Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	696,470	C→T	100%	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	99.7 / NA	37	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/26);  total (11/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696331‑696606                                                                                                                                            |                                                                                                                                         gTCGCATCCGACATTGAAGGAAAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                       <  1:265084/143‑1 (MQ=255)  tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTCTTTTAATGTGGCTGGGGGGCGAGGACTCGAACCCCGGAATGCCGGACTTAGaa                                                                                                                                      >  2:630105/1‑143 (MQ=255)     cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTAGACCCTCGGAATGGCGGGATTAGAATcc                                                                                                                                   >  2:349297/1‑143 (MQ=255)        ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCTGGAATTAGAATCCGGt                                                                                                                                <  1:615646/143‑1 (MQ=255)        ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                >  2:51940/1‑143 (MQ=255)              ttGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCGTTTTTACTGTAGCTGGGGTACGCGCATTCGACCCTCGGAATGCCGGAATTAGAATCCGGGGCGttg                                                                                                                          >  2:419659/1‑142 (MQ=255)                                     tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                   <  2:865654/143‑1 (MQ=255)                                      ggtcttCGACATTGAATGAACGCAGAAGAGCAGAAGGCTCGCCGAAGCGAGCGTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                  <  2:900697/139‑1 (MQ=21)                                      cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTGATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                          <  1:563105/135‑1 (MQ=255)                                      cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTGATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                          >  2:563105/1‑135 (MQ=255)                                      cGCATTCCACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                  <  1:59535/143‑1 (MQ=255)                                         aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCGTGGCGGTACCCCGACggggcgg                                                                                               >  2:802271/1‑140 (MQ=25)                                          tcggACAATGAAGGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                              <  1:383859/140‑1 (MQ=255)                                           gcgacggtgaagggaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc                                                                                             <  2:151504/136‑1 (MQ=21)                                                   gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg                                                                                     >  1:675677/1‑143 (MQ=255)                                                            cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTTCCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCt                                                                            <  1:826277/143‑1 (MQ=255)                                                             aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAGGGTAAGCGTCtt                                                                           <  2:387941/143‑1 (MQ=255)                                                             aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  1:611369/143‑1 (MQ=255)                                                             aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  1:691359/143‑1 (MQ=255)                                                               aaccgCAAAAAGCTCGCCGCAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                         <  1:931480/139‑1 (MQ=255)                                                               aacaGCAAACAGCGCGCCGAAGAGAGCTTTTTTAATGCTGCTGGTGTACGAGGATTCGAACCTCGGAATGCCCGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                         <  1:940278/140‑1 (MQ=21)                                                               aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCGTGa                                                                         >  1:114819/1‑143 (MQ=255)                                                                     aacaaGCTCGCCGAAGCGATCTTTTTTAATGTGCCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                   <  1:336182/140‑1 (MQ=255)                                                                     aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTGCGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGATTTACAAGGTGAGAGGCTTGAAGAAAt                                                                   >  1:699750/1‑143 (MQ=21)                                                                     aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                   >  1:884084/1‑143 (MQ=255)                                                                            gcGCCGAGGCGAGCTGGTTTAATGTGGCTGGGGTACGAGGAGTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                            <  1:560103/142‑1 (MQ=25)                                                                             cGCCGAGGCGAGCTTTTTTAATGCGGCTGGTGTACGAGGATTCGCACCTCGCAGTGCCGGACTTCGATTCCGGTGCCTTTCCGCTTGGCGATACCCCAACTGTGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                           <  2:258312/143‑1 (MQ=255)                                                                                       atcTTTTTCAATGTGGCTGGGGTACGAGGATTCGAACCTCACAATGTGGGTAGTGGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                 <  2:760851/141‑1 (MQ=25)                                                                                         cTTTTTTAAGCTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                               <  2:674793/143‑1 (MQ=255)                                                                                                agtgtgGCTGGGGTAGGAGGATTCGAACCTTGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                        <  2:1107/141‑1 (MQ=255)                                                                                                 atctgGCTGGGGTACGATGATTCGAACCACTGCATGCCGCAATTAGACTCCGCCGCCTTACCGCTTGGCGATACCCCAACTGGGTACACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                       <  2:95833/140‑1 (MQ=25)                                                                                                          gggTAGGAGGAGTCGAACCCCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                              <  1:602724/143‑1 (MQ=255)                                                                                                                   gATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                     <  1:990831/143‑1 (MQ=255)                                                                                                                                      cGGAATTAGAGTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTTCAAGGTAAGCGTCTTGATTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg  <  1:765931/143‑1 (MQ=25)                                                                                                                                      cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                               >  2:702247/1‑98 (MQ=255)                                                                                                                                      cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                               <  1:702247/98‑1 (MQ=255)                                                                                                                                      cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg  <  1:726929/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696331‑696606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑