGCCGGCTCCTGTCACTTTCTGGCTTTGCAGCGt > 1:686431/1‑143 (MQ=255) gTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCATGTAGCGCCGGCGTTCTTCTGGTTTTAGTGGTATTCAGTTTACGGTCACTTTCTGGCTTTGCAGCGtctc > 1:361728/1‑143 (MQ=255) gTTTTCACTTCACCACCGGCAGAAGGTTCTGTAGGCGCACGCACTCCCGGCTGACGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTGCTGGTTTTAGTGGTAGTCCGGTTCCGGTCACTTTCTGGCTTTGCAGCGtctc > 1:698316/1‑143 (MQ=255) ttcacttcacCACCGTCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa < 1:378204/143‑1 (MQ=255) ttcacttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACGCACTGGCAATAGAGCGTGTCGGa > 1:372794/1‑143 (MQ=255) ttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtctt < 1:713597/143‑1 (MQ=255) gcgccgccGGCAGGTGGTCCGGTGGGCGCACGCACTCCCGGCTGGCGACTGTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtcttc < 2:272650/137‑1 (MQ=255) caccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCttctttc < 1:549598/143‑1 (MQ=255) gCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCTTCTCGTACTCTTCTTTCTtgtg < 1:384901/143‑1 (MQ=255) cagcagGTTCTGTGGGCGCACGCACCCCCGCACGGCAGGTTGCCTTCTCTTTTATGTAGCGCCAGCGTTCTTCTGGTTTTCGTGGTCGTCCGTTTCCTGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGa < 2:715151/143‑1 (MQ=255) ttCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTaa < 2:826055/143‑1 (MQ=255) tCTGTGGGCGCACGCACTCCCGGCTGGCGAGTATCCAGCTCTTTCAGGTAGCGCCACCGTTCTCCTGGTTTGAGTGGTAGTCCGCTTCCGGTCGCTGGATGGCTTTGGAGCGACACGGACCCTTCTGTCCCGTGATGTGTAAt > 2:288037/1‑143 (MQ=255) cggtgCGCGCACGCACTCCCGCCTGGCGACTTTCCAGCTCTTTGATGTCGCGCCGGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg < 2:691938/141‑1 (MQ=255) cTGTCGGCGCACTCACTCCCGGCTGGCGACTTTCCAGCTCCTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCATCGTCTCGGCCTCTTCTTTCTTGTGATGCGTAATg < 1:194890/143‑1 (MQ=255) tgtgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGa > 1:292671/1‑143 (MQ=255) tgtgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGAGGCGTAATGa > 2:337835/1‑143 (MQ=255) gtgtGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTACTGTAGCGCCAGCTTTCCTCTAGTTGAGGCGCAAATTAGTATTTGTATACTTTCGGGCTTTGCAGCGACTCGGTCTTTTCTTTCTTGTGATGCGTAATGaa < 2:720317/143‑1 (MQ=255) tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAg > 1:714450/1‑143 (MQ=255) tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAg < 1:1014627/143‑1 (MQ=255) gcgcACGCACTCCCGGCTGGCGACTTTCCAGCTCTATAATGTAGCGCCAGCGTTCTCCTGTTTTTAGTGGTAGTCCGTTTCCGGCCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTAATGCAGtcc > 2:261394/1‑141 (MQ=255) cacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGa < 1:479824/143‑1 (MQ=255) tCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTcc < 2:760957/66‑1 (MQ=255) tCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTcc > 1:760957/1‑66 (MQ=255) ctggCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCGTCGGGTTTTAGTGTTATTCCGTTTCCGGTCCCTTTCTGGCTTTGCAGGGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAGGTACAGACGACCGATa > 2:439225/3‑143 (MQ=255) ccGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCCCCGaca > 2:534245/1‑141 (MQ=255) tGGTGATTTTTCAGTTTTTTAATGTAGCGCCAGGGGTCGACGGGCGAGAGTGGTAGTCCGTGTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGt < 1:25940/143‑1 (MQ=255) tGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGCGATGCGTAATGCCGTACAGACCCCCGATAACggc > 2:971227/1‑142 (MQ=255) aCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTGTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACaa < 1:424136/143‑1 (MQ=255) aCTTTCCAGCGCTTTTATGTAGCGCCAGCGTTCTTCTGGTGTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACaa < 2:639935/143‑1 (MQ=255) aGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTAGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTAGTGAAGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc > 1:690064/1‑143 (MQ=255) tctTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACggcggc > 1:675900/1‑143 (MQ=255) tctTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACGTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGAGTAGTGAAGTACAGACCACCGATAAAGGTCACAAGAACggcggc > 1:346631/1‑143 (MQ=255) tttACTGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGgcag < 1:197743/143‑1 (MQ=255) tAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTAATGCAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGc > 2:253110/1‑143 (MQ=255) ggtccAGTGTTTTTCTGGTTTGCGTGGTAGTCCGTTTCCGGCCACTTTCTGGCTTTGCAGCGGCCCGGACTCTTCTTGCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa < 2:352391/140‑1 (MQ=255) gcgcCAGCGGTCGTCTGGTTTTAGTGGTAGTCCGTTACCGGGCACATTCTGGCTTTGCAGCGTCGCGGACTCTTCTTTCTTGTGATGCGTAATGACGTAGGGACCACCGATAAAGGACCCCAGTGCGGCGGCAGCAATTGCgg > 2:634371/1‑142 (MQ=255) cgcCTGCGTTCTTCTGGTTGCAGTTGCAGTCCGGTTCCTGTCGCTTTCTGGCTTTGCAGCGTCTCGGTCTCTTCTTTCTTGTGATGCGTAGTGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGAc < 2:235363/143‑1 (MQ=255) cgcCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTCCTTTCTTGTGATGCGTAATGAAGGACAGACCACCGATAAAGGTAACAGGAACAGCGGCAGCAATACCGAc > 2:880745/1‑143 (MQ=255) tgctttcatcTCGTTTTATTTGTAGTCCGTTTTCGGTCACTTTCTGGCTTTGCAGCGGCTCGGTCTCTTCTTTCTTTTGATGCGTGATGAAGTACAGACCATCGATTAAGGTCACAAGTACGGCGGCAGCAATAGCGACCATa < 2:977777/139‑1 (MQ=255) gCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAg > 1:877048/1‑143 (MQ=255) tcttctGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCggg < 1:394006/143‑1 (MQ=255) ttctGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCGCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGgag > 2:247299/1‑143 (MQ=255) tctGGTTTTAGTGGTAGTCCGTTTCCGGTCGCTTTCTGGCTTTGCAGCGTCTCGGGCTCTTCTTTCTTGTGATGCGTTATGACGTACAGACCACCGATAAGGGTCACACGAACGGCGGCAGCGAGAGCGACCATGGCGGgcga > 2:973244/1‑143 (MQ=255) ggTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAAc > 1:354482/1‑143 (MQ=255) ttttAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACAGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCg > 1:815652/1‑143 (MQ=255) | TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCG > NC_000913/4122971‑4123248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |