Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 699,556 C→T 9.0% intergenic (‑379/+18) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913699,5560CT 9.0% 136.2 / 4.0 54intergenic (‑379/+18)asnB/umpHasparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (19/29);  new base T (3/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

GATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTG  >  NC_000913/699414‑699689
                                                                                                                                              |                                                                                                                                     
gATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTcc                                                                                                                                       <  2:312491/143‑1 (MQ=255)
 aTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCg                                                                                                                                      <  2:567596/143‑1 (MQ=255)
   ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCa                                                                                                                                    <  2:1009534/143‑1 (MQ=255)
    tGTTCATGCCGGATGAGGCGTTAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAg                                                                                                                                   <  1:781552/143‑1 (MQ=255)
       tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCCCGTCCGCAGACg                                                                                                                                >  1:918458/1‑143 (MQ=255)
         aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTCCAGGAGCTGCGTAGGCCTGATAAGCCCAGCGCATCAGGCAGTTTGGCGTTTGTCATCAAAGCCAACCACGTCCGCAGCCggg                                                                                                                              >  2:45544/1‑141 (MQ=21)
            ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGtt                                                                                                                           <  2:684652/143‑1 (MQ=255)
              ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGc                                                                                                                         <  2:837129/143‑1 (MQ=255)
                    ggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCACTATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGTGCCTACCACGTCCGCAGACGTGGTTGCTATTCa                                                                                                                   <  2:122501/143‑1 (MQ=255)
                     gcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAATCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTGCGCAGCCGTGGCTGCTCTTccg                                                                                                                  >  1:429416/1‑141 (MQ=21)
                         gAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCACTCTAATGCATGAGCTGCGAAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGATCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa                                                                                                              <  1:904597/143‑1 (MQ=25)
                          aaCGCCTTATCCGGCCTACAAAATCATGAAAATAACTTAATTTTCAGGAGCTGCGTAGGCTTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAAc                                                                                                             <  2:446655/143‑1 (MQ=25)
                              ccTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCg                                                                                                         >  2:271787/1‑143 (MQ=255)
                                      ggCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc                                                                                                 <  1:490153/143‑1 (MQ=255)
                                        ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGa                                                                                               <  2:12571/143‑1 (MQ=255)
                                          tACAAAAGCAGGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGCTAACGTCGATTTCAGCGACt                                                                                             >  2:221551/1‑143 (MQ=255)
                                              aaaGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCCGGCAGTTTGGCGTTTGTCATCAGACCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACg                                                                                         <  2:502519/143‑1 (MQ=255)
                                                      aaaaTTCAATAGATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTACGATAACGGCTATTTCAGCGACTGACGGGTAAATc                                                                                 <  2:888578/143‑1 (MQ=37)
                                                               tatatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                                        >  1:167753/1‑143 (MQ=255)
                                                                 gatggCAGGAGGTGCGGAGGCCGGATAAGCGTAGCGCATGAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGGCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                                      <  2:514298/139‑1 (MQ=255)
                                                                   ttGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGa                                                                    >  1:919843/1‑143 (MQ=255)
                                                                     gCACGAGCTGCGTAGGCCTGATAAGCGTAGCGCACCAGGCAGTTTCGCGCTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGaaa                                                                  <  1:846658/143‑1 (MQ=255)
                                                                      cttgAGCTGCGTAGGCCTGATAAGCGGAGCGCATCAGGGAGGTTGGCGTTTGTCATCAGAGCCAACCACGTTCGCAGACGTGGTTGCTATACAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAg                                                                 <  2:866875/140‑1 (MQ=255)
                                                                               cGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATCTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGt                                                        >  2:993708/1‑143 (MQ=255)
                                                                                 tAGGCCTGATAGGCGTAGCGCATCAGGCAGTTTGGTGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCg                                                      <  2:90751/143‑1 (MQ=255)
                                                                                 tAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCg                                                      >  1:589998/1‑143 (MQ=255)
                                                                                   gggcTGATAGGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGat                                                    <  2:961537/140‑1 (MQ=255)
                                                                                           atgCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTTGTTGCTATTCAGATAACGTCGATTTTAGCGACTGTCGTGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                                            <  2:70505/141‑1 (MQ=255)
                                                                                           aaGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                                            <  1:768918/143‑1 (MQ=255)
                                                                                           aaGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                                            >  2:527473/1‑143 (MQ=255)
                                                                                            agagTAGCGCATCAGGCAGTTTGGCGTTTGTTATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTTGATTTCAGCGACTGACGGTTAAATCCAGCTGGGGCTGAAAGGCATACTGTCGATATCGTCGAg                                           <  2:771648/140‑1 (MQ=255)
                                                                                                  gcgcATCATGCAGTTTGGCGTTTGTCATCAGAGCCACCCACGTCCGCAGACGTGGTTGCTATTCAGATCACGTCGATTTAAGCGACTGACGGGTAAATCCAGCTGAGGCGGAAGGGCATACTGTCGCTATAGCCGCGcgacga                                     >  2:998891/1‑143 (MQ=255)
                                                                                                  gcgcATCAGGCAGTTTGGCGTTTGTCATCAGCGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCCGCGACTGACGGGTAAGTCGAGCTGGGGCGGAAAAGCATACTGTAGATAGCGTAGAGcggcga                                     >  2:829642/1‑143 (MQ=255)
                                                                                                   cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcgcagca                                    >  2:596420/1‑138 (MQ=255)
                                                                                                    gcATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                                   <  2:731026/143‑1 (MQ=255)
                                                                                                    gcATCAGGCAGTCTGGCGTTTGTCATCAGAGCCACCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                                   <  1:80860/143‑1 (MQ=255)
                                                                                                    ccaggcggCAGTTGGGCGTGTGTCGTCAGCGCCAACCAGGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAGCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                                   <  1:80859/137‑1 (MQ=255)
                                                                                                          ggCAGTTCGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAg                             <  1:884735/143‑1 (MQ=255)
                                                                                                             aGTCTGGCGTTTGTCATAAAATCCATCTGCGTCCGCAGACGTGGTTGCGATGCAGATAACGTCCATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaaa                          <  2:657813/143‑1 (MQ=255)
                                                                                                              gTTTGGCGTTTGTCATCAGAGGCGAGCAGGTCCGCAGACGTGGTTGCGATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAg                         <  1:271787/143‑1 (MQ=255)
                                                                                                               tttGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGCTTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATAGTGTGGATATCATCGAGCGACGAAACACCAGAAAga                        >  1:686252/1‑142 (MQ=255)
                                                                                                                  ggCTTTTGTTAGCCGAGCCAACCACGTCTGCGGACGTTGTTGTTCTTCTGTTTATGTAGATCTCTGTGTCTGACGGGTAAATCCAGCTGGGGCGGAACGGCATCCTGTCGTTATCGTCGAGCGACGAAACACCAGAAAGCAcc                     <  2:258964/143‑1 (MQ=255)
                                                                                                                     gTTTGTCATCAGAGCCAACCACGTCAGCAGACGTGGGTGCTATTCAGATAAAGTCGACTTCAGCGACTGACGGGTAAATCCAGCTGGAGCGGAAAGGCATACTGTCGGTATCGACGAGCGACGAAACACCAGAAAGCGCCaca                  >  2:646084/1‑141 (MQ=255)
                                                                                                                         gTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAACGCACCAGAATCg              >  2:675239/1‑143 (MQ=255)
                                                                                                                          tcatcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGCCGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGGGCCACGAAACACCAGAAAGCACCAGACTcca             >  2:863167/1‑141 (MQ=255)
                                                                                                                           catcaGAGCCAGCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCGCCAGAATCGtc            <  2:256779/143‑1 (MQ=255)
                                                                                                                               agtgCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa        <  2:181984/140‑1 (MQ=255)
                                                                                                                               agagCCAGCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa        <  2:918458/143‑1 (MQ=255)
                                                                                                                               agagCCAACCACGTCCGCAGACGTGGATGCTATTCAGTGGACTTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa        <  1:184891/143‑1 (MQ=255)
                                                                                                                                   ccAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAACGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGTCACCAACAGAACCGTCGCCAGAca    >  1:168797/1‑142 (MQ=255)
                                                                                                                                   ccAACCACGTCCGCAGACGTGGTTGCTAGTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGACCGGCATACTGTCGATATCGTCGAGCGACGACACACAAGAACGCACCAGCATCGTCTCCAGccc    >  2:876783/1‑143 (MQ=255)
                                                                                                                                     aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAACGCACCAGAATCGTCTCCAGAcctg  >  1:251778/1‑143 (MQ=255)
                                                                                                                                     aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctg  >  1:905687/1‑143 (MQ=255)
                                                                                                                                     aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGACTCCAGCGACTGACGGGTATATCCATCGGGGGCGCCAAGGAATCCCGCCGATATCGACGAGCGACGACCCACCAGCACGCACCACAATAGTccccccaaccc  >  2:494240/1‑136 (MQ=255)
                                                                                                                                     aaCCACGTCCGCAGACGTGGATGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGCCGGAAAGGCATACTGTCGATATCGTCGCGCGACGACACACCCGAAAGCACCACAAACGTCGCGAGAccgg  >  2:590859/1‑141 (MQ=255)
                                                                                                                                              |                                                                                                                                     
GATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTG  >  NC_000913/699414‑699689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑